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OfflineGolem
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Theoretical Cotam Question
    #2616228 - 04/29/04 02:55 PM (12 years, 7 months ago)

I was wondering if there could be a contam that would not grow in a Karo TEK jar, but when injected into substrate (millet) would then start to grow.
And if there is such a contam, could it hurt to empty the syringe of liquid mycelium into some hydrogren peroxide to kill any spores of that contam before I knock up my millet.
I'm asking because I have some green growth on a wooden part of a window near my growing area that I just discovered. I bought a HEPA filter to clean out the room but I didn't have it when I did my Karo jar.
Thanks.


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InvisibleRipple
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Re: Theoretical Cotam Question [Re: Golem]
    #2616560 - 04/29/04 03:51 PM (12 years, 7 months ago)

Moving to proper forum.


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OfflineSuntzu
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Re: Theoretical Cotam Question [Re: Golem]
    #2618138 - 04/29/04 08:59 PM (12 years, 7 months ago)

My instinct is that a simple sugar medium [karo] will support anything millet will and more. You may be able to see it better on grain, though, because it's a 'dry' substrate where the colored conidia of the mold can develop better.

Peroxide isn't really a means to 'kill spores'. A peroxidated substrate makes spore germination difficult, so that to be effective the mycelium growth must occur in a peroxide environment. Simply adding it to a questionable syringe isn't going to be effective as using the syringe on a peroxidated substrate, because after the questionable syringe is added to the grain, it's no longer in a peroxidated environment. Doesn't sound very clear, does it make sense?

Basically, I'd give the karo a shot as is, and address furthur problems if they develop. Keep a non-innoculated negative grain control just in case.


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OfflineGolem
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Re: Theoretical Cotam Question [Re: Suntzu]
    #2619051 - 04/30/04 12:17 AM (12 years, 7 months ago)

Thanks. I'll do that.


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Mushrooms, Mycology and Psychedelics >> Contamination Forum

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