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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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A strain crossing thread 18
#26254384 - 10/15/19 04:25 PM (4 years, 4 months ago) |
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Who wants to have some fungi fun? Let's cross some varieties and make it happen! We have been making some awesome crosses over the years and you don't need to be a pro to do it, if you can grow a of cake it's possible. Crossing is very simple or very complicated depending on the route you wish to take, it can be as easy as mixing spores and growing them out or as hard as making monokaryons and hoping they are compatible. Because cubes are cubes spores are a very simple method to go with, swipe prints from albino a + and some ksss and put it to some plates and your success rate is 25% minimum. The trick here is spotting the cross which one would hope is as simple as grow g it and looking for a white little saucer in the mix but it isn't the case, more often then not you will simply see completely normal cubensis on your first generation with more expression coming in later gens so don't sweat it. Here's a great example  I'll leave it at this for now and will edit this post with serial dilution methods and once I have permission will show u the crosses that are being made right now like the new albino penis envies and leucistic penis envies Holden halo and ksss ECT.
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Subnet Mask


Registered: 05/08/15
Posts: 903
Loc:
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Re: A strain crossing thread [Re: cronicr]
#26254392 - 10/15/19 04:32 PM (4 years, 4 months ago) |
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SigSaur99
Human

Registered: 10/08/19
Posts: 164
Loc: Moscow, Russia
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Re: A strain crossing thread [Re: cronicr] 2
#26254393 - 10/15/19 04:33 PM (4 years, 4 months ago) |
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This is great, Imagine Crossing PE6 with B+ =)
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Jiles
Shwomp



Registered: 08/13/13
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Re: A strain crossing thread [Re: cronicr] 1
#26254399 - 10/15/19 04:35 PM (4 years, 4 months ago) |
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How does the cross actually breed genetics between the next generation of spores?
I always thought that by having separate species on the same substrate although they would grow would still be considered two separate strains and have separate mycelium community's even tho its all bonded togather? So like some fruits may look like one variety vs some may look like the other or how does this work?
-------------------- You can do what you want and pick what you like, but you cant always get what you want!

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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: A strain crossing thread [Re: Jiles] 1
#26254417 - 10/15/19 04:49 PM (4 years, 4 months ago) |
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Count me in Cron!
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: A strain crossing thread [Re: Jiles]
#26254418 - 10/15/19 04:50 PM (4 years, 4 months ago) |
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Quote:
Jiles said: How does the cross actually breed genetics between the next generation of spores?
I always thought that by having separate species on the same substrate although they would grow would still be considered two separate strains and have separate mycelium community's even tho its all bonded togather? So like some fruits may look like one variety vs some may look like the other or how does this work?
Fruits are made of dikaryotic mycelium Dikaryons are the result of two monokaryons fusing, each monokaryon has half a set of genetics that make a fruit. When a spore germinate it is a monokaryon, so two spores germinate to become a dikaryon. So if one spore was from Aa+ the other from pe you will have a leucistic pe
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: A strain crossing thread [Re: cronicr]
#26254423 - 10/15/19 04:53 PM (4 years, 4 months ago) |
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Out of curiosity....can this be done with 2 different variety grain spawn or does it have to happen at spore level?
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: A strain crossing thread [Re: Tormato] 5
#26254426 - 10/15/19 04:55 PM (4 years, 4 months ago) |
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Dikaryons can fuse it's got a much lower success rate, if you put two isolates on a plate 9 times out of ten they just form a wall of aversion that even Trump would be proud of
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Tormato  
The Goddess Kali Meh 😛




Registered: 07/01/17
Posts: 6,067
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Re: A strain crossing thread [Re: cronicr]
#26254443 - 10/15/19 05:01 PM (4 years, 4 months ago) |
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Sweet! I've been looking for a challenge....this might be right up my alley
-------------------- Helpful Threads The Shroomery Store Tormato's Q&A Thread Post Questions Here or PM me! "Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!
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Jiles
Shwomp



Registered: 08/13/13
Posts: 98
Last seen: 1 year, 3 months
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Re: A strain crossing thread [Re: cronicr]
#26254444 - 10/15/19 05:02 PM (4 years, 4 months ago) |
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Quote:
cronicr said:
Quote:
Jiles said: How does the cross actually breed genetics between the next generation of spores?
I always thought that by having separate species on the same substrate although they would grow would still be considered two separate strains and have separate mycelium community's even tho its all bonded togather? So like some fruits may look like one variety vs some may look like the other or how does this work?
Fruits are made of dikaryotic mycelium Dikaryons are the result of two monokaryons fusing, each monokaryon has half a set of genetics that make a fruit. When a spore germinate it is a monokaryon, so two spores germinate to become a dikaryon. So if one spore was from Aa+ the other from pe you will have a leucistic pe
Ok this makes clear sense now thank you! So would it be recommended when beginning to try something like this by maybe making liquid cultures so that the spores may germinate together as intended versus trying to merge colonies of grain?
-------------------- You can do what you want and pick what you like, but you cant always get what you want!

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Sir Pentinite
Stranger all the time.


Registered: 05/15/19
Posts: 525
Loc: ation Location Location
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Re: A strain crossing thread [Re: Jiles]
#26254459 - 10/15/19 05:09 PM (4 years, 4 months ago) |
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-------------------- "I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'" - Terrence McKenna
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: A strain crossing thread [Re: Jiles] 1
#26254461 - 10/15/19 05:10 PM (4 years, 4 months ago) |
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Quote:
Jiles said:
Quote:
cronicr said:
Quote:
Jiles said: How does the cross actually breed genetics between the next generation of spores?
I always thought that by having separate species on the same substrate although they would grow would still be considered two separate strains and have separate mycelium community's even tho its all bonded togather? So like some fruits may look like one variety vs some may look like the other or how does this work?
Fruits are made of dikaryotic mycelium Dikaryons are the result of two monokaryons fusing, each monokaryon has half a set of genetics that make a fruit. When a spore germinate it is a monokaryon, so two spores germinate to become a dikaryon. So if one spore was from Aa+ the other from pe you will have a leucistic pe
Ok this makes clear sense now thank you! So would it be recommended when beginning to try something like this by maybe making liquid cultures so that the spores may germinate together as intended versus trying to merge colonies of grain?
Agar first
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Svetaketu
The Devil's Avocado 🥑



Registered: 10/08/15
Posts: 1,508
Loc: United States
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Re: A strain crossing thread [Re: cronicr] 1
#26254631 - 10/15/19 06:28 PM (4 years, 4 months ago) |
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I'm already all in for trying to isolate monokaryons 
I get that there are simpler ways to cross (swab technique you mentioned and many others) but I'm kinda just into wasting a bunch of personal time trying to isolate a monokaryon... Call me masochistic, but the science behind it is fascinating.
Anyone else read that old Post where someone mentions isolating single spores with a microscope and a 30g needle? Well... I've been trying it, and it's definitely tedious and difficult... But not as hard as it sounds actually.
I'm still waiting to see some germination on any of the plates (5 days in) but it really appeared like I got some single spores on the needle, and onto a petri.
Is there a better way to go about this? Next on my list is serial dilution if this doesn't work out... Workman's streak method sounded pretty "simple" too, but seems to waste a lot of plates.
-------------------- LAGM2020 LAGM2021
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Cthulhu23
Elder God Extraordinaire



Registered: 07/04/18
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Re: A strain crossing thread [Re: Svetaketu]
#26254651 - 10/15/19 06:34 PM (4 years, 4 months ago) |
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ElViajero
Traveler


Registered: 08/16/18
Posts: 295
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Re: A strain crossing thread [Re: Cthulhu23]
#26254670 - 10/15/19 06:41 PM (4 years, 4 months ago) |
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i love where this thread is going... has anyone tried to mix Redspore with a leucistic variety?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,394
Loc: where?
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Re: A strain crossing thread [Re: ElViajero] 2
#26254676 - 10/15/19 06:44 PM (4 years, 4 months ago) |
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: A strain crossing thread [Re: ElViajero]
#26254685 - 10/15/19 06:51 PM (4 years, 4 months ago) |
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Quote:
ElViajero said:
i love where this thread is going... has anyone tried to mix Redspore with a leucistic variety?
Yep I am on my second gen right now
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 26 days
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Re: A strain crossing thread [Re: Svetaketu]
#26254689 - 10/15/19 06:52 PM (4 years, 4 months ago) |
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Serial dilution is fairly simple and if you use no pours you don't waisted plates just resterilize and use them again
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Svetaketu
The Devil's Avocado 🥑



Registered: 10/08/15
Posts: 1,508
Loc: United States
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Re: A strain crossing thread [Re: cronicr]
#26254814 - 10/15/19 08:04 PM (4 years, 4 months ago) |
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I do have some no-pour plates I was using earlier in the year
Looking forward to reading some serial dilution methods
-------------------- LAGM2020 LAGM2021
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Cthulhu23
Elder God Extraordinaire



Registered: 07/04/18
Posts: 620
Last seen: 2 years, 7 months
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Re: A strain crossing thread [Re: ElViajero] 1
#26254818 - 10/15/19 08:08 PM (4 years, 4 months ago) |
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Quote:
ElViajero said:
i love where this thread is going... has anyone tried to mix Redspore with a leucistic variety?
Isnt that what RW is? CRS x AA+?
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