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SHROOMSISAY01
Mr. Shrooms



Registered: 01/22/17
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Mushroom Generation Explanation Please!! 1
#26166868 - 09/03/19 01:07 PM (4 years, 4 months ago) |
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So my understanding is that a Generation only starts when you put a 1st generation mycelium onto the grain and if that grain was expanded that would be 2nd generation. As long as you are transferring 1st generation to Petri dishes it does not change the generation. Is this correct? Some explanation would be great here. Somehow I have gotten myself confused on the subject.
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WarFlo
Liquid Culture Adept


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Re: Mushroom Generation Explanation Please!! [Re: SHROOMSISAY01]
#26166924 - 09/03/19 01:44 PM (4 years, 4 months ago) |
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Also interesting, A lot has been explained in the books, but this moment... precisely - for some reason everyone bypassed.
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Quadman
Challenged


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Re: Mushroom Generation Explanation Please!! [Re: WarFlo]
#26167079 - 09/03/19 02:56 PM (4 years, 4 months ago) |
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Your master would be your original culture. Grow that out on grain you have your G1, expand that grain out with lets say grain to grain. Then those jars are G2 . G2 to grain your at G3. You get the idea.....
There is no real way to know the generation of your original culture. So you really cant account for that.
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SHROOMSISAY01
Mr. Shrooms



Registered: 01/22/17
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Re: Mushroom Generation Explanation Please!! [Re: Quadman]
#26167172 - 09/03/19 03:42 PM (4 years, 4 months ago) |
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Quote:
Quadman said: Your master would be your original culture. Grow that out on grain you have your G1, expand that grain out with lets say grain to grain. Then those jars are G2 . G2 to grain your at G3. You get the idea.....
There is no real way to know the generation of your original culture. So you really cant account for that.
Yea that is what I thought. But does transfers from Petri to Petri count as a generation? I don't think it does. Am I correct in saying That generation is only counted on grain and not on agar. This has been my long belief. But I have someone who disagrees with me.
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Quadman
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Re: Mushroom Generation Explanation Please!! [Re: SHROOMSISAY01]
#26167289 - 09/03/19 04:44 PM (4 years, 4 months ago) |
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I dont think technically it does, but if you were to transfer many times? Kinda of a grey area. Just like some of these cultures being swapped on the Shroomery, how many times have they been handled? Some have been treated like a whore.
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Edited by Quadman (09/03/19 04:45 PM)
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SHROOMSISAY01
Mr. Shrooms



Registered: 01/22/17
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Re: Mushroom Generation Explanation Please!! [Re: Quadman]
#26167338 - 09/03/19 05:19 PM (4 years, 4 months ago) |
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Quadman
Thanks for clearing that up. That is exactly how I thought it was. My friend and I were arguing about this and we decide to clear it up on here. Your statement made us both laugh. You brought back some fond memories of the good old days of trading whores.
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Inocybe
Stranger


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Loc: Spain
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Re: Mushroom Generation Explanation Please!! [Re: SHROOMSISAY01]
#26168441 - 09/04/19 09:38 AM (4 years, 4 months ago) |
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As far as i know, generation refers to grain spawn expansions, like Quadman said.
There is a labelling for petris though, Stamets exposes his method in his book, called "P value" which refers to how many times a culture was grown over a entire petri dish. P0 would mean that the culture haven't grown a full petri dish yet (for example a early isolate from spores or a clone from wild mushroom). If the culture grows out the petri dish, and it is transferred to a new dish, that dish would be P1, if transferred again then P2, etc. If you use an agar wedge from a petri to inoculate a grain jar or bag, that would be your grain master or master spawn (G1), that could be used for direct inoculation into substrate, or expanded to more grain bags/jars to get new generations from expansion (G2, G3, etc).
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