Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1 | 2 | 3  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Big or small agar wedges to jars
    #26201238 -

So im getting close to transferring my agar plates to grain and im just wondering what would be better a whole bunch of small pieces of agar or 3 or 4 bigger pieces im gonna have enough plates to put one plate per jar so i could make lots of agar wedges for the jars and speed up colonization also should i shake the jars immediately after putting agar wedges in or just leave them on top of grain until they start colonizing then shake the jars

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26201247 -

I was doing that for a while, now I just scrape the surface of the agar with an inoculation loop.


--------------------

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: SawCeeJack]
    #26201250 -

and make LI ?

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26201251 -

approximately 1cm x 1cm put 3 pieces in whatever your colonizing and shake :brainscream:


--------------------



Edited by Tornarsuk (09/21/19 01:44 PM)

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26201265 -

insutama said:
and make LI ?


First off, I use no-pour half-pint jars that have been PC'd.  I then scrape the surface off the agar with the inoculation loop (after sterilizing with an alcohol lamp) into the cracked lid of the grain jar.  I do this partly so I don't have to drag the piece of agar through the air.  I just slide it into the jar like dumping spaghetti into a trash can.


--------------------

Edited by SawCeeJack (09/21/19 01:50 PM)

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: SawCeeJack]
    #26201288 -

i have 1 plate per jar so i would be using alot more than 3 pieces but ill do 1cm by 1cm pieces

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: SawCeeJack]
    #26201291 -

AtmozFear said:
insutama said:
and make LI ?


First off, I use no-pour half-pint jars that have been PC'd.  I then scrape the surface off the agar with the inoculation loop (after sterilizing with an alcohol lamp) into the cracked lid of the grain jar.  I do this partly so I don't have to drag the piece of agar through the air.  I just slide it into the jar like dumping spaghetti into a trash can.


So you pour the mycelium liquid into the jars and leave the agar in the no pour ?

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26201299 -

well that's when I'm making the agar.  but what i was talking about comes after a colony has been established after about five days.

you should just shoot over to a no-pour tek to understand that part better...


--------------------

Edited by SawCeeJack (09/21/19 02:16 PM)

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: SawCeeJack]
    #26201337 -

All up to personal preference.  You can do the tic tac toe method, especially if you're using no-pour plates.  Mentioned here:
https://www.shroomery.org/forums/showflat.php/Number/22601023

You can do the same thing with pour agar plates, but it's more tedious. 

Personally, I just cut a very large chunk of agar or two or three (depending on how many clean plates I have ready at the moment) and dump them in. 

Small pieces = more inoculation points
Larger pieces = easier to get one section of the jar to colonize quickly and a little bit less tedious and less chance to introduce contamination into your dish/jar due to more time spent cutting on the dish.  However, if you're sterile technique is on point, you don't really have to worry too much about the higher risk of contams being introduced.

But, assuming your plate is clean, grains are clean, and technique is sound you'll be fine whichever way you choose:goodluck:


--------------------
Loneliness is a term used by those that aren't comfortable with themselves
Once one conquers loneliness, others are never needed to find happiness

Edited by in_dot (09/21/19 02:40 PM)

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: in_dot]
    #26201348 -

I wouldnt put an entire plate to a jar. The point of using wedges is to leave behind latent/invisible contamination. I use a .5x.5x.5 triangle and either throw the culture in cold storage or in the trash.


--------------------
REPENT

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: jbgtaa]
    #26201360 -

If you have contamination, you shouldn't be using that plate on your grains.  Nothing wrong with using a whole plate to a jar if you have a clean culture.  People have been doing the tiger drop for years now with no problem.


--------------------
Loneliness is a term used by those that aren't comfortable with themselves
Once one conquers loneliness, others are never needed to find happiness

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: in_dot]
    #26201378 -

in_dot said:
If you have contamination, you shouldn't be using that plate on your grains.  Nothing wrong with using a whole plate to a jar if you have a clean culture.  People have been doing the tiger drop for years now with no problem.


No no youre definitely right

OP, dont take what im saying as its okay to use a plate with contamination on it. If it is visibly contaminated ANYWHERE on the plate, its a toss. Or try to clean it.

What i was trying to say was, if there is anything wrong on the plate, it will be exacerbated by grain. I have always heard to use as little of the plate as possible, but maybe that's outdated?


--------------------
REPENT

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: in_dot]
    #26201383 -

If your plate is clean use as much or as little as you want, I usually cut a cross then a circle about 1-2cm away from the edge. Sometimes I make a quick cut to remove the donor agar, this gives me 4 big wedges and I use anywhere from 1-4 per jar depending on how many jars I need to make, usually 2 because I like to have a backup.

If your plate aint clean then dont use it.

A larger wedge will colonize quicker and faster colonization usually means less chance for contamination


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26201385 -

insutama said:
So im getting close to transferring my agar plates to grain and im just wondering what would be better a whole bunch of small pieces of agar or 3 or 4 bigger pieces im gonna have enough plates to put one plate per jar so i could make lots of agar wedges for the jars and speed up colonization also should i shake the jars immediately after putting agar wedges in or just leave them on top of grain until they start colonizing then shake the jars


The way I like to do It,
1 agar puck to a grain jar.
Chop the puck into 20 to 30 pieces in the jar.
Put lid on jar and shake lightly until agar pieces are distribute evenly..


--------------------
     

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: jbgtaa]
    #26201389 -

jbgtaa said:
I have always heard to use as little of the plate as possible, but maybe that's outdated?


Use as little of the plate for a transfer normally, for the reasons you mentioned in your previous comment.  Leaving behind possible contaminants that aren't readily apparent or maybe aren't so obvious to a more novice grower. Also it's important to take a small transfer wedge if you're trying to minimize genetics, such as going for an isolate, although that isn't exactly applicable to this situation. 

However, when you go to grain you should be sure that whatever piece you are using (whether it a wedge or a whole plate) is 100% clean.  If not, or if you're unsure about it, go ahead and take at least one more transfer to a clean plate just for safe measure.  You can take a smaller wedge to grain, but it will take ages to colonize, which can cause problems on its own.  Like I said, I like to take 1/3 to 1/2 of a plate or so.  Sometimes smaller, sometimes larger.  While I'm not a huge fan of putting a whole plate to a jar, it is certainly possible and many people here do it with great success.


--------------------
Loneliness is a term used by those that aren't comfortable with themselves
Once one conquers loneliness, others are never needed to find happiness

Edited by in_dot (09/21/19 03:09 PM)

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: in_dot]
    #26201391 -

in_dot said:
Use as little of the plate for a transfer normally, for the reasons you mentioned in your previous comment.  Leaving behind possible contaminants that aren't readily apparent or maybe aren't so obvious to a more novice grower. Also it's important to take a small transfer wedge if you're trying to minimize genetics, such as going for an isolate, although that isn't exactly applicable to this situation. 

However, when you go to grain you should be sure that whatever piece you are using (whether it a wedge or a whole plate) is 100% clean.  If not, or if you're unsure about it, go ahead and take at least one more transfer to a clean plate just for safe measure.  You can take a smaller wedge to grain, but it will take ages to colonize, which can cause problems on its own.  Like I said, I like to take 1/3 to 1/2 of a plate or so.  Sometimes smaller, sometimes larger.  While I'm not a huge fan of putting a whole plate to a jar, it is certainly possible and many people here do it with great success.


:whathesaid:


--------------------
REPENT

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: jbgtaa]
    #26201650 -

okay to clear things up my plates are 100% clean and they are in standard 100mm petri dishes on the third transfer from the original ms plate
I think ill make my plates into about 6 wedges each put them in the jars and shake to break mycelium up. I like the idea of chopping it up in the jar however it seems that may be bad because then i would have to put the handle of the scalpel into the jar and the handle is not sterilized just sanitized with alcohol

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26201801 -

I'm a believer that the more culture you add the faster your jars will colonize. No brainier..
The faster they colonize the healthier the jar will be, that's If the culture is clean.., obviously..

I have a method that works for me, using plastic table spoons to transfer the culture from the dish to the grain jar and use the plastic spoon to chop up the puck. I do the process in fifteen second with the lid back on the jar.. I do 10 jars just about ever other day in the morning while I have my first cup of coffee. I use 10 spoons to do 10 jars.. No messing around torching scalpels and cooling them down.. The spoons are seven inches long. I tilt the jar so my hand isn't over the jar. When I drop the agar puck in the jar It only two inches from the top of the jar.

It's a unorthodox method that works for me.


--------------------
     

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: micelio]
    #26201810 -

interesting.


--------------------

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: SawCeeJack]
    #26201829 -

How do you sterilize a plastic spoon?


--------------------
Loneliness is a term used by those that aren't comfortable with themselves
Once one conquers loneliness, others are never needed to find happiness

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: SawCeeJack] * 1
    #26201835 -

One plate per jars? That seems like a waste. I'd do 4 jars per plate at least. Putting in more than one wedge isn't going to do much to speed things up because one wedge will colonize all the surrounding grains and you quickly have plenty of inoculation points for when you shake it up. Multiple transfers to a single jar is multiple opportunities to contaminate things.

Also I usually shake it lightly so some grains cover the entire piece. I wouldn't shake it too much though because the wedges will get stuck around the lid sometimes where they don't do any good.

Edited by Kizzle (09/21/19 08:31 PM)

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: Kizzle]
    #26201840 -

Ive only ever used one piece.  I figure opening that jar more than once outweighs any benefit from multiple wedges.

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: in_dot]
    #26201866 -

in_dot said:
How do you sterilize a plastic spoon?


If it's polypropylene, a PC cycle.

If it's polystyrene like disposable cutlery, you don't.


--------------------
"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: in_dot]
    #26201875 -

This Is my routine:
I take a unopened bag and open It in the flow.
I pull ten spoons out and place them on a soak paper towel of alcohol.
I spay each spoon down with alc. Then cover with another soaked paper towel until time of use.
So I can't say I'm sterilizing them.


--------------------
     

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: Kizzle]
    #26201892 -

Kizzle said:
One plate per jars? That seems like a waste. I'd do 4 jars per plate at least. Putting in more than one wedge isn't going to do much to speed things up because one wedge will colonize all the surrounding grains and you quickly have plenty of inoculation points for when you shake it up. Multiple transfers to a single jar is multiple opportunities to contaminate things.

Also I usually shake it lightly so some grains cover the entire piece. I wouldn't shake it too much though because the wedges will get stuck around the lid sometimes where they don't do any good.


Where did I say that?  One plate per jar IS a waste.  Maybe you're getting the words "plate" and "jar" mixed up.


--------------------

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: SawCeeJack]
    #26202283 -

i only have 4 jars to knock up and i have 4 petris so thats why im using a whole plate per jar also this is kinda a crap shoot for genetics my plan is to take a clone of a nice cluster once i get these 4 shoeboxes going then ill make more than 4 jars

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26203040 -

so i did my transfers and the jars that i decided to shake, the agar just stuck to the side of the jar and no matter how much i shook the jars they wouldn't budge so thats rather annoying however im sure the grains knocked off some mycelium from the agar anyway only time will tell now.

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26203057 -

That happens sometimes. I find that if I just keep shaking, really hard they eventually move. Also do side-to-side & up-down shaking.

The pieces of agar tend to move relative to how you're holding the jar while shaking.

Also, if you keep shaking very hard the agar wedge will tear up into smaller pieces which create more innoc points

Edited by pixelpopper (09/22/19 02:53 PM)

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26203142 -

insutama said:
so i did my transfers and the jars that i decided to shake, the agar just stuck to the side of the jar and no matter how much i shook the jars they wouldn't budge so thats rather annoying however im sure the grains knocked off some mycelium from the agar anyway only time will tell now.


I like my agar to be slightly on the hard side..
It chops up nice an no sticking to jar lids and side.

Agar will loose Its jell effect overtime and you'll need to add more agar to get the same effect as a new batch.
Check the exploration date. After a year you'll need to add more to your recipe..


--------------------
     

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: micelio]
    #26203290 -

my agar must be super sticky because i have shaken as hard as i can for like 10 minutes straight and non of them are budging maybe i used to much agar

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26203395 -

Not enough agar.. what's the date on your agar container..?


--------------------
     

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26203400 -

Maybe your grain Is on the wet side..
I hardly ever see allot of moisture in my grain jars (oats)


--------------------
     

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: micelio]
    #26203427 -

i just bought the agar i already threw the package out it was the telephone brand agar
i think my rye is dry enough i let it steam dry then after the steam stopped i had a fan blowing on it for a few hours felt dry to the touch and no moisture in the jars

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26203448 -

how many wedges can you get from an agar plate

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: viper47]
    #26203474 -

depends on size of wedges lol

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26203488 -

i turned my plates into 6 wedges

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26203692 -

insutama said:
so i did my transfers and the jars that i decided to shake, the agar just stuck to the side of the jar and no matter how much i shook the jars they wouldn't budge so thats rather annoying however im sure the grains knocked off some mycelium from the agar anyway only time will tell now.


I did try to warn you :grin:

If you want inoculation points, a larger wedge will touch more grains which will create more inoculation points sooner. No need to try and break the agar apart or spread it around, the grains will do that when you shake it later. Just get it so the colonized side is touching the grains.


--------------------

Edited by Kizzle (09/22/19 09:21 PM)

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: Kizzle]
    #26203735 -

if you flip the jar end over end a couple times slowly (instead of shaking) the wedge usually ends up around the bottom of the jar.


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: Kizzle]
    #26203768 -

Kizzle said:
insutama said:
so i did my transfers and the jars that i decided to shake, the agar just stuck to the side of the jar and no matter how much i shook the jars they wouldn't budge so thats rather annoying however im sure the grains knocked off some mycelium from the agar anyway only time will tell now.


I did try to warn you :grin:

If you want inoculation points, a larger wedge will touch more grains which will create more inoculation points sooner. No need to try and break the agar apart or spread it around, the grains will do that when you shake it later. Just get it so the colonized side is touching the grains.


lol i should have listened i only shook 2 of the 4 jars so at least there not all stuck on the sides of the glass
it looks like all the mycelium is off the agar that is stuck to the glass from shaking the jar so much hopefully it will inoculate the grains

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: AyePlus]
    #26203769 -

AyePlus said:
if you flip the jar end over end a couple times slowly (instead of shaking) the wedge usually ends up around the bottom of the jar.


i tried shaking the jars up and down and side to side and just flipping the jar nothing got them to move so now im just gonna let them sit there is still one or two agar slices on top of the grain and like 4 stuck to the glass so ill just wait till the grains start getting colonized then ill shake it

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: insutama]
    #26203772 -

Yeah, and I don't think it's really a matter of the agar being too sticky as it is the force of the shake pressing it against the glass that makes it stick. The best way to get it off if it sticks it to gently roll some grains over it. If you use too much force it can really get stuck.


--------------------

Extras: Filter Print Post Top
Re: Big or small agar wedges to j [Re: Kizzle]
    #26203831 -

Yeah once stuck it's stuck most of the time, you can try slapping the opposite side of the jar, every once in a while that helps but if you gently roll end over end from the beginning it works great.


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
πŸ…‚ πŸ„° πŸ„Ό πŸ„΄  πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

Extras: Filter Print Post Top
Re: Big or small agar wedges to j [Re: AyePlus]
    #26204102 -

good to know for next time thanks

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: AyePlus]
    #26204404 -

AyePlus said:
if you flip the jar end over end a couple times slowly (instead of shaking) the wedge usually ends up around the bottom of the jar.


:whathesaid:
Heres a diagram I posted a while back for visual aid on how to get the agar wedge were ya want it in the jar in no time.


--------------------
-The heaviest thing one will ever carry is a thought-
-"Like a Blind man In an orgy you gotta feel things out.".-
-When we agree about our hallucinations, we call it β€œreality".-
-If you defy authority because your told to, that's no better than blindly trusting authority.-

psychonautwiki.org

How it should & shouldn't look - NEW CULTIVATORS GUIDE
BOD's Easy AF OAT prep tek.
Principles of mushroom growing for beginners

Extras: Filter Print Post Top
Re: Big or small agar wedges to jars [Re: R.I.P.Zappa]
    #26204751 -

instresting i think either my wedges were to big or my agar was exceptionally sticky either way im just leaving the jars for now i dont want to keep shaking them before they even start colonizing

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3  [ show all ]

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Innoculating with agar wedge observations homebrew 3,778 4 03/01/02 08:00 AM
by homebrew
* Innoculation with agar wedge Visigoth 5,895 6 01/15/23 12:59 PM
by Screwup
* Is this healthy? Current concerns. PF Tek Jars frenzylock 5,236 17 05/06/21 05:44 PM
by Ashtray161
* Re: mold in agar plate Anonymous 14,231 3 12/04/99 12:35 AM
by Anonymous
* WBS jar stalling after agar inoculation (photo) Josborne 1,251 14 05/04/21 02:29 PM
by Stipe-n Cap
* 18 Jars no colonization WHAT GIVES SumGuy 1,725 6 10/23/01 11:35 AM
by SumGuy
* Agar Questions - Fluffy cloud like Growth indicaz 2,129 9 01/30/03 01:01 PM
by Anonymous
* Post deleted by MOE THE MAD SCIENTIST
( 1 2 all )		 vatoloco 10,419 21 04/30/02 03:27 AM
by Zebedee

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,301 topic views. 8 members, 707 guests and 74 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.052 seconds spending 0.012 seconds on 16 queries.