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Spirit-Crusher
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Anyone have luck doing jar to bags in a SAB?
#26245535 - 10/11/19 04:52 PM (4 years, 3 months ago) |
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Trying to get this technique down. Anyone have any hints or tips?
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k5hd2y
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Re: Anyone have luck doing jar to bags in a SAB? [Re: Spirit-Crusher] 1
#26245553 - 10/11/19 05:01 PM (4 years, 3 months ago) |
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Tip use small jars and a big tub. 108 felt too small to me. I would make a big sab or flowhod or inject lc into presealed bags.
Maybe someone else can be more helpful. It was so uncomfortable i abandoned it for most things.
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dschill
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Re: Anyone have luck doing jar to bags in a SAB? [Re: k5hd2y]
#26245929 - 10/11/19 07:28 PM (4 years, 3 months ago) |
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What type of jars? Like grain jars?
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JHOVA
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Re: Anyone have luck doing jar to bags in a SAB? [Re: dschill] 1
#26245987 - 10/11/19 07:56 PM (4 years, 3 months ago) |
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ask stareatclouds. he does it with a vertical sab.
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cronicr



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Re: Anyone have luck doing jar to bags in a SAB? [Re: JHOVA] 1
#26246088 - 10/11/19 08:27 PM (4 years, 3 months ago) |
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I flip my sab upside down so my wormholes are higher toward the top
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san pedro guy
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Re: Anyone have luck doing jar to bags in a SAB? [Re: cronicr]
#26246192 - 10/11/19 09:16 PM (4 years, 3 months ago) |
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I wish my sab had wormholes, would make it so much easier...
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cronicr



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Re: Anyone have luck doing jar to bags in a SAB? [Re: san pedro guy] 1
#26246216 - 10/11/19 09:28 PM (4 years, 3 months ago) |
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fucking auto Arm holes*
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Spirit-Crusher
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Re: Anyone have luck doing jar to bags in a SAB? [Re: dschill]
#26247308 - 10/12/19 12:22 PM (4 years, 3 months ago) |
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Yeah.
Im doing 1 quart jars of grain spawn to a 5lb bag of rye. Just trying to stream line these ambitious projects I've been doing. Im just tired of working 100's of jars. I have near 100% success with jars in a SAB though. So far I've wasted countless hours and money trying to dial in bags.
I just want to know if people do this regularly without a flow hood. If the success rate is a low percentage I'll just stop wasting my time and spawn until I get a flow hood.
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K3yBoardC0WBoy
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Re: Anyone have luck doing jar to bags in a SAB? [Re: Spirit-Crusher] 1
#26247608 - 10/12/19 02:40 PM (4 years, 3 months ago) |
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Sterilize my bulk sub in bags for pans and just empty the colonized jar into the bag, shake to distribute, and seal.
Works fine for me so far...
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Ifkilbadluck
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Re: Anyone have luck doing jar to bags in a SAB? [Re: K3yBoardC0WBoy]
#26247653 - 10/12/19 03:01 PM (4 years, 3 months ago) |
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I have great success doing it. Hell when I started I only had a 55qt sab and did 5lb bags. I still only use a sab. My success rates have been too high for me to want to build a flow hood yet. When I had a small sab it was tricky though. I had to keep the bags somewhat rolled up with just a little bit of the top of the bag sticking up (6in or so). It is a challenge when you do not have enough height in the sab. I still used the small sab once in a while out of laziness and I do bag to bag transfers at this point so I wipe my bags with iso roll put in sab and then take my spawn bag reseal it really low to the grain and cut the excess off. I mainly do these runs in my small sab to save my isolate/clone just Incase it flushes really really good I can keep it going and save the genetics but I have very few issues. In my experience as long as nothing touches the inside of the bag you will be fine.
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stareatclouds
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Re: Anyone have luck doing jar to bags in a SAB? [Re: JHOVA] 1
#26247660 - 10/12/19 03:07 PM (4 years, 3 months ago) |
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Quote:
JHOVA said: ask stareatclouds. he does it with a vertical sab.

Do this. Everybody looks for the tallest and widest SAB they can find, but nobody just flips the fucker on the end. Saran is clear, tight, and blocks drafts. The lid to the tote should be your opening where you wrap with Saran and cut holes in. There's more I could say, but want to finalize an optimal MVP before I start recommending it.
Note: This was my original SAB so the armholes are already cut in. They're not in use for this design and should be ignored as they're taped over.
I have a few modifications I'm trying out and don't want to say too much before the Tek, but I'm having solid success with this. My last 4 bags all colonized and fruited fine. 2 hit 3 flushes, another is on the 3rd now, and I'm about to harvest the 2nd on the 4th bag. This would mark what I believe is the 24th successful bag I've inoculated in my SAB and fruited. My biggest bottleneck so far is learning how to prep and sterilize bags.
I've also done several CVG and spawn bags with liquids successfully. Most can probably manage with a syringe in a smaller tote, but I dumped liquid in a few and got more than a few attempts to fruit multiple flushes.
You don't need a plastic tote. Just something you can see through that will stop drafts and be fine to work in. I haven't had time to try this, but I see no reason why you can't just use the racking on literally any wire shelf and wrap it in Saran. Go get some PVC pipe and construct the frame of a wide and tall rectangle and wrap that bitch in Saran. I'm sure it'll work.
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ShroomyToons
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Re: Anyone have luck doing jar to bags in a SAB? [Re: stareatclouds]
#26247780 - 10/12/19 03:47 PM (4 years, 3 months ago) |
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I've done it before I got my flow hood.
It works, even in a 66 quart SAB, but you just have to go slow. Doing 2 bags at once was doable, but not more than that. If you need more, reset the SAB and do it again.
Patience is critical. And be all set up so you don't pull arms in and out.
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Spirit-Crusher
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Re: Anyone have luck doing jar to bags in a SAB? [Re: stareatclouds]
#26683921 - 05/20/20 06:00 PM (3 years, 8 months ago) |
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Quote:
stareatclouds said:
Quote:
JHOVA said: ask stareatclouds. he does it with a vertical sab.

Do this. Everybody looks for the tallest and widest SAB they can find, but nobody just flips the fucker on the end. Saran is clear, tight, and blocks drafts. The lid to the tote should be your opening where you wrap with Saran and cut holes in. There's more I could say, but want to finalize an optimal MVP before I start recommending it.
Note: This was my original SAB so the armholes are already cut in. They're not in use for this design and should be ignored as they're taped over.
I have a few modifications I'm trying out and don't want to say too much before the Tek, but I'm having solid success with this. My last 4 bags all colonized and fruited fine. 2 hit 3 flushes, another is on the 3rd now, and I'm about to harvest the 2nd on the 4th bag. This would mark what I believe is the 24th successful bag I've inoculated in my SAB and fruited. My biggest bottleneck so far is learning how to prep and sterilize bags.
I've also done several CVG and spawn bags with liquids successfully. Most can probably manage with a syringe in a smaller tote, but I dumped liquid in a few and got more than a few attempts to fruit multiple flushes.
You don't need a plastic tote. Just something you can see through that will stop drafts and be fine to work in. I haven't had time to try this, but I see no reason why you can't just use the racking on literally any wire shelf and wrap it in Saran. Go get some PVC pipe and construct the frame of a wide and tall rectangle and wrap that bitch in Saran. I'm sure it'll work.
Well I'm gonna finally try this again. I ran out of jars and honestly I don't feel like buying another 100. Im also tired of washing those god damn things all the time,plus storing them. I guess my only question is the tyvek sleeve absolutely necessary? Im gonna make sure the grains are dryer than normal, and pressure cook them for 3 hours at 15 psi. Any other tips?
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mushpunx
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Re: Anyone have luck doing jar to bags in a SAB? [Re: Spirit-Crusher] 1
#26683994 - 05/20/20 06:32 PM (3 years, 8 months ago) |
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The tyvek sleeve isn't absolutely necessary. You need good form though, there is always dead skin cells falling off our arms and it will have bacteria a ND stuff so you never move them over top open sterile media in an SAB. Good idea to wash and dry your arms first.
Jar to bag isn't so hard. Usually I will put a full quart jar into a 4-5qt spawn bag. You just angle the spawn bag towards the jar, pull open the mouth of the bag and hold it with one hand while pouring in your grain with your other hand. Then close the mouth of the bag, fold + twist. In an SAB you probably want to have a zip tie or tie wire ready, unless you have a really big SAB I think it would be a pain to use an impulse sealer.
You don't need to stand the bag up. I am working with a 12 x 24" hood right now, it's shorter than most SABs. You just angle the spawn bag.
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Stromrider
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Re: Anyone have luck doing jar to bags in a SAB? [Re: mushpunx]
#26684054 - 05/20/20 06:58 PM (3 years, 8 months ago) |
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How are you guys filling the bag with air without pulling in dirty air?
If youre taking a whole quart of spawn to 1 spawn bag you could probably do it in open air and get away with it. It's going to colonize in a week
A flowhood isn't a huge step dudes. If you want to do bags go for it. You'll never regret it. Then you can step it up and start doing bag to bag. Living on edge my friends. Do it every week
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Spirit-Crusher
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Re: Anyone have luck doing jar to bags in a SAB? [Re: mushpunx]
#26684091 - 05/20/20 07:16 PM (3 years, 8 months ago) |
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Quote:
mushpunx said: The tyvek sleeve isn't absolutely necessary. You need good form though, there is always dead skin cells falling off our arms and it will have bacteria a ND stuff so you never move them over top open sterile media in an SAB. Good idea to wash and dry your arms first.
Jar to bag isn't so hard. Usually I will put a full quart jar into a 4-5qt spawn bag. You just angle the spawn bag towards the jar, pull open the mouth of the bag and hold it with one hand while pouring in your grain with your other hand. Then close the mouth of the bag, fold + twist. In an SAB you probably want to have a zip tie or tie wire ready, unless you have a really big SAB I think it would be a pain to use an impulse sealer.
You don't need to stand the bag up. I am working with a 12 x 24" hood right now, it's shorter than most SABs. You just angle the spawn bag.
I wear tyvek sleeves and constantly wipe them down with alcohol. I feel more confident going in this time, but still nervous. I just have visions of losing all my spawn because I decided to go this route instead of just spawning my jars.
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jcm4620
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Re: Anyone have luck doing jar to bags in a SAB? [Re: Spirit-Crusher]
#26684100 - 05/20/20 07:20 PM (3 years, 8 months ago) |
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i do it with pans all the time also and i dont even use a sab for spawning to my bags
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mushpunx
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Re: Anyone have luck doing jar to bags in a SAB? [Re: Stromrider]
#26684485 - 05/20/20 10:57 PM (3 years, 8 months ago) |
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Quote:
Stromrider said: How are you guys filling the bag with air without pulling in dirty air?
If youre taking a whole quart of spawn to 1 spawn bag you could probably do it in open air and get away with it. It's going to colonize in a week
A flowhood isn't a huge step dudes. If you want to do bags go for it. You'll never regret it. Then you can step it up and start doing bag to bag. Living on edge my friends. Do it every week
When I used to work with spawn bags in a still air box, after it was inoculated and sealed I would massage the bag to mix it up. I believe a small amount of air would be pulled into the bag through the filter patch.
That is the downside to working with bags in a still air box though, you can't inflate a plenum of sterile air into the bag like you can with a flow hood.
I would never G2G in open air though, no matter how much spawn I was pouring into the receiver. In a still air box, sure.
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mushpunx
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Re: Anyone have luck doing jar to bags in a SAB? [Re: jcm4620]
#26684490 - 05/20/20 11:00 PM (3 years, 8 months ago) |
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Quote:
jcm4620 said: i do it with pans all the time also and i dont even use a sab for spawning to my bags
What is in the bags you are spawing to? You can spawn into pasteurized substrate in open air all you like. I would never grain to grain transfer or spawn to a sterile substrate in open air though. Too much of a risk.
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mushpunx
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Re: Anyone have luck doing jar to bags in a SAB? [Re: Spirit-Crusher]
#26684504 - 05/20/20 11:05 PM (3 years, 8 months ago) |
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Quote:
Spirit-Crusher said:
Quote:
mushpunx said: The tyvek sleeve isn't absolutely necessary. You need good form though, there is always dead skin cells falling off our arms and it will have bacteria a ND stuff so you never move them over top open sterile media in an SAB. Good idea to wash and dry your arms first.
Jar to bag isn't so hard. Usually I will put a full quart jar into a 4-5qt spawn bag. You just angle the spawn bag towards the jar, pull open the mouth of the bag and hold it with one hand while pouring in your grain with your other hand. Then close the mouth of the bag, fold + twist. In an SAB you probably want to have a zip tie or tie wire ready, unless you have a really big SAB I think it would be a pain to use an impulse sealer.
You don't need to stand the bag up. I am working with a 12 x 24" hood right now, it's shorter than most SABs. You just angle the spawn bag.
I wear tyvek sleeves and constantly wipe them down with alcohol. I feel more confident going in this time, but still nervous. I just have visions of losing all my spawn because I decided to go this route instead of just spawning my jars.
Personally, I think bags are a pain in SAB, I would rather work with jars but it definitely can be done.
Just make sure you have big enough arm holes on your SAB so the tyvek sleeves don't stir up the air.
Instead of using alcohol you can sterilize your sleeves rolled up in spawn jars or bags with a 20 min PC cycle. I like to keep a few pairs of sleeves, I just leave them sterile rolled up in the jar until it's time to work. It's certainly not necessary but it's easy enough.
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