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Invisibleeatyualive
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EAT LIQUID INOCULATES™ * 6
    #26337900 - 11/22/19 10:31 AM (4 years, 2 months ago)

:chief: EAT LIQUID INOCULATES :chief:
A Step By Step Guide




Brought to you by THE TRIBE


Hello Shroomery!  :hi:

:vibin:



This is how I liquid inoculate my quart grain jars. This tek is also assuming you know your SAB , Shmuvbox or Flowhood technique. In principle you can use any of these but the movements and open air exposure may vary. We will illustrate how it is done with a SAB. Some of the images show a Schmuvbox just for illustration purposes. We are focusing on the technique of pouring the liquid inoculant into receiving quart jars easily and evenly with a custom pour lid. You may use a flow hood or SAB for the method just make sure you stick to the principles of the method you prefer.
You can also do blenderless liquid inoculate but I like using a blender and a nice pour lid. The distribution is near perfect with a flick of the wrist and the jars generally colonize in 7-10 days without having to shake afte your initial inoculation and shake.

This recipe is 1 plate to 10 quart grain jars. You have the potential to stretch this to 20 quarts per plate but your colonization time may be slower than what is shown.


Supplies for blender attachment and pour lid build:

  • high temperature RTV silicone in a can



  • half pint tall regular mouth mason jar with metal band



  • half pint regular mouth mason jar pour lid that fits a straw



  • plug for pour lid. I use the blue injection ports shown below as a plug to close the pour lid.



  • Oster Brand blender blade assembly and grey gasket



    http://www.goodmans.net/i/2589/ice-crusher-blender-blade-cutter-for-oster-osteriz.htm

  • medium grade aluminum foil



    OPTIONAL POUR LID IF YOU DONT WANNA BUILD ONE

    1) This is autocleaveable but you don’t want to go past 15 psi for 30 minutes. I find this to be the best pour lid hands down. It snaps closed between pours and gives a nice even pour.



    https://www.masontops.com/collections/tough-tops/products/multi-tops-easy-sip-pour-mason-jar-lids

    2) This is available on amazon. It is stainless steel and fits regular mouth mason jars. It is intended for use as an oil and vinegar type pour for viscus liquids.



    https://www.amazon.com/Blulu-Pieces-Spout-Dispenser-Compatible/dp/B07YDQPVKR/ref=pd_aw_sbs_79_1/144-0245057-7019743?_encoding=UTF8&pd_rd_i=B07YDQPVKR&pd_rd_r=7aa1a725-3c8a-4319-ad72-2e893ec39caa&pd_rd_w=TeXbT&pd_rd_wg=x6abl&pf_rd_p=5c6e5bde-0dc6-44bf-853b-26c318f4dfa4&pf_rd_r=P2P0JWR6EAY8ZJKCC6JN&psc=1&refRID=P2P0JWR6EAY8ZJKCC6JN

    We tested the pour lid two ways with 1/4 pint of water in a regular mouth half pint.

    First one is the normal setting where the spout comes down about 1/4 inch below the bottom of the lid. This is how the lid is intended for use with oil. I’m assuming you want a delay with oil so a large amount doesnt come gushing all over the place. I also can’t get the remainder of the liquid out at the bottom of the batch and I feel it’s wasteful.



    The 2nd way we put the spout flush with the bottom of the lid. This seems to pour a little better and more controlled. I’m not sure I like the cap it’s a little funky. But it does provide a nice cover between pours if you need to take a break and it’s automatic. You could use your gloved finger to push it closed as well. I want a quick  motion where I flick once and it lets out as much inoculant as I need per jar. So what I’ve done in the image below is sit the spout flush with the bottom of the lid.



    You can see how with the spout flush, the water comes out in one quick spurt. This is how it works with the flush pour lid. This is ideal.



    SUPPLIES FOR INOCULATION

    • mostly colonized pasty plate of your favorite or best clean culture that is not touching the edge of the plate.



    • 70% isopropyl alcohol


    • spray bottle with a fine mist


    • SAB, Flowhood, Shmuvbox



    • Prepared grain jars of your choice. Oats are shown in this image


    • oster blender base


      This one is the cheapest on this site but any will work.

      http://www.goodmans.net/i/4697/oster-6629-bk0-10-speed-slope-blender-black.htm






    Procedure for building the pour lid


    1) take the band of your regular mouth tall half pint jar and apply a thin bead of RTV silicone to the inside ring.



    2) Add a small bead of silicone to the small suction hole that is built into the cup.



    3) Let the silicone set for about 10 minutes. Then Press the lid against the silicone. After that use your finger to seal the silicone on the inside and outside of the lid. Use the tip of your pointing finger to steadily push the silicone into a flat position and work your way all around the lid. A smooth steady motion with the finger will make a cleaner seal than my poor workmanship shown in the image. But this still works perfectly. There are a number of videos on youtube on how to apply a good seal by finger for silicone.







    4) The blue Injection port fits perfectly as a plug. Simply insert it and you are done. Let the silicone cure 24 hours prior to use. I also test the jar with liquid and see if it leaks prior to use. Sometimes you apply too much silicone and you can simply cut it down with a sharp knife or completely reapply if necessary. It may take a few trials to get your silicone application perfect. There is an art form to it. This is the pour lid complete.




    Procedure for building the blade assembly


    1) Take your regular mouth band and place a silicone bead around the edge of the inside of the band.



    2) Wait 10 minutes and then press the metal blade assembly into the band.

    3) Now place the grey gasket down over the silicone and press down to allow the gasket to set in. The gasket is necessary so that the jar seals properly when you are blending the jar. Otherwise it will explode or leak out of the jar during blending.



    4) Use your finger to seal the silicone in place as mentioned above. Seal the inside and outside of the blender assembly.





    5) Let the silicone cure 24 hours prior to use.  I also test the blender assembly prior to use to make sure it fits the half pint mason jar and does not leak. I fill it with water and blend a few blends.  If it leaks you may need to redo your silicone application or cut back some silicone if you applied too much.


    Procedure for liquid inoculation


    Recipe: One regular mouth 1/2 pint was filled half full with tap water (1/4 pint water to 1 Pasty plate).

    1) Fill a half pint jar half full with tap water. The blade assembly is placed on top of the half pint jar and screwed on. I then place a piece of medium grade foil to cover the entire lid.



    2) The pour lid is wrapped in foil. I use roughly the length of a medium grade foil roll by about 4 inches wide. I fold it down the middle. Take note, I place the open part of the lid down on the foil and wrap the foil over it.



    I fold (3) 1/4” wide  flaps on 3 sides and fold them over. This comes in handy when you unwrap the foil to place the lid on the mason jar for pouring. In addition, you can recycle the foil and use it anytime you cook the lid. I’ve used the same foil for about 3 plus years until it’s worn out and needs replacing.



    This is how the lid is wrapped.



    This is how the lid is unwrapped


    The reason is you can unfold the sides without exposing the bottom portion of the pour lid before making your lid swap onto the blended mason jar for distribution.
    Both items were pressure cooked for 20 minutes at 15psi. I lump these pour lid and assembly items when I make pasty plates so you are not doing any extra pressure cooking. It can all be done at one time.

    3)Once the pressure cooker is cool.  Prepare your SAB for work. Place all utensils in your SAB. Foil covered pour lid, isopropyl soaked paper towel, colonized pasty plate, 1/2 pint mason jar with blade assembly topped with foil.

    4) Plugin your Oster blender base near your work area. I use gloves and wipe down my hands up to my elbows with 70% iso before placing them in the SAB.

    5) Now it is time to Tiger Drop your plate into the blender jar. Wipe the outside of your pasty plate and blender jar with the iso paper towel.

    Take your finger and thumb and loosen the culture/agar from the bottom of the pasty plate. Remove the foil from the blender jar. Loosen the lids on both the plate and blender jar. I use my left hand to hold the pasty plate and my right to lift the lid of the blender jar.



    I simultaneously open the blender jar and plate and drop the entire culture directly into the blender jar in one plop. The inside of blender blade lid  is directly above the blender jar as you make the drop so when the blender jar lid is opened the lid acts as a barrier from things falling from above. This is one steady motion and the exposure of both the culture and the blender jar is milliseconds



    Below is an illustration of how you would do this with a normal plate or holy grail plate. I simply flamed my exacto knife and cut around the culture. Then cut the culture into 4 equal pie pieces. I then drop the plate flipping it upside down into the blender assembly jar.


    6) Screw the lid of the blender jar on tight. The jar is then taken out of SAB and placed on the oster blender base. The culture is blended on the oster blender unit for 3-5 seconds or until the entire plate and culture a puréed into a fine texture. Wipe down the outside of the blender jar with an iso paper towel.  Place the blender jar back into the SAB.

    The image below is an example of a 1/2 pint jar being blended on an oster blender. This is for reference to show you what it looks like.



    This is what your liquid inoculate will look like after the blend. The mycelia pieces will settle to the bottom when you leave the jar alone.



    7) Before you bring the jar back into the SAB make sure to wipe it down with an iso paper towel. Now it is time to swap your blender assembly for the pour lid. Loosen the lid of your blender jar so the swap is quick and easy. You loosen it enough to where you can lift the lid for the swap but leave the lid on top of the jar until the swap.





    8) Below you can see how the foil is unfolded on two sides but left in place until the swap.




    With my right hand, I lift the pour lid and with my left hand I lift the blender jar lid. I swap them at the exact same time so exposure is minimal. Now your jars are ready to inoculate. This is a quick time lapse of the lid swap.(im using my new stainless steel LI pour lids see above.)





    9) I then load my grain jars into the SAB. I wipe each one down all over with an iso paper towel before it is placed into the SAB. I loosen each lid by hand. I let the air settle prior to doing any work.





    12) Remove the plug before the pour. I Lightly swirl the liquid inoculate jar before each pour as the mycelia slurry settles. With my left hand I lift the grain jar lid, with my right hand I do a quick turn for a split second and then close the grain jar lid. While I do all this, the grain jar lid is held directly above the grain jar opening to act as a shield from anything falling from above. Grain exposure is minimal during the pour and basically the pour is done underneath the grain jar lid. With these new lids the spout has a small latch that closes between each pour for convenience. There is about 1cc volume distributed into each jar from the quick  flick of the wrist.

    Swirl technique and loosening the lid

    Inoculation


    13) As i am putting the jar down between each pour, im using my other hand to screw the lid of receiving jar back on. Tighten each lid. You can now remove the jars from the SAB to shake them.

    14) Shake each jar thoroughly. The idea is that you shake and turn the jar as you are shaking. I almost do an upward motion while shaking so it folds the grain from bottom to top over the liquid inoculate. What this does is distribute all of the liquid inoculate evenly. The key to not having to shake later on is to spend the time shaking up front. I spend about one minute per jar. After that, no shaking necessary.

    You can see a video below on pouring the liquid inoculant in a SAB by Carcase-x to illustrate the pour.



    Below are some images of how it looks after inoculation. The jars were not shaken once the initial shake was complete.

    Day 4 after inoculation


    Day 7 after inoculation



    The beauty of liquid inoculation is that if you shake properly right after inoculation you do not need to shake again. This saves you time and man power in the long run. Generally you will have colonized jars in 7-10 days and you are ready to spawn. I like the fact that all I have to do after inoculation is spawn. No shake necessary.



    Here are a few sample grows using LI. Syzygy is shown below.



    AA+



    LGT



    Tex



    After your jars colonize it is time to prepare your bulk substrate.

    EATS UNBUCK3T TEK


  • Edited by eatyualive (05/26/20 12:19 PM)


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    InvisiblefahtsterM
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    Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
        #26356248 - 12/01/19 08:50 PM (4 years, 2 months ago)

    This thread was moved from the user's journal.

    Reason:
    Eats request


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    Invisibleeatyualive
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    Re: EAT LIQUID INOCULATES™ (moved) [Re: fahtster]
        #26415747 - 01/04/20 09:35 AM (4 years, 1 month ago)

    I’ve recently switched to the holy grail plates. I feel it’s a completely different technique as far as tiger drop. I did one batch yesterday and simply loosened the plate with a bernzomatic torch flame sterilized exacto knife and dropped it. While doing this I used the lid of the dish at a 45 degree angle to shield anything from above while I loosened the plate with the exacto.

    I prefer the pasty plate because I can loosen it prior to opening and exposing the culture to the SAB. Then do a quick drop with minimal exposure during drop. However, what I may do is do my Initial transfers on the holy grail plates and do my 2Nd and 3rd transfer on pasty’s for ease of technique using a tiger drop.

    I’ve also simply used a normal plate and cut 1/3 of the plate and dropped it in as well. It all works and you could use an entire plate but the pasty plate is well suited for the drop into the tall half pint due to size and technique.

    It definitely was not as smooth as the pasty plate. But you give and take easy tiger drop for better visibility. The holy grail plates are also too hard to loosen the agar from the plate


    --------------------
    EAT GETS SHIT DONE


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    OfflineAstoriensis
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    Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
        #26415855 - 01/04/20 11:05 AM (4 years, 1 month ago)

    Cool write up here:greenthumb::vato:

    I've been thinking of something similar to this but an all in one blender blade and injection port. Perhaps there is no room looking at your pix...

    That way no messing with removing lids.


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    OfflineFunnyFungiName
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    Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
        #26415860 - 01/04/20 11:13 AM (4 years, 1 month ago)

    Awesome write up! Have you tried larger jars like a pint or used 1 culture to 5 instead of 10?


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    Invisibleeatyualive
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    Re: EAT LIQUID INOCULATES™ (moved) [Re: FunnyFungiName]
        #26415926 - 01/04/20 11:57 AM (4 years, 1 month ago)


    Quote:

    I've been thinking of something similar to this but an all in one blender blade and injection port. Perhaps there is no room looking at your pix...




    We did this thing called a tribal container years ago. It had a blender and pour lid all in one. I was using it for a while but the bottom metal blade assembly are difficult to adhere to the plastic. The plastic and metal don’t like to stick together much so to say.

    https://www.shroomery.org/forums/showflat.php/Number/21927954

    Quote:

    Have you tried larger jars like a pint or used 1 culture to 5 instead of 10?




    Yes I’ve tried regular mouth pints, quarts ect. But they have to regular mouth. You can also buy the pour lid in wide mouth version but the blade assemblies then have to be attached to the lid and it is more difficult to do so.

    My ratio is for 10 quarts. But I’ve done 20-30 quarts with one half pint. You could use a pint then you would have 1/2 pint water for inoculant. You can double up your quarts in that case. You don’t want to overpour though so the grain doesn’t get water logged with pooling water. After inoculation and shake you shouldn’t visibly see any pooling water. The inoculate water should be distributed evenly throughout the jar.







    --------------------
    EAT GETS SHIT DONE


    :flame::chief:JOIN THE POW WOW:chief::flame:


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    Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
        #26415947 - 01/04/20 12:13 PM (4 years, 1 month ago)

    Quote:

    eatyualive said:


    We did this thing called a tribal container years ago. It had a blender and pour lid all in one. I was using it for a while but the bottom metal blade assembly are difficult to adhere to the plastic. The plastic and metal don’t like to stick together much so to say.







    yeah, I still have a few of those.  I really like the concept but the blades definitely dont like to stay attached:facepalm3:    I cant say ive been doing too much blending lately but theres a nice 1 piece pour lid with a gasket by "masontops" . works great for the pour without having to mod.  only thing is although it claims PP5  they warp @ 20 psi  so u gotta keep it at 15 if u use em . theyre like 5-8 bucks a piece so it hurts when they break


    how snug does your rubber port fit into the hole in the lid eats?    I have a couple that I may have sanded a bit too much.  no good for the tribal container but may still work for this application


    --------------------
    weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
    If you can buy it, you can burn it!



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    Invisibleeatyualive
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    Re: EAT LIQUID INOCULATES™ (moved) [Re: TedsDead]
        #26416375 - 01/04/20 05:28 PM (4 years, 1 month ago)

    Do you have a link to it? I’ll try it for sure! Rubber plug is very tight actually.


    Look these are metal or stainless steel. No more building required. I’ll update the op when I test these. They look convenient between pours.

    https://www.amazon.com/Infusions-Liquor-Closed-Dispenser-Regular/dp/B07LF5GYC5/ref=pd_aw_sim_79_8?_encoding=UTF8&pd_rd_i=B07LF5GYC5&pd_rd_r=793bf21d-c6c7-494b-8e9e-5cb8e9666e5d&pd_rd_w=gp4Wy&pd_rd_wg=IkweC&pf_rd_p=a966b482-aeee-4b16-9ffe-9a1cb857c30c&pf_rd_r=WZF9M3JM5KV1WM4TK5S0&psc=1&refRID=T33Y5ZE34D9FKPJH054G


    I ordered 2. This is money!




    Crap, look at the image of the bottom. That’s going to be an issue with clogging and stopping the blended up mycelial pieces. Fuck it I’ll still try.


    --------------------
    EAT GETS SHIT DONE


    :flame::chief:JOIN THE POW WOW:chief::flame:


    Edited by eatyualive (01/04/20 06:15 PM)


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    Invisibleeatyualive
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    Re: EAT LIQUID INOCULATES™ (moved) [Re: eatyualive]
        #26416481 - 01/04/20 06:25 PM (4 years, 1 month ago)



    --------------------
    EAT GETS SHIT DONE


    :flame::chief:JOIN THE POW WOW:chief::flame:


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    Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
        #26416506 - 01/04/20 06:42 PM (4 years, 1 month ago)

    😎🍿


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    Invisibleeatyualive
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    Re: EAT LIQUID INOCULATES™ [Re: YogiBear]
        #26416525 - 01/04/20 06:55 PM (4 years, 1 month ago)

    So found a user image on the first one. It appears this may work. The smaller line appears to be an air intake. Which may or may not be an issue. I’d use the plastic lids with caps but I definitely don’t want to melt them in the cooker.



    --------------------
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    :flame::chief:JOIN THE POW WOW:chief::flame:


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    Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
        #26416531 - 01/04/20 07:02 PM (4 years, 1 month ago)

    Damn I read the title and thought I was gonna get to see some fucktard chugging a jug of lc haha.

    But actually I was just starting to read up on LC/LI so I'm glad I ran across it thanks for write up eat.


    Edited by Hillbillyjohn (01/04/20 07:05 PM)


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    InvisiblePastywhyteMDiscord
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    Re: EAT LIQUID INOCULATES™ [Re: Hillbillyjohn]
        #26416759 - 01/04/20 10:03 PM (4 years, 1 month ago)

    Love that you’re still rocking the blender mods. I did have some success myself with them a few years back. Nice stuff man!


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    Invisibleeatyualive
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    Re: EAT LIQUID INOCULATES™ [Re: Pastywhyte]
        #26416766 - 01/04/20 10:07 PM (4 years, 1 month ago)

    Wish they had that lid 5 years ago :facepalm:


    --------------------
    EAT GETS SHIT DONE


    :flame::chief:JOIN THE POW WOW:chief::flame:


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    Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
        #26416827 - 01/04/20 10:57 PM (4 years, 1 month ago)



    --------------------
    weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
    If you can buy it, you can burn it!



    https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396


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    Invisibleeatyualive
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    Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
        #26416837 - 01/04/20 11:07 PM (4 years, 1 month ago)

    I was looking at those initially. Very similar to the blender ball lids I’ve used. I’ve pressure cooker the blender balls with no issue. And I was actually thinking of trying those if the stainless steel don’t work. I definitely don’t want to melt them. I’m also concerned with the stainless steel assembly that the seal may melt. It doesn’t on the oster blender blade gasket but I guess we won’t know until it is tested. I’ll be the guinea pig.


    --------------------
    EAT GETS SHIT DONE


    :flame::chief:JOIN THE POW WOW:chief::flame:


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    Re: EAT LIQUID INOCULATES™ [Re: eatyualive]
        #26417174 - 01/05/20 08:47 AM (4 years, 1 month ago)

    lol!

    well,  I only melted one once when I let my pc get over 20psi on the stove top.  the metal ones look nice but the flow rate seems like it not be enough to pour quickly.  if your attentive and watch your pc and dont get too stoned:facepalm3:    there shouldnt be any issues with melting.  the one tht I "melted" . just warped a bit and looks ugly but is still fully functional.  fack tho that website lists a higher retail than I paid at the store!?


    --------------------
    weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
    If you can buy it, you can burn it!



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    Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
        #26417188 - 01/05/20 09:02 AM (4 years, 1 month ago)

    So I just realized my magic bullet blender is pp5, I did a quick search and found some people who melted theirs in the PC but no mention if it was pp5 plastic. Anyone have recent experience using these?


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    Re: EAT LIQUID INOCULATES™ [Re: StrohngBahd]
        #26417192 - 01/05/20 09:07 AM (4 years, 1 month ago)

    theyll melt.  pretty sure theyre PET or PTF.  says right on the magic bullet "not for hot liquids"  so I def wouldnt fuck with it in a PC.  you can tell at a glance that it doesnt even look like PP5 plastic


    --------------------
    weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
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    Re: EAT LIQUID INOCULATES™ [Re: TedsDead]
        #26417251 - 01/05/20 09:54 AM (4 years, 1 month ago)

    Anyone ever try it with a Ninja blender. I feel like its made of some bad ass plastic. I just use it to grind up weed and mush for chocolates.


    Edited by Bph (01/05/20 09:56 AM)


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