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primelines
Agarth Brooks


Registered: 02/25/16
Posts: 484
Last seen: 30 days, 7 hours
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Well you want enough water it wont all evaporate while pcing. You dont want too much water causing it to splash up over your plates. Elevate your plates off the bottom of the pc, I used a layer of jar lid rings on the bottom then the rack that came with the p . A little higher elevated is better so u can put in more water without the water going to high up the first layer of plates loaded into the pc.
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Edited by primelines (11/02/19 05:59 AM)
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Northern Thailand
Stranger

Registered: 08/29/19
Posts: 13
Last seen: 4 years, 2 months
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Primelines, thanks for your response.
Including using jar lids to raise up the rounds.
So... can you suggest how much water might be a minimum?
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,178
Loc: rural ghetto
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ive had great results by stabbing a bit from the cotton swab with your xacto knife and pulling away some bits of cotton, and then getting that to germinate in your agar plate. since i always make no pour ones, its better to use freshly made plates since it has more moisture than older plates, thus, making germination easier. with a single swab you can probably make 3-5 or more plates thats almost guaranteed to achieve some sort of germination, given your spores in the swabs are still viable.
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,178
Loc: rural ghetto
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edit: I didnt really read the question it seems, what I wrote was for PE and other hard-to-collect-spores swabs. xD
just leaving it there for others.
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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primelines
Agarth Brooks


Registered: 02/25/16
Posts: 484
Last seen: 30 days, 7 hours
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Depends what size pc you have. I never measure it out exactly. I should though. I just put the rings on the bottom, then the rack, load my plates and add water till it comes up to a bit under half way of the bottom layer of plates. If I'm not doing a shit load of plates, the plates float in the water so I put a dinner plate on top of the plates so they arent floating around. I put the plate as a weight on first then add my water so they dont float.
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Can someone please give me the ratios for this recipe, to make enough for 8 plastic minis? Is that what this recipe is for?
Sorry if that was clear in the tek, I was having a hard time understanding how much this was for...
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primelines
Agarth Brooks


Registered: 02/25/16
Posts: 484
Last seen: 30 days, 7 hours
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Yes it was for 8
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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most PCs have a fill line etched in the inside of the vessel. at least the prestos do. I always use that as a guide for water. hasnt failed me in 10years..
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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Northern Thailand
Stranger

Registered: 08/29/19
Posts: 13
Last seen: 4 years, 2 months
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thank you for the added clarity!!
Charlie
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Jow important is it to use micropore tape over the hole? I have this white cloth like tape that is similar but no exactly the same....
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,404
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Quote:
SpunkyMonkey88 said: Jow important is it to use micropore tape over the hole? I have this white cloth like tape that is similar but no exactly the same....
It does not have to be micropore tape. I've used polyfil for most of my pasty plates. Anything that can be bunched up tight enough to create a filter should work but polyfil and tape are so easy to get I can't imagine branching out for any reason.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,404
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Quote:
Mr. Alien said: I was thinking using PC sterilized cotton tips, a few dry and a few wet. Then, taking the dry one to rub the spore print to pick up some spore then use the wet tip to "steal" some spores from the dry one, then inoculate with the wet tip the agar plate. This should work i think?
Why are you thinking you need two different swabs? One dry swab is fine for grabbing the spores and Inoculating the plate.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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MagicJim
Stranger

Registered: 08/23/17
Posts: 23
Last seen: 3 days, 9 hours
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Forgot about this pasty white and this spirited lad blew his load
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McDominator



Registered: 08/29/19
Posts: 270
Loc: California
Last seen: 1 year, 3 months
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Micropore tape doesn't always work because, per 3m, it is not meant to filter small particles and cannot be sterilized using a pressure cooker. It's really not a great filter. All of my plates have failed using micropore tape. So the answer is that it probably depends on how clean your environment is whether or not you can get away with the tape.
I plan on using syringe filters in the future.
-------------------- I'm here to learn. I'm also willing to help. If I'm wrong on something, please call me out. I am not resistant to new information, but I always carry a healthy dose of skepticism. “It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a variation of wave pressure.” -Albert Einstein
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SpunkyMonkey88
Stranger



Registered: 10/08/19
Posts: 1,331
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Syringe filters sound overkill, I see many people successfully using tape on the lids with these, I'd think its gotta be getting in there some other way...
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JHOVA
Post whore


Registered: 02/17/17
Posts: 4,727
Loc:
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Tyvek + Mp is the shit
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the_sonic16
Mushy McMusherson



Registered: 03/10/19
Posts: 396
Loc: Somewhere off topic
Last seen: 2 years, 1 month
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Re: Pasty Agar Tek [Re: JHOVA]
#26300425 - 11/05/19 11:02 AM (4 years, 3 months ago) |
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Agreed. People had been using MP tape for years. Recently someone posted the package label. Now, MP hysteria has broken out where everyone is freaked out that it's going to contaminate their grows.
-------------------- Sonic's "I'll buy a pressure cooker someday" Instant Pot times and settings:
- Popping Corn Rapid Re-hydration - Half fill all containers with corn. Empty into Instant Pot. Cover with water. Run 30 minutes on PC. Release, remove, dry.
- Wheat/Rye Berry Soak Re-hydration - Half fill all containers with grain. Empty in pot with water. Soak for 6-12 hours, air dry until surface is completely dry.
- Grain Jar Sterilization - Run 2 - 2.5 hrs on PC
- PastyPlates, MEA Formulation- Run 45 minutes on PC
- LME Liquid Culture - Run 45 minutes on PC
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,178
Loc: rural ghetto
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5 layers of mp tape should extinguish the mp hysteria. xD
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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Mr. Alien
I will abduct andprobe your anus



Registered: 01/14/14
Posts: 6,290
Loc: Star Wars Galaxy
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MP is not even sterile from fabric.
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the_sonic16
Mushy McMusherson



Registered: 03/10/19
Posts: 396
Loc: Somewhere off topic
Last seen: 2 years, 1 month
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It seems to be working for this application though.
-------------------- Sonic's "I'll buy a pressure cooker someday" Instant Pot times and settings:
- Popping Corn Rapid Re-hydration - Half fill all containers with corn. Empty into Instant Pot. Cover with water. Run 30 minutes on PC. Release, remove, dry.
- Wheat/Rye Berry Soak Re-hydration - Half fill all containers with grain. Empty in pot with water. Soak for 6-12 hours, air dry until surface is completely dry.
- Grain Jar Sterilization - Run 2 - 2.5 hrs on PC
- PastyPlates, MEA Formulation- Run 45 minutes on PC
- LME Liquid Culture - Run 45 minutes on PC
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