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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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EAT LIQUID INOCULATES™ 5
#26337900 - 11/22/19 10:31 AM (4 years, 2 months ago) |
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EAT LIQUID INOCULATES™  A Step By Step Guide

Brought to you by THE TRIBE
Hello Shroomery!

This is how I liquid inoculate my quart grain jars. This tek is also assuming you know your SAB , Shmuvbox or Flowhood technique. In principle you can use any of these but the movements and open air exposure may vary. We will illustrate how it is done with a SAB. Some of the images show a Schmuvbox just for illustration purposes. We are focusing on the technique of pouring the liquid inoculant into receiving quart jars easily and evenly with a custom pour lid. You may use a flow hood or SAB for the method just make sure you stick to the principles of the method you prefer. You can also do blenderless liquid inoculate but I like using a blender and a nice pour lid. The distribution is near perfect with a flick of the wrist and the jars generally colonize in 7-10 days without having to shake afte your initial inoculation and shake. This recipe is 1 plate to 10 quart grain jars. You have the potential to stretch this to 20 quarts per plate but your colonization time may be slower than what is shown. Supplies for blender attachment and pour lid build:high temperature RTV silicone in a can

half pint tall regular mouth mason jar with metal band

half pint regular mouth mason jar pour lid that fits a straw

plug for pour lid. I use the blue injection ports shown below as a plug to close the pour lid.

Oster Brand blender blade assembly and grey gasket
http://www.goodmans.net/i/2589/ice-crusher-blender-blade-cutter-for-oster-osteriz.htm
medium grade aluminum foil

OPTIONAL POUR LID IF YOU DONT WANNA BUILD ONE
1) This is autocleaveable but you don’t want to go past 15 psi for 30 minutes. I find this to be the best pour lid hands down. It snaps closed between pours and gives a nice even pour.

https://www.masontops.com/collections/tough-tops/products/multi-tops-easy-sip-pour-mason-jar-lids
2) This is available on amazon. It is stainless steel and fits regular mouth mason jars. It is intended for use as an oil and vinegar type pour for viscus liquids.
   
https://www.amazon.com/Blulu-Pieces-Spout-Dispenser-Compatible/dp/B07YDQPVKR/ref=pd_aw_sbs_79_1/144-0245057-7019743?_encoding=UTF8&pd_rd_i=B07YDQPVKR&pd_rd_r=7aa1a725-3c8a-4319-ad72-2e893ec39caa&pd_rd_w=TeXbT&pd_rd_wg=x6abl&pf_rd_p=5c6e5bde-0dc6-44bf-853b-26c318f4dfa4&pf_rd_r=P2P0JWR6EAY8ZJKCC6JN&psc=1&refRID=P2P0JWR6EAY8ZJKCC6JN
We tested the pour lid two ways with 1/4 pint of water in a regular mouth half pint.
First one is the normal setting where the spout comes down about 1/4 inch below the bottom of the lid. This is how the lid is intended for use with oil. I’m assuming you want a delay with oil so a large amount doesnt come gushing all over the place. I also can’t get the remainder of the liquid out at the bottom of the batch and I feel it’s wasteful.


The 2nd way we put the spout flush with the bottom of the lid. This seems to pour a little better and more controlled. I’m not sure I like the cap it’s a little funky. But it does provide a nice cover between pours if you need to take a break and it’s automatic. You could use your gloved finger to push it closed as well. I want a quick motion where I flick once and it lets out as much inoculant as I need per jar. So what I’ve done in the image below is sit the spout flush with the bottom of the lid.

You can see how with the spout flush, the water comes out in one quick spurt. This is how it works with the flush pour lid. This is ideal.

SUPPLIES FOR INOCULATION
- mostly colonized pasty plate of your favorite or best clean culture that is not touching the edge of the plate.

- 70% isopropyl alcohol

- spray bottle with a fine mist

- SAB, Flowhood, Shmuvbox
  
- Prepared grain jars of your choice. Oats are shown in this image

- oster blender base
 This one is the cheapest on this site but any will work.
http://www.goodmans.net/i/4697/oster-6629-bk0-10-speed-slope-blender-black.htm

Procedure for building the pour lid
1) take the band of your regular mouth tall half pint jar and apply a thin bead of RTV silicone to the inside ring.

2) Add a small bead of silicone to the small suction hole that is built into the cup.
3) Let the silicone set for about 10 minutes. Then Press the lid against the silicone. After that use your finger to seal the silicone on the inside and outside of the lid. Use the tip of your pointing finger to steadily push the silicone into a flat position and work your way all around the lid. A smooth steady motion with the finger will make a cleaner seal than my poor workmanship shown in the image. But this still works perfectly. There are a number of videos on youtube on how to apply a good seal by finger for silicone.

4) The blue Injection port fits perfectly as a plug. Simply insert it and you are done. Let the silicone cure 24 hours prior to use. I also test the jar with liquid and see if it leaks prior to use. Sometimes you apply too much silicone and you can simply cut it down with a sharp knife or completely reapply if necessary. It may take a few trials to get your silicone application perfect. There is an art form to it. This is the pour lid complete.

Procedure for building the blade assembly
1) Take your regular mouth band and place a silicone bead around the edge of the inside of the band.

2) Wait 10 minutes and then press the metal blade assembly into the band.
3) Now place the grey gasket down over the silicone and press down to allow the gasket to set in. The gasket is necessary so that the jar seals properly when you are blending the jar. Otherwise it will explode or leak out of the jar during blending.

4) Use your finger to seal the silicone in place as mentioned above. Seal the inside and outside of the blender assembly.

5) Let the silicone cure 24 hours prior to use. I also test the blender assembly prior to use to make sure it fits the half pint mason jar and does not leak. I fill it with water and blend a few blends. If it leaks you may need to redo your silicone application or cut back some silicone if you applied too much.
Procedure for liquid inoculation
Recipe: One regular mouth 1/2 pint was filled half full with tap water (1/4 pint water to 1 Pasty plate).
1) Fill a half pint jar half full with tap water. The blade assembly is placed on top of the half pint jar and screwed on. I then place a piece of medium grade foil to cover the entire lid.

2) The pour lid is wrapped in foil. I use roughly the length of a medium grade foil roll by about 4 inches wide. I fold it down the middle. Take note, I place the open part of the lid down on the foil and wrap the foil over it.

I fold (3) 1/4” wide flaps on 3 sides and fold them over. This comes in handy when you unwrap the foil to place the lid on the mason jar for pouring. In addition, you can recycle the foil and use it anytime you cook the lid. I’ve used the same foil for about 3 plus years until it’s worn out and needs replacing.
 
This is how the lid is wrapped.

This is how the lid is unwrapped

The reason is you can unfold the sides without exposing the bottom portion of the pour lid before making your lid swap onto the blended mason jar for distribution. Both items were pressure cooked for 20 minutes at 15psi. I lump these pour lid and assembly items when I make pasty plates so you are not doing any extra pressure cooking. It can all be done at one time.
3)Once the pressure cooker is cool. Prepare your SAB for work. Place all utensils in your SAB. Foil covered pour lid, isopropyl soaked paper towel, colonized pasty plate, 1/2 pint mason jar with blade assembly topped with foil.
4) Plugin your Oster blender base near your work area. I use gloves and wipe down my hands up to my elbows with 70% iso before placing them in the SAB.
5) Now it is time to Tiger Drop your plate into the blender jar. Wipe the outside of your pasty plate and blender jar with the iso paper towel.
Take your finger and thumb and loosen the culture/agar from the bottom of the pasty plate. Remove the foil from the blender jar. Loosen the lids on both the plate and blender jar. I use my left hand to hold the pasty plate and my right to lift the lid of the blender jar.

I simultaneously open the blender jar and plate and drop the entire culture directly into the blender jar in one plop. The inside of blender blade lid is directly above the blender jar as you make the drop so when the blender jar lid is opened the lid acts as a barrier from things falling from above. This is one steady motion and the exposure of both the culture and the blender jar is milliseconds

Below is an illustration of how you would do this with a normal plate or holy grail plate. I simply flamed my exacto knife and cut around the culture. Then cut the culture into 4 equal pie pieces. I then drop the plate flipping it upside down into the blender assembly jar.

6) Screw the lid of the blender jar on tight. The jar is then taken out of SAB and placed on the oster blender base. The culture is blended on the oster blender unit for 3-5 seconds or until the entire plate and culture a puréed into a fine texture. Wipe down the outside of the blender jar with an iso paper towel. Place the blender jar back into the SAB.
The image below is an example of a 1/2 pint jar being blended on an oster blender. This is for reference to show you what it looks like.

This is what your liquid inoculate will look like after the blend. The mycelia pieces will settle to the bottom when you leave the jar alone.

7) Before you bring the jar back into the SAB make sure to wipe it down with an iso paper towel. Now it is time to swap your blender assembly for the pour lid. Loosen the lid of your blender jar so the swap is quick and easy. You loosen it enough to where you can lift the lid for the swap but leave the lid on top of the jar until the swap.


8) Below you can see how the foil is unfolded on two sides but left in place until the swap.

With my right hand, I lift the pour lid and with my left hand I lift the blender jar lid. I swap them at the exact same time so exposure is minimal. Now your jars are ready to inoculate. This is a quick time lapse of the lid swap.(im using my new stainless steel LI pour lids see above.)


9) I then load my grain jars into the SAB. I wipe each one down all over with an iso paper towel before it is placed into the SAB. I loosen each lid by hand. I let the air settle prior to doing any work.


12) Remove the plug before the pour. I Lightly swirl the liquid inoculate jar before each pour as the mycelia slurry settles. With my left hand I lift the grain jar lid, with my right hand I do a quick turn for a split second and then close the grain jar lid. While I do all this, the grain jar lid is held directly above the grain jar opening to act as a shield from anything falling from above. Grain exposure is minimal during the pour and basically the pour is done underneath the grain jar lid. With these new lids the spout has a small latch that closes between each pour for convenience. There is about 1cc volume distributed into each jar from the quick flick of the wrist.
Swirl technique and loosening the lid  Inoculation

13) As i am putting the jar down between each pour, im using my other hand to screw the lid of receiving jar back on. Tighten each lid. You can now remove the jars from the SAB to shake them.
14) Shake each jar thoroughly. The idea is that you shake and turn the jar as you are shaking. I almost do an upward motion while shaking so it folds the grain from bottom to top over the liquid inoculate. What this does is distribute all of the liquid inoculate evenly. The key to not having to shake later on is to spend the time shaking up front. I spend about one minute per jar. After that, no shaking necessary.
You can see a video below on pouring the liquid inoculant in a SAB by Carcase-x to illustrate the pour.
Below are some images of how it looks after inoculation. The jars were not shaken once the initial shake was complete.
Day 4 after inoculation

Day 7 after inoculation

The beauty of liquid inoculation is that if you shake properly right after inoculation you do not need to shake again. This saves you time and man power in the long run. Generally you will have colonized jars in 7-10 days and you are ready to spawn. I like the fact that all I have to do after inoculation is spawn. No shake necessary.

Here are a few sample grows using LI. Syzygy is shown below.
 
AA+

LGT

Tex

After your jars colonize it is time to prepare your bulk substrate.
EATS UNBUCK3T TEK
Edited by eatyualive (05/26/20 12:19 PM)
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fahtster
Now With 33%More Faht


Registered: 06/17/06
Posts: 9,269
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Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
#26356248 - 12/01/19 08:50 PM (4 years, 1 month ago) |
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This thread was moved from the user's journal.
Reason: Eats request
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: EAT LIQUID INOCULATES™ (moved) [Re: fahtster]
#26415747 - 01/04/20 09:35 AM (4 years, 25 days ago) |
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I’ve recently switched to the holy grail plates. I feel it’s a completely different technique as far as tiger drop. I did one batch yesterday and simply loosened the plate with a bernzomatic torch flame sterilized exacto knife and dropped it. While doing this I used the lid of the dish at a 45 degree angle to shield anything from above while I loosened the plate with the exacto.
I prefer the pasty plate because I can loosen it prior to opening and exposing the culture to the SAB. Then do a quick drop with minimal exposure during drop. However, what I may do is do my Initial transfers on the holy grail plates and do my 2Nd and 3rd transfer on pasty’s for ease of technique using a tiger drop.
I’ve also simply used a normal plate and cut 1/3 of the plate and dropped it in as well. It all works and you could use an entire plate but the pasty plate is well suited for the drop into the tall half pint due to size and technique.
It definitely was not as smooth as the pasty plate. But you give and take easy tiger drop for better visibility. The holy grail plates are also too hard to loosen the agar from the plate
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Astoriensis
Saprobe
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Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
#26415855 - 01/04/20 11:05 AM (4 years, 25 days ago) |
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Cool write up here 
I've been thinking of something similar to this but an all in one blender blade and injection port. Perhaps there is no room looking at your pix...
That way no messing with removing lids.
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FunnyFungiName
Ninja Space Pirate


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Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
#26415860 - 01/04/20 11:13 AM (4 years, 25 days ago) |
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Awesome write up! Have you tried larger jars like a pint or used 1 culture to 5 instead of 10?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Quote:
I've been thinking of something similar to this but an all in one blender blade and injection port. Perhaps there is no room looking at your pix...
We did this thing called a tribal container years ago. It had a blender and pour lid all in one. I was using it for a while but the bottom metal blade assembly are difficult to adhere to the plastic. The plastic and metal don’t like to stick together much so to say.
https://www.shroomery.org/forums/showflat.php/Number/21927954
Quote:
Have you tried larger jars like a pint or used 1 culture to 5 instead of 10?
Yes I’ve tried regular mouth pints, quarts ect. But they have to regular mouth. You can also buy the pour lid in wide mouth version but the blade assemblies then have to be attached to the lid and it is more difficult to do so.
My ratio is for 10 quarts. But I’ve done 20-30 quarts with one half pint. You could use a pint then you would have 1/2 pint water for inoculant. You can double up your quarts in that case. You don’t want to overpour though so the grain doesn’t get water logged with pooling water. After inoculation and shake you shouldn’t visibly see any pooling water. The inoculate water should be distributed evenly throughout the jar.


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TedsDead



Registered: 01/03/17
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Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
#26415947 - 01/04/20 12:13 PM (4 years, 25 days ago) |
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Quote:
eatyualive said:
We did this thing called a tribal container years ago. It had a blender and pour lid all in one. I was using it for a while but the bottom metal blade assembly are difficult to adhere to the plastic. The plastic and metal don’t like to stick together much so to say.
yeah, I still have a few of those. I really like the concept but the blades definitely dont like to stay attached I cant say ive been doing too much blending lately but theres a nice 1 piece pour lid with a gasket by "masontops" . works great for the pour without having to mod. only thing is although it claims PP5 they warp @ 20 psi so u gotta keep it at 15 if u use em . theyre like 5-8 bucks a piece so it hurts when they break
how snug does your rubber port fit into the hole in the lid eats? I have a couple that I may have sanded a bit too much. no good for the tribal container but may still work for this application
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: EAT LIQUID INOCULATES™ (moved) [Re: TedsDead]
#26416375 - 01/04/20 05:28 PM (4 years, 25 days ago) |
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Do you have a link to it? I’ll try it for sure! Rubber plug is very tight actually.
Look these are metal or stainless steel. No more building required. I’ll update the op when I test these. They look convenient between pours.
https://www.amazon.com/Infusions-Liquor-Closed-Dispenser-Regular/dp/B07LF5GYC5/ref=pd_aw_sim_79_8?_encoding=UTF8&pd_rd_i=B07LF5GYC5&pd_rd_r=793bf21d-c6c7-494b-8e9e-5cb8e9666e5d&pd_rd_w=gp4Wy&pd_rd_wg=IkweC&pf_rd_p=a966b482-aeee-4b16-9ffe-9a1cb857c30c&pf_rd_r=WZF9M3JM5KV1WM4TK5S0&psc=1&refRID=T33Y5ZE34D9FKPJH054G
I ordered 2. This is money!

Crap, look at the image of the bottom. That’s going to be an issue with clogging and stopping the blended up mycelial pieces. Fuck it I’ll still try.
Edited by eatyualive (01/04/20 06:15 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
#26416481 - 01/04/20 06:25 PM (4 years, 25 days ago) |
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YogiBear



Registered: 08/24/19
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26416506 - 01/04/20 06:42 PM (4 years, 25 days ago) |
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😎🍿
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: EAT LIQUID INOCULATES™ [Re: YogiBear]
#26416525 - 01/04/20 06:55 PM (4 years, 25 days ago) |
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So found a user image on the first one. It appears this may work. The smaller line appears to be an air intake. Which may or may not be an issue. I’d use the plastic lids with caps but I definitely don’t want to melt them in the cooker.
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Hillbillyjohn


Registered: 11/13/15
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26416531 - 01/04/20 07:02 PM (4 years, 25 days ago) |
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Damn I read the title and thought I was gonna get to see some fucktard chugging a jug of lc haha.
But actually I was just starting to read up on LC/LI so I'm glad I ran across it thanks for write up eat.
Edited by Hillbillyjohn (01/04/20 07:05 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
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Love that you’re still rocking the blender mods. I did have some success myself with them a few years back. Nice stuff man!
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: EAT LIQUID INOCULATES™ [Re: Pastywhyte]
#26416766 - 01/04/20 10:07 PM (4 years, 24 days ago) |
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Wish they had that lid 5 years ago
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26416827 - 01/04/20 10:57 PM (4 years, 24 days ago) |
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-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26416837 - 01/04/20 11:07 PM (4 years, 24 days ago) |
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I was looking at those initially. Very similar to the blender ball lids I’ve used. I’ve pressure cooker the blender balls with no issue. And I was actually thinking of trying those if the stainless steel don’t work. I definitely don’t want to melt them. I’m also concerned with the stainless steel assembly that the seal may melt. It doesn’t on the oster blender blade gasket but I guess we won’t know until it is tested. I’ll be the guinea pig.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26417174 - 01/05/20 08:47 AM (4 years, 24 days ago) |
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lol!
well, I only melted one once when I let my pc get over 20psi on the stove top. the metal ones look nice but the flow rate seems like it not be enough to pour quickly. if your attentive and watch your pc and dont get too stoned there shouldnt be any issues with melting. the one tht I "melted" . just warped a bit and looks ugly but is still fully functional. fack tho that website lists a higher retail than I paid at the store!?
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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StrohngBahd
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26417188 - 01/05/20 09:02 AM (4 years, 24 days ago) |
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So I just realized my magic bullet blender is pp5, I did a quick search and found some people who melted theirs in the PC but no mention if it was pp5 plastic. Anyone have recent experience using these?
-------------------- No Turn Un-Stoned
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TedsDead



Registered: 01/03/17
Posts: 4,998
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theyll melt. pretty sure theyre PET or PTF. says right on the magic bullet "not for hot liquids" so I def wouldnt fuck with it in a PC. you can tell at a glance that it doesnt even look like PP5 plastic
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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Bph
Stranger



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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26417251 - 01/05/20 09:54 AM (4 years, 24 days ago) |
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Anyone ever try it with a Ninja blender. I feel like its made of some bad ass plastic. I just use it to grind up weed and mush for chocolates.
Edited by Bph (01/05/20 09:56 AM)
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Bph
Stranger



Registered: 10/11/18
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Re: EAT LIQUID INOCULATES™ [Re: Bph]
#26417254 - 01/05/20 09:56 AM (4 years, 24 days ago) |
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Ment to post a picture
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: EAT LIQUID INOCULATES™ [Re: Bph]
#26417416 - 01/05/20 11:27 AM (4 years, 24 days ago) |
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Ok so at least the orange ones are a viable option if these metal pour spouts dont work.
Bph does it say pp5 somewhere on it?
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Bph
Stranger



Registered: 10/11/18
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26417440 - 01/05/20 11:46 AM (4 years, 24 days ago) |
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It's made if "tritan" by eastman plastics. Just says it's durable and won't warp in the dishwasher so I'm thinking its good... The wife will likely kill me if I melt it tho.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: EAT LIQUID INOCULATES™ [Re: Bph]
#26417455 - 01/05/20 12:01 PM (4 years, 24 days ago) |
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they don;t look like the'yll survive the pc. test: boil it in a pot on the stove and see how it holds up. if it comes out unscathed it may be up to a test in the pc but if it warps or cracks at all scratch it.
also with other brands of plastic lids that claimed PP5 I did find some colors to warp more/less than other colors. yellow and orange were the best and Ive reused them many times. purple and blue shrunk considerably. green and gray warped just enough that I didnt like how the threads sat after the pc cycle . (this is regular colored offbrand mason lids NOT the masontop brand pour lids) .
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
Edited by TedsDead (01/05/20 12:47 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26417497 - 01/05/20 12:35 PM (4 years, 24 days ago) |
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Thanks for the info!
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Bph
Stranger



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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26417562 - 01/05/20 01:29 PM (4 years, 24 days ago) |
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Found the Info. I forgot the word for it but it is rated to go up to 108c so not quite enough balls for the Pc cycle. May have to go to the Goodwill and snag a cheap blender. I've always liked the idea of getting a bunch of jars going with one clean plate.
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TedsDead



Registered: 01/03/17
Posts: 4,998
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Re: EAT LIQUID INOCULATES™ [Re: Bph]
#26417573 - 01/05/20 01:40 PM (4 years, 24 days ago) |
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you can still get a bunch of jars going with 1 clean plate. the colonization time will just take longer if your dropping wedges
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead] 1
#26417605 - 01/05/20 02:01 PM (4 years, 24 days ago) |
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Bph you want an oster brand blender. It comes with a blade attachment and they fit perfect on a regular mouth pint, half pint or quart. You also don’t want to use the black plastic base as it will melt in the pc.
Also I can easily knock up 30 jars with one plate and 1/4 pint water. You can use a regular mouth pint half full(1/2 pint of water) to one plate and inoculate close to 50 quarts with one plate. Granted you will have less inoculant but it will take longer to colonize. You could do a shake after a few days if you want. There is an art form to it. The more evenly you do your initial shake after inoculation the quicker your jars will grow. Spend a good minute shaking and turning each quart after inoculation. If time isn’t an issue simply inoculate more jars with less inoculant.
I don’t usually shake at all after the initial shake. But if I inoculate more jars with less inoculate, I may shake after 3 days to speed things up a little. My end goal is not to have to shake 50 quarts that’s a lot more work and adds up quick. If I can do anything to lessen shake time I’m down.
Edited by eatyualive (01/05/20 02:13 PM)
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Bph
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26417662 - 01/05/20 02:30 PM (4 years, 24 days ago) |
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All this blender talk put me in the mood for a margarita. And as for getting lots of jars from one plate using multiple small wedges. I find the more time a plate is open and the more times I'm in and out of it with my blade the more bacteria I get. I know that's my fault but I still am looking for ways to give myself an edge it would be cool get 30 clean shoe boxes off one plate.
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: Bph]
#26417693 - 01/05/20 02:52 PM (4 years, 24 days ago) |
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I’d smash myself in the face if I had to cut 20 wedges for a2g. Liquids I pour like a beer and it takes me about 10 minutes for 30 quarts.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26419056 - 01/06/20 10:28 AM (4 years, 23 days ago) |
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TedsDead



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thats what is being used on every build. its the pour lids that need to be true
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26419184 - 01/06/20 11:50 AM (4 years, 23 days ago) |
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Quote:
eatyualive said: I prefer the pasty plate because I can loosen it prior to opening and exposing the culture to the SAB. Then do a quick drop with minimal exposure during drop. However, what I may do is do my Initial transfers on the holy grail plates and do my 2Nd and 3rd transfer on pasty’s for ease of technique using a tiger drop.
That's an excellent idea, It would be awesome if the plastic of the HG plates were soft at the bottom!!! Damn, I was thinking updating the HG thread with that idea, just so the people know that they can do that as well!
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TedsDead



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Re: EAT LIQUID INOCULATES™ [Re: Mr. Alien]
#26419353 - 01/06/20 01:52 PM (4 years, 23 days ago) |
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also if you cut the wedges out first you can usually get them to pop out with a quick flick to the back of the dish( with HG plates) . not as easy as squeezing out the pasty plate but if one find themself in such predicament it may be a helpful technique
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26419793 - 01/06/20 06:27 PM (4 years, 23 days ago) |
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Yeah alien I wish I could pry the agar loose 8 tried already but couldn’t. Either way I love those holy grails. But I’ll keep pasty’s specifically for the drop.
Yeah funnyfungi im using that in the op. You can’t beat the price of Goodman’s and oster brand. So I’ve also gotten blender assemblies at Walmart. They hide them on the top shelf as a replacement blade that comes with a gasket. It’s 10$ though. I have 3-4 blades but really only use two back and forth.
I mean I think I can take my exacto blade, loosen the entire holy grail plate and drop it. But the exposure is a little more than it would be with a pasty plate. I can also use the bottom of the pasty plate as a shield as I lift the blade to drop the plate.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26419801 - 01/06/20 06:33 PM (4 years, 23 days ago) |
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Quote:
eatyualive said: Bph you want an oster brand blender. It comes with a blade attachment and they fit perfect on a regular mouth pint, half pint or quart. You also don’t want to use the black plastic base as it will melt in the pc.
Also I can easily knock up 30 jars with one plate and 1/4 pint water. You can use a regular mouth pint half full(1/2 pint of water) to one plate and inoculate close to 50 quarts with one plate. Granted you will have less inoculant but it will take longer to colonize. You could do a shake after a few days if you want. There is an art form to it. The more evenly you do your initial shake after inoculation the quicker your jars will grow. Spend a good minute shaking and turning each quart after inoculation. If time isn’t an issue simply inoculate more jars with less inoculant.
I don’t usually shake at all after the initial shake. But if I inoculate more jars with less inoculate, I may shake after 3 days to speed things up a little. My end goal is not to have to shake 50 quarts that’s a lot more work and adds up quick. If I can do anything to lessen shake time I’m down.
I just did this last week. Took a tiny cake and turned it into 3 gal of good spawn in 4 days. I shook the two on the left bc they had uncolonized grains and I wanted to spawn them over the weekend. Spawned 4 monos one week from using this tek. Never shook the jar in the right.
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eatyualive
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Ok here we go.
The lids are being wrapped in heavy duty aluminum foil. Make sure to be wary of the bottom air spout. This can poke a hole through the foil. I am pressure cooking the lid for 30 minutes at 15psi. And now it begins. Now it’s time for the elves.




We tested the pour lid two ways with 1/4 pint of water in a regular mouth half pint.
First one is the normal setting where the spout comes down about 1/4 inch below the bottom of the lid. This is how the lid is intended for use with oil. I’m assuming you want a delay with oil so a large amount doesnt come gushing all over the place. I also can’t get the remainder of the liquid out at the bottom of the batch and I feel it’s wasteful.

The 2nd way we put the spout flush with the bottom of the lid. This seems to pour a little better and more controlled. I’m not sure I like the cap it’s a little funky. But it does provide a nice cover between pours if you need to take a break and it’s automatic. You could use your gloved finger to push it closed as well. I want a quick motion where I flick once and it lets out as much inoculant as I need per jar. So what I’ve done in the image below is sit the spout flush with the bottom of the lid.
Edited by eatyualive (01/07/20 08:58 PM)
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NothingsChanged
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26424558 - 01/09/20 12:26 PM (4 years, 20 days ago) |
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--------------------

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eatyualive
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Ok test one with the bottom of the pour spout flush with lid is complete.
I do a slight swirl right before the first pour. If you keep the speed consistent after, the movement of the pour jar moves the mycelia slurry around for you. If you need to pause between jars for whatever reason. Simply do a light swirl again. I’ll work on getting a video of it. Things were foggy and hard to see with what I made.

So the pour was nice and controlled. It poured less water than I’m used to but I absolutely love the automatic closing lid between each pour. It’s also really convenient if you lift the lid of receiving jar at a 45degree angle turn the pour lid and pour. When you lift the pour jar up it automatically closes and as that happens you are closing the lid to receiving grain jar. I slightly tighten each lid after the pour with my left hand while I’m doing all pour work with my right.
What I didn’t like is that 90% of the jar pours but the last 10% won’t pour. Even if you flip the entire jar 180 to the surface of the work area. You would have to free pour and that is not the idea. I’ll likely just increase my volume of water 1/4 inch lore in the jar next time to account for the wasted liquid.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26438608 - 01/17/20 01:31 PM (4 years, 12 days ago) |
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Hi Eats,
I've been making LI in a similar way with golden teacher, noccing up gallon bags of wheat. But man, your leapoff is soooo much faster than mine. I see leapoff on day 2, but only barely.
I blend a 50cc worth of water and a full size agar plate, into a syringe and squirt that into two, 1 gallon bags of wheat. I blend a little less than you do I think. Some of the bigger pieces are the size of rice. I use a huge basting needle (maybe a 1/8 in diameter).
Any advice? Your jars just EXPLODE!!!
I only slosh the grains around for 20 seconds or so after nocc'ing, fearing damaging the mycelia even more by the mixing.... maybe thats my problem.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
Edited by tedoro (01/17/20 01:50 PM)
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SynKyd
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Liking this idea, just have to ask have you tried doing larger grain jars? Seems with this speed you could use the half gallon jars without stalling out.
Quote:
eatyualive said: I’d smash myself in the face if I had to cut 20 wedges for a2g. Liquids I pour like a beer and it takes me about 10 minutes for 30 quarts.
I pour beer in my mouth so.........
Quote:
Hillbillyjohn said: Damn I read the title and thought I was gonna get to see some fucktard chugging a jug of lc haha. .
Same, same!
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: SynKyd]
#26438667 - 01/17/20 02:10 PM (4 years, 12 days ago) |
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So here is my trial with the blender lid above. This is extremely low and slow leap off than I’m used to. Normally my jar would be 70-100% colonized by day 8.

What agar recipe are you using? I sincerely think leap off has more to do with texture and thickness of your inoculant than it does the actual amount of mycelia. For instance with pf jar slurries, the inoculant is so thick it literally sticks to every piece of grain after you shake it. So the jars colonize in 3-4 days to 100% with less than 1 cc of inoculant. Whereas you see in my example above I used a standard 1/3 of a regular petri plate and the leap off was slower. Granted I did use less inoculant due to this lid having leftovers and not allowing me to use 10% volume of the inoculant.
In addition, cornmeal plates are 2nd fastest to brf slurries. They are very thick and muddy when poured and it sticks to the grain better. You have Lcs which may slip past the grain and not catch as well.
I used pasty plates in the op. Even with the almost 90% colonized plate, it is still about the same size of mycelia I’m using from 1/3 of a Normal petri plate since they are much larger. The pasty recipe is potato flakes, karo syrup and agar. Sugar definitely helps in leap off. And I have to say, I used to use popcorn grain and leap off was tremendous. I highly believe the difference you are seeing may have something to do with starches and sugars. But let me know what your using?
Another big tip is shaking the jars for at least one minute minimum to distribute the inoculant even. I have jars that take much longer in my batches when I don’t shake them initially as even. Technique is key.
I’m working on some videos or gifs of the shake and pour so you can clarify what I am doing.
Edited by eatyualive (01/17/20 02:27 PM)
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26438732 - 01/17/20 03:05 PM (4 years, 12 days ago) |
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I use 11g to 400 ml water of this malt extract agar. Its what I came up with for just soft enough for LI and some more aggressive growth.
I rather like standard plates, and would like to continue using them if possible.... can I learn to use cornmeal plates with standard plates? Would that be a good move?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26438740 - 01/17/20 03:11 PM (4 years, 12 days ago) |
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Nah just use that. Add a little karo syrup to your mix specifically for plates you are using for LI and see if that helps.
Also I used to use popcorn grain as spawn. It has a lot of extra starches on the grain compared to most. But compared to wbs and oats it has much faster leap off from standard g2g.
Are you free pouring?
Also another thing, in jars I feel it’s easier to shake and get even distribution of your inoculant after a shake compared to a bag. If that makes any sense.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26438744 - 01/17/20 03:14 PM (4 years, 12 days ago) |
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If I make 400ml batches, where would you start at with the amount of Karo? and what would be things to look out for? Contams if too much? No improvement if too little?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26438752 - 01/17/20 03:17 PM (4 years, 12 days ago) |
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I'm also wondering if during my syringe noc'ing, if I couldn't squirt it around more evenly, assisting in the mixup.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26438755 - 01/17/20 03:18 PM (4 years, 12 days ago) |
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So I generally use the standard pasty recipe of 1 cup water to 2 tablespoons agar and 2 teaspoons of potato flakes. I just eye one big glob of karo syrup in that mix. This makes 8 plates.
Maybe change up your recipe only for what you are liquid inoculating to see if there is a noticeable difference. This seems to work really well for me. You could probably add a big glob squeeze out of the bottle to that volume.
Are you using the syringe to inject into bags? I think it may also be that you are using bags and I’m using jars. So when I shake really well, my Inoculant is touching every piece of grain with a good shake.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26438763 - 01/17/20 03:22 PM (4 years, 12 days ago) |
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Will do. I'll report back. This is exciting to think I could get my leapoff to look half as good as yours. Doing a ton of agar work on Sunday.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26438771 - 01/17/20 03:28 PM (4 years, 12 days ago) |
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Eats,
I just did some rough measurements and it looks like a pastyplate has over an ounce of agar where my plates I pour as thin as possible... maybe a 1/3 of an ounce. Maybe I'd be better served pouring them thicker so my LI is thicker. I make 20 plates with that 400ml.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
Edited by tedoro (01/17/20 03:30 PM)
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26438809 - 01/17/20 03:50 PM (4 years, 12 days ago) |
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Yes I would agree to try that as well. The agar is sticky and helps the mycelia kinda catch on to the grain. My LI looks like chunky soup when I pour it compared to a plate I used with a standard agar plate. The thicker the better. Take a look.
This is a pasty plate blended to LI.

This is a cornmeal plate LI

This is a pf jar slurry. As you can see it’s solid like mud and coats all the grain upon inoculation. This means it has far more inoculation points thus colonizes twice as fast.
Edited by eatyualive (01/17/20 03:58 PM)
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26438815 - 01/17/20 03:53 PM (4 years, 12 days ago) |
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This is great. I have something to aim for. Many thanks for the time.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26438908 - 01/17/20 05:00 PM (4 years, 12 days ago) |
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Will any oster base work or is it a certain model?
Cutting colonization time by like 2/3s with this rig might be the greatest cult tool ever.
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26438918 - 01/17/20 05:05 PM (4 years, 12 days ago) |
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Yes any stainless steel oster blender base should work unless its some fancy smoothie kinda thing. Dont pc the black plastic bottom piece because it will melt. I used to simply wipe it with iso and use the regular lid and free pour. Then when i was on this forum, i was told it was bad technique. Seemed to work for me for years. But the gasket can get tangled up after a blend so the pour lid is a cleaner and an easier way of pouring and evenly distributing your LI to jars or bags.
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26440401 - 01/18/20 03:05 PM (4 years, 11 days ago) |
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For reference. This is day 9 on both sets of jars side by side.
Stropharia: normal plate blended LI. I dont know the agar mix but it isnt the same as the mix below. Im also trying this new lid that doesnt dispense as much liquid when pouring. I had to shake these jars to speed them up.

Stropharia: pasty plate blended LI. Normal pouring lid in op. You can see the jars above that were shaken in this pic on the left.
Edited by eatyualive (01/18/20 03:36 PM)
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26440494 - 01/18/20 04:26 PM (4 years, 11 days ago) |
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Excited to make a bit sweeter of a leapoff LI stew. Gunna mark the sweetened malt agar with a color.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26445196 - 01/21/20 08:32 PM (4 years, 8 days ago) |
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Im updating the op with some pour test gifs. This is the pour test with the spout flush with the bottom of the pour lid.

Also updated this with some gifs of the inoculation, lid swap, and swirl technique.
8) With my right hand, I lift the pour lid and with my left hand I lift the blender jar lid. I swap them at the exact same time so exposure is minimal. Now your jars are ready to inoculate. This is a quick time lapse of the lid swap.(im using my new LI pour lids see below.)

9) I then load my grain jars into the SAB. I wipe each one down all over with an iso paper towel before it is placed into the SAB. I loosen each lid by hand. I let the air settle prior to doing any work.

12) Remove the plug before the pour. I Lightly swirl the liquid inoculate jar before each pour as the mycelia slurry settles. With my left hand I lift the grain jar lid, with my right hand I do a quick turn for a split second and then close the grain jar lid. While I do all this, the grain jar lid is held directly above the grain jar opening to act as a shield from anything falling from above. Grain exposure is minimal during the pour and basically the pour is done underneath the grain jar lid. With these new lids sheon it has a small latch that closes between each pour for convenience. There is about 1cc-3cc volume distributed into each jar from the quick flick of the wrist. Put the lid back on loosely. We will tighten all the lids at the end.
Swirl technique
 Inoculation
Edited by eatyualive (01/22/20 05:55 AM)
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fahtster
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26445444 - 01/22/20 12:00 AM (4 years, 7 days ago) |
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Loving the gifs!
Faht
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Re: EAT LIQUID INOCULATES™ [Re: fahtster]
#26445454 - 01/22/20 12:14 AM (4 years, 7 days ago) |
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-------------------- Tek's I use LAGM2020
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eatyualive
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Yeah i have an entire video of the cut, drop and inoculation but i dont wanna deal with youtbe ect. They are far too long to make 100 gifs lol
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26455719 - 01/27/20 06:17 PM (4 years, 2 days ago) |
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Noc'd up eight wheat bags today with thickly poured, karo sweetened soft agar I blended and sucked up in a syringe. This is exciting. 45cc in each. some with 1/2 plate, some with a whole plate. My last bags with thin, no karo plates colonized in 8 days.
You can see the chunks of agar on the berries. I was able to suck up almost all of the broth.
I mixed the bags for 2 whole minutes each. taped the heat sealed corners to avoid a tear.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
Edited by tedoro (01/27/20 06:23 PM)
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26455855 - 01/27/20 07:31 PM (4 years, 2 days ago) |
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Yeah that looks thicker. I like those quarter pint jars you are using. I do prefer the larger though. Also dont be afraid to let that plate grow 80-90% before you LI it.
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26455887 - 01/27/20 07:47 PM (4 years, 2 days ago) |
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Roger all that. I scheduled it a few days ago, but my MS from spores (4th transfer) is slow growing. Maybe thats just how it is, or maybe my soft. sweet agar does't give it much reason to hurry and colonize the plate.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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murderlabz
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26456005 - 01/27/20 08:43 PM (4 years, 2 days ago) |
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: murderlabz]
#26462064 - 01/31/20 12:28 PM (3 years, 11 months ago) |
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More of my plates after a few more days.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
Edited by tedoro (02/01/20 11:18 AM)
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TedsDead



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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26462789 - 01/31/20 08:20 PM (3 years, 11 months ago) |
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any of you guys know what those weird blank "sectors" are? Im thinking its a contam but not sure what. it seems tricky to transfer away from sometimes. always seems to pop up on ms from time to time
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26462794 - 01/31/20 08:27 PM (3 years, 11 months ago) |
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I’m wondering the same thing. I’ve only seen it recently when I started using spores.
This is a sagari plate I made, myc is running the whole thing down to the bottom. Should be perfect but I’m surprised it wasn’t mentioned at all in the sagari/li threads.
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26462796 - 01/31/20 08:30 PM (3 years, 11 months ago) |
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yeah, that what im saying I always see it when starting new spores. you can get away from it easily enough but why wont the myc touch those spots?
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26463205 - 02/01/20 08:14 AM (3 years, 11 months ago) |
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I actually thought tedoro transferred slices out in those areas.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26463279 - 02/01/20 09:18 AM (3 years, 11 months ago) |
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Eat you never seen that before??
It’s for sure something with the spores I did a bunch of clones prior to LAGM and never encountered that.
Then my germ and T1s for pesa and aa both look like that.
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26463309 - 02/01/20 09:39 AM (3 years, 11 months ago) |
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Ive seen it maybe two times randomly. Nothing consistent like that.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26463337 - 02/01/20 09:57 AM (3 years, 11 months ago) |
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I see it quite often on ms plates. something from unclean prints I imagine but I really dont know. I thought it was just charactreristic of a certain variety for a while but once I started seeing it on other ms plates it occurred to me that all of the plates of said variety had also been ms. I mustve started like 15 different varieties this month and saw it on alot of the plates across different varieties
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26463467 - 02/01/20 11:17 AM (3 years, 11 months ago) |
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Well I'm not going to use them (I used their brothers on a few bags, thats risky enough) I made transfers from them today.
Those bags from said funny looking red plates..
Day 5

I could barely see any leap-off at day 3... but then it explodes.
Left bag is at 47% percent water content. Right one is at 49% and was boiled just to the point the wheat berries were bursting and when I drained them, it was a little starchy and continued to dribble for a long time. Seems like a little extra water and a little starch on the grains speeds things. But I don't think the Karo I added sped leap-off. I'm going to stick with MEA for now.
I'm really wondering if letting the syringes sit at room temp for three days to recover might speed the recovery. That blender is brutal. (but so is shaking the bags for 2 whole minutes)
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26463524 - 02/01/20 11:53 AM (3 years, 11 months ago) |
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i mean, you might as well consider those 3 days part of your recovery time as well... right?
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26463603 - 02/01/20 12:33 PM (3 years, 11 months ago) |
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Tedoro do you think the thicker LI solution helped?
Did you pour more LI into the one on the right side?
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26463734 - 02/01/20 01:50 PM (3 years, 11 months ago) |
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I'm trying to have a workflow that is fast. I don't mind doing the syringes 3 days earlier.... also, I want grains to be uncolonized for as little time after the PC'ing as possible.
I squirted the same amount in both bags. (1/2 agar plate in each)(I did eight bags total, between 47%-49%) and its the longer cooking, more water that sped the growth...... but Stamets recommends 47% for rye with my size bags.... so I kinda feel like at some point I'll be getting too wet for a large bag. My gut thought is wetter goes faster at a cost of being more bacteria prone. To a point, then too wet slows it all down.
Definitely the thicker poured plates helped. I didn't even let the plate grow out much, and every single little piece of agar grew mycelia. I don't think the Karo did much. 1 part agar 1 part malt 2 parts Karo.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
Edited by tedoro (02/01/20 03:02 PM)
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26463745 - 02/01/20 01:58 PM (3 years, 11 months ago) |
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Hey eats. Thanks so much for this thread. I'm really excited that my silly refusal to get a flowhood has a working solution. I'm growing out really great bags and I just realized that now that I have pre-sealed PC'ing down.... I can do 8 smaller, 2 quart bags in my presto... to grow out shoeboxes that are knocked from plates using LI, to test out strains. Its pretty sweet. I'm even soon going to start reusing bags.... considering I used to PC for 4.5 hours, I figure two, 2.5 hour runs ought to be okay.
Anyway, I'm rambling... thanks for this and all the help.
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26463922 - 02/01/20 03:55 PM (3 years, 11 months ago) |
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I actually used to reuse bags ehen i used to do them. Just reseal with the sealer. Good luck. Its all snout finding that perfect recipe for the variables you are using. I also think jars colonize a little faster because when you shake them, you almost hit all sides of the grain with the LI. I always find the thicker solutions do the best. I just use karo in my pasty plates and noticed they leap off much faster than other normal agar plates ive used.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26463971 - 02/01/20 04:21 PM (3 years, 11 months ago) |
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Quote:
eatyualive said: I actually used to reuse bags ehen i used to do them. Just reseal with the sealer. Good luck. Its all snout finding that perfect recipe for the variables you are using. I also think jars colonize a little faster because when you shake them, you almost hit all sides of the grain with the LI. I always find the thicker solutions do the best. I just use karo in my pasty plates and noticed they leap off much faster than other normal agar plates ive used.
Maybe I'll try a bit more karo. Its still just nothing for the first 3 full days. In the past I've read people barely blending and having faster leapoff, but man, having all of that agar blended to make a thick broth makes it really really good on day 5.
I mean I'm happy with these 7-8 day colonization of a huge grain bag.... just wanna make sure I can't improve it even more.
When I get a chance I'll make a thick LC and compare. I'm just so lovin these LI for all the LI reasons.
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26464018 - 02/01/20 04:53 PM (3 years, 11 months ago) |
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LI 4 LIFE! 
I do love slurries but i dont need 200 quarts in 4 days. LI can be tailor made for any size and volume. Tis why i like it so much. I used to simply blend up and suck into syringes. Then inject one syringe per 2-4 pf jars. A few days later slurry those into quarts. But i seemed to always have the same problem. Too much spawn too fast. Its a good problem to have.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26464307 - 02/01/20 08:16 PM (3 years, 11 months ago) |
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Okay, I don't know what I'm doing... but I made two 100cc LI syringes and I'm going to see what happens if I let them rest for a few days. Maybe the agar will jellify... maybe the broth will grow mycelia LC styles. Just an experiment.
Pasty made a ezLC tek similar... he says 7-10 days.
"You will soon notice that the nutes from the agar puck leach into the water (as will the food coloring if you use it) but any sediment or solid matter remain trapped in the agar puck. Give it some time to colonize, usually will take a week to 10 days. Swirling it aggressively will help expand the colony faster. You might dislodge the agar but that is not an issue."
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26464322 - 02/01/20 08:34 PM (3 years, 11 months ago) |
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You can save them for years and it wont make much difference. Ive stored LI syringes for 7 years and had success growing them out. I dont see any difference in speed. But i also keep them in the fridge.
7 year old LI
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26464328 - 02/01/20 08:42 PM (3 years, 11 months ago) |
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wow. 7 effin years.
Cool.... I'll do room temp for a few days, see what happens.
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26464366 - 02/01/20 09:29 PM (3 years, 11 months ago) |
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Quote:
tedoro said:

More of my plates after a few more days.
Burma?
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Re: EAT LIQUID INOCULATES™ [Re: Nichrome]
#26464413 - 02/01/20 10:24 PM (3 years, 11 months ago) |
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Quote:
Nichrome said:
Quote:
tedoro said:

More of my plates after a few more days.
Burma?
3rd transfer of MS golden teacher on very soft MEA plates that I spiked with Karo.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26464438 - 02/01/20 11:01 PM (3 years, 11 months ago) |
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Nice! I've got a burma that gets all pizza pie looking like those. None of my GT have acted like that but GT has been around forever so I'm sure there are various sets of lineage at this point. Looks beautiful!
Does your GT grow very many pebbles or blobs when it pins?
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Re: EAT LIQUID INOCULATES™ [Re: Nichrome]
#26464463 - 02/01/20 11:26 PM (3 years, 11 months ago) |
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Quote:
Nichrome said: Nice! I've got a burma that gets all pizza pie looking like those. None of my GT have acted like that but GT has been around forever so I'm sure there are various sets of lineage at this point. Looks beautiful!
Does your GT grow very many pebbles or blobs when it pins?
not sure what a pebble is, but I can imagine. nope... no blobs or none traditional mushrooms.
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26464483 - 02/01/20 11:49 PM (3 years, 11 months ago) |
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Here are some GT pinsets with the "pebbles" I referred. The ones that do this always kick ass.

This was LC to grain to bulk.
-------------------- “Better to be deprived of food for three days, than tea for one.”
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Re: EAT LIQUID INOCULATES™ [Re: Nichrome]
#26464647 - 02/02/20 04:53 AM (3 years, 11 months ago) |
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Like white blobs on your plates? Do they ever mature into pins if you let them go?
Edited by eatyualive (02/02/20 05:33 AM)
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26466701 - 02/03/20 11:49 AM (3 years, 11 months ago) |
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Day seven. How does this look? These .5 bags dry out the top surface but they fully colonize, just takes an extra day. I could move to .2 bags, I think I can easily pre-seal them too, now that I get pre-sealing.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
Edited by tedoro (02/03/20 12:05 PM)
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26466826 - 02/03/20 01:33 PM (3 years, 11 months ago) |
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It’s the filter. Ive heard of several people using the .2 just for that same reason. Looks good almost there. Fairly close to 7-8 days. I’d say you found the right volume. Stick with that formula you just used unless you are searching for a little bit faster speed. What was your normal time for colonization in those bags?
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26466842 - 02/03/20 01:46 PM (3 years, 11 months ago) |
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Crazy... something makes the red go away... maybe the mycelia is eating it? The plate is evenly thick and was all red. No uneven lighting to fade that side.
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26466846 - 02/03/20 01:49 PM (3 years, 11 months ago) |
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Yes mycelia will colonize the agar all the way through and it will lose its color over time.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26466865 - 02/03/20 01:58 PM (3 years, 11 months ago) |
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Quote:
eatyualive said: Yes mycelia will colonize the agar all the way through and it will lose its color over time.
Thats good news for people making LI. My transfers from these are looking better.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26467597 - 02/03/20 09:29 PM (3 years, 11 months ago) |
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Quote:
eatyualive said: It’s the filter. Ive heard of several people using the .2 just for that same reason. Looks good almost there. Fairly close to 7-8 days. I’d say you found the right volume. Stick with that formula you just used unless you are searching for a little bit faster speed. What was your normal time for colonization in those bags?
I was happy with two weeks before, My plates were poured very thin.... and I didn't boil the shit out of the wheat berries, which seems to help.
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26467642 - 02/03/20 09:54 PM (3 years, 11 months ago) |
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I keep all my grain on the dry side. Specifically for LI. Ive been working on a ton of gif videos of each step. I have them but I’m having issues uploading. I’ll update as soon as I can get it working. I got a video from above of the lid swap, unscrewing receiving jar lid, inoculation, unwrapping the foil lid.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26468079 - 02/04/20 08:20 AM (3 years, 11 months ago) |
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just dont make it so dry that the inside of the grain doesnt fully hydrate and contams your LI when you blend it up and expose the starchy insides did that a bunch when I was first prepping milo. didnt know I was ever underhydrating the grain until I tried blending some for LI.
0.2 pre-seal just fine. I like the .5's cause u can smell clean or dirty spawn theough them a bit better. 0.5's dry out a bit more in the winter months tho
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#26470829 - 02/05/20 06:04 PM (3 years, 11 months ago) |
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All I do is let the grain strain until it’s dry with no moisture on the outside. Then pc an 1 hour 15 mins. I could get away with 1 hour and I did for years and years. Recently I started cooking for 15-30 extra but I see no difference in contam rate or anything.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26470860 - 02/05/20 06:25 PM (3 years, 11 months ago) |
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alright, Im growing out a couple plates for an LI again. you guys reelled me in.
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead] 1
#26477366 - 02/09/20 06:08 PM (3 years, 11 months ago) |
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I got some top view gifs of a few of the techniques described in the op. I’m currently updating step by step with gifs.
 
Edited by eatyualive (02/09/20 07:08 PM)
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26478294 - 02/10/20 10:15 AM (3 years, 11 months ago) |
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Cool gifs!! Thanks for doing that!

I let my LI sit out at room temp for 8 days. Didn't see a great deal of growth, maybe some? Hard to tell when it was mostly stuffed with LI already.
Leapoff clearly seen the next morning (20hrs) when photo was taken. My grains usually take 4 days to look like this. Pretty cool. A little odd that some of the agar pieces leapt, and others didn't. Maybe the agar that was colonized thoroughly were the ones to leap. This was an old control bag from months ago that smelled good, but was a little dry. So I don't think it will mind 90cc's of LI. I'll keep y'all posted.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
Edited by tedoro (02/10/20 12:13 PM)
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26478310 - 02/10/20 10:29 AM (3 years, 11 months ago) |
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Nice!
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26492858 - 02/19/20 05:50 AM (3 years, 11 months ago) |
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Two questions:
1) If no pours are thicker, and thicker plates promote slower, less ropey growth, should one mix up less nute agar to keep ropey growth? (in my case thick poured petris)
2) When blending LI... is fluffy or ropey more frail to the blender blades?
(my apologies, always forget how to spell technical terms for ropey and fluffy)
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26493018 - 02/19/20 08:28 AM (3 years, 11 months ago) |
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It’s the same for me whether mycelia is tomentose or rhizo. I don’t give that much thought just blend and pour.
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26493094 - 02/19/20 09:23 AM (3 years, 11 months ago) |
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No pours are however thick you make them right
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26493136 - 02/19/20 10:00 AM (3 years, 11 months ago) |
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Quote:
A.k.a said: No pours are however thick you make them right
I don't make them... (I pour thick plates for LI) But I was reading that thinner plates can help push mycelia into rhizo growth...
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26493643 - 02/19/20 03:56 PM (3 years, 11 months ago) |
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So by no pour do you mean inject with a syringe?
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26493768 - 02/19/20 05:27 PM (3 years, 11 months ago) |
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I'm trying to get to the best method of making thick petri dishes for Blending to LI. The last dishes I made were thick and soft and tomentose... and I was wondering if I grow some cubes out on thin hard dishes, get them ropey.... how I might keep them ropey on my thick plates. (I like them thick so there is a ton of agar in my syringe)
I like them thick.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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fahtster
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26493930 - 02/19/20 06:55 PM (3 years, 11 months ago) |
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That’s what she said
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: fahtster] 1
#26493970 - 02/19/20 07:23 PM (3 years, 11 months ago) |
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Aa+, rw, wg are all tomentose. Ive never seen a rhizo with any of these and they perform well. 6-7z dry per flush average.
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26493988 - 02/19/20 07:40 PM (3 years, 11 months ago) |
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Thats cool to know. I've have speedier grows when my GT are ropey.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26494926 - 02/20/20 10:57 AM (3 years, 11 months ago) |
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Myc is myc right it’s all getting busted up in the end anyhow.
Dude no joke about aa+ being tomentose, it took three transfers off the germ plate until it wasn’t just a big cotton ball looking mess. I do finally have a nice rhizo plate of it going now though.
I was really impressed with how effective li is, I just hit up my first jar with it last week and it took a while to recover but when it did the whole jar blew up.
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LAGM2020     
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spiritlands



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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26497974 - 02/22/20 05:06 AM (3 years, 11 months ago) |
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AA+ slurryinahurry tek. First time ever fruiting cubes in a mono.

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eatyualive
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Nice job! You got a top layer on those? They look a little dry.
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MycQueen
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26515279 - 03/03/20 07:09 PM (3 years, 10 months ago) |
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I have several good clean varieties on agar that I want to blend & inoculate into WBS grain jars in one mega session, but I only have one blender blade assembly . Which of the below is best practice or acceptable (if none of the below or you have a better idea, please share); A] Use several jars of sterile water with bleach or IP alcohol added after PCing, as a sanitizing blade rinse between each variety? (what % to use?) B] Re wrap in foil & PC blade assembly between each variety? (how long?) C] Buy one blade for each variety? (know where I can get a deal on a dozen oster blades/seals ASAP?) Thanks
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: MycQueen] 1
#26515323 - 03/03/20 07:29 PM (3 years, 10 months ago) |
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MycQueen
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26515385 - 03/03/20 08:01 PM (3 years, 10 months ago) |
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Thanks Eat, you're the best!
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Sockadin



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Re: EAT LIQUID INOCULATES™ [Re: MycQueen]
#26515425 - 03/03/20 08:21 PM (3 years, 10 months ago) |
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OMG how far this idea has come. This is a beautiful write up. Can't believe it has taken me this long to read it... I remember back in the day when we chatted about the idea and I tried to do one but had to abandon the concept because I moved and didn't have time to continue cultivating.
Nicely done!
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MycQueen
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26523535 - 03/08/20 08:26 AM (3 years, 10 months ago) |
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Quote:
3) Now place the grey gasket down over the silicone and press down to allow the gasket to set in. The gasket is necessary so that the jar seals properly when you are blending the jar. Otherwise it will explode or leak out of the jar during blending. https://www.shroomery.org/forums/thumbs/15-002/095426404-thumb_SLURRYLID1.jpg
That looks like a wide mouth blender lid set up on the right (I love wide mouth jars). I know a regular mouth jar fits an Oster, but which blender fits a wide mouth jar?
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Sockadin



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Re: EAT LIQUID INOCULATES™ [Re: MycQueen]
#26523570 - 03/08/20 08:45 AM (3 years, 10 months ago) |
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You can also get regular mouth jelly jars. They work well with the oyster
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26606497 - 04/16/20 07:12 PM (3 years, 9 months ago) |
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Eats~
I've been having variable leap off with my LI, then I had an amazing batch that colonized an entire 4 quart bag of wheat in 5 days. But I seem to have slower colonization with dishes that race across the agar. I'm thinking that its very helpful if the agar gets colonized below the surface. I'm at 2 parts LME to 3 parts agar, made a bit soft, this makes it race and the plates look pretty, but maybe I should up the sugar to slow the growth and maybe get it deeper in the agar?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26606513 - 04/16/20 07:21 PM (3 years, 9 months ago) |
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I use pasty plate recipe. I see a consistent growth timeframe of 7-10 days max. My plates are thin and I don’t even let the plate colonize the surface completely. It usually doesn’t start colonizing under the agar until it runs the plate. I’m only leaving a 1/4 inch around w culture from the edge of plate. I don’t know if this helps, I use a big glob of karo syrup by eye. One big glob per 8-10 plates. Now I’m using 1 gram agar powder to this recipe because 2 grams is so solid I can barely cut it with the scalpel.
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FooMan



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Re: EAT LIQUID INOCULATES™ [Re: Bph] 1
#26609793 - 04/18/20 07:50 AM (3 years, 9 months ago) |
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Quote:
Bph said: It's made if "tritan" by eastman plastics. Just says it's durable and won't warp in the dishwasher so I'm thinking its good... The wife will likely kill me if I melt it tho.
The plastic should say dishwasher AND microwave safe. Dishwasher safe does not equal PC safe. That thing will melt in your PC guaranteed. Trying to save a marriage here
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Quick WBS Prep
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: FooMan]
#26633266 - 04/27/20 11:37 PM (3 years, 8 months ago) |
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You know when you have an idea and you think its smart, but then you realize others have definitely tried it before you....
Has anyone made LI with some water that is slightly sweetened, say with LME? making a poke inoculation super explosive?
Save me some time here... shoot me down.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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poisoned
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633678 - 04/28/20 06:39 AM (3 years, 8 months ago) |
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That's basically a LC then
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: poisoned]
#26633725 - 04/28/20 07:27 AM (3 years, 8 months ago) |
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Quote:
poisoned said: That's basically a LC then
yeah, but is it? With LC, you have all of this uncolonized nutrient, waiting for days to get colonized with a small piece of inoculate.
With this... its a whole colonized plate of mycelia, blended and used immediately.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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A.k.a
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633729 - 04/28/20 07:31 AM (3 years, 8 months ago) |
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I’m not getting it.
A whole plate of blended myc is just the blender li tek right?
Or like the super soft plates that dissolve during shaking.
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LAGM2020     
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poisoned
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26633746 - 04/28/20 07:45 AM (3 years, 8 months ago) |
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But why sweeten then?
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: poisoned] 1
#26633781 - 04/28/20 08:12 AM (3 years, 8 months ago) |
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Quote:
poisoned said: But why sweeten then?
I am trying to chase down why I am having varying leapoff from my LI. And one hypothesis is that the wheat berry bags that had more exploded kernels spread a bit of sugar on the external hulls of the berries...
... so I got to thinking that maybe if I added a bit of sweetening to my LI, I could reproduce the effect without intentionally bursting kernels.
* its not just the blender tek... I'm proposing adding nutes to the blender water
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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A.k.a
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Re: EAT LIQUID INOCULATES™ [Re: tedoro] 1
#26633782 - 04/28/20 08:13 AM (3 years, 8 months ago) |
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Give it a shot man it might speed things up.
One way to find out.
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LAGM2020     
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poisoned
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26633797 - 04/28/20 08:21 AM (3 years, 8 months ago) |
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Make sure to post the results. I'm still figuring out how low should I go with agar. Made it at 0.7% last time, waiting for plates to colonize. It didn't really liquefy at 1% and I had glass shards in that jar. I guess I have some super strong agar here.
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A.k.a
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Re: EAT LIQUID INOCULATES™ [Re: poisoned]
#26633804 - 04/28/20 08:24 AM (3 years, 8 months ago) |
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For li plates I used 3-4g agar to 450ml.
I’d make them slightly on the thinner side cuz when you pick a piece up it’ll split in half from its own weight if the agars thicker.
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LAGM2020     
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26633813 - 04/28/20 08:26 AM (3 years, 8 months ago) |
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Quote:
A.k.a said: For li plates I used 3-4g agar to 450ml.
I’d make them slightly on the thinner side cuz when you pick a piece up it’ll split in half from its own weight if the agars thicker.
cool. and 1% nutes?
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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A.k.a
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633826 - 04/28/20 08:33 AM (3 years, 8 months ago) |
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Idk usually like 2-4g lme also.
It’s been a minute since I did them but I remember making some with so little agar that instead of setting it made like a syrup.
I keep meaning to go back and make it again cuz it would probably work really well if you dropped a wedge in it and let it colonize.
Even with the soft plates I got a lot of myc growing down into the agar. It looks weird but packs way more myc per same amount of agar than a normal plate.
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LAGM2020     
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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: A.k.a]
#26633878 - 04/28/20 09:01 AM (3 years, 8 months ago) |
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I've been doing some sampling of fully colonized 2% agar plates... and I'm starting to think subsurface mycelia colonization is common with most agar recipes.

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tedoro
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633880 - 04/28/20 09:01 AM (3 years, 8 months ago) |
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what I'm not sure of is the density of that colonization.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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poisoned
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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26633934 - 04/28/20 09:29 AM (3 years, 8 months ago) |
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Yeah, I always get fluffy growth on the other side of agar puck next day when doing A2G.
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eatyualive
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Re: EAT LIQUID INOCULATES™ [Re: poisoned]
#26667179 - 05/12/20 06:15 PM (3 years, 8 months ago) |
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Ted check it out. Tested out a new toy today. It didn’t melt at 15 psi for 30 mins, but it wasn’t closed when I opened the foil. I was thinking it might have warped a little but when I pressed in the cap it was fine. I like the extra protection after each pour. These are nice.

Also a pointer for anyone having issues blending plates. I usually put mine on the liquify setting as it’s the strongest setting on my oster. But I had a fairly thick colonized plate today that wouldn’t break up. So I changed speed to the normal blend setting and it did the trick. So mixing up speed settings on the blender can pulverize the plate of you are having difficulty.
Edited by eatyualive (05/12/20 06:23 PM)
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eatyualive
Eat's You Alive :)



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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26683352 - 05/20/20 01:18 PM (3 years, 8 months ago) |
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So I had a new record for LI this week. I actually aplplied less inoculant per plate culture. I did roughly 15 quarts to one holy grail plate and the jars colonized in 4 days! I usually do 10. Might be these new pour lids getting a nice even pour.
This was APEU that doesn’t normally colonize this fast but it did. One thing is that a good shake makes the difference. I think I shook nicely and got the speed I always wanted with less inoculant using LI. Generally I’ve only had this speed with cornmeal agar plates. 
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ouuwee
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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26683466 - 05/20/20 02:19 PM (3 years, 8 months ago) |
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Can you PC those plastic lids? I'm looking to replace the original lids to plastic but the ball brand plastic lids i've found on amazon states their not used for canning
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eatyualive
Eat's You Alive :)



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Re: EAT LIQUID INOCULATES™ [Re: ouuwee]
#26683614 - 05/20/20 03:32 PM (3 years, 8 months ago) |
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Yes I’ve done it twice. 30 mins 15 psi. No warping so far and I ordered another pack.
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Astoriensis
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Re: EAT LIQUID INOCULATES™ [Re: ouuwee]
#26684102 - 05/20/20 07:20 PM (3 years, 8 months ago) |
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I got from Walmart the Mainstay brand of plastic lids. Half the price of Ball brand and work great so far. I don't even drill holes in them with rtv filters like before and they work just fine. They don't seal like metal lids used for canning so fae is provided.
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tedoro
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I've oddly has some of my fastest times with less LI too. There is something to that.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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eatyualive
Eat's You Alive :)



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Re: EAT LIQUID INOCULATES™ [Re: tedoro]
#26691479 - 05/23/20 11:53 PM (3 years, 8 months ago) |
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I’m loving these new lids. I already bought two more that I received today and used today. I’ve used them 4 times and no melting at 15 psi for 30 mins.
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Drboomer
The lord magnificent



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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#26804440 - 07/04/20 11:57 PM (3 years, 6 months ago) |
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Was there something you didn't like about the stainless pour lids? I bought a pair to try. Where did you get your new ones?
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PantsR4squares
Adventurer


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Re: EAT LIQUID INOCULATES™ [Re: Drboomer]
#27054720 - 11/23/20 10:40 PM (3 years, 2 months ago) |
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These are the lids he bought
https://www.masontops.com/products/multi-tops-easy-sip-pour-mason-jar-lids
I'm also curious why he prefers these over the stainless steel pour spout
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eatyualive
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They pour better and close between pours. The metal ones get stuck in up position sometimes. Its all a matter of technique but the plastic lid has more even pours due to width of hole. This can fuck you up if you over pour. I flick sideways for a split second and its the perfect amount about a teaspoon of inoculant per jar.
I do like that the metal one closes most of the time between pours. So its a different technique with no finger required to close between pours. I think you just have to pour a bit longer because the spout is not as wide. Definitely easier to regulate when first trying.
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RhYzo
D1g1t@l Dw3ll3r



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Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
#27098504 - 12/19/20 10:36 PM (3 years, 1 month ago) |
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Thank you for the write up!
Assembled my blades and waiting for it to cure before I clean them up and give em a try!
I forgot to score the blender base where the gasket meets, I'm thinking it will hold up though.
Last week I was about to toss out my 1990's ancient Oster blender because I broke the pitcher to it. And it's this awful un-godly baby shit pea green color lol I also kind of wanted a new one just to collect dust.
Now I found a way to repurpose this old thang!
Also really excited to give the LI a try for the first time. Thanks again eats!
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RhYzo
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Re: EAT LIQUID INOCULATES™ [Re: RhYzo]
#27099865 - 12/20/20 07:31 PM (3 years, 1 month ago) |
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Were you able to use the black base for support?
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TedsDead



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Re: EAT LIQUID INOCULATES™ [Re: RhYzo]
#27100162 - 12/21/20 12:02 AM (3 years, 1 month ago) |
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did you sterilize that base? mine melted immediately
-------------------- weed gets you through times of no money better than money gets you through times of no weed... -the fabulous furry freak bros If you can buy it, you can burn it!
https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396
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RhYzo
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Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
#27100170 - 12/21/20 12:12 AM (3 years, 1 month ago) |
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I didn't if you're asking me, I read through the thread and saw that it melted. Sorry that happened to ya! I just re-shared eats picture for reference, I should have quoted it.
Also from the images and assembly it kind of looks like the black base was used as an early proto type or example for pictures. I don't think there's no way to use them both without some modification. I could be wrong though! Usually am lol
I was thinking of cutting down the base just enough to give the blades a better support and a 'lift' away from the spinning mechanism.
Edited by RhYzo (12/21/20 12:14 AM)
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