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Offlinenewtomyc
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what do you guys think?
    #26340922 - 11/23/19 06:19 PM (4 years, 2 months ago)

So I had some cultures store in my garage fridge in an insulated lunch box.  Well recently our weather here got below freezing for a couple days and I completely forgot to move the box inside (just spaced it).

I'm pretty sure my plates got frozen but there was growth after I set them out to room temp for a few days. 

Now I'm almost positive that this is bacteria but I would be remissed not to at least ask if anything is redeemable. There was 15 clean plates and they all looked about the same as this.


This is one of the MSRW plates on PDA. The last image is 30x mag of the new growth.

Here is a transfer plate from some of that new growth.

All these are on LMA and last pic is 30x mag.

Now the transfer samples are not from that top plate that is pictured, but all the plates were so similar, I just wanted to show an example.

Like I stated in opening paragraph, pretty sure it's bacteria.  I would like some notes and will probably turn this into a study on bacterial for myself (just ordered a microscope for my birthday). 

Thanks for any good advice/notes/comments/learn opt.


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JJ

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OfflineKryptos
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Re: what do you guys think? [Re: newtomyc]
    #26340928 - 11/23/19 06:21 PM (4 years, 2 months ago)

Bacteria generally looks like melted ice cream, especially on agar.

Those don't look bacterial to me.


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Re: what do you guys think? [Re: Kryptos]
    #26340937 - 11/23/19 06:25 PM (4 years, 2 months ago)

Quote:

Kryptos said:
Bacteria generally looks like melted ice cream, especially on agar.

Those don't look bacterial to me.



:whathesaid: looks like myc to me


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Offlinenewtomyc
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Re: what do you guys think? [Re: Kryptos]
    #26340940 - 11/23/19 06:26 PM (4 years, 2 months ago)

Quote:

Kryptos said:
Bacteria generally looks like melted ice cream, especially on agar.

Those don't look bacterial to me.




My concern is the transfers are so shinny/translucent and not white.

Maybe a mold that I didn't realize I had.

thanks for the input!!


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JJ

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Re: what do you guys think? [Re: newtomyc] * 1
    #26340961 - 11/23/19 06:35 PM (4 years, 2 months ago)

The outer part is growing again, right? Looks like myc to me. If the dish was clean & wrapped, I doubt the freezing introduced bacteria or contam fungi. The color could be just burst cells whose juices react with stuff or are being eaten by the survivors. If transfers from the growing front look right and smell right...


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Offlinenewtomyc
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Re: what do you guys think? [Re: curious.psychonaut]
    #26340985 - 11/23/19 06:45 PM (4 years, 2 months ago)

Quote:

curious.psychonaut said:
The outer part is growing again, right? Looks like myc to me. If the dish was clean & wrapped, I doubt the freezing introduced bacteria or contam fungi. The color could be just burst cells whose juices react with stuff or are being eaten by the survivors. If transfers from the growing front look right and smell right...




I think I'll put them to grain and see what's what.

Do you smell your plates? I don't get that close to him to do that. I will smell once they callin eyes those grades and see if it's sour.

Thanks for the input I appreciate it.


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JJ

Draw unto others as they have been drawn to you.... WSP


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Offlinecurious.psychonaut
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Re: what do you guys think? [Re: newtomyc] * 1
    #26341041 - 11/23/19 07:14 PM (4 years, 2 months ago)

Quote:

newtomyc said:
Do you smell your plates?




Lol, I always do. They smell so delicous, esp. when the scalpel is still hot and cooks the mycelium a bit. A whiff of that often travels through the SAB holes, but I also always smell used a2g plates directly, rubbing some of the remaining mycelium with my finger after all the sterile work is done. There are subtle differences in smell between the three lineages I'm currently culturing from the same print. Maybe I'm imagining things, but I think I can make them out even in the grain jars.


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My LAGM2020 grow log



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Offlineverum subsequentis
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Re: what do you guys think? [Re: curious.psychonaut]
    #26354647 - 11/30/19 09:06 PM (4 years, 2 months ago)

:havesomescience:


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