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Myyco.com Shop: Isolated Cubensis Liquid Culture For Sale

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Bluing on agar, or contam?
    #26154715 -










I did a set of agar-to-agar transfers a few weeks ago. I've been busy and haven't had time to do anything with them; over half of them show bluing to one extent or another, with a couple showing quite a bit of it. They've been in a somewhat hot room (~26-27C / 80F) during the day, so I don't know if they're stressed from heat and being overgrown, or there's a mold/contamination present.

I had to take the pics with curtains drawn as bright light tends to wash out the blue colour a bit and it doesn't show up as well, if that helps narrow it down.

Thanks for looking.

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Re: Bluing on agar, or contam? [Re: pkd]
    #26154762 -

I've seen that b4 in a dirty reishi plate. Blue/Green contam that gets covered with a super aggressive white myc that eventually turns a pinkish white. I would assume them to be fucked.


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Re: Bluing on agar, or contam? [Re: Dancing with Bears]
    #26154780 -

Dancing with Bears said:
I've seen that b4 in a dirty reishi plate. Blue/Green contam that gets covered with a super aggressive white myc that eventually turns a pinkish white. I would assume them to be fucked.


Hmm, these plates are roughly 4 weeks old now. Definitely no pinkish colour thus far.

Just went back and checked all the donor plates these transfers were taken from. All of them showing the same blue. I took the transfers from those plates about 12 days after inoculation by MSS. They definitely weren't showing any blue at the time I took the transfers.

Not sure what to make of it.

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Re: Bluing on agar, or contam? [Re: pkd]
    #26154789 -







A few of these plates taken in better light.

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Re: Bluing on agar, or contam? [Re: pkd]
    #26161865 -

Anyone?

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Re: Bluing on agar, or contam? [Re: pkd]
    #26161889 -

Idk the middle plate is obviously pinning. Transfer a pin to a clean plate and start fresh

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Re: Bluing on agar, or contam? [Re: pkd]
    #26161963 -

Unfortunately no answer/ID for you but it isn't very confidence inspiring and I personally wouldn't feel comfortable expanding it as-is.
I haven't seen bruising on agar before + where its showing up(which dishes) leads me to believe its a contam thats coming with the transfers and I'd attempt to isolate away from it(or start with a different culture if you have the option). I get the thought that the temp difference would also make sense as far as affecting all the plates but I'm pretty sure my a/c drip line has backed up-shutting my a/c off long enough the room to reach 80 or higher and haven't noticed any bruising from it so I'm reluctant to blame that

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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