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OfflineWhanjohi
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Registered: 09/24/19
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Re: Giving up on grains [Re: insutama]
    #26341284 - 11/23/19 09:28 PM (4 years, 2 months ago)

Bless you....and thank you for existing


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InvisiblePsyduckMonkey
witch
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Registered: 10/12/18
Posts: 273
Re: Giving up on grains [Re: Whanjohi]
    #26341860 - 11/24/19 07:43 AM (4 years, 2 months ago)

Thanks for the enlightening info. So far I've always went by the adage that if something's wrong, you can smell it, good to hear contrary opinions from people who cultivated a lot more than I did.

Is there a tek you can recommend on reliably cleaning up a culture from spores on agar? Most of the stuff I've heard so far concentrates on getting even growth - that said, I've had transfers where an even plate transferred developed two separate sectors, so there might be more to it... How many transfers does it usually take you to get a reliable single strain?


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Do you believe in the Third Summer of Love?


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OfflineDoyledozo
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Registered: 10/22/19
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Re: Giving up on grains [Re: PsyduckMonkey]
    #26341869 - 11/24/19 07:50 AM (4 years, 2 months ago)



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OfflineLogicaL ChaosM
Ascension Energy & Alien UFOs
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Re: Giving up on grains [Re: Rot]
    #26341875 - 11/24/19 07:59 AM (4 years, 2 months ago)

Looks like cobweb mold.

I wouldnt give up on grains. However i recommend scaling down with grains to shoebox size or the Pod Tek so u can reduce your risk of failure such as seen here.

Hopefully u still have some agar plates going.....


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"What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin

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Offlinefootpath
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Registered: 07/16/19
Posts: 1,367
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Re: Giving up on grains [Re: PsyduckMonkey]
    #26341937 - 11/24/19 08:46 AM (4 years, 2 months ago)

Quote:

PsyduckMonkey said:
Is there a tek you can recommend on reliably cleaning up a culture from spores on agar?




The tek is basically you - your trained eye, your steady, careful hands.
Stro shows clearly that he has a trained eye and tuned hands.
Of course you could use sophisticated instruments and like to further guarantee that your eye is seeing things right, but even those aren't totally guaranteed.

You're right to take my words with skepticism, as I'm newly registered and haven't shared anything of my exploits... yet.
So, maybe someone notably seasoned has better insights or an actual 'Tek' for you to follow that I'm unaware of.


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OfflineTattersail
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Registered: 04/11/18
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Re: Giving up on grains [Re: footpath]
    #26342829 - 11/24/19 03:50 PM (4 years, 2 months ago)

Keep going with BRF jars while you are working on getting grains right. At least you'll have a good chance of a harvest and won't feel so much pressure if the grain jars fail. I still haven't had a successful grow from grains (its been a lot of trial and error with agar) but my BRF jars keep me from losing all hope!


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LAGM2021
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We may lose or we may win, but we'll never be here again


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Offlinespiritlands
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Re: Giving up on grains [Re: Tattersail]
    #26343051 - 11/24/19 05:16 PM (4 years, 2 months ago)

Hey guys. Since we're taking about things hiding out in grains until spawning you should check out this crazy Contam. I thought I had germinated pan cyan on accident. I grew it out several transfers, a2g colonized a quart jar, even put it side by side with real pans. Only when it started running out of nutes did it finally sporelate. I could totally see whatever this is piggybacking myc from plate to plate. In my case this contam was the only thing to germinate so I grew it out to see what it was.

Here you can see it currently still hasn't produced spores and as it grew into the pans (left) it merged with no sign of conflict. I don't know what this contam is but it's sneaky.


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Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




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InvisiblemushboyMDiscord
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Registered: 04/24/05
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Re: Giving up on grains [Re: spiritlands]
    #26343087 - 11/24/19 05:33 PM (4 years, 2 months ago)

what color was the sporulation.


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InvisiblePsicomb
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Registered: 01/13/18
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Re: Giving up on grains [Re: Rot]
    #26343091 - 11/24/19 05:39 PM (4 years, 2 months ago)

The contam could have also slipped in during the a2g session if not done properly.  That spot on that plate did look strange tho...  my first attempt at grain failed too.  If first you dont succeed tho try try again


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When we constantly pull things apart trying to see how it works, we may end up with only an understanding of how to destroy something
- nick sand


Edited by Psicomb (11/24/19 05:40 PM)


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Offlinespiritlands
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Re: Giving up on grains [Re: mushboy]
    #26343412 - 11/24/19 08:42 PM (4 years, 2 months ago)

Quote:

mushboy said:
what color was the sporulation.



It's in the link at the end but I'll show it here too. It looked like black dots like pinhead mold but the structures are along the surface like a pumpkin patch with Mycelium sticking up overhead. They first appeared in the thin agar ring I left at the edge of the plate. The grain and this pitri have never shown spores yet but once the other plate began it continues to spread even where there were still nutes.

First pic shows the contam spore structures. You can tell they're on the surface by looking at the edges where I cut.

Second pic is pan cyan (left) contam t1 (right)

Third pic is contam original plate just before a2g.


I shook that jar with the clump in it and it colonized in 5 days. When I saw the spores on agar I put the jar in the PC just before it consolidated to kill it before dumping it out.


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Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:




Edited by spiritlands (11/24/19 08:52 PM)


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