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Offlinesoad05
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Registered: 10/24/03
Posts: 6
Last seen: 11 years, 5 months
use part of a syringe?
    #2626925 - 05/01/04 07:12 PM (12 years, 7 months ago)

Hey, im just a newbie and my 20 mL EQ syringe just arrvied. However, im going on a trip (vacation :smile: ) in about 7 weeks so im not gonna risk sarting a whole new grow with 12-20 cakes and miss out on the fruiting. However, i would like to maybe grow 1 or 2 in orer to test out and sewe whter the groing conditions are substantial here and so that i can perfect the art of sterility before i uses the whole syringe.Its not really a problem if i miss the a ferw flushes , i just want to achieve pinning by doing a dry run. What im wondering is if i can use just part of the spore syringe, then re-cap the needle (or sterilze or whatever) and then store it (where should i store them anyway) untill im ready to start the bulkish grow. Thanks for the help.


--------------------
"I didn't go to college, and I'm kickass"
~Jack Black


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 191,595
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Re: use part of a syringe? [Re: soad05]
    #2626928 - 05/01/04 07:14 PM (12 years, 7 months ago)

place it in 2 ziplocks and pop it in the fridge... it'll keep at room temperature but under refridgeration it lasts longer


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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
Re: use part of a syringe? [Re: soad05]
    #2626932 - 05/01/04 07:15 PM (12 years, 7 months ago)

just recap it, stick it in a ziplock baggie and put it in the back of your fridge...

when you're ready to use it again, flame the needle to sterilise it (cool by squirting a tiny amount of solution out) and away you go :wink:


--------------------
Shrooms aren't everyone's cup of tea... (Some folks just eat 'em)


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Offlinesoad05
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Registered: 10/24/03
Posts: 6
Last seen: 11 years, 5 months
Re: use part of a syringe? [Re: sakura]
    #2626952 - 05/01/04 07:22 PM (12 years, 7 months ago)

thanks for the help, the syringes are 10 not 20mL. How many black dot should i be able to see in an average syringe anways?


--------------------
"I didn't go to college, and I'm kickass"
~Jack Black


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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
Re: use part of a syringe? [Re: soad05]
    #2626997 - 05/01/04 07:54 PM (12 years, 7 months ago)

it matters very little...  even if your syringes appear totally clear, there is still a LOT of spores in there :wink: (those things are microscopic man)


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Shrooms aren't everyone's cup of tea... (Some folks just eat 'em)


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Offlinegermin8tionn8ion
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Re: use part of a syringe? [Re: soad05]
    #2627011 - 05/01/04 08:09 PM (12 years, 7 months ago)

Quote:

soad05 said:
Hey, im just a newbie and my 20 mL EQ syringe just arrvied. However, im going on a trip (vacation :smile: ) in about 7 weeks so im not gonna risk sarting a whole new grow with 12-20 cakes and miss out on the fruiting. However, i would like to maybe grow 1 or 2 in orer to test out and sewe whter the groing conditions are substantial here and so that i can perfect the art of sterility before i uses the whole syringe.Its not really a problem if i miss the a ferw flushes , i just want to achieve pinning by doing a dry run. What im wondering is if i can use just part of the spore syringe, then re-cap the needle (or sterilze or whatever) and then store it (where should i store them anyway) until im ready to start the bulkish grow. Thanks for the help.




My recommendation would be to boil some water and mix it with Karo syrup, one table spoon of syrup for about 100ml of water. Knock up your jars that you want to do, then suck up that honey water to fill the syring, and incubate it.  Lets say you have a 20mL syringe and you use 15 mL of it for 15 jars. Then you do what I suggested and incubate for a few weeks. you'll have 20 mL of liquid mycelia for inoculations


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Offlineberto23
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Registered: 04/26/04
Posts: 250
Loc: NJ
Last seen: 3 years, 8 months
Re: use part of a syringe? [Re: germin8tionn8ion]
    #2627466 - 05/02/04 11:27 AM (12 years, 7 months ago)

wont the mycelia clump together and not be able tog et out of the syringe??


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the shnozzberrys taste like shnozzberrys


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InvisibleMystiqueMushroom


Registered: 11/01/11
Posts: 4,627
Loc: PNW
Re: use part of a syringe? [Re: berto23]
    #15358254 - 11/12/11 01:01 AM (5 years, 25 days ago)

if you dont want to make a lc(liquid culture), then you can take cap off put on a sterile piece of tin foil, fold sides, put back on when done innoculating


edit: old thread oops :pipesmoke:


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:sunny:


Edited by MystiqueMushroom (11/12/11 01:15 AM)


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