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OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 3 months
Re: Pasty Agar Tek [Re: Mr. Alien]
    #26302784 - 11/06/19 12:14 PM (4 years, 3 months ago)

Yea, that's part of my gripe with it. That and the fact that they mentioned it cannot be sterilized in a PC. I've had bad luck with everything requiring sterility so I'm going to err on the side of caution with this one and ban micropore tape from my grows.


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I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


Edited by McDominator (11/06/19 12:15 PM)


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InvisiblemushboyMDiscord
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Re: Pasty Agar Tek [Re: McDominator] * 1
    #26302791 - 11/06/19 12:18 PM (4 years, 3 months ago)

mp tape cant be sterilized? huh?
:what:

ive used mp tape on pastyplates for like 2yrs without any issues whatsoever.


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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Pasty Agar Tek [Re: mushboy]
    #26302794 - 11/06/19 12:20 PM (4 years, 3 months ago)

It's an ongoing debate which i believe centers around the company saying that it cannot to avoid law suits. We all know that it can and that it works well enough. But, i use SFD and will never go back to MP


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InvisiblemushboyMDiscord
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Re: Pasty Agar Tek [Re: verum subsequentis]
    #26302798 - 11/06/19 12:24 PM (4 years, 3 months ago)

yah but who puts sfds on minirounds. 2 layers of 3m tape worked wonderfully for me for awhile.


i use shitty postal tyvek squares held on with a layer or two of mp tape for lots of my lids and they all work just as well as poly(or unmodded for that matter)


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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
Posts: 8,732
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Re: Pasty Agar Tek [Re: mushboy]
    #26302806 - 11/06/19 12:26 PM (4 years, 3 months ago)

For sure. I don't use pasty plates anymore. They work fine but i like petris.


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OfflineMcDominator
Male


Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 3 months
Re: Pasty Agar Tek [Re: verum subsequentis]
    #26303073 - 11/06/19 02:17 PM (4 years, 3 months ago)

Well, perhaps I will experiment again with micropore tape just for the sake of experimenting. As verum said, 3m might just be trying to cover their ass to avoid law suites:



Quote:

3M said:

Question:

Is Micropore tape sterile? Can it be sterilized?

Answer:

3M Surgical tapes are sold clean, not sterile. Micropore tapes may be sterilized by ethylene oxide but NOT by steam (autoclave). Although 3M provides sterilization guidelines, it is up to the facility to ensure sterility. Sterrad™ processing with hydrogen peroxide is not recommended for Micropore tape because the rayon fibers in the tape will inactivate the hydrogen peroxide.




--------------------
I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism.


“It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.”

-Albert Einstein


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OfflineMerlin Gray
Merlin
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Registered: 11/06/19
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Loc: Georgia Flag
Last seen: 4 years, 3 months
Don't they melt in the PC? [Re: TheApprentice]
    #26303207 - 11/06/19 03:19 PM (4 years, 3 months ago)

I'm confused. Don't the Glad Mini's melt in the PC?
Thanks


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Offlineprimelines
Agarth Brooks
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Registered: 02/25/16
Posts: 484
Last seen: 30 days, 7 hours
Re: Don't they melt in the PC? [Re: Merlin Gray]
    #26303213 - 11/06/19 03:25 PM (4 years, 3 months ago)

Obviously not


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Invisible13shroomsM
Lightning Shaman
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Registered: 01/01/09
Posts: 26,719
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Re: Pasty Agar Tek [Re: verum subsequentis]
    #26303276 - 11/06/19 04:11 PM (4 years, 3 months ago)

Quote:

verum subsequentis said:
i use SFD and will never go back to MP




yup all because of this.. 



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:takingnotes:A M U:helpdesk:
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OfflineEdmunter
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Re: Pasty Agar Tek [Re: 13shrooms]
    #26303939 - 11/06/19 11:03 PM (4 years, 3 months ago)

Quote:

13shrooms said:
Quote:

verum subsequentis said:
i use SFD and will never go back to MP




yup all because of this.. 






I use MP on the sides of my rounds and I get very few contaminations


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InvisibleSpunkyMonkey88
Stranger
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Registered: 10/08/19
Posts: 1,331
Re: Pasty Agar Tek [Re: Edmunter]
    #26304107 - 11/07/19 03:22 AM (4 years, 3 months ago)

What is a SFD ?


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OfflineMag1cm4n
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Registered: 09/12/19
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Last seen: 1 year, 16 days
Re: Pasty Agar Tek [Re: SpunkyMonkey88]
    #26304218 - 11/07/19 05:47 AM (4 years, 3 months ago)

I'm not sure if it's just the brand of coloring I bought or what, but it seem that mycelium loves it and actually eats it right out of the agar? I may be way off but what are your thoughts?



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Offlineprimelines
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Registered: 02/25/16
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Re: Pasty Agar Tek [Re: Mag1cm4n]
    #26304225 - 11/07/19 05:52 AM (4 years, 3 months ago)

That's some nice growth, myc always eats the food colouring though.


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InvisibleSpunkyMonkey88
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Male


Registered: 10/08/19
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Re: Pasty Agar Tek [Re: primelines]
    #26304340 - 11/07/19 07:39 AM (4 years, 3 months ago)

So between my stove not being level and my tray inside of my PC not being level, my plates are off and the agar is slanted...

Should I pull them out while still hot and let em cool on a level surface or is it not a big deal? Also how deep should the agar be? I found this recipe only made enough for a very thin layer of aughgar so I doubled it, hope that's ok?

I'm assuming the medium shouldn't be translucent, correct?


Edited by SpunkyMonkey88 (11/07/19 08:57 AM)


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OfflineMag1cm4n
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Last seen: 1 year, 16 days
Re: Pasty Agar Tek [Re: primelines]
    #26305323 - 11/07/19 02:40 PM (4 years, 3 months ago)

Thanks! I could seriously watch myc grow all day haha. But that's good to know 😊


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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
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Re: Pasty Agar Tek [Re: SpunkyMonkey88]
    #26305347 - 11/07/19 02:48 PM (4 years, 3 months ago)

Quote:

SpunkyMonkey88 said:
What is a SFD ?



Synthetic Filter Disk. They are great.


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OfflineCapMeh
RX Jazzist
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Registered: 06/08/09
Posts: 924
Loc: The Holy Mountain
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Re: Pasty Agar Tek [Re: verum subsequentis]
    #26305491 - 11/07/19 03:50 PM (4 years, 3 months ago)

I don’t like MP tape.. If people use twist off lids they don’t have to modify the container at all.. it’s the way to go :super:


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OfflineMycoBrainz
Mycotic
Male User Gallery


Registered: 03/14/15
Posts: 616
Loc: West Coast
Last seen: 22 days, 15 hours
Re: Pasty Agar Tek [Re: verum subsequentis]
    #26320142 - 11/14/19 02:53 PM (4 years, 3 months ago)

Ok, I'm jealous. I just started with pyrex petri dish too. But it looks like I now have life goal to meet. Nice stack of petri dishes.


--------------------
:stoned:


PFC x Creeper


Lets Get Stoned


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OfflineMycoBrainz
Mycotic
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Registered: 03/14/15
Posts: 616
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Re: Pasty Agar Tek [Re: SpunkyMonkey88]
    #26320170 - 11/14/19 03:04 PM (4 years, 3 months ago)

Looks like your doing "slants" now, lol.


--------------------
:stoned:


PFC x Creeper


Lets Get Stoned


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Offlineprimelines
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Registered: 02/25/16
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Last seen: 30 days, 7 hours
Re: Pasty Agar Tek [Re: MycoBrainz]
    #26320261 - 11/14/19 03:33 PM (4 years, 3 months ago)

Quote:

MycoBrainz said:
Looks like your doing "slants" now, lol.



Can someone link me a good slant tek?


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