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OfflineACEEE
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Registered: 11/20/19
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Should I be worried(rye jars)
    #26333213 - 11/20/19 10:36 AM (4 years, 2 months ago)

Bought organic grain, rinsed grain, boiled grain 10 min, soaked grain with gypsum, loaded 1\10 of the grain each jar into 10 jars (about 1.5 cups with the added water) Used instant pot instead of pc (110 min instead of 90) about 3 days later I loaded into jars (beautiful looking grain) knocked her up with 1cc spore from a trusted vendor per jar(a little low, I know) covered hole with poly fill and shook. Tomorrow will be one week since inoculation and I have no myc growth. Should I be worried? What crucial mistakes did I make?m

Edit: jars are being kept room temp
Edit #2: I wrote down my procedures wrong. By bad. I’m forgetful. I have a log I posted below though.


Edited by ACEEE (11/20/19 11:56 AM)


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Offlinefeldman114
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Re: Should I be worried(rye jars) [Re: ACEEE]
    #26333266 - 11/20/19 11:01 AM (4 years, 2 months ago)

Quote:

ACEEE said:
Bought organic grain, rinsed grain, boiled grain 10 min, soaked grain with gypsum, loaded 1.5 cups each jar into 10 jars, added water, Used instant pot instead of pc (110 min instead of 90) about 3 days later I loaded into jars (beautiful looking grain) knocked her up with 1cc spore from a trusted vendor per jar(a little low, I know) covered hole with poly fill and shook. Tomorrow will be one week since inoculation and I have no myc growth. Should I be worried? What crucial mistakes did I make?m

Edit: jars are being kept room temp




No offense but idk where to begin. You seem to have taken bits and pieces of different grain prep teks and mashed them together.

Most importantly, 1. sounds like you had an open hole in your jars, that you stuffed with poly AFTER inoculation? Should be before PC. 2. Instapots may be ok for half-pint jars but not quarts. Maybe try pint jars with 3-4 hours in the instapot...I PC my grain at 14 psi for 2 hours and barely get it sterilized.

Can you explain your grain prep step by step? Especially the part about waiting 3 days before loading jars?

Also, around here pics=replies 😉


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OfflineACEEE
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Re: Should I be worried(rye jars) [Re: feldman114]
    #26333354 - 11/20/19 11:43 AM (4 years, 2 months ago)

Yeah, I followed a grain tek I saw on another site. I’m new here, and I’m sure you guys do things differently. With that said, this was a mess of a second attempt. (As I live with parents, I did this at my friends house and didn’t end up having the access I thought I would)I made a lot of mistakes. I’d just like to know which ones are more critical so I can identify what the cause of my jars not working are(if they don’t). That was one problem I forgot to mention. I forgot to poke the hole before I pc’d them!! I read that this could cause contams, but I didn’t know if this would be a fatal mistake worth starting over for. The tek I looked at said 90 min pc at 15 psi for 90 mins. As my instant pot caps out at around 11-13 pci, I read that I would need to increase time by about 15%. Honestly, I think this was too low and I should have pcd for 2 hrs. I did not know insta pot wouldn’t work for quarts. Truthfully, before I started I thought I had a pc, but turns out it was an instant pot. I’ll be looking to buy a pc in that case. About the three day’s... that wasn’t part of the tek! I meant to include that as a mistake I made. I didn’t have access to my friends house for a few days. I didn’t think this would be a fatal mistake, since the jars usually sit out awhile after inoculation anyways.

If you would like me to link my tek I followed I can do that, and I can send pictures either later today or tomorrow.


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OfflineACEEE
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Re: Should I be worried(rye jars) [Re: ACEEE]
    #26333368 - 11/20/19 11:51 AM (4 years, 2 months ago)

Here are the general notes I wrote down from the tek. So these are what I actually followed.
Edit: I originally posted 1.5 cups per jar. It was 10 cups total for 10 jars, one each. It came out to about 1.5 cups per jar with added water from soak and boil. I did NOT add water to jars after preparing grain. My bad!
10 cups rye
Rinse three times
2500 ml water
1 tbl gypsum
Soak 24 hr
Boil 10 min
Drain
Load jars
Cover jars with tin foil
Pc 15 psi 90 min(instant pot steam 2 hrs)
Hole in jar lids
inoculate
Poly fill


Edited by ACEEE (11/20/19 11:58 AM)


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InvisibleTeaforTwo
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Re: Should I be worried(rye jars) [Re: ACEEE]
    #26333415 - 11/20/19 12:08 PM (4 years, 2 months ago)

1. welcome to shroomery!

2. add pictures so folks can see what it looks like.

3. what compelled you to rinse, boil, soak, then load in jars with additional water? if following advice or a 'tek' then do please share link to or explain of this in detail. also not understanding what you mean by " covered hole with poly fill and shook" after inoc with spores. you should have had GE filter (polyfill in this case) in place before you sterilized your grains and unless you unloaded your IP and inoculated in front of laminar flow hood it is very likely you contaminated all jars of grain by adding polyfill late in the game.

4. shooting spores direct into grain spawn is a big gamble that raises the more you squirt in due to competing spores and contaminants. It is uncommon in practice because it is less likely to produce like we want even if it does not contam out in the jar. learn agar is a mantra you will hear suggested often until you learn to sing the song of its benefits too.. you may not be seeing any growth at 1 week and this may be an indication of the good myc not winning the fight against contams present, could completely stall your jars or soon you'll see all sorts of questionable stuff growing, like bacterial creamy stuff and gross mold stuff.

5. unlikely you sterilized the grains in jars using an IP for that length of time involved. consider it a minimum, 90min at 15PSI (I add 15-20% time and PC at 21PSI for such recipes since one mile high elevation), this is in a pressure cooker which has been vented properly (minimum 10 minutes solid steam vent before close toggle or put on weight) before starting timer.

depending your model IP your cook time will vary in pressure from 9-10PSI, 10-12PSI or 13-14.5PSI and your time needs be adjusted based on the lower number of that PSI range to determine how much time needs be added since it is significantly less than standard of 15PSI and greater constant pressure/temp, IP are not a constant pressure cook, it varies a lot in that range so you have to use the minimum as your measure from point.

10PSI is 239F, 15PSI is 250F, 20PSI is 259F at sea level, the temp is what kills the bad stuff over a period of time, also consider how dense you pack the IP with jars and how full each jar is. at 10PSI i'd at a minimum double the 15PSI recipes time, so 180minutes when i saw 90minute suggested. i'm not good with math or the science of this so look for better suggestions on actual time to use for what you intend to sterilize. We have an IP and due to small size and questionable sterilizing ability we limit it to use for agar cook, 40min at sterilize function setting, versus 20min at 15PSI if we used a PC.

for what has already been done, wait and see what it looks like if proven inconclusive based on photos you upload and what others respond accordingly.


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Offlinefeldman114
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Re: Should I be worried(rye jars) [Re: ACEEE]
    #26333418 - 11/20/19 12:11 PM (4 years, 2 months ago)

Here’s how to prep grains:
https://www.shroomery.org/forums/showflat.php/Number/20048765#20048765

But yes, you can toss these jars. We PC to kill contams, which you introduced again when you made those holes. You gotta make the lids first.

How did you inoculate? Do u have a SAB? If not, look into using one.


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OfflineACEEE
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Re: Should I be worried(rye jars) [Re: TeaforTwo]
    #26333445 - 11/20/19 12:23 PM (4 years, 2 months ago)

1. Thank you!

2. Will do ASAP

3. I’m sorry for the confusion. I did not add additional water after boil and soak. I’ve edited my post. I posted my notes I took from the tek I was following above. I based my procedures off my notes. In the future I will be poking holes before pc. That was my mistake for sure.

4. Agar is on my list of next things to learn. I wanted some shroomies to hold me over first though.

5. In order to solve his problem, I will buy a PC with 15psi setting.

Thanks, I appreciate all you guys ❤️


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OfflineACEEE
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Re: Should I be worried(rye jars) [Re: feldman114]
    #26333456 - 11/20/19 12:30 PM (4 years, 2 months ago)

I will look into following this tek in the future, thank you.


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OfflineKizzle
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Re: Should I be worried(rye jars) [Re: ACEEE]
    #26333457 - 11/20/19 12:30 PM (4 years, 2 months ago)

Quote:

covered hole with poly fill and shook.



I'm not sure what you mean here. Did you poke the syringe through a taped hole or something? Or you just had an open hole? Because having a hole open for more than a second or two while you inoculate is likely to cause problems and while quickly covering an inoculation hole with tape may work, trying to cover it with polyfill or stick polyfill into it could contaminate it.

Quarts should be sterilized for 90 minutes at 15 psi. 90 minutes at 10 psi or slightly higher will probably mostly work but you're pushing the limit to where you'll start seeing bacterial grains.


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OfflinemeaculpaUIO
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Re: Should I be worried(rye jars) [Re: Kizzle]
    #26333485 - 11/20/19 12:42 PM (4 years, 2 months ago)

Pics


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OfflineACEEE
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Re: Should I be worried(rye jars) [Re: Kizzle]
    #26333522 - 11/20/19 12:57 PM (4 years, 2 months ago)

Okay, in the future I will use Micropore tape.

Do you think I could just stick with an instant pot then?


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OfflineACEEE
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Re: Should I be worried(rye jars) [Re: feldman114]
    #26333534 - 11/20/19 01:03 PM (4 years, 2 months ago)

Do you mind if I ask which monotub tek you use your spawn for?


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Offlinefeldman114
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Re: Should I be worried(rye jars) [Re: ACEEE]
    #26333541 - 11/20/19 01:08 PM (4 years, 2 months ago)

Quote:

ACEEE said:
Do you mind if I ask which monotub tek you use your spawn for?




This is how I make them:
https://www.shroomery.org/forums/showflat.php/Number/20356759
You can replace the polyfil with micropore tape.

But I do this only because I have cats, so I need to keep my tubs sealed at all times. Here’s what I’d do if I had a separate grow room:
https://www.shroomery.org/forums/showflat.php/Number/22337800


Edited by feldman114 (11/20/19 01:09 PM)


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OfflineKizzle
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Re: Should I be worried(rye jars) [Re: ACEEE]
    #26333549 - 11/20/19 01:10 PM (4 years, 2 months ago)

Quote:

ACEEE said:
Okay, in the future I will use Micropore tape.

Do you think I could just stick with an instant pot then?



Yes. Just increase the time to 120 minutes starting from when it reaches full pressure.


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