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InvisibleRogerTheRetard
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Registered: 07/28/17
Posts: 2,545
Loc: Auckland, NZ Flag
Media bottle/petri dishes vs PP5 plastic no pour
    #26340769 - 11/23/19 05:16 PM (4 years, 2 months ago)

I have a 23 quart presto begging to be used. (I want mushrooms asap)

But I can not for the life of me decide with style of agar plates I want to get into making.
:header:

I have searched the forums for answers, but still I can not seem to make my mind up. Seems to just be personal preference from what I gather.
Some people vouch pouring petri dishes, where as some people vouch no pour agar as the supreme reign. :shrug:

I'm leaning more towards the PP5 plastic no pour route.
Anyone care to solidify my lean towards the PP5 no pour route?

Or perhaps you hate no pour agar and only ever pour petri dishes with your media bottle?

I'm just kinda stuck at this point, it's a kerfuffle.

Someone make my mind up for me surely? I'm stuck staring at the wall contemplating which route I take over here and it's boring.


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:hitler::mushroom2:angulospora:heart:subaeruginosa:heart:subsecotioides:heart:tasmaniana:mushroom2::hitler:



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OfflineKazoo_bard
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Registered: 09/27/19
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: RogerTheRetard]
    #26340776 - 11/23/19 05:18 PM (4 years, 2 months ago)

Smoke a bowlski and give a coin a flip.  Or, get the stuff to do pour plates. I'm going with pour plates because they're gonna stack nicer in my mini fridge and I'll be able to keep a LOT more active my samples for grain noccs.


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InvisiblemushboyMDiscord
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: RogerTheRetard] * 1
    #26340789 - 11/23/19 05:23 PM (4 years, 2 months ago)

For me no pours were easiler to learn but petris are all sciencey and shit:nerd: but harder to get down. Pouring, media bottles ect..


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InvisibleRogerTheRetard
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Registered: 07/28/17
Posts: 2,545
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: Kazoo_bard] * 1
    #26340791 - 11/23/19 05:23 PM (4 years, 2 months ago)

Oh mate, I have been smoking enough bowlskis...

I need to lay off the bolwskis, lads a bit addicted to said bowlskis.

Lads a procrastinator and a lazy cunt. Must be the bowlskis...


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:hitler::mushroom2:angulospora:heart:subaeruginosa:heart:subsecotioides:heart:tasmaniana:mushroom2::hitler:



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InvisibleRogerTheRetard
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Registered: 07/28/17
Posts: 2,545
Loc: Auckland, NZ Flag
Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy] * 1
    #26340796 - 11/23/19 05:26 PM (4 years, 2 months ago)

Quote:

mushboy said:
For me no pours were easiler to learn but petris are all sciencey and shit:nerd: but harder to get down. Pouring, media bottles ect..




Hard out this is what I feel.

I reckon I'm a noob so I'll just go with no pour agar to kick things off and dip my toes into working with agar.

Then perhaps after that I may gain some confidence and go for the no pour option.

Or do I just pick up my nutsack and get the petri dishes.

Contemplation hits heavy.


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:hitler::mushroom2:angulospora:heart:subaeruginosa:heart:subsecotioides:heart:tasmaniana:mushroom2::hitler:



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InvisiblemushboyMDiscord
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: RogerTheRetard]
    #26340803 - 11/23/19 05:28 PM (4 years, 2 months ago)

Start with no pours. Learn petris while you drown in a sea of shrooms you grew from the no pours.


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InvisibleRogerTheRetard
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: RogerTheRetard]
    #26340805 - 11/23/19 05:30 PM (4 years, 2 months ago)

And fuck, I don't have a flowhood so I'm leaning towards no pour.

Flowhood seems more suitable for petri dish pouring.

I'll be making a SAB

Perhaps I should just go the noob route first time around and do the pp5 plastic no pour plates.

My inner decision maker leans more towards the easy pp5 plastic container no pour option.


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:hitler::mushroom2:angulospora:heart:subaeruginosa:heart:subsecotioides:heart:tasmaniana:mushroom2::hitler:



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InvisibleRogerTheRetard
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26340808 - 11/23/19 05:31 PM (4 years, 2 months ago)

Quote:

mushboy said:
Start with no pours. Learn petris while you drown in a sea of shrooms you grew from the no pours.




I like you.

:winner:


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:hitler::mushroom2:angulospora:heart:subaeruginosa:heart:subsecotioides:heart:tasmaniana:mushroom2::hitler:



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OfflineKazoo_bard
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26340815 - 11/23/19 05:34 PM (4 years, 2 months ago)

I'm going straight to dishes because I dont want to get comfy with the easy way. If I'm gonna do this, I want to go the fucking mile.  However, it's your choice.  The real question is how many cultures do you plan on keeping at once?  I'm still a noob so dont take this as gospel, but the no pours seem to take up way more room. If you're only doing a couple dishes, that's one thing, but if you're trying to grow out enough to nocc up 100 jars? Maybe look to dishes?
I'm also beating the petri drums since I already bought the stuff for it.
I'm gonna use a SAB and see where that gets me. Enough TCs have made it clear that I should be fucking up incredibly if I cant do it with a SAB.
Shout out to verum for now making me thing of Hillary Clinton's dirty vagina everytime I think of agar now


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InvisibleRogerTheRetard
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26340822 - 11/23/19 05:38 PM (4 years, 2 months ago)

My mind has been made up for me.

Quote:

mushboy said:
Start with no pours. Learn petris while you drown in a sea of shrooms you grew from the no pours.




Case closed.


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:hitler::mushroom2:angulospora:heart:subaeruginosa:heart:subsecotioides:heart:tasmaniana:mushroom2::hitler:



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Offlinecurious.psychonaut
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26340826 - 11/23/19 05:39 PM (4 years, 2 months ago)

When I started with this hobby (last month), I went to a lab supplier and got like 80 plastic petris (along with media bottles, etc.). I thought it was a lot. Made 16 spore plates, then 17 transfers, then 34 transfers. Then I bought a box of 500 triple-vented 90mm petris. Moar and moar transfers, culturing for different traits: fluffy mycelium, ropey mycelium, submerged mycelium, crazy mycelium. This shit is fun. I have plates in my fridge, plates that I've set aside for pinning, plates with various contams battling the cubes. I can't imagine where I'd put all these if they were in larger containers. Visibility is great with plates, also resulting great photos. Get plates.



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InvisibleRogerTheRetard
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Loc: Auckland, NZ Flag
Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: curious.psychonaut]
    #26340933 - 11/23/19 06:23 PM (4 years, 2 months ago)

That's awesome!!!

I know that I could just start with petris from the get-go.
I'm a retard and a lazy stoner and a noob.

So I'll start with no pour, then evolve into pouring petris.

Those no pour agar plates just look effortless I wana start there to be honest.

As I develope skill and get a feel for things, I will scrap the pp5 plastic and go for the standard media bottle/petri dish route.

Does this noob really want to be pouring agar from a bottle into petri dishes?
Not really to be honest. The no pour route seems way easier for my noob ass to accomplish.


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:hitler::mushroom2:angulospora:heart:subaeruginosa:heart:subsecotioides:heart:tasmaniana:mushroom2::hitler:



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OfflineChippaJone
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: RogerTheRetard]
    #26340977 - 11/23/19 06:42 PM (4 years, 2 months ago)

Where is the best place to buy the screw on top containers?  Walmart is out of stock online and in the stores I went to.  Is there another brand of screw tops available online or in stores?


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InvisibleRogerTheRetard
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Registered: 07/28/17
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: ChippaJone]
    #26340987 - 11/23/19 06:46 PM (4 years, 2 months ago)

I'll be fucked if I know, I live in New Zealand.

We don't even have glad mini rounds here, not sure about the ziplock screw tops.

I'm gona have to decide on a  brand of pp5 plastic small containers to make agar plates out of myself here...


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InvisibleSpunkyMonkey88
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26341012 - 11/23/19 06:58 PM (4 years, 2 months ago)

Quote:

mushboy said:
Start with no pours. Learn petris while you drown in a sea of shrooms you grew from the no pours.




I'm currently 2 transfers in using nopours and Im really hoping there is some truth to that statement!


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InvisibleSir Pentinite
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: RogerTheRetard]
    #26341038 - 11/23/19 07:13 PM (4 years, 2 months ago)

Quote:

RogerTheRetard said:
And fuck, I don't have a flowhood so I'm leaning towards no pour.

Flowhood seems more suitable for petri dish pouring.

I'll be making a SAB

Perhaps I should just go the noob route first time around and do the pp5 plastic no pour plates.

My inner decision maker leans more towards the easy pp5 plastic container no pour option.




A SAB works just fine for pouring agar.

One thing to consider with no-pours is that they are often harder to photograph clearly. That's a problem when asking questions of the community that require seeing what you have growing on the plate.


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"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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InvisibleSpunkyMonkey88
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: Sir Pentinite]
    #26341066 - 11/23/19 07:27 PM (4 years, 2 months ago)

Quote:

Sir Pentinite said:
Quote:

RogerTheRetard said:
And fuck, I don't have a flowhood so I'm leaning towards no pour.

Flowhood seems more suitable for petri dish pouring.

I'll be making a SAB

Perhaps I should just go the noob route first time around and do the pp5 plastic no pour plates.

My inner decision maker leans more towards the easy pp5 plastic container no pour option.




A SAB works just fine for pouring agar.

One thing to consider with no-pours is that they are often harder to photograph clearly. That's a problem when asking questions of the community that require seeing what you have growing on the plate.




Ya they really suck for pics but I just wipe my phone down with ISO and take pics in the SAB while tilting the trays so I dont have to hold my phone above them...


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InvisibleRogerTheRetard
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: Sir Pentinite]
    #26341125 - 11/23/19 07:55 PM (4 years, 2 months ago)

Quote:

Sir Pentinite said:
Quote:

RogerTheRetard said:
And fuck, I don't have a flowhood so I'm leaning towards no pour.

Flowhood seems more suitable for petri dish pouring.

I'll be making a SAB

Perhaps I should just go the noob route first time around and do the pp5 plastic no pour plates.

My inner decision maker leans more towards the easy pp5 plastic container no pour option.




A SAB works just fine for pouring agar.




Yeah, apparently pouring plates in a SAB is easy enough.
I'm just going to go with PP5 plastic first time round. :shrug:

I'll get the media bottle and plates next time round, the PP5 plastic plates should suffice for a beginner honestly.

Plus this way I don't have to fuck with pouring agar in a SAB and shit cooling down and having heaps left that I have to re-heat and all that mess.

Guess they call the no pour plates easy for a reason.


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Offlinerido
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: curious.psychonaut]
    #26341134 - 11/23/19 08:01 PM (4 years, 2 months ago)

Quote:

curious.psychonaut said:
When I started with this hobby (last month), I went to a lab supplier and got like 80 plastic petris (along with media bottles, etc.). I thought it was a lot. Made 16 spore plates, then 17 transfers, then 34 transfers. Then I bought a box of 500 triple-vented 90mm petris. Moar and moar transfers, culturing for different traits: fluffy mycelium, ropey mycelium, submerged mycelium, crazy mycelium. This shit is fun. I have plates in my fridge, plates that I've set aside for pinning, plates with various contams battling the cubes. I can't imagine where I'd put all these if they were in larger containers. Visibility is great with plates, also resulting great photos. Get plates.






I thought I was being smart by using the little 4 oz jars for agar. Great as no-pour since they're reusable, I thought. Well for one they're a pain to open/close (lid + ring vs just lifting petri lid gently), and two, it's next to impossible to see what's going on in there. I think I'm going to re-purpose the jars into candles for christmas or something because they're not getting used for agar again any time soon.

So yes, petri dishes all the way!


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OfflineBeerusthedestroyer
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26341138 - 11/23/19 08:04 PM (4 years, 2 months ago)

Quote:

mushboy said:
Start with no pours. Learn petris while you drown in a sea of shrooms you grew from the no pours.



This is exactly what i did. Now that i know wtf i am doing i prefer pouring my plates


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InvisiblemushboyMDiscord
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: curious.psychonaut]
    #26341139 - 11/23/19 08:05 PM (4 years, 2 months ago)

Quote:

curious.psychonaut said:





dont use the lid just work on the table surface


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Offlinecurious.psychonaut
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26341187 - 11/23/19 08:27 PM (4 years, 2 months ago)

Quote:

mushboy said:
Quote:

curious.psychonaut said:





dont use the lid just work on the table surface




Just for better comfort or is there a functional difference I'm missing? My thinking was that varnished wood is hard to clean properly, so sliding petris around is probably going to stir up some spores and what not in the air. (Also, I want to be kind to the table, it's used for eating.)


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My LAGM2020 grow log



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OfflineBeerusthedestroyer
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: curious.psychonaut]
    #26341193 - 11/23/19 08:31 PM (4 years, 2 months ago)

Probably because most lids (ime) will buckle under the weight of your media jars/petris/etc and make shit awkward. Dig?

Use empty half pint jars or empty petris to keep your stuff off the floor of the sab. Thats what i do... I never let anything touch the sides, roof, or floor of my sab unless its there to set shit on top of..


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Edited by Beerusthedestroyer (11/23/19 08:32 PM)


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InvisiblemushboyMDiscord
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: curious.psychonaut]
    #26341196 - 11/23/19 08:31 PM (4 years, 2 months ago)

get some kind of metal rack to elevate your tools and dishes. i use this old shit i found under the sink when i moved in.


i found that the lid when touched can cause the sab to vibrate/disturb the air. if it works certainly dont 'fix' anything just sharing some insights:stoned:


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OfflineBeerusthedestroyer
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26341198 - 11/23/19 08:33 PM (4 years, 2 months ago)

Quote:

mushboy said:
get some kind of metal rack to elevate your tools and dishes. i use this old shit i found under the sink when i moved in.


i found that the lid when touched can cause the sab to vibrate/disturb the air. if it works certainly dont 'fix' anything just sharing some insights:stoned:



Nice rack dude. I need something like that. When i get off work im gonna slither over to walmart and see what i can find


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InvisibleCaps McGee
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: Beerusthedestroyer]
    #26341216 - 11/23/19 08:39 PM (4 years, 2 months ago)

Ask yourself: "Am I a scientist?" If the answer is yes, POUR PLATES!!!


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Offlinecurious.psychonaut
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26341248 - 11/23/19 09:01 PM (4 years, 2 months ago)

Quote:

mushboy said:
i found that the lid when touched can cause the sab to vibrate/disturb the air. if it works certainly dont 'fix' anything just sharing some insights:stoned:




Ah, very good point, thanks! My contam rate is still around 10%, 135 plates in, so I am looking for things to fix!


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InvisibleRogerTheRetard
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: curious.psychonaut]
    #26341277 - 11/23/19 09:25 PM (4 years, 2 months ago)

Quote:

Beerusthedestroyer said:
Quote:

mushboy said:
Start with no pours. Learn petris while you drown in a sea of shrooms you grew from the no pours.



This is exactly what i did. Now that i know wtf i am doing i prefer pouring my plates




I'm sure I'll be the same. :thumbup:

I'm gonna just get around 20 or so little pp5 containers so I can just get a few things running to kick thing off.

I'll get a media bottle and just run petri dishes after that.

But as a noob, I want to start with the noob agar tek.
Just so I dont have to pour anything ykno


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OfflineFreeCanadianHugs
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: RogerTheRetard]
    #26341464 - 11/24/19 12:31 AM (4 years, 2 months ago)

I prefer to pour my plates. But try either method and if it works for you and your cultures that's what matters right?


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Offlinenarmaduke
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: mushboy]
    #26341467 - 11/24/19 12:38 AM (4 years, 2 months ago)

pour plates. it seems intimidating but it’s way easier than you think. this is coming from someone who just poured plates for the first time and 1 out of 20 got a contam.


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OfflineReliableSporesV
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: RogerTheRetard]
    #26341489 - 11/24/19 01:08 AM (4 years, 2 months ago)

Depends on what your goals are and what financial tolerance you have for failure.  A mix of glass and plastic is pretty essential for any fiscally responsible mycology lab.  But process is just as important.

Some petri dishes are for science and some are for propagation.

Glass is best for science and plastic is best for growing bulk agar spawn for transfers.

Either way, pouring your agar is by far the best practice for achieving maximum efficiency over time.

I hope that makes sense.

Pour that shit.


Edited by ReliableSpores (11/24/19 01:12 AM)


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OfflineGrowtech
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: ReliableSpores]
    #26341507 - 11/24/19 01:37 AM (4 years, 2 months ago)

Another vote to pour plates.  If you can pour milk into a bowl of cereal, you can do it.  It’s literally that easy.


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Mycolo.gyGenetics & yield tracking system


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OfflineTattersail
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: Growtech]
    #26342908 - 11/24/19 04:23 PM (4 years, 2 months ago)

No-pours are easy, and the high sides seem to help guard against contams during work, but I find it tedious wrapping them all in foil and unwrapping a bunch at a time. It's also harder to see through the lids, even if they are clear.

On the other hand, pour agar isn't difficult either. It was easier with the media bottle before I broke it, but I've been having success with pouring from a pint jar as well. The petris look pretty and Ive been experimenting with dish sizes.

Neither way is particularly costly, so maybe try both and see which you prefer.


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LAGM2021
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We may lose or we may win, but we'll never be here again


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InvisibleRogerTheRetard
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: Tattersail]
    #26343244 - 11/24/19 07:00 PM (4 years, 2 months ago)

Yeah, I'll try both methods. I'm sure I'll favor petris in the long run.

But I want to just try a mere 10 or so pp5 no pour plates to begin with.

My mind has been made up anyway, thank you community. :cheers:


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Offlinesan pedro guy
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: RogerTheRetard]
    #26343262 - 11/24/19 07:15 PM (4 years, 2 months ago)

I have about 6 or 7 pieces of glass for my no pours. I don’t get how people go thru hundreds?

I have a few growing out and 4 ready to go. I just don’t get it....


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Offlinecurious.psychonaut
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Re: Media bottle/petri dishes vs PP5 plastic no pour [Re: san pedro guy]
    #26343382 - 11/24/19 08:25 PM (4 years, 2 months ago)

Quote:

san pedro guy said:
I have about 6 or 7 pieces of glass for my no pours. I don’t get how people go thru hundreds?

I have a few growing out and 4 ready to go. I just don’t get it....




As a newb, it's also mindset/failure tolerance. I do not want to fail: it's expensive for me to obtain spores and cheap to get most of the other stuff needed for the hobby. (My single print cost me as much as my 500-pack of petris.) So I keep all my 16 T0 (spore) plates, some in the fridge, others pinning. I keep T1-T4 masters for all 29 jars I have currently running, so I can get back to any culture if shit hits the fan. Once I have fruits, I will clone the nicest & store them for future grows, also in petris.

I'd never do all this with no-pour, as my home would be overtaken by mini rounds and I'd go insane running PC-cycles for every handful of plates. With petris, it's an enjoyable part of the hobby, I find.


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My LAGM2020 grow log



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