Home | Community | Message Board


North Spore
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Edibles   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   Left Coast Kratom Buy Kratom Capsules, Buy Kratom Extract, Kratom Powder For Sale   Amazon Agar

Jump to first unread post. Pages: 1
Offlinethe_sonic16
Mushy McMusherson
I'm a teapot User Gallery


Registered: 03/10/19
Posts: 396
Loc: Somewhere off topic
Last seen: 30 days, 5 hours
Revitalize slow growing culture?
    #26172628 - 09/06/19 03:45 PM (1 year, 5 months ago)

Ok, so I've had this culture that I've transferred probably 4-5 times over the course of a few months. With each transfer the mycelium seems to grow a little slower. Is there a way to revitalize slow/unhealthy mycelium or is what I'm seeing just senescence?


--------------------
Sonic's "I'll buy a pressure cooker someday" Instant Pot times and settings:
  • Popping Corn Rapid Re-hydration - Half fill all containers with corn. Empty into Instant Pot. Cover with water. Run 30 minutes on PC. Release, remove, dry.
  • Wheat/Rye Berry Soak Re-hydration - Half fill all containers with grain. Empty in pot with water. Soak for 6-12 hours, air dry until surface is completely dry.
  • Grain Jar Sterilization - Run 2 - 2.5 hrs on PC
  • PastyPlates, MEA Formulation- Run 45 minutes on PC
  • LME Liquid Culture - Run 45 minutes on PC


Edited by the_sonic16 (09/06/19 03:49 PM)


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Onlinebw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,457
Loc: 7b
Last seen: 3 minutes, 19 seconds
Trusted Cultivator
Re: Revitalize slow growing culture? [Re: the_sonic16]
    #26172645 - 09/06/19 03:53 PM (1 year, 5 months ago)

You should have saved your self a  plate/slant/grain master from generation one. Not much you can do now other than re plate or g2g and continue with the best growth.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineJohnRainy
Stranger

Registered: 07/09/19
Posts: 1,244
Last seen: 1 year, 1 month
Re: Revitalize slow growing culture? [Re: the_sonic16]
    #26172666 - 09/06/19 04:06 PM (1 year, 5 months ago)

Sounds like it's getting old and tired.  I think you are supposed to keep your source myc in the refrigerator.  Did you do that?

To keep it as vigorous as possible, Ive read that each agar transfer you do should have a different nutrient base.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineQuirkmeister92
Street Doctor


Registered: 02/01/18
Posts: 5,931
Loc: Grateful Dead USA
Last seen: 4 hours, 13 minutes
Re: Revitalize slow growing culture? [Re: JohnRainy]
    #26172674 - 09/06/19 04:09 PM (1 year, 5 months ago)

Is there any reason to use this culture to begin with or is it just what you have?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinethe_sonic16
Mushy McMusherson
I'm a teapot User Gallery


Registered: 03/10/19
Posts: 396
Loc: Somewhere off topic
Last seen: 30 days, 5 hours
Re: Revitalize slow growing culture? [Re: Quirkmeister92]
    #26172773 - 09/06/19 05:00 PM (1 year, 5 months ago)

I didn't store it in the fridge. I had been meaning to grow it out, but life got in the way. I just kept moving it to a new plate whenever it approached the edge of the plate instead.

I live in a state where it's tough to get spores and I enjoyed this varietal. Starting to look like I'm SoL at the rate it's currently growing. (I have some on grain and a plate).


--------------------
Sonic's "I'll buy a pressure cooker someday" Instant Pot times and settings:
  • Popping Corn Rapid Re-hydration - Half fill all containers with corn. Empty into Instant Pot. Cover with water. Run 30 minutes on PC. Release, remove, dry.
  • Wheat/Rye Berry Soak Re-hydration - Half fill all containers with grain. Empty in pot with water. Soak for 6-12 hours, air dry until surface is completely dry.
  • Grain Jar Sterilization - Run 2 - 2.5 hrs on PC
  • PastyPlates, MEA Formulation- Run 45 minutes on PC
  • LME Liquid Culture - Run 45 minutes on PC


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineJohnRainy
Stranger

Registered: 07/09/19
Posts: 1,244
Last seen: 1 year, 1 month
Re: Revitalize slow growing culture? [Re: the_sonic16]
    #26172838 - 09/06/19 05:38 PM (1 year, 5 months ago)

There are international vendors who will mail prints into the US states where spores are illegal.

As for your strain, yeah, it's probably on its way out.  The same thing happened to me.  I liked that strain too.  I still haven't found anything like it.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineQuirkmeister92
Street Doctor


Registered: 02/01/18
Posts: 5,931
Loc: Grateful Dead USA
Last seen: 4 hours, 13 minutes
Re: Revitalize slow growing culture? [Re: JohnRainy]
    #26173415 - 09/07/19 12:57 AM (1 year, 5 months ago)

It really shouldn't be senescence with just a half dozen transfers. I suppose you ought to grow it out and take some prints. You are growing spore factories really.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineJohnRainy
Stranger

Registered: 07/09/19
Posts: 1,244
Last seen: 1 year, 1 month
Re: Revitalize slow growing culture? [Re: Quirkmeister92]
    #26173577 - 09/07/19 05:51 AM (1 year, 5 months ago)

I think mine took about 7 or eight months, I had it incubated or at room temp the whole time, it was a  partial isolate from spores.

Wait a minute, now that I think about it, maybe I isolated it some during transfers and ended some symbiotic relationship between strains, reducing it's vigour, and the strain wasn't senescent at all!

Maybe thats what OP did.

How will we ever know?
              :willynilly:

Growing it out and taking prints is a splendid idea.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Edibles   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds Bulk Cannabis Seeds, High THC Strains   Left Coast Kratom Buy Kratom Capsules, Buy Kratom Extract, Kratom Powder For Sale   Amazon Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Slow growing mycelium J_Twista 2,027 4 06/16/01 06:49 PM
by Anno
* Slow Grow KatatoniK 1,218 6 09/14/01 05:12 AM
by NDK
* Slow growing mycelium FlyinBryan69 1,114 3 06/20/01 01:04 AM
by Zen Peddler
* slow growing mush loc567 873 2 07/28/02 12:29 PM
by Fd3000
* Agar and growing that white stuff! pj541 1,775 5 10/15/01 06:06 PM
by pj541
* VERY slow Orissas juubou 995 6 12/26/01 01:04 AM
by Richard_D_James
* Re: Growth In Super Slow-Mo Anonymous 1,051 7 12/28/99 08:25 PM
by Anonymous
* slow pins JameZTheNewbie 1,280 4 08/02/02 06:51 AM
by JameZTheNewbie

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
150 topic views. 21 members, 110 guests and 39 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Magic-Mushrooms-Shop.com
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.046 seconds spending 0.013 seconds on 16 queries.