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pakarang
Stranger
Registered: 11/02/19
Posts: 6
Last seen: 3 years, 10 months
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Game over? First grow, pink contamination
#26339550 - 11/23/19 06:40 AM (4 years, 2 months ago) |
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First grow, 12 BRF jars. Spore syringe from Zamnesia. Steamed the jars 90 mins, then inoculated. Swabbed and flamed the needle between each jar.
Growth has been white from what I could see up to today, day 6. Now ALL 12 look like below. Is this game over? What did I do wrong to get this in all 12?!
Any help much appreciated!

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SFS96
AstroMan



Registered: 12/09/18
Posts: 2,144
Loc: Valleys Of Neptune
Last seen: 2 days, 6 hours
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Re: Game over? First grow, pink contamination [Re: pakarang]
#26339634 - 11/23/19 08:14 AM (4 years, 2 months ago) |
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Those are definitely trash, looks like straight mold. It could possibly be a dirty syringe if you did everything else right.
-------------------- How I make and preserve tea
Consuming consumes a man That was never a purpose of life To only crave for material joys Is believing the lie - Mellow Mood
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Vinci
I'm You



Registered: 08/26/19
Posts: 629
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Re: Game over? First grow, pink contamination [Re: SFS96]
#26339817 - 11/23/19 10:05 AM (4 years, 2 months ago) |
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I don't see discoloration, sometimes myc grows strange from spores, I'd wait it out. If it turns colors or smells bad at birth toss em
-------------------- Vinci loves you
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pakarang
Stranger
Registered: 11/02/19
Posts: 6
Last seen: 3 years, 10 months
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Re: Game over? First grow, pink contamination [Re: Vinci]
#26340171 - 11/23/19 12:49 PM (4 years, 2 months ago) |
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here's a pic with flash so you can see the discolouration more clearly - what do you think?
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Failboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
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Re: Game over? First grow, pink contamination [Re: pakarang]
#26340193 - 11/23/19 12:54 PM (4 years, 2 months ago) |
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Maybe the camera is playing tricks on me but I swear you already got green.
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FreeCanadianHugs
Stranger

Registered: 08/07/19
Posts: 67
Last seen: 1 year, 10 months
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Re: Game over? First grow, pink contamination [Re: pakarang]
#26340221 - 11/23/19 01:04 PM (4 years, 2 months ago) |
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My guess is that steaming was not sufficient to sterilize your brf. I've read a lot of posts from people asking about why they had contamination after not using a pressure cooker. If you were using a pressure cooker at 15 PSI or more for 90 mins or more then it's likely your syringes were contaminated.
The way I look at it is there are a lot of microorganisms that would like to eat your brown rice flour as it's nutritious and at field capacity has enough water to support growth. We sterilize to kill everything to give our spores the best chance to colonize free of any competitors. Once the spores have germinated and colonized fully the mycellium is able to fight off competitors more or less while it has sufficient nutrients and water. But some microorganisms colonize faster than our fungi. Properly sterilized it's always the way to start to give you the best chance for success. Sometimes spore syringes will have contamination which is why a lot of people like to start off with agar to ensure a clean start and then do transfers of colonized agar into a sterilized medium like grains. Maybe you can sterilize without a pressure cooker and use a stock pot to steam sterilize but that's a big maybe and if that's how you sterilized then that's the most likely reason why it failed.
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