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OfflineRot
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Giving up on grains
    #26339566 - 11/23/19 07:18 AM (4 years, 2 months ago)

So this is pretty disheartening when I've spent months growing out my agar, waiting for the grain to colonize, never had a freaking contam EVER before getting a at least a first flush when using BRF to bulk.

My grain jars looked perfect, had no mold or anything, my agar was carefully grown out and now all of my tubs have mold. It is some kind of pin mold with little black heads, and it also has some form of green mold.

I'm not going to be doing grains again but does anyone know why these all contamed in the very last stage?

https://i.imgur.com/2BlSwjq.png


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Invisiblenatedawgnow
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Re: Giving up on grains [Re: Rot]
    #26339574 - 11/23/19 07:24 AM (4 years, 2 months ago)

What kind of grain are you using? What's your prep like? What are you using as
filter material? I wouldn't give up, I would troubleshoot the issue.

Most cultivators go through a contam phase. I had a solid year a few years back
where my success rate was 50% or less. It happens, dude. Best thing is to
change 1 or 2 variables at a time to narrow down the source. I'd start with filters.

Back when I had my issue I was using sfd siliconed onto metal lids. Terrible.
After getting fed up and finally switching my filters to tightly stuffed poly
covered with a round of ez felt, I haven't had any issues.


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InvisibleHobbit GDF
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Re: Giving up on grains [Re: Rot]
    #26339576 - 11/23/19 07:27 AM (4 years, 2 months ago)

Out of 12 tubs 10 got mold after first flush. One before it. I just had 10 fully colonized qt jars of oats have mold. I feel ya rot. This is with oats. Which I like better than brf and wbs. Good luck


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InvisibleSmartattack
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Re: Giving up on grains [Re: Hobbit GDF] * 2
    #26339596 - 11/23/19 07:44 AM (4 years, 2 months ago)

All that can be said is your problems are not BECAUSE of grains.


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OfflineRot
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Re: Giving up on grains [Re: Hobbit GDF]
    #26339600 - 11/23/19 07:47 AM (4 years, 2 months ago)

This was Rye berries. I am fully aware that contams happen and ways to prevent it from happening, but this is like $200+ spent and nearly 3 months of waiting to waste my damn time. I could of had 3-4 successful BRF grows in that time.

I was led to believe that grains were so much faster and amazing than brf. This has been nothing but a headache.

I was relying on taking a clone from this grow so now I have to start from square one and order more damn spores and spend 1-2 months growing it out on agar.


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Offlineinsutama
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Re: Giving up on grains [Re: Rot]
    #26339606 - 11/23/19 07:51 AM (4 years, 2 months ago)

Quote:

Rot said:
This was Rye berries. I am fully aware that contams happen and ways to prevent it from happening, but this is like $200+ spent and nearly 3 months of waiting to waste my damn time. I could of had 3-4 successful BRF grows in that time.

I was led to believe that grains were so much faster and amazing than brf. This has been nothing but a headache.

I was relying on taking a clone from this grow so now I have to start from square one and order more damn spores and spend 1-2 months growing it out on agar.




PM me and ill send you some spores buddy no need to spend any money


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Invisiblenatedawgnow
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Re: Giving up on grains [Re: Rot]
    #26339607 - 11/23/19 07:52 AM (4 years, 2 months ago)

Are you using agar?


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OfflineRot
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Re: Giving up on grains [Re: insutama]
    #26339617 - 11/23/19 07:58 AM (4 years, 2 months ago)

Quote:

natedawgnow said:
Are you using agar?




Yes. I did MS to agar, then did 2 transfers. Had beautiful rhizo growth.

I'm going to attempt to take a transfer from my old agar that has been sitting for a while.

Quote:

insutama said:
Quote:

Rot said:
This was Rye berries. I am fully aware that contams happen and ways to prevent it from happening, but this is like $200+ spent and nearly 3 months of waiting to waste my damn time. I could of had 3-4 successful BRF grows in that time.

I was led to believe that grains were so much faster and amazing than brf. This has been nothing but a headache.

I was relying on taking a clone from this grow so now I have to start from square one and order more damn spores and spend 1-2 months growing it out on agar.




PM me and ill send you some spores buddy no need to spend any money




I greatly appreciate that offer. Let me attempt to do a transfer today and if that fails I will definitely hit you up.


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InvisiblePsyduckMonkey
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Re: Giving up on grains [Re: Rot]
    #26339630 - 11/23/19 08:10 AM (4 years, 2 months ago)

Man your problems have nothing to do with grains, and everything to do with substrate. I suspect you partially sterilized the thing. :frown: Pasteurizing substrate properly is a bloody chore, but it's unskippable.

Several of the pasteurization teks here are of questionable repeatability, as it really depends on your specific stove, your specific ambient temperature, your specific kettle. You need to babysit it, with a thermometer in the water bath, and a thermometer in the middle of a substrate jar. All of the substrate need to be above 140F for at least an hour, and none of it can go above 170F for more than a few minutes. If it went above 170, it's garbage, nothing can be done to fix it.


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InvisibleSpunkyMonkey88
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Re: Giving up on grains [Re: PsyduckMonkey] * 1
    #26339637 - 11/23/19 08:15 AM (4 years, 2 months ago)

Quote:

PsyduckMonkey said:
Man your problems have nothing to do with grains, and everything to do with substrate. I suspect you partially sterilized the thing. :frown: Pasteurizing substrate properly is a bloody chore, but it's unskippable.

Several of the pasteurization teks here are of questionable repeatability, as it really depends on your specific stove, your specific ambient temperature, your specific kettle. You need to babysit it, with a thermometer in the water bath, and a thermometer in the middle of a substrate jar. All of the substrate need to be above 140F for at least an hour, and none of it can go above 170F for more than a few minutes. If it went above 170, it's garbage, nothing can be done to fix it.




Or you could just dump 3 qts of boiling water in a bucket with a brick of coir...


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InvisibleSpunkyMonkey88
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Re: Giving up on grains [Re: Rot]
    #26339639 - 11/23/19 08:17 AM (4 years, 2 months ago)

Quote:

Rot said:
So this is pretty disheartening when I've spent months growing out my agar, waiting for the grain to colonize, never had a freaking contam EVER before getting a at least a first flush when using BRF to bulk.

My grain jars looked perfect, had no mold or anything, my agar was carefully grown out and now all of my tubs have mold. It is some kind of pin mold with little black heads, and it also has some form of green mold.

I'm not going to be doing grains again but does anyone know why these all contamed in the very last stage?

https://i.imgur.com/2BlSwjq.png




Bro I'm interested in how many grams you get per cake spawned to bulk?


Edited by SpunkyMonkey88 (11/23/19 08:18 AM)


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OfflineDoyledozo
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Re: Giving up on grains [Re: PsyduckMonkey]
    #26339642 - 11/23/19 08:19 AM (4 years, 2 months ago)

If my shoeboxes go south ill be in the same boat. Having a very hard time putting MS syringes to agar. My expections have gotten so low i'll be happy to wind up with a print and a clone. Starting over from scratch would be very disheartening.


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OfflineSFS96
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Re: Giving up on grains [Re: SpunkyMonkey88] * 1
    #26339644 - 11/23/19 08:21 AM (4 years, 2 months ago)

:whathesaid: coir/verm doesn’t require pasteurization. Almost anyone who uses coir just bucket teks it but you don’t even have to do that. With coir you can literally just add water and use it. Pasteurization is required for shit and other substrates but not coir.


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InvisiblePsyduckMonkey
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Re: Giving up on grains [Re: Rot]
    #26339656 - 11/23/19 08:32 AM (4 years, 2 months ago)

Quote:

Rot said:
this is like $200+ spent and nearly 3 months of waiting to waste my damn time. I could of had 3-4 successful BRF grows in that time.

I was led to believe that grains were so much faster and amazing than brf. This has been nothing but a headache.



Sorry I'm quite lost here. What on Earth did you spend 3 months and 200 bucks on? You didn't have a pressure cooker and just bought one? A jar, when handled correctly, will colonize fully in a week from LC, and in two weeks from an agar wedge. Isolating strains on agar can be slower, but not 3 months...

The way your tub looks, you got a contam pretty much right after spawning... If the grains had a good smell, the fault is 100% in your substrate.

Quote:

SpunkyMonkey88 said:
Or you could just dump 3 qts of boiling water in a bucket with a brick of coir...



That only works with coir. :smile: Speaking of which, @Rot what was your substrate?


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OfflineRot
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Re: Giving up on grains [Re: PsyduckMonkey]
    #26339668 - 11/23/19 08:41 AM (4 years, 2 months ago)

Quote:

PsyduckMonkey said:
Quote:

Rot said:
this is like $200+ spent and nearly 3 months of waiting to waste my damn time. I could of had 3-4 successful BRF grows in that time.

I was led to believe that grains were so much faster and amazing than brf. This has been nothing but a headache.



Sorry I'm quite lost here. What on Earth did you spend 3 months and 200 bucks on? You didn't have a pressure cooker and just bought one? A jar, when handled correctly, will colonize fully in a week from LC, and in two weeks from an agar wedge. Isolating strains on agar can be slower, but not 3 months...

The way your tub looks, you got a contam pretty much right after spawning... If the grains had a good smell, the fault is 100% in your substrate.

Quote:

SpunkyMonkey88 said:
Or you could just dump 3 qts of boiling water in a bucket with a brick of coir...



That only works with coir. :smile: Speaking of which, @Rot what was your substrate?




Coir.


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InvisiblePsyduckMonkey
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Re: Giving up on grains [Re: Rot]
    #26339681 - 11/23/19 08:51 AM (4 years, 2 months ago)

Quote:

Rot said:
Quote:

PsyduckMonkey said:
That only works with coir. :smile: Speaking of which, @Rot what was your substrate?



Coir.



How did you prepare it?


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InvisibleSpunkyMonkey88
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Re: Giving up on grains [Re: PsyduckMonkey]
    #26339752 - 11/23/19 09:43 AM (4 years, 2 months ago)

Quote:

PsyduckMonkey said:
Quote:

Rot said:
Quote:

PsyduckMonkey said:
That only works with coir. :smile: Speaking of which, @Rot what was your substrate?



Coir.



How did you prepare it?




Dude I seriously doubt he contaminated his tubs with his coir...


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Re: Giving up on grains [Re: SpunkyMonkey88]
    #26339782 - 11/23/19 09:54 AM (4 years, 2 months ago)

...what are your temps?  I dunno... if yo're making it through colonization just fine and then growing mold, you might be having an issue with high temps and not enough fae... What was your ratio for that tub anyway?

Don't let me talk you out of it though:  I got a bunch of brown rice for free lately (a bunch...) and am on a wbr kick now and loving it.  Simple and quicker than wbs, rye, or popcorn IME.  What contams? :stoned:


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Edited by alaskappalachian (11/23/19 10:00 AM)


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InvisiblePsyduckMonkey
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Re: Giving up on grains [Re: SpunkyMonkey88]
    #26339788 - 11/23/19 09:56 AM (4 years, 2 months ago)

Quote:

SpunkyMonkey88 said:
Dude I seriously doubt he contaminated his tubs with his coir...



"Once you eliminate the impossible, whatever remains, no matter how improbable, must be the truth." - Sir Arthur Conan Doyle

The thing is, it couldn't have been the grains, as if the grains had been infected, the contam would have appeared in the jars. You hear about jars going bad all the time, while most growers agree that fully colonized grains are pretty much like a fortress of mycelium, you can wipe your ass with them and they will still be good.

If there were a lot of popped grains, it could have provided an easy attack surface after spawning, but the only thing that could have carried the contamination is the substrate.

(Another possibility is that the grains weren't fully colonized at spawn time... but let's not assume that, nobody does something that silly.)


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Offlinefootpath
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Re: Giving up on grains [Re: SpunkyMonkey88]
    #26339799 - 11/23/19 09:59 AM (4 years, 2 months ago)

Any pics of your agar dishes?
It's almost certainly stemming from that.
2 transfers isn't a solid guarantee from MS
Rhizo growth doesn't necessarily equate to clean growth.
I've had shit hide out in very uniform rhizo growth that didn't present itself until a fifth transfer.


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Invisiblealaskappalachian
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Re: Giving up on grains [Re: PsyduckMonkey]
    #26339809 - 11/23/19 10:01 AM (4 years, 2 months ago)

Quote:



(Another possibility is that the grains weren't fully colonized at spawn time... but let's not assume that, nobody does something that silly.)




Also this.


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Re: Giving up on grains [Re: footpath]
    #26339813 - 11/23/19 10:02 AM (4 years, 2 months ago)

Quote:

footpath said:
Any pics of your agar dishes?
It's almost certainly stemming from that.
2 transfers isn't a solid guarantee from MS
Rhizo growth doesn't necessarily equate to clean growth.
I've had shit hide out in very uniform rhizo growth that didn't present itself until a fifth transfer.



I seriously doubt that. You just got a contam at the fifth transfer. Sure, you'll have multiple strains that don't separate, but a mold won't hide in the mycelium. You'll see it right away, which is the whole point of agar.

I've never heard of mold hiding out in a jar of grain until spawning. A fully colonized jar that smells good is just plain beyond suspicion. (Or are you just trolling the poor guy, like the "rm -rf /" guys used to on Linux usenet groups?)


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Edited by PsyduckMonkey (11/23/19 10:03 AM)


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Offlinefootpath
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Re: Giving up on grains [Re: PsyduckMonkey]
    #26339858 - 11/23/19 10:18 AM (4 years, 2 months ago)

Maybe so.
I don't see it so farfetched that something could be inhibited by your predominate desired culture
and then take an opportunity to proliferate when the conditions are right for it to.
In fact, it seems quite normal.
Obviously though, yes, introduction is more common.
But when you're talking T2...?
It's absolutely feasible, especially to an untrained eye, that you're carrying more than just one thing.


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Re: Giving up on grains [Re: Rot]
    #26339872 - 11/23/19 10:24 AM (4 years, 2 months ago)

Quote:

Rot said:
Had beautiful rhizo growth.





lots of time peoples cultures may not be as clean as they think they are. got any pics of your cultures used for a2g?

:begger:


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OfflineRot
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Re: Giving up on grains [Re: mushboy]
    #26339910 - 11/23/19 10:50 AM (4 years, 2 months ago)

On a positive note at least I have something growing healthily...

https://i.imgur.com/M6qAS0n.png

https://i.imgur.com/9NvGn1L.png


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OfflineRot
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Re: Giving up on grains [Re: Rot]
    #26339917 - 11/23/19 10:53 AM (4 years, 2 months ago)

Here's the ones that were used for the A2G.

Edit: Also my friend made a suggestion that I hadn't thought of. Could it be my tap water causing the issue?



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Edited by Rot (11/23/19 11:01 AM)


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InvisiblemushboyMDiscord
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Re: Giving up on grains [Re: Rot] * 1
    #26339926 - 11/23/19 11:01 AM (4 years, 2 months ago)

Quote:

Rot said:





i dont like that culture. it has a star like pattern where the strands of myc seeem to grow around something..

or im nuts:kingtard:


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OfflineRot
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Re: Giving up on grains [Re: mushboy]
    #26339932 - 11/23/19 11:04 AM (4 years, 2 months ago)


Quote:

mushboy said:
Quote:

Rot said:





i dont like that culture. it has a star like pattern where the strands of myc seeem to grow around something..

or im nuts:kingtard:




I had a few that were similar and under my microscope I couldn't spot anything.


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InvisibleJ. Jack Flash
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Re: Giving up on grains [Re: PsyduckMonkey]
    #26339983 - 11/23/19 11:31 AM (4 years, 2 months ago)

Quote:

PsyduckMonkey said:
Quote:

footpath said:
Any pics of your agar dishes?
It's almost certainly stemming from that.
2 transfers isn't a solid guarantee from MS
Rhizo growth doesn't necessarily equate to clean growth.
I've had shit hide out in very uniform rhizo growth that didn't present itself until a fifth transfer.



I seriously doubt that. You just got a contam at the fifth transfer. Sure, you'll have multiple strains that don't separate, but a mold won't hide in the mycelium. You'll see it right away, which is the whole point of agar.

I've never heard of mold hiding out in a jar of grain until spawning. A fully colonized jar that smells good is just plain beyond suspicion. (Or are you just trolling the poor guy, like the "rm -rf /" guys used to on Linux usenet groups?)




yes this certainly happens.



10/10 failures. spawn looked off, but not moldy. all from the same project which never fruited. a concurrent project went 10/10 successes using the same techniques, same oats, same coir, same everything except culture. it started on the plates, couldn't see it. it traveled thru the jars, it fully colonized, wasn't moldy, and smelled like cubes. didn't look perfect, but wasn't obviously fucked, to me at least. then one by one, ten shoeboxes turned to shit.


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InvisiblemushboyMDiscord
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Re: Giving up on grains [Re: J. Jack Flash]
    #26339994 - 11/23/19 11:36 AM (4 years, 2 months ago)

Quote:

PsyduckMonkey said:

I've never heard of mold hiding out in a jar of grain until spawning.





thats how most people would describe bad spawn. all seems ok until spawning. starts to colonize and right before pins come in mold explodes.

damn near text book.


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Re: Giving up on grains [Re: mushboy]
    #26340012 - 11/23/19 11:44 AM (4 years, 2 months ago)

Quote:

mushboy said:
Quote:

Rot said:





i dont like that culture. it has a star like pattern where the strands of myc seeem to grow around something..

or im nuts:kingtard:




Can you edit that photo and circle what you're talking abkut? I'm new to agar and would like to avoid this kind of mess.

I never would of guessed that plate looks off and that scares me!


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Offlinefootpath
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Re: Giving up on grains [Re: SpunkyMonkey88]
    #26340018 - 11/23/19 11:47 AM (4 years, 2 months ago)

It's especially prevalent around 11 o'clock. See how it diverts and then rejoins, but still seems inhibited at the edge?


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InvisibleSpunkyMonkey88
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Re: Giving up on grains [Re: footpath]
    #26340028 - 11/23/19 11:52 AM (4 years, 2 months ago)

Quote:

footpath said:
It's especially prevalent around 11 o'clock. See how it diverts and then rejoins, but still seems inhibited at the edge?





Ok ya I see that! I thought maybe there were more spots though than that as he compared it to a star...

Man this has got me on edge! Lol I hope agar doesn't do me wrong!

I thought tams were viable on agar almost all the time. Didn't think you had to pay attention to how mycelium grows (around stuff)


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Re: Giving up on grains [Re: SpunkyMonkey88]
    #26340109 - 11/23/19 12:27 PM (4 years, 2 months ago)

Quote:

SpunkyMonkey88 said:
Quote:

footpath said:
It's especially prevalent around 11 o'clock. See how it diverts and then rejoins, but still seems inhibited at the edge?





Ok ya I see that! I thought maybe there were more spots though than that as he compared it to a star...

Man this has got me on edge! Lol I hope agar doesn't do me wrong!

I thought tams were viable on agar almost all the time. Didn't think you had to pay attention to how mycelium grows (around stuff)



You have to look out for mycelium trying to avoid something and the way it "reacts" to hidden contams. That's why you want even uniform growth.


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OfflinemeaculpaUIO
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Re: Giving up on grains [Re: gizmo1]
    #26340150 - 11/23/19 12:44 PM (4 years, 2 months ago)

You just have to use up all the mold spores eventually you will win


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OfflineFailboat
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Re: Giving up on grains [Re: gizmo1]
    #26340166 - 11/23/19 12:48 PM (4 years, 2 months ago)

What gizmo said, also iffy spawn is made much worse when mixed with overhydrated coir. Looking at your first pic, which ought to be uploaded on here, I would not think your coir was overhydrated if even hydrated enough, but the fact that there's a whole mess of uncolonized looking grains throughout the sub screams bad spawn.


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Re: Giving up on grains [Re: meaculpaUIO]
    #26340188 - 11/23/19 12:52 PM (4 years, 2 months ago)

Start a grow log and document everything, often times somebody else's eyes will spot shitty spawn.


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Re: Giving up on grains [Re: cronicr]
    #26340205 - 11/23/19 12:59 PM (4 years, 2 months ago)

Can you use a small pin to colonize another jar


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Re: Giving up on grains [Re: meaculpaUIO]
    #26340214 - 11/23/19 01:02 PM (4 years, 2 months ago)

Not reliably unless it's an agar pin


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It doesn't matter what i think of you...all that matters is clean spawn

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Offlineverum subsequentis
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Re: Giving up on grains [Re: PsyduckMonkey] * 1
    #26340243 - 11/23/19 01:13 PM (4 years, 2 months ago)

Quote:

PsyduckMonkey said:
I've never heard of mold hiding out in a jar of grain until spawning. A fully colonized jar that smells good is just plain beyond suspicion. (Or are you just trolling the poor guy




Stick around, you'll hear of it. Actually, i digress. Congratulations, you just heard of it.

Footpath is the farthest thing from a troll you'll ever encounter. That's like asking anton lavey if he's a christian.

@rot, I'd strongly advise not giving up on grain. Grain is not your problem. Dirty cultures, improper sterile tek, improper hydration/ sterilization, air filtration of grain.... Any of these can cause you too waste a lot of time. It's only truly  waste, though, if you fail to learn and grow. If you think about it long and hard you'll find the flaw in your former tek and be better for it.

There is nothing wrong with doing up a bunch of cakes and going on a clone hunt. But, if you really want to make some magic, you'll want to be working with grain spawn.

Also, you should never have to buy more spores. If you don't have spores or clean cultures in cold storage, you should transfer until clean and then make up a master plate or slant and throw it in cold storage. That way, if you screw the pouch, you have a back up and cn quickly and easily try again.


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Re: Giving up on grains [Re: insutama]
    #26341284 - 11/23/19 09:28 PM (4 years, 2 months ago)

Bless you....and thank you for existing


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Re: Giving up on grains [Re: Whanjohi]
    #26341860 - 11/24/19 07:43 AM (4 years, 2 months ago)

Thanks for the enlightening info. So far I've always went by the adage that if something's wrong, you can smell it, good to hear contrary opinions from people who cultivated a lot more than I did.

Is there a tek you can recommend on reliably cleaning up a culture from spores on agar? Most of the stuff I've heard so far concentrates on getting even growth - that said, I've had transfers where an even plate transferred developed two separate sectors, so there might be more to it... How many transfers does it usually take you to get a reliable single strain?


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Do you believe in the Third Summer of Love?


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Re: Giving up on grains [Re: PsyduckMonkey]
    #26341869 - 11/24/19 07:50 AM (4 years, 2 months ago)



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OnlineLogicaL ChaosM
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Re: Giving up on grains [Re: Rot]
    #26341875 - 11/24/19 07:59 AM (4 years, 2 months ago)

Looks like cobweb mold.

I wouldnt give up on grains. However i recommend scaling down with grains to shoebox size or the Pod Tek so u can reduce your risk of failure such as seen here.

Hopefully u still have some agar plates going.....


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"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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Offlinefootpath
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Re: Giving up on grains [Re: PsyduckMonkey]
    #26341937 - 11/24/19 08:46 AM (4 years, 2 months ago)

Quote:

PsyduckMonkey said:
Is there a tek you can recommend on reliably cleaning up a culture from spores on agar?




The tek is basically you - your trained eye, your steady, careful hands.
Stro shows clearly that he has a trained eye and tuned hands.
Of course you could use sophisticated instruments and like to further guarantee that your eye is seeing things right, but even those aren't totally guaranteed.

You're right to take my words with skepticism, as I'm newly registered and haven't shared anything of my exploits... yet.
So, maybe someone notably seasoned has better insights or an actual 'Tek' for you to follow that I'm unaware of.


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Re: Giving up on grains [Re: footpath]
    #26342829 - 11/24/19 03:50 PM (4 years, 2 months ago)

Keep going with BRF jars while you are working on getting grains right. At least you'll have a good chance of a harvest and won't feel so much pressure if the grain jars fail. I still haven't had a successful grow from grains (its been a lot of trial and error with agar) but my BRF jars keep me from losing all hope!


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LAGM2021
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Re: Giving up on grains [Re: Tattersail]
    #26343051 - 11/24/19 05:16 PM (4 years, 2 months ago)

Hey guys. Since we're taking about things hiding out in grains until spawning you should check out this crazy Contam. I thought I had germinated pan cyan on accident. I grew it out several transfers, a2g colonized a quart jar, even put it side by side with real pans. Only when it started running out of nutes did it finally sporelate. I could totally see whatever this is piggybacking myc from plate to plate. In my case this contam was the only thing to germinate so I grew it out to see what it was.

Here you can see it currently still hasn't produced spores and as it grew into the pans (left) it merged with no sign of conflict. I don't know what this contam is but it's sneaky.


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Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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Re: Giving up on grains [Re: spiritlands]
    #26343087 - 11/24/19 05:33 PM (4 years, 2 months ago)

what color was the sporulation.


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Re: Giving up on grains [Re: Rot]
    #26343091 - 11/24/19 05:39 PM (4 years, 2 months ago)

The contam could have also slipped in during the a2g session if not done properly.  That spot on that plate did look strange tho...  my first attempt at grain failed too.  If first you dont succeed tho try try again


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


Edited by Psicomb (11/24/19 05:40 PM)


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Re: Giving up on grains [Re: mushboy]
    #26343412 - 11/24/19 08:42 PM (4 years, 2 months ago)

Quote:

mushboy said:
what color was the sporulation.



It's in the link at the end but I'll show it here too. It looked like black dots like pinhead mold but the structures are along the surface like a pumpkin patch with Mycelium sticking up overhead. They first appeared in the thin agar ring I left at the edge of the plate. The grain and this pitri have never shown spores yet but once the other plate began it continues to spread even where there were still nutes.

First pic shows the contam spore structures. You can tell they're on the surface by looking at the edges where I cut.

Second pic is pan cyan (left) contam t1 (right)

Third pic is contam original plate just before a2g.


I shook that jar with the clump in it and it colonized in 5 days. When I saw the spores on agar I put the jar in the PC just before it consolidated to kill it before dumping it out.


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




Edited by spiritlands (11/24/19 08:52 PM)


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