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OfflineTherealmcoy
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Question about agar
    #26339598 - 11/23/19 07:46 AM (4 years, 2 months ago)

So I inoculated agar with two different spore syringes from 2 different vendors there both golden teacher one looks good but one has massive amounts of tiny rust yellow dots popping up the other is white mycelium I think I got the wrong spores from one vendor does any mycelium grow rusty yellow


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OfflineSellith
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Re: Question about agar [Re: Therealmcoy]
    #26339602 - 11/23/19 07:48 AM (4 years, 2 months ago)

Might be a contam, not sure without seeing it honestly. - Could be a contam from when opening the lid on the dish, or maybe there was some contam in the syringe or on the needle.

Could be metabolites as well I suppose.


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InvisibleTeaforTwo
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Re: Question about agar [Re: Sellith]
    #26339610 - 11/23/19 07:55 AM (4 years, 2 months ago)

put up a picture


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OfflineTherealmcoy
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Re: Question about agar [Re: Sellith]
    #26339615 - 11/23/19 07:58 AM (4 years, 2 months ago)

I'll add a pic soon it's so tiny right now  I just cant get a good pic it doesnt look like any of the other mycelium can I take the mycelium of the agar and right into manure substrate and fruit and skip grain all together


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OfflineTherealmcoy
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Re: Question about agar [Re: Therealmcoy]
    #26339620 - 11/23/19 08:03 AM (4 years, 2 months ago)



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OfflineSellith
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Re: Question about agar [Re: Therealmcoy]
    #26339624 - 11/23/19 08:08 AM (4 years, 2 months ago)

Kinda looks like what happens if you squirt a large drop of spore syringe on agar and it pools the whole plate.

Could be something else like a contam too, would just wait a week or so, see what it looks like then.

Could also make a new plate from the same syringe.


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OfflineTherealmcoy
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Re: Question about agar [Re: Sellith]
    #26339627 - 11/23/19 08:10 AM (4 years, 2 months ago)

I made two with that syringe and two with the other


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InvisibleTeaforTwo
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Re: Question about agar [Re: Therealmcoy]
    #26339628 - 11/23/19 08:10 AM (4 years, 2 months ago)

when did you put spores on agar? how much did you squirt onto the agar? what was your agar prep recipe and what is the agar in now? (as in petris, glad minis, babyfood jars, etc)

help others to help you, tons of missing info wont solicit smart responses and no pics multiplies missing info a 1000 fold.

taking even a plate full of clean mycelium to bulk substrate sounds like a really bad idea that will likely only provide you contamination training.

clean up spores on agar by doing a transfer or three then take that resulting plate to grain and when grain is colonized either expand it to more grain and/or spawn to your bulk substrate of choice.


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InvisibleTeaforTwo
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Re: Question about agar [Re: TeaforTwo]
    #26339635 - 11/23/19 08:14 AM (4 years, 2 months ago)

seeing picture now, that needs to grow out before anything can be determined. 5-7 days from inoc, as long storage temps are not too low (below 65F), you'll see some pretty obvious growths.


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OfflinePsilo_citizen
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Re: Question about agar [Re: Therealmcoy]
    #26339638 - 11/23/19 08:16 AM (4 years, 2 months ago)

Kind of looks like wild yeast found its way into your plate.

You'll want to transfer healthy growth a few times before inoculating anything to ensure you have a clean culture.

Grain is for sure the better way to go.


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OfflineTherealmcoy
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Re: Question about agar [Re: TeaforTwo]
    #26339641 - 11/23/19 08:19 AM (4 years, 2 months ago)

I bought malt agar on amazon I squirted the least amount possible like 1 cc maybe 2 it's hard to tell it's been 3 days since I inoculated the agar and there in petri dishes


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OfflineTherealmcoy
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Re: Question about agar [Re: Therealmcoy]
    #26339643 - 11/23/19 08:20 AM (4 years, 2 months ago)

I'm only practicing with agar I have 4 rye bags and I can see tiny spots were they are growing mycelium


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OfflineTherealmcoy
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Re: Question about agar [Re: Therealmcoy]
    #26339646 - 11/23/19 08:22 AM (4 years, 2 months ago)

This is my first smart phone how in the hell do I upload pictures when I try to do it like I did the first one it won't let me


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OfflinePsilo_citizen
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Re: Question about agar [Re: Therealmcoy]
    #26339647 - 11/23/19 08:22 AM (4 years, 2 months ago)

Quote:

Therealmcoy said:
I bought malt agar on amazon I squirted the least amount possible like 1 cc maybe 2 it's hard to tell it's been 3 days since I inoculated the agar and there in petri dishes




A single drop is all you need or want. The more innoculant you use, the higher the chances are that you're bringing unwanted competition. It also makes it very difficult to spot the growth you're looking for.

If you're having issues getting a single drop out, consider building or buying an inoculation loop so you can streak a single drop.


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OfflineTherealmcoy
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Re: Question about agar [Re: Psilo_citizen]
    #26339650 - 11/23/19 08:26 AM (4 years, 2 months ago)

Can I use a qtip?


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InvisibleTeaforTwo
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Re: Question about agar [Re: Therealmcoy]
    #26339651 - 11/23/19 08:26 AM (4 years, 2 months ago)

1-2cc is way too much, one single drop will do and put right in the center of your plate. flame needle tip to glow red hot, squirt a couple drops onto papertowel to cool tip then squirt a single drop onto plate.

the more you squirt onto agar the more likely you will grow contaminants, the more contam the less likely you will be able to clean up by transfer away good clean myc to another plate for it to grow out.


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InvisibleTeaforTwo
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Re: Question about agar [Re: TeaforTwo]
    #26339652 - 11/23/19 08:28 AM (4 years, 2 months ago)

yes, you can use a sterile qtip to spread a fresh drop on agar. like using a loop to spread it. or you could just leave that single drop in the center.


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OfflineTherealmcoy
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Re: Question about agar [Re: Therealmcoy]
    #26339654 - 11/23/19 08:31 AM (4 years, 2 months ago)

Ya I've been really sterile I've got a glove box iso spray bottle and I use a torch for the needle tip


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InvisibleTeaforTwo
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Re: Question about agar [Re: Therealmcoy]
    #26339657 - 11/23/19 08:33 AM (4 years, 2 months ago)

glove box may cause you many contam issues down the road, consider build a Still Air Box (SAB) for your sterile work space.


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Be well, friend. Stay Strong and Beautiful, Always, and in All Ways~
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OfflineTherealmcoy
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Re: Question about agar [Re: TeaforTwo]
    #26339672 - 11/23/19 08:45 AM (4 years, 2 months ago)

O the temps perfect I have it at a constant 78 degrees using a seed mat


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