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OfflineTonyMushrana
is that a Trich again?
I'm a teapot User Gallery


Registered: 03/12/19
Posts: 323
Loc: Mountainly Place
Last seen: 1 year, 10 months
First A2G transfer, is my plate looking OK to be used?
    #26277393 - 10/26/19 02:18 AM (4 years, 3 months ago)

Hei, so I am planning to do the transfer todoay, have some jars ready.

Here are pics of petri, whats your opinion guys?






Sincerly, Tony


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: First A2G transfer, is my plate looking OK to be used? [Re: TonyMushrana]
    #26277406 - 10/26/19 02:41 AM (4 years, 3 months ago)

Cool. I like to cut a circle around the myc, then quarter it.


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OfflineTonyMushrana
is that a Trich again?
I'm a teapot User Gallery


Registered: 03/12/19
Posts: 323
Loc: Mountainly Place
Last seen: 1 year, 10 months
Re: First A2G transfer, is my plate looking OK to be used? [Re: Feasoghorm]
    #26277408 - 10/26/19 02:50 AM (4 years, 3 months ago)

Hei GypsyCurse, thanks for reply.

I have 6 1QT jars
You mean like to cut circle of living mycelium and leave uncolonised part?
Is it enough for 6 jars?
Should I leave a litte bit of mycelium edge?


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: First A2G transfer, is my plate looking OK to be used? [Re: TonyMushrana]
    #26277573 - 10/26/19 06:46 AM (4 years, 3 months ago)

I do two pucks per jar. So if i was gonna a2g 6 jars i'd need 12 clean plates. I quarter the circle of myc so there is more inoculation points in which to colonize the jar. A2g takes for god damn ever to colonize. I use to cut all kinda tick tac toe patters in the pucks to make a shitload of wedges and jst dump them in, but that process ended up being a contam magnet for me.


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OfflineCoffeeholic
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Registered: 09/18/19
Posts: 74
Last seen: 5 months, 9 days
Re: First A2G transfer, is my plate looking OK to be used? [Re: Feasoghorm]
    #26277617 - 10/26/19 07:23 AM (4 years, 3 months ago)

Quote:

GypsyCurse said:
Cool. I like to cut a circle around the myc, then quarter it.



Like a donut, and don't use the middle?


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InvisibleFeasoghorm

Registered: 10/24/18
Posts: 4,384
Re: First A2G transfer, is my plate looking OK to be used? [Re: Coffeeholic]
    #26277683 - 10/26/19 07:59 AM (4 years, 3 months ago)

What? No, the middle is the part you use. That's the bit with the myc on it.

The idea behind not letting the myc grow to the edge of the plate, is that the edge of the plate is the most likely place for contams to appear. You dnt wanna transfer contams.

So i basically cut around the silver dollar sized circle of myc, then make one horizontal and one virtical cut thru the myc making four wedges of agar.


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