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Invisiblegeorge castanzaM
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E. coli bioengineered to produce psilocybin * 1
    #26220221 - 09/30/19 11:24 AM (4 years, 3 months ago)

https://www.sciencealert.com/scientists-made-a-bacterium-that-poops-out-psilocybin


It appears that they are producing over a gram of psilocybin per liter.


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OfflineDorfnob
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Re: E. coli bioengineered to produce psilocybin [Re: george castanza]
    #26220356 - 09/30/19 12:31 PM (4 years, 3 months ago)

guess we need to step up our game. and hire ninjas:ninjalike: to jack us some bacteria cultures


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OfflineJizzMasterZero
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Re: E. coli bioengineered to produce psilocybin [Re: Dorfnob]
    #26220747 - 09/30/19 03:42 PM (4 years, 3 months ago)

Naw, people will just bitch about it being a GMO.


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OfflineSizlChest
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Re: E. coli bioengineered to produce psilocybin [Re: JizzMasterZero]
    #26220891 - 09/30/19 04:44 PM (4 years, 3 months ago)

Fucking incredible. We have no idea what we are capable of...


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"Hell, shrooms have blown up from under me and kicked my ass on 2 grams once."
"I think ill eat some shrooms right about now, and ill continue to until it doesnt feel like the right thing to do."


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OfflineDorfnob
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Re: E. coli bioengineered to produce psilocybin [Re: SizlChest]
    #26220954 - 09/30/19 05:13 PM (4 years, 3 months ago)

lol for real people are ridiculous about gmo's and its really nothing new to re-engineer e.coli its just a surprise decide to make psilocybin.


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OfflineShroomei
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Re: E. coli bioengineered to produce psilocybin [Re: Dorfnob]
    #26221468 - 09/30/19 11:01 PM (4 years, 3 months ago)

There is a work group in Germany that hopes to sell it to pharmaceutical industry. If you can produce it cheap that will boost the interest in medical research of psilocybin use.


Edited by Shroomei (10/01/19 04:00 PM)


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Invisiblenooneman
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Re: E. coli bioengineered to produce psilocybin [Re: george castanza]
    #26221477 - 09/30/19 11:14 PM (4 years, 3 months ago)

I'd rather have the LSD producing e coli. We've already got lots of good fungi that produce psilocybin.

I mean, it's an impressive achievement and all, but kinda pointless considering how easy mushrooms are to grow. Unless someone was planning on needing unbelievably large quantities on a regular basis which seems somewhat doubtful.


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InvisibleMostly_HarmlessM
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Re: E. coli bioengineered to produce psilocybin [Re: nooneman]
    #26221659 - 10/01/19 03:53 AM (4 years, 3 months ago)

posted here, paper attached


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OfflineShroomei
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Re: E. coli bioengineered to produce psilocybin [Re: nooneman] * 1
    #26222875 - 10/01/19 03:40 PM (4 years, 3 months ago)

Quote:

nooneman said:
I'd rather have the LSD producing e coli. We've already got lots of good fungi that produce psilocybin.

I mean, it's an impressive achievement and all, but kinda pointless considering how easy mushrooms are to grow. Unless someone was planning on needing unbelievably large quantities on a regular basis which seems somewhat doubtful.



If you want to develop it as medication, do clinical testing and sell it later you want to have huge quantities as cheap as possible.
E.coli is way more easier to cultivate than funghi and already accepted as a host for production.
The LSD producing E.coli is worth a thought. Let's do some research and start the PCR machines.


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Invisiblenooneman
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Re: E. coli bioengineered to produce psilocybin [Re: Shroomei]
    #26222896 - 10/01/19 03:55 PM (4 years, 3 months ago)

That's a good point, that makes way more sense now :thumbup:

Quote:

Shroomei said:
The LSD producing E.coli is worth a thought. Let's do some research and start the PCR machines.



:drooling:

Can you imagine? If you could get a vat of e coli to produce even one gram, that's 10,000 hits, 100 sheets.


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OfflineShroomei
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Re: E. coli bioengineered to produce psilocybin [Re: nooneman]
    #26222925 - 10/01/19 04:19 PM (4 years, 3 months ago)

Quote:



Can you imagine? If you could get a vat of e coli to produce even one gram, that's 10,000 hits, 100 sheets.




Couldn't find an n-ethyl-transferase with google and stitching together the pathway for lysergic acid might be tough....

Btw: The E.coli were fed with hydroxy-indole as precursor for psilocybin. Without it the culture won't produce much psilocybin if any.
:aweshit:


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Invisiblegeorge castanzaM
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Re: E. coli bioengineered to produce psilocybin [Re: Shroomei]
    #26252123 - 10/14/19 03:23 PM (4 years, 3 months ago)

Quote:

Shroomei said:
Quote:



Can you imagine? If you could get a vat of e coli to produce even one gram, that's 10,000 hits, 100 sheets.




Couldn't find an n-ethyl-transferase with google and stitching together the pathway for lysergic acid might be tough....

Btw: The E.coli were fed with hydroxy-indole as precursor for psilocybin. Without it the culture won't produce much psilocybin if any.
:aweshit:




So to fully understand the meaning of this: the e.coli was fed a synthetic substance that was a precursor to the expected production of psilocybin, and the actual value of the e.coli production of psilocybin is directly linked to such precursor chemical, that itself is not naturally occurring and/or is not extraordinarily cheap, and in all actually this method of producing the desired chemical is not very innovative, or much of a "breakthrough".

Or, if I am totally off base, please expand on your comment to the degree you are able to comfortably explain, as I am mostly ignorant of basic chemistry and synthetic production of certain chemicals,  but I am willing to learn.


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OfflineShroomei
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Re: E. coli bioengineered to produce psilocybin [Re: george castanza]
    #26262405 - 10/19/19 05:29 AM (4 years, 3 months ago)

Quote:

george castanza said:

Or, if I am totally off base, please expand on your comment to the degree you are able to comfortably explain, as I am mostly ignorant of basic chemistry and synthetic production of certain chemicals,  but I am willing to learn.




Imagine chemical synthesis as building with Lego. You have a basic block -the precursor- and sequentially add or taking away further blocks as you need. Chemistry uses reactive chemicals -biology uses enzymes and less reactive chemicals.

I just did dome speed reading of the article so I might did not get all..
The article said that e.coli can't put the hydroxyl group on because it lacked the enzyme. So their bug won't make (any or much) psilocybin on its own. To be efficient it needs to be fed the precursor hydroxy-indole which is an industrial chemical to convert it to psilocybin. Hydroxy-indole brings the hydroxyl group with it.
However they could have put all enzymes in the bug to mimick what the shroom does. Then the e.coli won't need hydroxy-indole it would use its own tryptophane.

Why they did not chose this way I don't know. Maybe it did not work this way or it was just more efficient the other way.
Or it gives you some control. Growing e.coli is a piece of cake and having one producing tons of a schedule I drug roaming around would be disconcerting. While selling industry chemicals is tightly controlled. So the bug alone won't do any good to a clandestine lab without a supply of hydroxy-indole.

It still is cool to let an organism do what it can do better than chemistry and vice versa. So getting the best of two worlds. Very much as with LSD. Synthesizing lysergic acid is difficult the shroom can do it better giving the precursor for the last chemical synthesis step to LSD.

Hope that helps.


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OfflineShroomei
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Re: E. coli bioengineered to produce psilocybin [Re: george castanza]
    #26262407 - 10/19/19 05:34 AM (4 years, 3 months ago)

Quote:

george castanza said:[/b

So to fully understand the meaning of this: the e.coli was fed a synthetic substance that was a precursor to the expected production of psilocybin, and the actual value of the e.coli production of psilocybin is directly linked to such precursor chemical, that itself is not naturally occurring.




Yes. However, basic industry chemicals can be suprisingly cheap.

Sorry, forgot to answer that in the previous post.
.


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