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Offlineinsutama
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Agar T2 pics advice needed
    #26190348 - 09/15/19 08:17 PM (1 year, 5 months ago)

Once again looking for some of your guys advice on these plates these are all the second transfers from a ms plate.
Ill start from best to worst first i have 2 PE plates these look amazing i dont see no contams

Plate #1

Plate #2


Next I have 2 B+ plates and im pretty sure these look good also however in the center of the mycellium it looks a little weird i dont know if this is normal or not would love your opinion on this one

Plate #1

(This is the one im unsure about the clearer ring around where the original transfer was)

Plate #2

(This one i think is good there is 2 satellite colonies from when the agar wedge moved after i transferred and had to tap dish to get the agar back to center of dish)

Now for the BAD

First 2 plates are Golden teacher and im almost positive there is bacteria contam please let me know if im wrong.
Plate #1

(From the 12-4 clock range is where im worried)

Plate #2

(This one is the same area im worried about 12-5)

And lastly the worst plates which are both Mazatapec im 100% sure these are contaminated but thought i would get your guys opinion before i toss them
Plate #1

(This one has the ring like i was talking about on the first GT picture plus looks like bacteria from 3-7)

Plate #2

(Not much to say about this one looks like all bacteria around it all)


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Invisiblerickyswamps
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Re: Agar T2 pics advice needed [Re: insutama]
    #26190481 - 09/15/19 09:44 PM (1 year, 5 months ago)

I don't really see bacterial contam, could just be weak growth.  I'd transfer from a good spot and see how they look after that.


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Offlineinsutama
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Re: Agar T2 pics advice needed [Re: rickyswamps]
    #26190576 - 09/15/19 10:58 PM (1 year, 5 months ago)

on the last two pics of my MAZ isnt that outer edge bacterial contam ?


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OfflinemushboyM
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Re: Agar T2 pics advice needed [Re: insutama]
    #26190593 - 09/15/19 11:06 PM (1 year, 5 months ago)

Take the pics at an angle like this.

That way you don't get reflections:thumbup:

Your good pics look good. I'd toss the bad ones. They could be ok after some transfers but I'd rather go back to spores.


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Offlineinsutama
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Re: Agar T2 pics advice needed [Re: mushboy]
    #26190627 - 09/15/19 11:26 PM (1 year, 5 months ago)

ya thats what im thinking also i have enough good ones to make more than enough jars for now and ill try taking pictures on a angle and see if that helps and maybe post them on here tomorrow thanks for the tip. Would keeping a bad plate in the same container as a good plate cause cross contamination ? there sealed with parafilm


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OfflinemushboyM
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Re: Agar T2 pics advice needed [Re: insutama]
    #26190644 - 09/15/19 11:37 PM (1 year, 5 months ago)

Nah

I mean if they look like this?

Burn with fire

keep them away from healthy plates but some bacteria or dirty cultures? Nah.


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Offlineinsutama
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Re: Agar T2 pics advice needed [Re: mushboy]
    #26191038 - 09/16/19 08:10 AM (1 year, 5 months ago)

okay cool thanks


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Offlineinsutama
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Re: Agar T2 pics advice needed [Re: insutama]
    #26191522 - 09/16/19 01:40 PM (1 year, 5 months ago)

Here is a better close up of the plates that were questionable i tossed them all now and took one transfer from the GT plate to see if it will come back clean.

GT PICS



MAZ PICS






Whats your opinions on these plates ?



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OfflineBorisTBD
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Re: Agar T2 pics advice needed [Re: insutama]
    #26364318 - 12/06/19 07:52 AM (1 year, 2 months ago)

Just wondering if you made a transfer from that maz plate?  If you took any pictures that I might be able to compare my maz plates to? I am currently started maz spores to agar and it looks very similar but with less rhizo’s. Much appreciated.


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