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alaskappalachian
Entitiologist


Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
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Quote:
(Another possibility is that the grains weren't fully colonized at spawn time... but let's not assume that, nobody does something that silly.)
Also this.
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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PsyduckMonkey
witch



Registered: 10/12/18
Posts: 273
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Re: Giving up on grains [Re: footpath]
#26339813 - 11/23/19 10:02 AM (4 years, 2 months ago) |
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Quote:
footpath said: Any pics of your agar dishes? It's almost certainly stemming from that. 2 transfers isn't a solid guarantee from MS Rhizo growth doesn't necessarily equate to clean growth. I've had shit hide out in very uniform rhizo growth that didn't present itself until a fifth transfer.
I seriously doubt that. You just got a contam at the fifth transfer. Sure, you'll have multiple strains that don't separate, but a mold won't hide in the mycelium. You'll see it right away, which is the whole point of agar.
I've never heard of mold hiding out in a jar of grain until spawning. A fully colonized jar that smells good is just plain beyond suspicion. (Or are you just trolling the poor guy, like the "rm -rf /" guys used to on Linux usenet groups?)
-------------------- Do you believe in the Third Summer of Love?
Edited by PsyduckMonkey (11/23/19 10:03 AM)
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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Maybe so. I don't see it so farfetched that something could be inhibited by your predominate desired culture and then take an opportunity to proliferate when the conditions are right for it to. In fact, it seems quite normal. Obviously though, yes, introduction is more common. But when you're talking T2...? It's absolutely feasible, especially to an untrained eye, that you're carrying more than just one thing.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,273
Loc: where?
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Re: Giving up on grains [Re: Rot]
#26339872 - 11/23/19 10:24 AM (4 years, 2 months ago) |
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Quote:
Rot said: Had beautiful rhizo growth.
lots of time peoples cultures may not be as clean as they think they are. got any pics of your cultures used for a2g?
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Rot
Worlds best trichoderma farmer


Registered: 11/09/18
Posts: 908
Last seen: 2 years, 8 months
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Re: Giving up on grains [Re: mushboy]
#26339910 - 11/23/19 10:50 AM (4 years, 2 months ago) |
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On a positive note at least I have something growing healthily...
https://i.imgur.com/M6qAS0n.png
https://i.imgur.com/9NvGn1L.png
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Rot
Worlds best trichoderma farmer


Registered: 11/09/18
Posts: 908
Last seen: 2 years, 8 months
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Re: Giving up on grains [Re: Rot]
#26339917 - 11/23/19 10:53 AM (4 years, 2 months ago) |
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Here's the ones that were used for the A2G.
Edit: Also my friend made a suggestion that I hadn't thought of. Could it be my tap water causing the issue?
Edited by Rot (11/23/19 11:01 AM)
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mushboy
modboy



Registered: 04/24/05
Posts: 32,273
Loc: where?
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Re: Giving up on grains [Re: Rot] 1
#26339926 - 11/23/19 11:01 AM (4 years, 2 months ago) |
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Quote:
Rot said:

i dont like that culture. it has a star like pattern where the strands of myc seeem to grow around something..
or im nuts
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Rot
Worlds best trichoderma farmer


Registered: 11/09/18
Posts: 908
Last seen: 2 years, 8 months
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Re: Giving up on grains [Re: mushboy]
#26339932 - 11/23/19 11:04 AM (4 years, 2 months ago) |
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Quote:
mushboy said:
Quote:
Rot said:

i dont like that culture. it has a star like pattern where the strands of myc seeem to grow around something..
or im nuts
I had a few that were similar and under my microscope I couldn't spot anything.
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J. Jack Flash
stranger than ever.

Registered: 11/20/13
Posts: 1,500
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Quote:
PsyduckMonkey said:
Quote:
footpath said: Any pics of your agar dishes? It's almost certainly stemming from that. 2 transfers isn't a solid guarantee from MS Rhizo growth doesn't necessarily equate to clean growth. I've had shit hide out in very uniform rhizo growth that didn't present itself until a fifth transfer.
I seriously doubt that. You just got a contam at the fifth transfer. Sure, you'll have multiple strains that don't separate, but a mold won't hide in the mycelium. You'll see it right away, which is the whole point of agar.
I've never heard of mold hiding out in a jar of grain until spawning. A fully colonized jar that smells good is just plain beyond suspicion. (Or are you just trolling the poor guy, like the "rm -rf /" guys used to on Linux usenet groups?)
yes this certainly happens.

10/10 failures. spawn looked off, but not moldy. all from the same project which never fruited. a concurrent project went 10/10 successes using the same techniques, same oats, same coir, same everything except culture. it started on the plates, couldn't see it. it traveled thru the jars, it fully colonized, wasn't moldy, and smelled like cubes. didn't look perfect, but wasn't obviously fucked, to me at least. then one by one, ten shoeboxes turned to shit.
--------------------
the j stands for jesus.2020 new years grow along
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mushboy
modboy



Registered: 04/24/05
Posts: 32,273
Loc: where?
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Quote:
PsyduckMonkey said:
I've never heard of mold hiding out in a jar of grain until spawning.
thats how most people would describe bad spawn. all seems ok until spawning. starts to colonize and right before pins come in mold explodes.
damn near text book.
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: Giving up on grains [Re: mushboy]
#26340012 - 11/23/19 11:44 AM (4 years, 2 months ago) |
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Quote:
mushboy said:
Quote:
Rot said:

i dont like that culture. it has a star like pattern where the strands of myc seeem to grow around something..
or im nuts
Can you edit that photo and circle what you're talking abkut? I'm new to agar and would like to avoid this kind of mess.
I never would of guessed that plate looks off and that scares me!
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footpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
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It's especially prevalent around 11 o'clock. See how it diverts and then rejoins, but still seems inhibited at the edge?
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Re: Giving up on grains [Re: footpath]
#26340028 - 11/23/19 11:52 AM (4 years, 2 months ago) |
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Quote:
footpath said: It's especially prevalent around 11 o'clock. See how it diverts and then rejoins, but still seems inhibited at the edge?
Ok ya I see that! I thought maybe there were more spots though than that as he compared it to a star...
Man this has got me on edge! Lol I hope agar doesn't do me wrong!
I thought tams were viable on agar almost all the time. Didn't think you had to pay attention to how mycelium grows (around stuff)
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gizmo1



Registered: 06/15/11
Posts: 3,831
Loc: FREEDOM
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Quote:
SpunkyMonkey88 said:
Quote:
footpath said: It's especially prevalent around 11 o'clock. See how it diverts and then rejoins, but still seems inhibited at the edge?
Ok ya I see that! I thought maybe there were more spots though than that as he compared it to a star...
Man this has got me on edge! Lol I hope agar doesn't do me wrong!
I thought tams were viable on agar almost all the time. Didn't think you had to pay attention to how mycelium grows (around stuff)
You have to look out for mycelium trying to avoid something and the way it "reacts" to hidden contams. That's why you want even uniform growth.
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meaculpaUIO



Registered: 08/26/19
Posts: 956
Last seen: 4 years, 1 month
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Re: Giving up on grains [Re: gizmo1]
#26340150 - 11/23/19 12:44 PM (4 years, 2 months ago) |
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You just have to use up all the mold spores eventually you will win
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 2 days, 20 hours
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Re: Giving up on grains [Re: gizmo1]
#26340166 - 11/23/19 12:48 PM (4 years, 2 months ago) |
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What gizmo said, also iffy spawn is made much worse when mixed with overhydrated coir. Looking at your first pic, which ought to be uploaded on here, I would not think your coir was overhydrated if even hydrated enough, but the fact that there's a whole mess of uncolonized looking grains throughout the sub screams bad spawn.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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Start a grow log and document everything, often times somebody else's eyes will spot shitty spawn.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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meaculpaUIO



Registered: 08/26/19
Posts: 956
Last seen: 4 years, 1 month
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Re: Giving up on grains [Re: cronicr]
#26340205 - 11/23/19 12:59 PM (4 years, 2 months ago) |
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Can you use a small pin to colonize another jar
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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Not reliably unless it's an agar pin
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
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Quote:
PsyduckMonkey said: I've never heard of mold hiding out in a jar of grain until spawning. A fully colonized jar that smells good is just plain beyond suspicion. (Or are you just trolling the poor guy
Stick around, you'll hear of it. Actually, i digress. Congratulations, you just heard of it.
Footpath is the farthest thing from a troll you'll ever encounter. That's like asking anton lavey if he's a christian.
@rot, I'd strongly advise not giving up on grain. Grain is not your problem. Dirty cultures, improper sterile tek, improper hydration/ sterilization, air filtration of grain.... Any of these can cause you too waste a lot of time. It's only truly waste, though, if you fail to learn and grow. If you think about it long and hard you'll find the flaw in your former tek and be better for it.
There is nothing wrong with doing up a bunch of cakes and going on a clone hunt. But, if you really want to make some magic, you'll want to be working with grain spawn.
Also, you should never have to buy more spores. If you don't have spores or clean cultures in cold storage, you should transfer until clean and then make up a master plate or slant and throw it in cold storage. That way, if you screw the pouch, you have a back up and cn quickly and easily try again.
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