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InvisibleTedsDead
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Registered: 01/03/17
Posts: 4,999
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: cronicr]
    #26284646 - 10/29/19 11:19 AM (4 years, 4 months ago)

:takingnotes:  thanks!


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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Offlineverum subsequentis
seeker of truth
I'm a teapot User Gallery


Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TedsDead]
    #26284654 - 10/29/19 11:22 AM (4 years, 4 months ago)

Quote:

TedsDead said:
hairy eyeball!?  did u tell her you're a stay at home bird therapist!?  you should go buy the same lot tomorrow if shes working again, lol

BTW, with those slants... Ive been filling mine with like 3.5 ml using a pipette its just enough to lay them completely flat and the cap is enough to slant the tube.  gives maximum surface area.

that verm on top? lol





Yeah, that's verm on top.

I like to have more agar in my slants because it causes them to last longer. Less surface area is kind of the whole point of slants in the first place. You only need the tiniest piece to get the culture going on fresh plates so i prefer more agar, more slant and less surface area.

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InvisibleTedsDead
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: verum subsequentis]
    #26284689 - 10/29/19 11:32 AM (4 years, 4 months ago)

:takingnotes:  ah.  your tubes look slightly wider than mine as well.  I like that.  I ordered some shiitake slants years ago and always just tried to replicate how the agar looked in that.  figured it was 'professional'.  I understand the theory tho so what your saying/doing makes sense

are you friends with the bird lady from mary poppins?

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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TedsDead]
    #26284693 - 10/29/19 11:34 AM (4 years, 4 months ago)

I am the bird lady from mary poppins.... and I'm willy myco. Who'da thunk

:mindblown:

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InvisibleTedsDead
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: verum subsequentis]
    #26284694 - 10/29/19 11:36 AM (4 years, 4 months ago)

your my hero, that lady is an OG boss!


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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Offlinevinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
Posts: 492
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TedsDead]
    #26285834 - 10/29/19 08:31 PM (4 years, 4 months ago)

I confess, I think I peed a little..... holyshit that was loud, the cats arent ever coming home


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OfflinecronicrFacebook
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: vinnie boombotz]
    #26285913 - 10/29/19 08:54 PM (4 years, 4 months ago)

U try to open it?


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Offlinevinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: cronicr]
    #26285931 - 10/29/19 09:01 PM (4 years, 4 months ago)

No, it just went pop

Last time I used it, I took out the Rubber seal and rubbed it down with coconut oil to keep it from drying out. I'm going to wipe down the lid and top rim and seal with alcohol, and try again. That might have been the problem. I'm kind of scared tho LOL it did scare the s*** out of me

Edited by vinnie boombotz (10/29/19 09:07 PM)

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Offlinespiritlands
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: vinnie boombotz]
    #26285960 - 10/29/19 09:17 PM (4 years, 4 months ago)

Quote:

vinnie boombotz said:
I confess, I think I peed a little..... holyshit that was loud, the cats arent ever coming home





Ugh... I confess I did something similar once. Have a bad habit of doing this stuff while the fam sleeps and I have some beers. Guess I got tired and fell asleep waiting for the timer on the stove... PC went dry and was on high until the morning when I found I melted the seal just like in yours. I think this is why they recommend lubing the seal bc after a while it starts to adhere and doesn't expand to fill the seal evenly. I'm lucky I didn't start a fire.


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:



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InvisibleTedsDead
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: spiritlands]
    #26285964 - 10/29/19 09:19 PM (4 years, 4 months ago)

soooo lucky.  did u see the pic of that exploded stove like a week ago some one posted... fuuuck:facepalm3:


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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Offlinespiritlands
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TedsDead]
    #26285975 - 10/29/19 09:31 PM (4 years, 4 months ago)

No I didn't see The blown up stove but I want to... The one I melted had a pressure gauge bc it was for sterilizing tattoo equipment not food. The pressure release gauge failed that night too thankful it still vented.


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:



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Offlinevinnie boombotz
Reggaejunkiejew


Registered: 04/29/19
Posts: 492
Last seen: 9 months, 4 days
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: spiritlands]
    #26285985 - 10/29/19 09:41 PM (4 years, 4 months ago)




so far so good, but I ain't trying to go near it

Edit: success!! Lesson learned, greasy seals are no no

Edited by vinnie boombotz (10/29/19 10:42 PM)

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OfflinePuduwoke
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Registered: 06/25/17
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: vinnie boombotz]
    #26286110 - 10/29/19 11:44 PM (4 years, 4 months ago)

I confess .. 3 days ago I did about 10 transfers and left 2 plates open in front of my new flowhood. All but 3 jars are covered in contams.

Im hoping it could be a few things

I attached a net in front the hood to protect it. The net was not new but I took it from a building site I'm currently working at. I could imagine it's far from clean.

Also I was running the blower on pretty much the lowest speed to keep noise down.
Only ran the hood for about 15 min before starting work as well. (Its located in a tiny room)

Will run hood for longer before starting as well as taking of the net and increasing the speed of the blower.

I think one or more if these things is the problem.

Really really hope so as well!
:prayer:


--------------------
:sporedrop:Trade List:sporedrop:

LAGM2021

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InvisibleTedsDead
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Puduwoke]
    #26286170 - 10/30/19 12:42 AM (4 years, 4 months ago)

whoa, there... net? job site?  pic???  its probably not your open plates.  did you test for laminar flow at the lowest speed?


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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OfflinePuduwoke
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TedsDead]
    #26286295 - 10/30/19 02:57 AM (4 years, 4 months ago)

How do I test for laminar flow?
No picture of the net. Just a normal "chicken net".


--------------------
:sporedrop:Trade List:sporedrop:

LAGM2021

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OfflinemeaculpaUIO
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Registered: 08/26/19
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Puduwoke]
    #26286342 - 10/30/19 03:58 AM (4 years, 4 months ago)

I confess I have used up my first 100 bax of one time use gloves and that makes me feel good

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Offlinespiritlands
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: meaculpaUIO]
    #26286792 - 10/30/19 09:58 AM (4 years, 3 months ago)

I spray the intake and exhaust on my flow hood when I turn it on and before I start any work. The net is prob your problem for sure, can't blow clean air past a dirty net and not blow contams around. Maybe use a section of chicken wire instead of net? It's cheap and not porous but will hold your filter in place.


--------------------


Germinating old spores progress page
how to calibrate your Martha



:spiralflip::spiralflip:



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OfflinePuduwoke
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Registered: 06/25/17
Posts: 1,666
Last seen: 15 hours, 39 minutes
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: spiritlands]
    #26286859 - 10/30/19 10:45 AM (4 years, 3 months ago)

I took the net off and are currently staring at 2 open plates as they are sitting in front of the flow hood.

Will tell you guys in a few days how it went!

Thanks for the feedback.


--------------------
:sporedrop:Trade List:sporedrop:

LAGM2021

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InvisibleTedsDead
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Re: TRUE CONFESSIONS - of an amateur mycologist [Re: Puduwoke]
    #26286871 - 10/30/19 10:48 AM (4 years, 3 months ago)

Quote:

Puduwoke said:
How do I test for laminar flow?
No picture of the net. Just a normal "chicken net".




a bic lighter at 12" away from the filter should bend 90 degrees but not go out.


--------------------
weed gets you through times of no money better than money gets you through times of no weed...  -the fabulous furry freak bros
If you can buy it, you can burn it!



https://www.shroomery.org/forums/showflat.php/Number/25947396#25947396

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Invisibleforagedfungus
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Registered: 09/30/13
Posts: 1,851
Loc: out there
Re: TRUE CONFESSIONS - of an amateur mycologist [Re: TedsDead] * 1
    #26286900 - 10/30/19 11:01 AM (4 years, 3 months ago)

The "lighter test" will give an approximation of airspeed.
You can use smoke to visually see if the flow is laminar.

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