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Invisiblemurderlabz
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Registered: 05/18/19
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Optimization of culture conditions for Panaeolus cyanescens 2014
    #26332525 - 11/20/19 12:24 AM (4 years, 2 months ago)

Optimization of culture conditions for mycelial growth and basidiocarp production of Philippine strains of Panaeolus antillariumand Panaeolus cyanescens

Abstract
The  domestication  and  optimization  of  cultural  conditions  for  the  secondary  mycelial growth  was  determined  to  establish  the  production  technology  of Panaeolus species.  Wild P. antillarumand P.  cyanescenswere  collected  and  their  cell  lines  were  rescued.    The  mycelial growth performance was evaluated on different indigenous culture media (carabao, cow and horse dung)  and  physical  factors  (pH,  aeration  and  illumination).  The  optimal  growth  condition  for basidiocarp production using dung of domesticated ruminants was also determined. Both secondary mycelia  of  the  two Panaeolusspecies  evaluated  grew  best  on  solid  culture  media  of  carabao  and cow  dung  decoction gulaman  (local  crude  agar)  at  a  pH  range  of  7.5 -8.0,  incubated  in  unsealed and  dark  condition.  Carabao  and  cow  dung  as  substrates  favored  the  fast  mycelial  colonization  of both  mushrooms  with  means  of  11.20±  4.24and  11.50±  5.80days,  respectively. However, regardless of the substrate, P. antillarumcolonized the substrates in a shorter period of 9.37± 2.74days. In  terms  of  yield,  carabao  dung  had  the  highest  mean  yield  of  7.16 ±  0.75 g  with  17.89± 1.88% bio-efficiency, which did not significantly vary with cow dung having a mean yield of 6.99 ± 1.89 g with 17.49± 4.73% bio-efficiency. These significant results suggest thatP. antillarumand P. cyanescensare new Philippine strains of wild mushrooms with great potential for cultivation for nutraceutical purposes

Full Paper -> https://pdfs.semanticscholar.org/6981/a1e8b98bc6b0aa38d135fbd2340149d79811.pdf


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