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Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Psonic] * 1
    #26214898 -

well sunlight is partially blue light.. so i guess sunlight is right for growing.

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Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: AlienSapien]
    #26228597 -

So I should use a LED bulb with 6500K.

I have a 5000K LED, but the 6500K lights have more blue, so will probably produce more psilocin.

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Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: AlienSapien]
    #26228640 -

I would question this before running out and dropping money on lights, but what the hell! Post your results...

https://www.lightbulbs-direct.com/article/choosing-ultraviolet-bulbs/

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Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: AlienSapien]
    #26228861 -

AlienSapien said:
So I should use a LED bulb with 6500K.

I have a 5000K LED, but the 6500K lights have more blue, so will probably produce more psilocin.


It wont make a lick of fucking difference lol


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Welcome to the Advanced Mycology Forum

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Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: bodhisatta] * 1
    #26229533 -

https://www.academia.edu/26860088/Enhancement_of_Indole_Alkaloids_Produced_by_Psilocybe_cubensis_Earle_Singer_Agaricomycetideae_in_Controlled_Harvesting_Light_Conditions


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Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: vinnie boombotz]
    #26230368 -

vinnie boombotz said:
https://www.academia.edu/26860088/Enhancement_of_Indole_Alkaloids_Produced_by_Psilocybe_cubensis_Earle_Singer_Agaricomycetideae_in_Controlled_Harvesting_Light_Conditions




Full version with out logging in -> https://www.researchgate.net/publication/247855008_Enhancement_of_Indole_Alkaloids_Produced_by_Psilocybe_cubensis_Earle_Singer_Agaricomycetideae_in_Controlled_Harvesting_Light_Conditions


Abstract
Different variables including species, strain, glucose and ammonium succinate concentration in
the growth medium, pH, temperature, timing and oxidation have been accounted for the Psilocin
(PC) and Psilocybin (PB) content in the "Magic Mushrooms"(MMs). These are but some of the
variables in a constellation of factors that complicate consistency in the production of PC and PB.
In an attempt to study the effect of light on chemical constitutions, some samples were kept in
dark, some samples were kept in dim lighting, whereas others were exposed to natural but indirect
light. After picking and drying Mushrooms, a simple one-step extraction involving
homogenization of the dried fruit bodies of fungi in chloroform and derivatization with MSTFA
was performed. The samples were then analyzed by gas chromatography–mass spectrometry. This
investigation shows that the relative PC and PB content of the mushrooms is highly dependent on
the light condition. This variation could amount to 100 fold of active components (PB and PC) in
samples harvested in dark condition compared to the samples harvested in indirect light condition.
Therefore it can be concluded that UV light may have destructive effect on the active components,
which would readily explain why sun-struck collections are less potent

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Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: murderlabz] * 1
    #26279243 -

Using blue light is supposed to help a lot of species in certain stages of growth anyway right, i thought this has been tested repeatedly (some species do prefer other wavelengths like reds apparently).
And what color was it that stimulates production of cordycepin in Cordyceps compared to other light?

If light factors in it's not a "catalyst", but there may still be a correlation about certain metabolic processes or growth development and their induction by light as a trigger... The biosynthesis of relevant alkaloids may very well be indirectly dependent on those metabolic processes, but it wouldn't necessarily show up as a known critical factor in literature.

Obviously we like to see more evidence etc but it's not at all implausible. This was in a patent right, so how is it substantiated?

AFAIK his mother was believed to bounce back much quicker from radiation wiping out her immune system, from turkey tail stimulating the immune system. Something which apparently many mushrooms may do because or compounds rather common in them (tho those are not created equal). Not the same as curing cancer, but I'm sure he blew it out of proportion talking about it here and there.

People say 'at least he knows how to sell it all'.. :\  trust me I am skeptical about the dude too and it feels more like shilling or something. I'm also fine believing that he likes to take credit for other ppl's work in some way.

But not sure it's such a good idea to be biased about every concept that came from his direction. If its bad, just take it apart for reasons. If he doesn't offer nuance or fairness, maybe that's what we ought to try and do.

Anyway I think he is playing a tricky game. Someone might argue that it helps get fungi good publicity, but if he oversells certain badly founded claims and there is backlash it may harm the reputation of the field of research.

But when it comes to scientific claims let's stick to scientific validation or dismissal.


--------------------
My trade lists:
  • Fungi - Edible / Gourmet
  • Fungi - Medicinal/Insecticides/Bioluminescents
  • Fungi - Actives
  • Fungi - Old Exotic Spores (Actives)
  • Fungi - Misc Hunted Mushroom Spores
  • Plants
  • Other items - Supplies etc.


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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Solipsis]
        #26280038 -

    I run blue light and get good results. It’s not always 12 hour cycling...this amount isn’t 100% needed, but it’s not going to hurt anything to mimic natural patterns of sunlight like that either. Substrate sort of just pins when it pins; a drop in temperature, increase in evaporation, some type of light source all seem to help do the trick if full colonization is reached. I’m sort of stating the obvious here, but still.


    --------------------
    ‎שלום וְאור | PEACE & LIGHT

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Psilotyl] * 1
        #26283284 -

    Some supposed studies suggest that milliseconds is all it takes. I honestly am not sure how we get this 12/12 cycle.

    Blue light will work even if it's just a part of the cool daylight spectrum which otherwise may be used. When i got into blue it was because it lacks the unnecessary wavelengths so is just a fraction more sustainable, i didnt know how many other's caught on to the idea. Or how fucked up you get from looking into it.
    So honestly I consider it a novelty now, unless systems are implemented to change the lighting depending on the presence of an employee/worker.

    Just like some other things like the use of an incubator, at a very small scale it gets kind of like a novelty or concept.. or just kind of silly or experimental. While if upscaled there can very well be a point and it may be more worth it.

    IMH0.02


    --------------------
    My trade lists:
  • Fungi - Edible / Gourmet
  • Fungi - Medicinal/Insecticides/Bioluminescents
  • Fungi - Actives
  • Fungi - Old Exotic Spores (Actives)
  • Fungi - Misc Hunted Mushroom Spores
  • Plants
  • Other items - Supplies etc.


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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Psonic] * 2
        #26310816 -

    Psonic said:
    From personal experience having a family member (my dad) that started using Pauls products (not sure which one) after being on radiation for a few weeks and was told about it from a fellow patient (not as a cure) it doesnt seem to be snake oil really imho. His antibodies and other immunity factors had improved dramatically along with his symptom tolerance just a couple dr visits after starting it for its natural anti biotic properties. Even his dr said that polysaccharides in mushrooms can help white blood cells and overall immunity And i don't think Paul ever said it cured her cancer but that it saved her life by boosting her immune system that greatly needed improving as she was undergoing conventional cancer that people very rarely recover from such a chronic stage. Plus the research hes doing with the bees is super solid and beneficial and from what i here is promising and going to be released to the public to help apieries for free.... I love Paul lol sorry


    There are no actual mushrooms in Paul's supplements, they're based on grain/rice/mycelium biomass and contain mostly starch.

    Just in case you don't believe what I say about it. Take it from Paul Stamets:

    Here is an excerpt from one of Stamets’ patents:

    EXAMPLE 9 [00123] The medicinal mushroom mycelium is grown utilizing liquid culture techniques. Whereas growing on rice might have 30-40% conversion of rice to mycelium, liquid vat culture may have essentially complete conversion with >3x more mycelium per unit mass.

    30-40% conversion of rice to mycelium. Meaning 60-70% rice.

    Check this research that confirms that 100%.

    High alpha-glucan = starch which is from grain. While they will tell you the mycelium consumes the majority of the grain, the actual test data tells an entirely different story.

    That is the reason they do not share actual lab test reports of their products. The same with Paul Stamets's products. Lot's of talk, but zero facts.

    Their products are also not extracted, meaning the bioavailability is unpredictable for 80% of people.

    Summarising: you are paying top dollars for useless starch.

    IF you want to buy REAL mushroom supplements for your dad, I've only found 2 companies that actually SHARES! lab results. Very low starch content and high beta-glucans. They tell you the % of Beta-glucans. This is the second company! they use only fruiting bodies and they tell you exactly how much starch content there is in their products. Although, fruiting bodies are not where the most medicinal content is but is certainly better than Paul's products with 80% starch content or more.

    Here is a quote from Oriveda.

    Quote:
    This information can be found on the product page for each mushroom. In some online communities the general idea is circulating that fruiting body is always preferable over mycelium. This is wrong.

    In brief, here's why (it's mainly just common sense !):
    for some mushrooms the most important active ingredients are actually in the mycelium (Lion’s Mane (erinacines), Turkey Tail (PSP/PSK), Cordyceps CS-4 (no fruiting body extracts exist!))
    there is a difference between mycelium-on-grains/rice (biomass) and pure mycelium. Biomass should always be avoided as it is contaminated with undigested substrate in the form of starch. This includes all US-cultivated products. We only use pure mycelium, free from starch, if the products properties request the use of mycelium
    quality claims are only worth something if they are backed with actual test reports. This includes a claim like 'fruiting body extracts are better than mycelium-based extracts' or vice versa. Nice statement, but where is the proof ? Also see our 'I'm confused by all the information online' entry, above. Using ‘we use fruiting bodies only!!’ has the subtext ‘it’s better!’ but actually only reveals the ignorance of the vendor, and the actual specifications, if any (% of glucans, terpenes etc.) usually have no verifiable source or backup in the form of official documents. Only specifying active ingredients is acceptable as proof of superior quality.


    Going back to fruiting bodies vs pure Mycelium for medicinal content.

    Research has found that pure mycelium has most of the medicinal content! Companies like Oriveda uses a method called deep layer cultivate mycelia which is basically what we call "liquid culture" but they grow pure mycelium in MASSIVE amounts and extracts the medicinal content from there. NOT GRAINS. Companies like Paul and Aloha medicinals just want to sell you starch, is a FACT. They only care about money, not curing your mom or dad, if they would care they would share LAB TEST RESULTS.


    --------------------

    My Perfect wheat preparation TEK
    My holy grail no pour agar tek

    Edited by Mr. Alien (11/10/19 07:27 AM)

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Mr. Alien]
        #26311275 -

    Also rice is full of arsenic. I wonder if Paul's snake oil extracts end up concentrating that common contaminant of rice.


    --------------------
    Welcome to the Advanced Mycology Forum

    Bod's Library    Article behind a paywall? I can try to get it for you

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: bodhisatta]
        #26311339 -

    He's a great salesman. Now he's making movies about how fantastic the world of fungi is! He knows his shit but he also knows how to make money. When he said he cured his mother, you think he gave her starch?


    --------------------

    My Perfect wheat preparation TEK
    My holy grail no pour agar tek

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Mr. Alien]
        #26334622 -

    So what exactly are the differences between the fruit bodies and the mycelium? Would psilocin and psilocybin grow in mycelium as well as the fruit bodies, or is it only produced at maturity?

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: mushman1017]
        #26334906 -

    They are also produced in the mycelium but a little less of it. Depending on the species though, it can apparently be viable to extract the mycelium for the alkaloids, possibly worthwhile.
    I kinda want to assume the mycelium reacts the same way to light where growing in darkness gives more psilocin and growing in light more psilocybin relatively (they would be converted into each other, im not talking about a change in overall potency).

    But my question would be: how local is this effect? Are these concentrations (proportions) changed throughout the mycelium - even the bottom dark parts - if the above ground parts exposed to light are receiving photoperiod triggers?


    --------------------
    My trade lists:
  • Fungi - Edible / Gourmet
  • Fungi - Medicinal/Insecticides/Bioluminescents
  • Fungi - Actives
  • Fungi - Old Exotic Spores (Actives)
  • Fungi - Misc Hunted Mushroom Spores
  • Plants
  • Other items - Supplies etc.


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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: mushman1017]
        #26335217 -

    mushman1017 said:
    So what exactly are the differences between the fruit bodies and the mycelium? Would psilocin and psilocybin grow in mycelium as well as the fruit bodies, or is it only produced at maturity?


    As far as i know psilocybin is produced in the mycelium as well... I don't know in what concentration tho... fruiting bodies might have more psilocybin content than the mycelium i don't know but in Medicinal mushrooms like Tukey Tail and Lion Mane the mycelium have much more healing properties. Just don't grow it in grains :P it will result in high starch content.


    --------------------

    My Perfect wheat preparation TEK
    My holy grail no pour agar tek

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Mr. Alien]
        #26337733 -

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3627973/

    here is a controlled experiment

    Color spectrum DOES INDEED effect the chemical makeup of mushroom fruit bodies


    --------------------

    Legal Disclaimer! Everything I post is fictitious and mere role playing game as a scientist.

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Comprehension]
        #26337749 -

    Fine and dandy if you want to grow with one spectrum. Most people just use a fluorescent which has more than enough of any spectrum mushrooms need anyway. Besides that article really focused on correlations to plants which makes me think it was just some people who were grasping for straws to publish another paper to keep money rolling


    --------------------
    Welcome to the Advanced Mycology Forum

    Bod's Library    Article behind a paywall? I can try to get it for you

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: bodhisatta]
        #26337803 -

    I’m not a fan of pauls, however aloha medicinals also claims that mycelium is full of goodies.

    I’ve yet to email aloha for their analysis but I do believe aloha will provide data. Paul’s company will not last I checked, as they believe it is basically inconsistent for the lack of remembering how they put it exactly when I emailed them long ago

    I personally am a fan of fruits and myceliated grain supplements. I’ve eaten supplements and definitely noticed they were cleaning me out. My nose told me. Not to mention a lady who swears they cured her digestive issues to say the least which I’ve met first hand. In fact I recommend she try them.

    To be clear the supplements I was eating when I noticed a difference was ohm brand Paul’s are pricey for sure. Make your own try them out for a month see how feel then come back and report. My conclusions were made long ago. Seems to be a lot of back and forth on this topic and many haven’t even spent $20 on some supplements and tried them lol


    --------------------
    **A Wealth of Cannabis Growing Information Condensed into a Blessing of a Recipe**

    Mushrooms = “Superior Organisms”

    https://www.cosmicreality.net/wsmn-shungite-science-with-walt-silva.html

    Edited by Raven44 (11/22/19 09:50 AM)

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Raven44]
        #26337818 -

    John Holliday is a known liar too.
    Example
    The mushroom that had a smell that gives woman orgasms.... one of his great mycology stories.

    Someone did a study on the components of common mushroom supplements. Paul's did the worst. It's mostly starch. And the polysaccharides in the product not extracted so they're barely bioavailable


    --------------------
    Welcome to the Advanced Mycology Forum

    Bod's Library    Article behind a paywall? I can try to get it for you

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    Re: 280-400 nm blue light to induce production of psilocin, psilocybin and other tryptamines in myc [Re: Mr. Alien]
        #26337843 -

    Mr. Alien said:
    mushman1017 said:
    So what exactly are the differences between the fruit bodies and the mycelium? Would psilocin and psilocybin grow in mycelium as well as the fruit bodies, or is it only produced at maturity?


    As far as i know psilocybin is produced in the mycelium as well... I don't know in what concentration tho... fruiting bodies might have more psilocybin content than the mycelium i don't know but in Medicinal mushrooms like Tukey Tail and Lion Mane the mycelium have much more healing properties. Just don't grow it in grains :P it will result in high starch content.


    So what do you suggest growing them on? Agar plates and just scrape off the myc that grows on the agar?

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