Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Cultivation Supplies   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Substrate Bags   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
InvisibleBigSurMoon
the deathless ones
 User Gallery


Registered: 03/21/18
Posts: 876
Loc: Chicken coop
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: bongoman]
    #26336647 - 11/21/19 06:22 PM (4 years, 2 months ago)

Quote:

bongoman said:
Quote:

verum subsequentis said:
Did any of the new plates, that haven't been nocced up yet, show any contams?




Bingo. You'd think I would have checked that already but for some reason I'd just assumed that my new plates were clean as I've never had problems with my agar process previously.

9 out of 10 plates that were made at the same time but not inoculated are showing the mold.

So it's my plate making process that is suspect. I do no pour plates and can only think I didn't sterilise long enough as they were never opened after sterilising.




May also want to see how well the lids fit, after a while they can not fit so snug, I have had that be an issue.


--------------------
My soul brings tears, to angelic eyes.


DISCOVER BLACK KOW
I swear that "black kow experiment" thread is gonna get it. It's tempting me to adress it. Sounds both pornografic and racist...
https://www.shroomery.org/forums/showflat.php/Number/25300352/page/1




Deliberately asleep, always.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: BigSurMoon]
    #26336732 - 11/21/19 07:03 PM (4 years, 2 months ago)

Wouldn't be a sterilizing issue molds are killed very easily I would look at your filters


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinebongoman
Stranger
Male User Gallery
Registered: 09/19/18
Posts: 558
Last seen: 24 days, 2 hours
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: cronicr]
    #26336765 - 11/21/19 07:18 PM (4 years, 2 months ago)

Quote:

cronicr said:
Wouldn't be a sterilizing issue molds are killed very easily I would look at your filters





I have a variety of different no pour containers, some with filters, others are sterilised with loose screw-top lids and then tightened.


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: bongoman]
    #26336767 - 11/21/19 07:20 PM (4 years, 2 months ago)

Molds die off at around 140 so even pasturizing kills them.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Offlinebongoman
Stranger
Male User Gallery
Registered: 09/19/18
Posts: 558
Last seen: 24 days, 2 hours
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: cronicr]
    #26336968 - 11/21/19 08:46 PM (4 years, 2 months ago)

Quote:

cronicr said:
Molds die off at around 140 so even pasturizing kills them.




Thanks for that info. All I can try is another batch of plates I guess and maybe ensure they are cooled in a clean place.


Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,865
Last seen: 17 hours, 21 minutes
Trusted Cultivator
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: bongoman]
    #26336984 - 11/21/19 08:54 PM (4 years, 2 months ago)

What exactly are you seeing? Is it only mold, no bacteria, and all the same kind of mold? Are there little trails like something crawled over it? Mold colonies are usually caused by dust particles from the air. It's kind of strange you'd be getting it in different kinds of unopened dishes.


--------------------


Extras: Filter Print Post Top
Offlinebongoman
Stranger
Male User Gallery
Registered: 09/19/18
Posts: 558
Last seen: 24 days, 2 hours
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: Kizzle]
    #26336988 - 11/21/19 08:56 PM (4 years, 2 months ago)

Random spots of dark color, no trails. All the same kind. I'm totally puzzled by it.


Extras: Filter Print Post Top
Offlinecurious.psychonaut
Stranger
I'm a teapot User Gallery


Registered: 10/17/19
Posts: 282
Last seen: 4 years, 28 days
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: bongoman]
    #26337050 - 11/21/19 09:36 PM (4 years, 2 months ago)

Quote:

bongoman said:
All I can try is another batch of plates I guess and maybe ensure they are cooled in a clean place.




If they need to cool in clean places, something's wrong, because cool places are not clean (without equipment/FH/etc.) and you probably intend to store the plates normally anyway, right?

Since the problem occurs post-PC, it's either in the procedure or in the containers. You said you use a variety of containers, so it's probably procedure. Do you: wrap plates in foil for PC? Do anything to the plates after PC, except tightening caps before taking them out of the PC (and before removing the foil)?


--------------------
My LAGM2020 grow log



Extras: Filter Print Post Top
Offlinebongoman
Stranger
Male User Gallery
Registered: 09/19/18
Posts: 558
Last seen: 24 days, 2 hours
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: curious.psychonaut]
    #26337074 - 11/21/19 09:50 PM (4 years, 2 months ago)

I don’t wrap plates in foil and as you mention, only tighten lids if they are screw top.

Here’s a photo, the 2 plates on the right are not inoculated and haven’t been opened since PC’ing.



Extras: Filter Print Post Top
OfflineJohnRainy
Stranger

Registered: 07/09/19
Posts: 1,244
Last seen: 4 years, 15 days
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: bongoman]
    #26337086 - 11/21/19 09:59 PM (4 years, 2 months ago)

That's fucked up. 

And it worked before.

Could those containers be losing their integrity?  It's hard to inadequately sterilize agar.


Extras: Filter Print Post Top
InvisibleZifozonke
Stranger
 User Gallery

Registered: 03/24/19
Posts: 1,258
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: JohnRainy]
    #26337141 - 11/21/19 10:28 PM (4 years, 2 months ago)

...i got deja vu looking at your dishes ....
Almost exactly what I was getting..

Think mine are worse thou...


Extras: Filter Print Post Top
Offlinecurious.psychonaut
Stranger
I'm a teapot User Gallery


Registered: 10/17/19
Posts: 282
Last seen: 4 years, 28 days
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: bongoman]
    #26337168 - 11/21/19 10:48 PM (4 years, 2 months ago)

Yeah, can't blame it solely on the filters because you also have screwtops. Unless there is an integrity problem with the screwtop containers, the contam seems to have happened inside the PC, right? After that, they are airtight?

Theory: as the PC was cooling off (steam condensing=negative pressure), it sucked in spore-laden air through the valve. The filters didn't work (b/c bad? wet?); the loosened lids didn't provide enough of a barrier.

So why not wrap half the containers snugly in foil and see if it makes a difference? (Tighten lids before removing the foil. Dry filters in the SAB, removing foil once in the SAB.)


--------------------
My LAGM2020 grow log



Edited by curious.psychonaut (11/21/19 11:03 PM)


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: curious.psychonaut]
    #26337181 - 11/21/19 10:55 PM (4 years, 2 months ago)

Or just wrap your lids with micropore tape...or get different dishes


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 1 day, 4 hours
Trusted Cultivator
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: cronicr]
    #26337251 - 11/21/19 11:32 PM (4 years, 2 months ago)

Time for some petris perhaps?


Extras: Filter Print Post Top
Offlinebongoman
Stranger
Male User Gallery
Registered: 09/19/18
Posts: 558
Last seen: 24 days, 2 hours
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: Failboat]
    #26337255 - 11/21/19 11:34 PM (4 years, 2 months ago)

Quote:

Quirkmeister92 said:
Time for some petris perhaps?




I’ve never been closer to investing in a laminar flow hood and doing pour agar.


Extras: Filter Print Post Top
OfflineFailboat
Fuck Up

Registered: 02/01/18
Posts: 8,736
Last seen: 1 day, 4 hours
Trusted Cultivator
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: bongoman]
    #26337261 - 11/21/19 11:37 PM (4 years, 2 months ago)

You can pour petris easily and reliably in a SAB while you sort out your FH. Gonna go to the hardware store tomorrow and pick up wood and wiring for my 2x4 build. First I'm gonna pour some petris for breakfast.:rasta:


Extras: Filter Print Post Top
InvisibleBph
Stranger
 User Gallery


Registered: 10/11/18
Posts: 1,466
Re: Mold on 95% of plates after an SAB transfer session. WTF??!! [Re: Failboat]
    #26337581 - 11/22/19 08:01 AM (4 years, 2 months ago)

Quote:

Quirkmeister92 said:
Time for some petris perhaps?




Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: North Spore Cultivation Supplies   PhytoExtractum Kratom Powder for Sale   Mushroom-Hut Substrate Bags   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Re: mold in agar plate Anonymous 12,256 3 12/04/99 12:35 AM
by Anonymous
* Re: Green mold again! Agar experts help me! Anonymous 7,380 9 12/01/99 10:20 AM
by Anonymous
* How to make agar 2 agar transfer? esp 6,676 7 01/08/04 11:08 AM
by YidakiMan
* agar transfers *DELETED* thisone 1,246 3 11/20/03 08:58 PM
by Mycomancer
* G2G transfers...an endless cycle? ADoobie 7,207 18 11/12/20 07:10 PM
by FredsGotSlacks
* Is this ready to transfer??? Junkaboy 657 2 03/16/05 01:18 PM
by Olgualion
* agar to agar transfer ohmatic 15,678 11 01/12/21 09:00 PM
by bodhisatta
* transferring "normal" to "peroxide agar" - dunk the tissue in h2o2 ? ohmatic 2,858 12 05/25/05 02:19 PM
by ohmatic

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
951 topic views. 31 members, 130 guests and 53 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.056 seconds spending 0.007 seconds on 13 queries.