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Offlinecoldworldsoldier
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Spore prints question
    #26282001 - 10/28/19 12:29 PM (1 year, 4 months ago)

Maybe a dumb question....If one were to take a print from every harvest wouldn't be possible to keep growing and never need to buy spores again? I vaguely remember reading somewhere about losing potency over time. If I grew a batch and took a print...then grew from that print and took a print from that harvest.


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OfflineIstrati92
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Re: Spore prints question [Re: coldworldsoldier]
    #26282010 - 10/28/19 12:33 PM (1 year, 4 months ago)

You you do things right  you can grow  all ir life from a print, and you can print all ur shrooms every  harvest


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Offlinecoldworldsoldier
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Re: Spore prints question [Re: Istrati92]
    #26282024 - 10/28/19 12:36 PM (1 year, 4 months ago)

That's what I assumed...it only seems logical. Maybe I was reading that you can only isolate agar so many times? I know I read somewhere about genetics getting weak eventually. I believe RR mentioned something about this somewhere..not quite sure


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Offlinesbkn
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Re: Spore prints question [Re: coldworldsoldier]
    #26282028 - 10/28/19 12:38 PM (1 year, 4 months ago)

yes you could always have spores to use, however, learning to use agar (which is incredibly easy) provides you with an unlimited source for growing mushrooms, but ALSO, a much safer guarantee that the mycelium will not be contaminated and healthier/faster mycelium growth. As you've probably heard, and despite any success you've had with spores, using them is a gamble each time which can be eliminated by using agar.


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Offlinecoldworldsoldier
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Re: Spore prints question [Re: sbkn]
    #26282060 - 10/28/19 12:49 PM (1 year, 4 months ago)

I've done agar...actually just inoculated some today. I had old pasty plate that grew a mush that sporulated in the container. It dates like that for over a year. I'm hoping I can get some healthy growth out of it considering it's been so long. All my syringes are that old also...I'd rather not have to order more.


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Re: Spore prints question [Re: coldworldsoldier]
    #26282095 - 10/28/19 01:01 PM (1 year, 4 months ago)

Your line will never get weaker if you just use spores every time.  The only time a strain gets weaker, in theory, if it is isolated or expanded again and again, I believe.


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Offlinecoldworldsoldier
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Re: Spore prints question [Re: naturalistic123]
    #26282144 - 10/28/19 01:23 PM (1 year, 4 months ago)

Yes...I think it was g2g transfers that I read about. Now I just need to pray that the spores I have are still viable


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OfflinePsilo_citizen
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Re: Spore prints question [Re: coldworldsoldier]
    #26282371 - 10/28/19 02:58 PM (1 year, 4 months ago)

Over many generations, heterozygosity will decrease. Most organisms don't fare well from prolonged periods of inbreeding.

That being said, most won't ever run into issues. I've read a bit suggesting that it can result in a reduction of available mating types resulting in longer perceived germination periods(longer to see dikaryotic mycelium which is what we look for, not really germination, but I would say it falls into the germination period category for practical hobby cultivation purposes) though I may be a bit off base on this; don't quote me.


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Re: Spore prints question [Re: coldworldsoldier]
    #26282442 - 10/28/19 03:28 PM (1 year, 4 months ago)

Quote:

coldworldsoldier said:
Maybe a dumb question....If one were to take a print from every harvest wouldn't be possible to keep growing and never need to buy spores again? I vaguely remember reading somewhere about losing potency over time. If I grew a batch and took a print...then grew from that print and took a print from that harvest.



This is  called selfing and not great in the long run but spores can last year's cultures even longer.
Take prints early, slant cultures stick it to the man


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Offlineiux
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Re: Spore prints question [Re: cronicr]
    #26282509 - 10/28/19 03:57 PM (1 year, 4 months ago)

You could easily do grows your entire life from just a tiny percentage of one print, once you get your first grow, maybe you get 40 mushrooms, you can print all shrooms twice, maybe thrice (Prints will be a bit lighter, but more than enough spores will deposit on just a tiny spec of one print), so there, from one to 120 prints, repeat this three times, and you can have almost two million prints! Nature is awesome :smile:

It is insanely exponential, one print is all you need, but, it's handy to have more in case you want to try something else.

Be sure to keep the earliest prints you have, even after using them several times for print to agar transfer, you never know if you might need it again, besides, they last for decades, if not a lot, lot longer if stored properly.

I bet some prints could probably last more than 50 years, the main problem would be rehydrating them, but I wager it would be possible! :biggrin:


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Offlineiux
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Re: Spore prints question [Re: cronicr]
    #26282516 - 10/28/19 04:02 PM (1 year, 4 months ago)

Quote:

cronicr said:
Quote:

coldworldsoldier said:
Maybe a dumb question....If one were to take a print from every harvest wouldn't be possible to keep growing and never need to buy spores again? I vaguely remember reading somewhere about losing potency over time. If I grew a batch and took a print...then grew from that print and took a print from that harvest.



This is  called selfing and not great in the long run but spores can last year's cultures even longer.
Take prints early, slant cultures stick it to the man





A question, so, let's say if one starts with three prints and you want to keep going for a long time, on just three prints, would a good way to keep the genetics healthy over time be to mix two jars of different kind and then fruit them in the same monotub?

Would this make mycelium tangle together and make hybrids of sorts? Or would the best way perhaps be to start at the spore stage, so mixing spores from two different prints (I suspect this would work alright since this is how it mostly happens in nature?)


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The mycological world is amazing! Be respectful to eachothers :hug: :eskimokiss:

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Re: Spore prints question [Re: iux]
    #26283120 - 10/28/19 08:29 PM (1 year, 4 months ago)

It may or may not, mix the spores and it will.


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Re: Spore prints question [Re: cronicr]
    #26283199 - 10/28/19 09:01 PM (1 year, 4 months ago)

Let’s put it this way. I had a print from 2001 and 2003 and they germinated in 5 days. I grew out a few flushes of each. A print can last a long time. One print can easily give you enough to continually grow forever. All you need to store is 1 print. I usually keep 3 of each for personal just in case. But spores can last a long time. And I’ve kept the spore prints stored in a dorm fridge that long.


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Offlinecoldworldsoldier
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Re: Spore prints question [Re: eatyualive]
    #26290245 - 10/31/19 06:54 PM (1 year, 3 months ago)

Somewhat related to another comment above: Would it be beneficial to take a spore print from 2 separate shrooms? Print one and then the other directly over top. Both of these being 2 different strains therefore enabling them to be mixed and not be "inbred" or "selfing" as stated previously?


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OfflinePsilocyBen17
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Re: Spore prints question [Re: naturalistic123]
    #26290424 - 10/31/19 08:23 PM (1 year, 3 months ago)

This is true but I've been running the same strain for about a year (maybe 30 transfers on agar?) and I havent had problems.


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OfflinePsilocyBen17
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Re: Spore prints question [Re: PsilocyBen17]
    #26290426 - 10/31/19 08:24 PM (1 year, 3 months ago)

come to think of it I havent really heard of anyone on here having trouble from it.


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Offlinespiritlands
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Re: Spore prints question [Re: PsilocyBen17]
    #26290572 - 10/31/19 09:26 PM (1 year, 3 months ago)

The genetic diversity of the spores of one fruit are prob equivalent to the spores from another fruit even though they might have different substrains that produced them -if they are from the same grow.

That is unless a new phenotype shows up (like red spore or albino) those spores should have a higher percentage of spores carrying genes for that phenotype/mutation.

It would be interesting to know if mushroom traits are recessive or dominant or incomplete dominant. This would change the distribution of genes among the spores.


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