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InvisibleStipe-n CapMDiscord
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Pastywhyte]
    #26191109 - 09/16/19 07:33 AM (4 years, 5 months ago)

Alan Watts, karma:



His voice is my go to antidepressant.

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Offlinejbgtaa
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: DnDRnD]
    #26191247 - 09/16/19 08:55 AM (4 years, 5 months ago)

Quote:

DnDRnD said:
I confess everytime I have extra agar i never know what to start up, it literally takes me a good 10-20 minutes to decide what varieties or species I wanna start up  :shrug: decided to do moby dick, more PE6, and galindoli this time




Man i tried this and ended up with a ton of germ plates that i couldnt keep straight for the LIFE OF ME. no matter how precise the labeling and documentation, i fucking threw out any germ plate that wasnt showing isolation past my decided point... Was left with like 3 :lol:


--------------------
If the thunder don't get ya, the lightning will.
In another time's forgotten space, your eyes looked through your mother's face.
Trade List
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InvisibleTormatoMFacebookDiscordReddit
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Registered: 07/01/17
Posts: 6,067
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Pastywhyte]
    #26192015 - 09/16/19 03:04 PM (4 years, 5 months ago)

Quote:

Pastywhyte said:
Karma is the weight of your unfulfilled desire that pulls your soul back from the astral plane to the gross physical realm.



Objective being to have no karma :strokebeard2:

I like your style Pasty :zaphod:


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OfflineTweeq
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Tormato]
    #26193173 - 09/17/19 03:57 AM (4 years, 5 months ago)

I confess, I am kinda disappointed about our enigma culture putting out regular cubes..

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OfflineFailboat
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Tweeq]
    #26193489 - 09/17/19 09:42 AM (4 years, 5 months ago)

I confess I have mold trouble still. Got quite a haul to throw away today. My sterile technique is just awful. Mostly G2G sloppiness. Gonna do some cleaning today.

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OfflineDnDRnD
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: jbgtaa]
    #26193513 - 09/17/19 09:53 AM (4 years, 5 months ago)

Quote:

jbgtaa said:
Quote:

DnDRnD said:
I confess everytime I have extra agar i never know what to start up, it literally takes me a good 10-20 minutes to decide what varieties or species I wanna start up  :shrug: decided to do moby dick, more PE6, and galindoli this time




Man i tried this and ended up with a ton of germ plates that i couldnt keep straight for the LIFE OF ME. no matter how precise the labeling and documentation, i fucking threw out any germ plate that wasnt showing isolation past my decided point... Was left with like 3 :lol:




Oh god I totally understand that! Lmao I have ALOT of prints that are dirty as all hell, like I swabbed some AA+ spores to agar and only 2/4 plates are not contaminated, but they have no good growth either

The other 2 got mold so quick the spores never had a chance to germinate

Literally atleast half my plates end up so dirty I can't even find where to begin cleaning it up :rofl2: the trick ive found that helps is to start up as many plates of one kind as you can lmao

Its funny with trading prints around, sometimes I get a print thats pretty much just mold spores and then sometimes you get a super super clean print that would almost feel safe enough to put straight to grain :shrug: theres never a middle ground it seems like


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662

Down with the bourgeoisie and up with the proletariat

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OfflineShaperDreaming
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: DnDRnD]
    #26193710 - 09/17/19 11:30 AM (4 years, 5 months ago)

Confession 1: Some family medical shit has kept me from doing all the work I want to do... and I really need to scale back my work for a while :frown: I'm trying to figure out what will be best, but for the next 3-4 months I'll need to head out to a city 5 hours away every other week for a few days at a time. It just makes the mycology work both overwhelming (since I need down time too) and it's all so spaced out.

Confession 2: I really don't like rye... I feel bad for saying this! Everyone is like "Rye's top-tier spawn food!" and... I can't get anything but over-cooked grains OR contams (they're not happening on other grains, so it's the rye... and it's likely from not being saturated enough when cooked, which fine, whatever). I was having some minor problems with oats, and bought both rye and millet. Millet has been nothing but basically perfect... so I'm going to just use that? :shrug:

Confession 3: All my agar plates are totally covered and I haven't transferred them yet :uhoh: Now I'm paranoid and this will likely cause me more work since I'll want to clean them up an extra couple of transfers to feel better. :facepalm3:

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Offlinetryptkaloids
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #26193727 - 09/17/19 11:39 AM (4 years, 5 months ago)

Meh, use em


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
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OfflineShaperDreaming
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: tryptkaloids]
    #26193729 - 09/17/19 11:40 AM (4 years, 5 months ago)

You make it sound... so simple

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Offlineverum subsequentis
seeker of truth
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #26193730 - 09/17/19 11:41 AM (4 years, 5 months ago)

Just take one transfer from.... Not the edge, if you're worried about it. It usually works out fine. If the culture was clean, in my experience, it usually still is.

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OfflineShaperDreaming
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: verum subsequentis]
    #26193745 - 09/17/19 11:51 AM (4 years, 5 months ago)

True, true. I've just had so many issues lately that I'm extra paranoid right now. I've done transfers from like the 2/3 out position before when I go back and it's usually fine. I store them in my "lab" with my flow hood anyways, it's probably the cleanest room in my house (also, like saran wrap etc).

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InvisibleSunnyDayze
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Registered: 12/30/17
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: verum subsequentis]
    #26193813 - 09/17/19 12:21 PM (4 years, 5 months ago)

Quote:

verum subsequentis said:
Just take one transfer from.... Not the edge, if you're worried about it. It usually works out fine. If the culture was clean, in my experience, it usually still is.




:whathesaid:

I use plates that go to the edge, don't overthink it unless they are spilling out over the edges I guess lol  I've only seen gourmet do this, never had cubes do it, not saying they can't.

Best of luck girl, you have so much shit on your plate try not to sweat the small stuff.

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OfflineShaperDreaming
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: SunnyDayze]
    #26193834 - 09/17/19 12:29 PM (4 years, 5 months ago)

Thanks :heart: Also, yeah, I've had oysters that take over all the open space in plate before! Wild shit, even tried to climb out of the petri dish.

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OfflineDnDRnD
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: ShaperDreaming]
    #26193867 - 09/17/19 12:47 PM (4 years, 5 months ago)

Quote:

ShaperDreaming said:
Thanks :heart: Also, yeah, I've had oysters that take over all the open space in plate before! Wild shit, even tried to climb out of the petri dish.




I had one of my KO's do that in a no pour,  I still put it to grains :shrug: I don't think I've seen any cubes climb up the sides yet

But as long as the culture was clean it should be good :thumbup:


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662

Down with the bourgeoisie and up with the proletariat

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InvisibleCaps McGee
Grandaddy Smurfshack
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 14,357
Loc: ally known as ... Flag
Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: DnDRnD]
    #26193893 - 09/17/19 01:10 PM (4 years, 5 months ago)

May be hippie jargin, but I absolutely believe that your energy has more an affect than you think.. I see a lot of guys taking crazy precaution, and continue to have issues: and I wholeheartedly believe ONLY bc they expect/worry it to fail... Which brings me to my first confession...

Outside of dipping my scalpel for flaming, I've not touched a bottle of alcohol for WEEKS.. Not even sure the jar I place my tools into is even alcohol anymore:Was 70%, and left open more than once... May try plain water, or even dry flaming... I'm on this kick about putting forth as little effort as possible without diminished results lmao... :shrug: we all have our thing I suppose...

Confession two... Not sure why, but I'd been having issues getting logged in from my computer since the site was hacked: not sure what I was doing wrong, but received a link to a place to reset cookies or some shit, and long story short I'M IN... No more excuses, so will be posting tek series in full(or parts) in the next few days... Species specific gourmet teks are not ready yet, But are actively being worked on around everything else around here lol... Right now though; agar checks!

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Offlineverum subsequentis
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
    #26193902 - 09/17/19 01:16 PM (4 years, 5 months ago)

i always only flame my scalpel. never ever dip it in iso. i clean it up with an iso soaked paper towel and then it's all flame

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InvisibleCaps McGee
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Registered: 10/28/17
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: verum subsequentis]
    #26193906 - 09/17/19 01:21 PM (4 years, 5 months ago)

BETTY WHITE IN A MASERATI THEN!!!

No more alcohol... CHECK!

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Offlineverum subsequentis
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
    #26193909 - 09/17/19 01:22 PM (4 years, 5 months ago)

to be clear. I use iso a lot. Just not for that

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InvisibleCaps McGee
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Registered: 10/28/17
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: verum subsequentis]
    #26193913 - 09/17/19 01:24 PM (4 years, 5 months ago)

Well it's all I'm STILL using it for... So should work out without lol... thanks mane!

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OfflineShaperDreaming
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Re: TRUE CONFESSIONS - of an amateur mycologist *DELETED* [Re: Caps McGee]
    #26194005 - 09/17/19 02:23 PM (4 years, 5 months ago)

Quote:

verum subsequentis said:
never ever dip it in iso.




I used to dip ... until I switched to a propane torch for my flaming. I was using disposable scalpels at the time, which have plastic handles. It totally lit on fire and started melting very quickly, and I ALMOST DOUSED IT IN THE LITTLE CUP OF ISO. I'm not sure how I stopped myself from doing it in time, but from that point on I decided I shouldn't have a container of ISO open around a very hot flame ever again.

Now my main issue with scalpels is how to not get it so hot because I started using a hobby/exacto knife and the shaft is full metal. I'm going to either wrap it in something or get multiples...

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