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OfflineGreenbeast
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Small amount of Blue Oyster spawn
    #26340332 - 11/23/19 01:47 PM (4 years, 2 months ago)

Bought a 3ml spore syringe and inoculated 3x 1/2pint jars of wheat, unfortunately two of them seemed quite dry and developed contam.
So now i'm down to one jar which seems to be doing alright.

Trying to figure out what vessel to spawn them into given i'll not have a lot of material to work with!

Thoughts?


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OfflineHabadashery
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Re: Small amount of Blue Oyster spawn [Re: Greenbeast]
    #26340489 - 11/23/19 03:03 PM (4 years, 2 months ago)

Do you mean you bought a 3ml liquid culture? 

I would use that to make more grains.  Best bet for more mushrooms.


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OfflineSHROOMSISAY01M
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Re: Small amount of Blue Oyster spawn [Re: Habadashery]
    #26340495 - 11/23/19 03:06 PM (4 years, 2 months ago)

:whathesaid:

You can also take a small piece of mycelium from the jar and put it on agar. Then place the agar wedge once it is clean on grain. Then G2G your jars.

If you do not do something along these lines you will be starting over once you get the small amount from what you have. It is best to get ahead of the game now.


Edited by SHROOMSISAY01 (11/23/19 03:12 PM)


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OfflineGreenbeast
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Re: Small amount of Blue Oyster spawn [Re: Habadashery]
    #26340503 - 11/23/19 03:12 PM (4 years, 2 months ago)

Quote:

Habadashery said:
Do you mean you bought a 3ml liquid culture? 






Er, yes apparently! Apologies for the beginner question but what is the difference between a liquid culture and spore syringe (of which i've been buying for cube growing)


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OfflineSHROOMSISAY01M
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Re: Small amount of Blue Oyster spawn [Re: Greenbeast]
    #26340516 - 11/23/19 03:16 PM (4 years, 2 months ago)

A spore syringe once put on to spawn will need to germinate. This can take a long time. Liquid culture is mycelium that will colonize a jar quickly. We all had beginner questions so don't worry about it that is what this site is here for.

Either way, a spore syringe or a liquid culture syringe should be put to agar 1st. Then to grain. Then you can do G2G and keep everything going by doing G2G for a long time.


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OfflineGreenbeast
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Re: Small amount of Blue Oyster spawn [Re: SHROOMSISAY01]
    #26340528 - 11/23/19 03:25 PM (4 years, 2 months ago)

Many thanks, that explains why it's getting along so well compared to cube jars i inoculated 2 days prior!

Now reading about agar!


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OfflineSHROOMSISAY01M
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Re: Small amount of Blue Oyster spawn [Re: Greenbeast]
    #26340607 - 11/23/19 03:59 PM (4 years, 2 months ago)

Yes, read up on agar. Then I would suggest buying good spawn, from somewhere like Amycel, Aloha Medicinals, North Spore or Mycelia. Those are all good spawn providers for edibles. Where are you located I have some awesome edible strains to get you started without paying a lot for spawn.

Here is an example of the fruits I get with good spawn. Trust me genetics is everything when growing mushrooms...



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OfflineGreenbeast
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Re: Small amount of Blue Oyster spawn [Re: SHROOMSISAY01]
    #26341495 - 11/24/19 01:16 AM (4 years, 2 months ago)

I'm in the UK, but thanks for the offer :smile:

For this time i guess i will prep up a bunch more jars and distribute the mycelium between them, once it's consolidated


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OfflineFergent
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Re: Small amount of Blue Oyster spawn [Re: SHROOMSISAY01]
    #26342878 - 11/24/19 04:11 PM (4 years, 2 months ago)

Quote:

SHROOMSISAY01 said:
Here is an example of the fruits I get with good spawn. Trust me genetics is everything when growing mushrooms...






Genetics is indeed everything. pretty much every spawn producer in the western world is using the exact same genetics. especially when it comes to Lentinula and Pleurotus. It's only the asians (Japan, Korea and China) who are actively breeding new strains which are developed for their markets only. (An exception is Wageningen University which developed the Spoppo, or sporeless oyster.) it's only once in a while some European can visit their farms and snatch a mushroom from their blocks to move the genetics to the western market.


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OfflineHillbillyjohn
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Re: Small amount of Blue Oyster spawn [Re: Fergent]
    #26342889 - 11/24/19 04:17 PM (4 years, 2 months ago)

Just to toss it out there I wouldn't toss those bad jars oysters have been known to eat contams not always but I'd still watch it and see but at any rate you got good advice above I'm trying out blue oysters for the first time myself. So good luck and break a veil lol


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https://www.shroomery.org/forums/showflat.php/Number/24297804. Everything you need in one place read,follow,and fruit


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OfflineGreenbeast
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Re: Small amount of Blue Oyster spawn [Re: Hillbillyjohn]
    #26355703 - 12/01/19 02:35 PM (4 years, 1 month ago)

I transferred the single colonized jar to 8 more wheat jars and they are cracking on, hoping to spawn to bulk later this week.

Thanks all


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OfflineSHROOMSISAY01M
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Re: Small amount of Blue Oyster spawn [Re: Greenbeast]
    #26355754 - 12/01/19 03:12 PM (4 years, 1 month ago)

Make sure you save one jar to transfer again. You can easily make 15 jars from one jar just so you know it only takes a little.


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OfflineGreenbeast
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Re: Small amount of Blue Oyster spawn [Re: SHROOMSISAY01]
    #26357396 - 12/02/19 03:07 PM (4 years, 1 month ago)

Thanks, good idea. I've got 4x 1/2pint and 4x 1/4pint (all that will fit in my PC at once), so i'll save one of the 1/4 pints to spread again.


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