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OfflineInthepit
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Registered: 08/20/19
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Syringe not shaken enough?
    #26311076 - 11/10/19 08:14 AM (1 year, 5 months ago)

I inoculated 10 plates with MS syringe, only 3 showed after 10 days.
It looks like one plate got most of the spores on down. I think I didn't realize how much or often to shake it up.

And I took rather small pieces for each jar of oats I think.

Thanks for your time


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Offlinebluerooms
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Re: Syringe not shaken enough? [Re: Inthepit]
    #26311094 - 11/10/19 08:30 AM (1 year, 5 months ago)

Shake the hell out of that thing.

If it's been in the fridge let it get to room temp and shake it, shake it, shake it.  :dancer:


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Invisiblepppp
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Re: Syringe not shaken enough? [Re: bluerooms]
    #26312817 - 11/11/19 02:48 AM (1 year, 5 months ago)

I used a Ms syringe for the first time not so long ago.
I was keeping it in the fridge.
I let only one drop fall into each agar plate and none germinated.

Second try was more successful.
As Blue mentioned let it warm up to room temperature, shake it really well, until you see spores floating around (they are hard to see tough)

Finally I added more then 1 drop that I spread around with an inoculation loop.
All except one plate germinated.

Good luck on your adventure :smile:


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OfflineInthepit
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Re: Syringe not shaken enough? [Re: pppp]
    #26315719 - 11/12/19 02:25 PM (1 year, 5 months ago)

Today I went back and added 3 or 4 more  .2" squares innoc to 3 jars. I'm thinking this is ok to help jump start. I think the innoc pieces were too small first try. I don't see any contamination.


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