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OfflineWeeds33d
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First attempt agar petris from syringes with pics, ready to transfer? Advice please
    #26340371 - 11/23/19 02:03 PM (4 years, 2 months ago)

Sorry typing from a phone so kind of shorthand. Anyways this is my first attempt innoculating agar petris. They were inoculated from syringes from ralphsters. Anyways it's been about2 to 3 weeks
Anyways how do they look I've looked at many pictures and they don't look contaminated from my judgement, what do u think. Are they ready to make the first transfer to other petri. Or should I let theses grow out more? So basically what's my next move? Thanks!







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OfflineMr_enforcer1
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Re: First attempt agar petris from syringes with pics, ready to transfer? Advice please [Re: Weeds33d]
    #26340417 - 11/23/19 02:28 PM (4 years, 2 months ago)

It looks like you used way too much, you only need a single drop out of the syringe. Inoculate in the middle and then try not to let the water roll around on the agar. You want to transfer before the mycelium reaches the edges this way you can see the best sectors


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HOW TO UPLOAD A PICTURE



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OfflineWeeds33d
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Re: First attempt agar petris from syringes with pics, ready to transfer? Advice please [Re: Mr_enforcer1]
    #26340459 - 11/23/19 02:45 PM (4 years, 2 months ago)

Yes I did use alot of solution,it kind of just shot out on accident. how did you tell. Anyways should I isolate the spider web looking sectors into other doshes


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OfflineDoyledozo
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Re: First attempt agar petris from syringes with pics, ready to transfer? Advice please [Re: Weeds33d]
    #26340550 - 11/23/19 03:36 PM (4 years, 2 months ago)

Im having the same luck with MS. Only luck ive had taking liquid to agar without making bacteria soup is the LC I made before I really started reading or bought agar. A tiny drop of that starts spreading within a cpl days. I managed to get to a 3rd transfer. I keep trying with my other syringes and keep coming up with pink slime. Ill have to take those to BRF and invitro a jar for each to get spores and clones. Pain in the ass but still fun and educational.


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Re: First attempt agar petris from syringes with pics, ready to transfer? Advice please [Re: Doyledozo]
    #26340564 - 11/23/19 03:42 PM (4 years, 2 months ago)

Quote:

Weeds33d said:
Yes I did use alot of solution,it kind of just shot out on accident. how did you tell. Anyways should I isolate the spider web looking sectors into other doshes



You can transfer from anywhere you want at this point in time.


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