Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Mono Tub Substrate   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
A strain crossing thread * 18
    #26254384 - 10/15/19 04:25 PM (4 years, 4 months ago)

Who wants to have some fungi fun?
Let's cross some varieties and make it happen! We have been making some awesome crosses over the years and you don't need to be a pro to do it, if you can grow a of cake it's possible.
Crossing is very simple or very complicated depending on the route you wish to take, it can be as easy as mixing spores and growing them out or as hard as making monokaryons and hoping they are compatible.
Because cubes are cubes spores are a very simple method to go with, swipe prints from albino a + and some ksss and put it to some plates and your success rate is 25% minimum. The trick here is spotting the cross which one would hope is as simple as grow g it and looking for a white little saucer in the mix but it isn't the case, more often then not you will simply see completely normal cubensis on your first generation with more expression coming in later gens so don't sweat it.
Here's  a great example

I'll leave it at this for now and will edit this post with serial dilution methods and once I have permission will show u the crosses that are being made right now like the new albino penis envies  and leucistic penis envies Holden halo and ksss ECT.


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
InvisibleSubnet Mask
 User Gallery

Registered: 05/08/15
Posts: 903
Loc: Flag
Re: A strain crossing thread [Re: cronicr]
    #26254392 - 10/15/19 04:32 PM (4 years, 4 months ago)

:popcorn:


Extras: Filter Print Post Top
InvisibleSigSaur99
Human

Registered: 10/08/19
Posts: 164
Loc: Moscow, Russia
Re: A strain crossing thread [Re: cronicr] * 2
    #26254393 - 10/15/19 04:33 PM (4 years, 4 months ago)

This is great, Imagine Crossing PE6 with B+ =)


Extras: Filter Print Post Top
OfflineJiles
Shwomp
 User Gallery


Registered: 08/13/13
Posts: 98
Last seen: 1 year, 3 months
Re: A strain crossing thread [Re: cronicr] * 1
    #26254399 - 10/15/19 04:35 PM (4 years, 4 months ago)

How does the cross actually breed genetics between the next generation of spores?

I always thought that by having separate species on the same substrate although they would grow would still be considered two separate strains and have separate mycelium community's even tho its all bonded togather? So like some fruits may look like one variety vs some may look like the other or how does this work?


--------------------
You can do what you want and pick what you like, but you cant always get what you want!


Extras: Filter Print Post Top
InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh 😛
I'm a teapot User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 6,067
Trusted Cultivator
Re: A strain crossing thread [Re: Jiles] * 1
    #26254417 - 10/15/19 04:49 PM (4 years, 4 months ago)

:popcorn: Count me in Cron!


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: A strain crossing thread [Re: Jiles]
    #26254418 - 10/15/19 04:50 PM (4 years, 4 months ago)

Quote:

Jiles said:
How does the cross actually breed genetics between the next generation of spores?

I always thought that by having separate species on the same substrate although they would grow would still be considered two separate strains and have separate mycelium community's even tho its all bonded togather? So like some fruits may look like one variety vs some may look like the other or how does this work?



Fruits are made of dikaryotic mycelium
Dikaryons are the result of two monokaryons fusing, each monokaryon has half a set of genetics that make a fruit.
When a spore germinate it is a monokaryon, so two spores germinate to become a dikaryon.
So if one spore was from Aa+ the other from pe you will have a leucistic pe


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh 😛
I'm a teapot User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 6,067
Trusted Cultivator
Re: A strain crossing thread [Re: cronicr]
    #26254423 - 10/15/19 04:53 PM (4 years, 4 months ago)

Out of curiosity....can this be done with 2 different variety grain spawn or does it have to happen at spore level? :takingnotes:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: A strain crossing thread [Re: Tormato] * 5
    #26254426 - 10/15/19 04:55 PM (4 years, 4 months ago)

Dikaryons can fuse it's got a much lower success rate, if you put two isolates on a plate 9 times out of ten they just form a wall of aversion that even Trump would be proud of


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
InvisibleTormatoMFacebookDiscordReddit
The Goddess Kali Meh 😛
I'm a teapot User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 6,067
Trusted Cultivator
Re: A strain crossing thread [Re: cronicr]
    #26254443 - 10/15/19 05:01 PM (4 years, 4 months ago)

Sweet! I've been looking for a challenge....this might be right up my alley :cheers:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Extras: Filter Print Post Top
OfflineJiles
Shwomp
 User Gallery


Registered: 08/13/13
Posts: 98
Last seen: 1 year, 3 months
Re: A strain crossing thread [Re: cronicr]
    #26254444 - 10/15/19 05:02 PM (4 years, 4 months ago)

Quote:

cronicr said:
Quote:

Jiles said:
How does the cross actually breed genetics between the next generation of spores?

I always thought that by having separate species on the same substrate although they would grow would still be considered two separate strains and have separate mycelium community's even tho its all bonded togather? So like some fruits may look like one variety vs some may look like the other or how does this work?



Fruits are made of dikaryotic mycelium
Dikaryons are the result of two monokaryons fusing, each monokaryon has half a set of genetics that make a fruit.
When a spore germinate it is a monokaryon, so two spores germinate to become a dikaryon.
So if one spore was from Aa+ the other from pe you will have a leucistic pe




Ok this makes clear sense now thank you! So would it be recommended when beginning to try something like this by maybe making liquid cultures so that the spores may germinate together as intended versus trying to merge colonies of grain?


--------------------
You can do what you want and pick what you like, but you cant always get what you want!


Extras: Filter Print Post Top
InvisibleSir Pentinite
Stranger all the time.


Registered: 05/15/19
Posts: 525
Loc: ation Location Location
Re: A strain crossing thread [Re: Jiles]
    #26254459 - 10/15/19 05:09 PM (4 years, 4 months ago)

:threadmonitor:


--------------------
"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: A strain crossing thread [Re: Jiles] * 1
    #26254461 - 10/15/19 05:10 PM (4 years, 4 months ago)

Quote:

Jiles said:
Quote:

cronicr said:
Quote:

Jiles said:
How does the cross actually breed genetics between the next generation of spores?

I always thought that by having separate species on the same substrate although they would grow would still be considered two separate strains and have separate mycelium community's even tho its all bonded togather? So like some fruits may look like one variety vs some may look like the other or how does this work?



Fruits are made of dikaryotic mycelium
Dikaryons are the result of two monokaryons fusing, each monokaryon has half a set of genetics that make a fruit.
When a spore germinate it is a monokaryon, so two spores germinate to become a dikaryon.
So if one spore was from Aa+ the other from pe you will have a leucistic pe




Ok this makes clear sense now thank you! So would it be recommended when beginning to try something like this by maybe making liquid cultures so that the spores may germinate together as intended versus trying to merge colonies of grain?



Agar first


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineSvetaketu
The Devil's Avocado 🥑
Male


Registered: 10/08/15
Posts: 1,508
Loc: United States
Last seen: 18 hours, 31 minutes
Re: A strain crossing thread [Re: cronicr] * 1
    #26254631 - 10/15/19 06:28 PM (4 years, 4 months ago)

I'm already all in for trying to isolate monokaryons :rofl:

I get that there are simpler ways to cross (swab technique you mentioned and many others) but I'm kinda just into wasting a bunch of personal time trying to isolate a monokaryon... Call me masochistic, but the science behind it is fascinating.

Anyone else read that old Post where someone mentions isolating single spores with a microscope and a 30g needle? Well... I've been trying it, and it's definitely tedious and difficult... But not as hard as it sounds actually.

I'm still waiting to see some germination on any of the plates (5 days in) but it really appeared like I got some single spores on the needle, and onto a petri.

Is there a better way to go about this? Next on my list is serial dilution if this doesn't work out... Workman's streak method sounded pretty "simple" too, but seems to waste a lot of plates.


--------------------
LAGM2020
LAGM2021


Extras: Filter Print Post Top
OfflineCthulhu23
Elder God Extraordinaire
Male


Registered: 07/04/18
Posts: 620
Last seen: 2 years, 7 months
Re: A strain crossing thread [Re: Svetaketu]
    #26254651 - 10/15/19 06:34 PM (4 years, 4 months ago)

:threadmonitor:


Extras: Filter Print Post Top
OfflineElViajero
Traveler


Registered: 08/16/18
Posts: 295
Last seen: 2 years, 10 months
Re: A strain crossing thread [Re: Cthulhu23]
    #26254670 - 10/15/19 06:41 PM (4 years, 4 months ago)

:takingnotes: i love where this thread is going... has anyone tried to mix Redspore with a leucistic variety?


--------------------


Extras: Filter Print Post Top
InvisiblemushboyMDiscord
modboy
 User Gallery


Registered: 04/24/05
Posts: 32,394
Loc: where?
Trusted Cultivator
OG Cultivator
Re: A strain crossing thread [Re: ElViajero] * 2
    #26254676 - 10/15/19 06:44 PM (4 years, 4 months ago)

:camping:


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: A strain crossing thread [Re: ElViajero]
    #26254685 - 10/15/19 06:51 PM (4 years, 4 months ago)

Quote:

ElViajero said:
:takingnotes: i love where this thread is going... has anyone tried to mix Redspore with a leucistic variety?



Yep I am on my second gen right now


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 26 days
Trusted Cultivator
Re: A strain crossing thread [Re: Svetaketu]
    #26254689 - 10/15/19 06:52 PM (4 years, 4 months ago)

Serial dilution is fairly simple and if you use no pours you don't waisted plates just resterilize and use them again


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineSvetaketu
The Devil's Avocado 🥑
Male


Registered: 10/08/15
Posts: 1,508
Loc: United States
Last seen: 18 hours, 31 minutes
Re: A strain crossing thread [Re: cronicr]
    #26254814 - 10/15/19 08:04 PM (4 years, 4 months ago)

I do have some no-pour plates I was using earlier in the year :strokebeard:

Looking forward to reading some serial dilution methods :thumbup:


--------------------
LAGM2020
LAGM2021


Extras: Filter Print Post Top
OfflineCthulhu23
Elder God Extraordinaire
Male


Registered: 07/04/18
Posts: 620
Last seen: 2 years, 7 months
Re: A strain crossing thread [Re: ElViajero] * 1
    #26254818 - 10/15/19 08:08 PM (4 years, 4 months ago)

Quote:

ElViajero said:
:takingnotes: i love where this thread is going... has anyone tried to mix Redspore with a leucistic variety?




Isnt that what RW is?  CRS x AA+?


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | Next > | Last >

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Mono Tub Substrate   PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* cross linking genes Anonymous 1,607 12 10/15/02 02:50 AM
by shroomsformike
* P Strain Not pinning strain 3,271 6 03/25/02 08:23 AM
by GaNjAShRooM
* What to do with these P strain cakes/casings? strain 2,322 1 04/20/02 04:08 PM
by World Spirit
* Melmac TP questions BigwormS 2,046 10 11/24/22 02:34 AM
by Roasted
* STRAINS
( 1 2 3 all )
doc34 6,537 57 06/18/04 11:07 PM
by Magash
* what happens when you knock up jars with two diferent strain uneasyone 903 6 09/07/03 08:18 AM
by OJK
* cross germination of strains mattch1 1,701 13 11/18/03 01:20 AM
by The_LosT_SouL
* 2 strains in one casing peruvian spark 1,027 8 02/07/03 10:10 AM
by peruvian spark

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
26,657 topic views. 35 members, 208 guests and 30 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.006 seconds on 13 queries.