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How long can I wait to pc brf substrate jars?
    #26184874 -

Ive made 28 half pints tonite. Its getting late an I have some errons to run tomorrow. My pc wil hold 14 half pints at one shot. So my plan is to wait till about 2-3:00 pm tomorrow  to finish pc'ing them along with 5 glass petri dishes and agar. Is this too long or did I waste my time? What would be the max time for future reference ?

Edited by StoneBone (09/26/19 08:40 PM)

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Re: How long can I wait to pc brf substrate jars? [Re: StoneBone]
    #26185492 -

First off, assuming you properly follow sterilization procedures in a PC, pressure cooking un-sterilized BRF accomplishes one thing: sterilization.

The pressurized steam transfers heat energy through the glass of the jars and through the material inside the jars.

It takes time for the heat to make it through the glass and into the jars, and the kind of living things floating around in your air will die after prolonged exposure to the kind of heat generated by a pressure cooker, or any steam bath for that matter.

The only difference is how long it takes to bring the jar to a temperature that will accomplish sterilization, and how long you need to hold the jar at that temperature in order to kill the critters/spores inside.

For the sake of argument, assuming you're planning on PC-ing the jars at 15 PSI for 90 minutes, with proper venting, etc... what would still be alive inside the jars?

So, lets think through this...

What could happen if you left un-sterilized BRF jars for a day? They would remain contaminated, right? Worst case, whatever spores or bacteria were floating around in your air might start germinating...

So after a day without sterilizing, what do you have? You've still got un sterilized BRF jars. And what happens when you sterilize un-sterilized BRF jars?


Linking the "Updated PF-Tek" posted by Ziran, for additional reading.
https://www.shroomery.org/forums/showflat.php/Number/24179086/page/3

Also linking this tidbit from 2012, where someone fucked up their BRF jars in a PC. Don't do what this guy did.
https://www.shroomery.org/forums/showflat.php/Number/16391593#16391593

IMO, it's very helpful to not only understand the exact procedures you're doing, but also why you're doing them. Why do you inoculate after PC/Steaming the jars? Why do you need to torch the syringe needle between jars? What's that dry vermiculite layer for? If you can figure out what's going on inside your jars, you will have an easier time using your intuition to guide your grow. Happy growing! I hope I've been helpful. And sorry if I've repeated things you already know. I wanted this post to be helpful to lurkers as well as you.

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Re: How long can I wait to pc brf substrate jars? [Re: GoSledDog]
    #26185499 -

I wouldn't wait more than 4-6 hours. They're so easy to prepare just make the mix before sterilizing

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Re: How long can I wait to pc brf substrate jars? [Re: GoSledDog]
    #26185507 -

GoSledDog said:
First off, assuming you properly follow sterilization procedures in a PC, pressure cooking un-sterilized BRF accomplishes one thing: sterilization.

The pressurized steam transfers heat energy through the glass of the jars and through the material inside the jars.

It takes time for the heat to make it through the glass and into the jars, and the kind of living things floating around in your air will die after prolonged exposure to the kind of heat generated by a pressure cooker, or any steam bath for that matter.

The only difference is how long it takes to bring the jar to a temperature that will accomplish sterilization, and how long you need to hold the jar at that temperature in order to kill the critters/spores inside.

For the sake of argument, assuming you're planning on PC-ing the jars at 15 PSI for 90 minutes, with proper venting, etc... what would still be alive inside the jars?

So, lets think through this...

What could happen if you left un-sterilized BRF jars for a day? They would remain contaminated, right? Worst case, whatever spores or bacteria were floating around in your air might start germinating...

So after a day without sterilizing, what do you have? You've still got un sterilized BRF jars. And what happens when you sterilize un-sterilized BRF jars?


Linking the "Updated PF-Tek" posted by Ziran, for additional reading.
https://www.shroomery.org/forums/showflat.php/Number/24179086/page/3

Also linking this tidbit from 2012, where someone fucked up their BRF jars in a PC. Don't do what this guy did.
https://www.shroomery.org/forums/showflat.php/Number/16391593#16391593

IMO, it's very helpful to not only understand the exact procedures you're doing, but also why you're doing them. Why do you inoculate after PC/Steaming the jars? Why do you need to torch the syringe needle between jars? What's that dry vermiculite layer for? If you can figure out what's going on inside your jars, you will have an easier time using your intuition to guide your grow. Happy growing! I hope I've been helpful. And sorry if I've repeated things you already know. I wanted this post to be helpful to lurkers as well as you.



Awesome post! Following a tek is great, but if you understand whats going on you'll have more success guaranteed.

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