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Offlinecurious.psychonaut
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I'm a teapot User Gallery


Registered: 10/17/19
Posts: 282
Last seen: 4 years, 10 days
Re: Avoiding buying anymore spores [Re: Kazoo_bard]
    #26340348 - 11/23/19 01:53 PM (4 years, 2 months ago)

Quote:

Kazoo_bard said:
Gotcha, plus with careful cloning and management of your "trees" of growth.  You should be able to make A LOT it would seem.  Starting at a spore string, make 10 agar plates, make one into a massive jar of lc, freeze 8, use the other to make 10 more agar plates, inoculate 20 or 30 grain jars with that. Make jars out the ass with the liquid culture, and you still have 8 first or second gen plates depending on how long it took to clean it down.  A bucket of early gen lc and then theres the aspect of taking clones to make another set of plates and more LC. Assuming you keep track of everything. It seems like you could conceivably take a single syringe and stretch it to literally thousands of grain jars within the first 5 or 6 generations, and have gallons of culture on hand. Not to mention tons of preserved early plates to keep stretching g from.  But EVENTUALLY you would need to take your own spore print.  But by that time you should be pretty good with sterile work and agar that it should be a breeze.
This is just my take on it and piecing together what I've seen on different posts on here.  Does that sound about right?




Eventually, yes, but only because cultures do not last forever (though they can last for many years in a slant at 2C/36F). You can expand a fresh clone without practical limits and without senescence being an issue at all. After a couple of transfers, you can have millions of petris/slants to take over the world (galaxy, etc.), keeping them in the fridge until needed.


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My LAGM2020 grow log



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Offlinefootpath
ὕδωρχοίρος

Registered: 07/16/19
Posts: 1,367
Last seen: 1 year, 2 months
Re: Avoiding buying anymore spores [Re: Kazoo_bard]
    #26340356 - 11/23/19 01:58 PM (4 years, 2 months ago)

Yep.
Basically though, as grim said, I don't think you'll ever be working with a single culture long enough to not get bored/intrigued to take some spores and go onto a new, fresh set of traits.

Having those master cultures refrigerated (or dried...I think?) gives you the ability to always go back to a culture if you so decide. Again, to an extent. Many variables at play. That's down the road for most cultivators.


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OfflineKazoo_bard
Kid Blunder

Registered: 09/27/19
Posts: 138
Last seen: 3 years, 7 months
Re: Avoiding buying anymore spores [Re: footpath]
    #26340697 - 11/23/19 04:43 PM (4 years, 2 months ago)

I'm sure you're right. But it's interesting to learn about how far they can really go.  Even if it's not practical, knowledge for the sake of knowledge is cool. Hence my 10,000 stupid questions I post on here haha


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