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don_shadow

Registered: 11/06/19
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Myceliated Grain TEK 9
#26303098 - 11/06/19 02:24 PM (4 years, 3 months ago) |
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MYCELIATED GRAIN TEKIt has been found that the mycelium of certain species of potent psilocybin-bearing mushrooms of the genus Psilocybe also produces considerable quantities of active material when cultivated on sterilized, edible grain media (or “grain-spawn”). The grain-spawn is inoculated and incubated in glass canning jars or polyurethane bags specially designed to tolerate sterilization temperatures. The resulting myceliated grain is then dehydrated and easily preserved long-term if properly stored. The whole dried kernels may be simmered in water to create a potent tea, or ground to create an easily digestible flour (depending on the particular grain used). The two species known with certainty to produce active compounds in sufficient quantity are Psilocybe caerulescens, and Psilocybe hoogshagenii/semperviva, though it's likely that many others may be found to be equally effective once further research has been conducted. Ps. zapotecorum and Ps. mexicana are worthy candidates for experimentation. This method negates the need to fruit mushrooms, which can be costly, time-consuming and generally unfavorable to those without sufficient motivation or patience, particularly in the case of the lesser-known and temperamental psilocybin mushrooms such as Ps. caerulescens. The process is considerably simpler, less time consuming, and more discrete than fruiting mushrooms. It requires minimal equipment or expensive tools, and results in very high-quality material with little waste or environmental impact. Production requires little space, is easily scaled to suit the specific needs of the cultivator, and the quantity of inoculated material does not change the incubation time required for maximum potency. There is no need for a fruiting apparatus or incubator, and a flow-hood or glove-box is not required when a sterile liquid culture syringe is employed skillfully using filter lids fitted with self-healing injection ports. The creation of these lids requires a moderate level of patience and competency, but with tools readily obtained online or from a typical hardware store, dozens of durable, reusable lids may be created in a single afternoon. The lids are fastened on regular-mouthed or wide-mouthed glass canning jars, which serve as the final incubation vessels. Wide-mouthed jars are preferred for performing grain-to-grain inoculations. Once the grain has been inoculated, there is no need for further maintenance such as misting and fanning, so the grain jars or bags may be left unattended for extended periods of time without risk of failure. The substrate (hydrated grain) must be sterilized to ensure that competitor fungi and bacteria are eliminated before inoculating with the desired fungi. A pressure-cooker/canner capable of reaching temperatures necessary for rapid full sterilization will greatly ease the process and virtually eliminate the possibility of contamination when pure starting media is used, and when aseptic technique is followed correctly. However, sterilization by means of a pressure-canner is not required. Alternatively, a somewhat more tedious but sufficiently effective process called “fractional sterilization” or “Tyndallization” may be employed. This involves the staged immersion of jars in boiling water/steam in intervals over the course of three days. All that is required for this sterilization technique is a large pot with a well-fitting lid. Pure starting media is of critical importance for this method, as any contamination present in the master inoculant will be transmitted to the media that will ultimately be consumed. Fortunately, it is relatively easy to detect contamination on grain-spawn. Foul-smelling jars, or grain with visible signs of mold or bacteria should be discarded. Only grain with visibly healthy white mycelium and a typically “mushroomy” smell should be used, and only in the complete absence of doubt. The three most common starting media are spore-prints (mushroom spores stored in a tin-foil envelope), liquid culture (mycelium suspended in liquid nutrient media stored in a sterile syringe), and agar culture (mycelium grown on seaweed gelatin and stored in a petri dish, sterile test-tube or plastic baggie). Liquid culture syringes are preferred for this TEK as they can be expanded without the need for a sterile environment (such as a flow-hood or glove-box). Filter lids with self-healing injection-ports permit the application of liquid culture in open air. Spores on the other hand must be transferred and “cleaned” on sterile agar media, which can only be performed in a sterile environment. Likewise, agar cultures can only be expanded (to agar or liquid culture) in sterile conditions. In this tutorial, various modes of preparing nutrient media will be described in detail. It is warranted that at least a glove-box be created or obtained in order to permit the long-term storage and preservation of these rare and valuable species of fungi. Due to the legality and discrete nature of spore-prints, they will undoubtedly be the most common and sustainable mode of transferring the genetics through the mail (though a method for sending agar culture via standard lettermail will be described at the end of this manual). In order to sustain the demand for these rare fungi species, it will be necessary to fruit the mushrooms and obtain spore prints, therefore learning to cultivate the mushrooms themselves is strongly encouraged by the writers of this TEK. Ps. hoogshagenii/semperviva is quite simple to cultivate, and provides mushrooms of unparalleled quality. Some useful links with information regarding the cultivation of Ps. hoogshagenii/semperviva are included on the last page of the attached manual.  Enjoy!
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Asura
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Re: Myceliated Grain TEK [Re: don_shadow]
#26303163 - 11/06/19 02:55 PM (4 years, 3 months ago) |
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Good to see you, Don.
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R.I.P.Zappa
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Re: Myceliated Grain TEK [Re: Asura]
#26303422 - 11/06/19 05:23 PM (4 years, 3 months ago) |
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Interesting, was on my way to starting some stone jars and will give it a shot with the left over grain after removing the stones.
-------------------- -The heaviest thing one will ever carry is a thought- -"Like a Blind man In an orgy you gotta feel things out.".- -When we agree about our hallucinations, we call it “reality".- -If you defy authority because your told to, that's no better than blindly trusting authority.- psychonautwiki.org How it should & shouldn't look - NEW CULTIVATORS GUIDE BOD's Easy AF OAT prep tek. Principles of mushroom growing for beginners
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PrimalSoup
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Re: Myceliated Grain TEK [Re: don_shadow]
#26303954 - 11/06/19 11:22 PM (4 years, 3 months ago) |
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This is something I'll be trying.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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cronicr


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Re: Myceliated Grain TEK (moved) [Re: don_shadow] 2
#26303970 - 11/06/19 11:51 PM (4 years, 3 months ago) |
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This thread was moved from Mushroom Cultivation.
Reason: In cultivation we actually cultivate mushrooms.
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LogicaL Chaos
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Re: Myceliated Grain TEK [Re: don_shadow] 2
#26304049 - 11/07/19 01:48 AM (4 years, 3 months ago) |
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Ive done mycelium-only grows before. Its worth trying!
The trip is quite different. Very fast come up, with a very fast come down. Its like tea magnifed.
Ive found that consoldated PF cakes make the best mycelium extracts in my limited experience.
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Coffeeholic
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Thanks for this comprehensive TEK. I have downoladed it and will try this very soon
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Loaded Shaman
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Re: Myceliated Grain TEK [Re: don_shadow]
#26304078 - 11/07/19 02:23 AM (4 years, 3 months ago) |
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Interesting write-up.
Thank you!
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  "Real knowledge is to know the extent of one’s ignorance." — Confucius
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Asura
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Re: Myceliated Grain TEK [Re: PrimalSoup]
#26304685 - 11/07/19 10:29 AM (4 years, 3 months ago) |
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Quote:
PrimalSoup said: This is something I'll be trying. 
My understanding is that it's only been done with caerulescens and semperviva.
No one knows what species it works for and doesn't work for except those. Stone producers would probably be good candidates.
I have a huge bag of caerulescens that has been growing out for several weeks now. My plan is to test around Xmas
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don_shadow

Registered: 11/06/19
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Re: Myceliated Grain TEK [Re: Asura]
#26304847 - 11/07/19 11:30 AM (4 years, 3 months ago) |
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I recently heard a report from a trusted friend that the mycelium of Panaeolus cyanescens is not potent enough to be of any use in this application, so check that one off the list. Howdy Asura!
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Asura
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Re: Myceliated Grain TEK [Re: don_shadow]
#26304861 - 11/07/19 11:36 AM (4 years, 3 months ago) |
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Hey Don!
I do have a bag of pan cyan going. I think it was Mushinist that didn't have success with it. But I think he only let it go for a month.
I will say that when I broke up my bag for the first time there was some blueing so I am going to sample it at 2 months.
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don_shadow

Registered: 11/06/19
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Re: Myceliated Grain TEK [Re: Asura]
#26304981 - 11/07/19 12:18 PM (4 years, 3 months ago) |
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Cool, testing Panaeolus cyanescens mycelium at two months incubation time is worthy of experimentation for sure, thanks for doing the work.
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PrimalSoup
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Re: Myceliated Grain TEK (moved) [Re: cronicr]
#26305249 - 11/07/19 02:12 PM (4 years, 3 months ago) |
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Quote:
cronicr said: <img src='/forums/images/moved.gif'> This thread was moved from Mushroom Cultivation.
Reason: In cultivation we actually cultivate mushrooms.
Doesn't belong in TPE though, in TPE we only consume drugs or manufacture them from simple teks.
Put it in advanced mycology, it is that I think.
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if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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Asura
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Re: Myceliated Grain TEK (moved) [Re: PrimalSoup]
#26305524 - 11/07/19 04:06 PM (4 years, 3 months ago) |
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I don't think it belongs here either. It's going to get lost here. I think it has some pretty interesting implications that should be explored.
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KapnDank
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Re: Myceliated Grain TEK (moved) [Re: Asura]
#26305682 - 11/07/19 05:08 PM (4 years, 3 months ago) |
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Good to see you here Don and awesome Tek. I downloaded the PDF from mycotopia too. Any idea as to approx how much it takes for a dose equivalent to an average cube dose and how many would be in each quart jar? I could see large batches of tea being made into gummies utilizing this which would allow more people to experience a superior mushroom experience and makes storage easier and less space consuming. Also I agree this should be moved to where it won't get lost
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Edited by KapnDank (11/07/19 05:46 PM)
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KapnDank
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Re: Myceliated Grain TEK (moved) [Re: cronicr]
#26305752 - 11/07/19 05:42 PM (4 years, 3 months ago) |
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Quote:
cronicr said: <img src='/forums/images/moved.gif'> This thread was moved from Mushroom Cultivation.
Reason: In cultivation we actually cultivate mushrooms.
Ummm isn't all parts of the organism still considered part of the "mushroom"? seems like "cultivating fruitbodies" is the term you would be looking for to eliminate things you don't put into fruiting. I do believe growing stones still fall under the "mushroom cultivation" category even when they're only being grown for stones. Given that comparison I don't see why this Tek would be any different as they are both mycelium and can be harvested without fruiting, the stones are just dense masses of it. I know there's a bit of a stigma around here concerning consuming mycelia but that's been primarily around species that are relatively easy to fruit with lower actives found in the mycelium meaning you really gotta be lazy to even want to go that route. This is a different animal IMO. Lol it's discussing how to cultivate the actives of hard/timely to fruit species opening up the capability to use them as medicine where other species may not be as effective for them. I know JOC attests to different species being more useful for certain things and situations and I'm sure many other will too once it's within their reach. Anyways maybe I'm looking at it wrong but I feel like people's "feelings" played a larger part in this being moved outta mush cult than any logic behind it. However please enlighten me if I'm wrong. Lol I would actually prefer that I was. I have a lot of respect for this community and would hate to see something like this being somewhat swept under the rug for silly or not thought out reasons. Mush love everyone
Edited by KapnDank (11/07/19 05:44 PM)
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PrimalSoup
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Re: Myceliated Grain TEK (moved) [Re: KapnDank]
#26306071 - 11/07/19 08:31 PM (4 years, 3 months ago) |
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--------------------
if you stand too close to the machine it'll start to eat youPrimal's simple tested teks and projects: Wheat Prep 2.0 Acidic Tea Tek Potency Project!
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cronicr



Registered: 08/07/11
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Re: Myceliated Grain TEK (moved) [Re: PrimalSoup]
#26306087 - 11/07/19 08:41 PM (4 years, 3 months ago) |
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Yes but this is a method of getting a dose not a cultivation thing.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Asura
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Re: Myceliated Grain TEK (moved) [Re: cronicr]
#26306153 - 11/07/19 09:36 PM (4 years, 3 months ago) |
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I mean I totally get your point, but it's not quite a post that goes side by side with "post your DANK!!!" either 
Advanced Mycology on the other hand is dealing with cutting edge science involving "Extracting Psilocybin and Making Gummy Bears"
There are some interesting things to explore here. Like I've said before, it could be a gateway to building some more interest around harder to cultivate species. But maybe I am being naive in that regard, I admit.
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cronicr



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Re: Myceliated Grain TEK (moved) [Re: Asura]
#26306158 - 11/07/19 09:40 PM (4 years, 3 months ago) |
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I could have put it in advanced but there isn't much traffic there.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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