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Re: Verum's Liquid Culture tests [Re: J. Jack Flash]
    #26165394 -

Anything can be done in a sab if you've got your tek right. It's just more of a pain in the ass. I only bust out my sab if i'm trying to clean up a sporalating plate, take a tissue transfer or make a print.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis]
    #26165406 -

And just a random thought.  Soft agar method might be better, safer, faster,  than either method.  But I’m going to do an LC or two regardless


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Wide Mouth 1/2 Pint No-Pour TEK
TC Teks & Links

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Re: Verum's Liquid Culture tests [Re: Just_A_Noob]
    #26165447 -

Soft agar blended.... LI? or just to grain and smashed around?


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis]
    #26165466 -

Just to grain and smashed around


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Wide Mouth 1/2 Pint No-Pour TEK
TC Teks & Links

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Re: Verum's Liquid Culture tests [Re: Just_A_Noob]
    #26165508 -

V.- I think someone taking the time to run an actual test is great and ppl will learn a lot.

I've told you I don't hate on LC. I think it's an awesome and powerful tool. I've used it for years.

Here's my take on the 2 methods so far. Both kick ass and can yield fuck tons of spawn. And your sterile tek should be on point with both. Each are just a different method of expansion. I don't really think one is much more work than the other.

But I think ppl would have a better appreciation for a2g and g2g if they used the proper ratios and shook stuff when it needs to be without waiting too long.

Both are beautiful means to an awesome end result.

:dj:


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Verum's Liquid Culture tests [Re: LotKid]
    #26165558 -

For fuckin sure. Well said my friend!

I'm not exactly sure how to do an LC vs G2G test as far as ratios of innoculant to make it "fair". There are so many variables it would be hard to conclusively determine which is "easier" and "faster"


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis]
    #26166782 -

When I was using LC I'd inoculate with 1-2 cc's.

Just spitballing here but what about something like... 10-20cc of LC equals a master jar? 20 cc's 0f LC spread across 10 bags and 1 master jar spread across 10 bags.

I think you should see similiar speeds between the 2.


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:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
:dancingbear: :dancingbear:

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Re: Verum's Liquid Culture tests [Re: LotKid]
    #26166838 -

I think G2G would win in that scenario. But I'll admit, I never really rush things. For instance, I have twenty or so bags chillin in the room that could easily be done by now if i had only shaken them last week. My point is, I don't know how quickly i could get a few cc's to consume a bag.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis]
    #26167135 -

updated op with pics of plates and a little description of what i think.

Edit 9/3/19

It's been about two days (i think) and i feel comfortable declaring these plates, and subsequently these LCs, clean.
Here are the LCs pre swirl.
At this point they are just over 2 weeks old.



Post swirl
(shown in same order as above)
1000cc 0.4%, 500cc 0.4%, 500cc 2%, 1000cc 2%

     

The plates, two pics of each, in the same order.

1000cc 0.4%
 

500cc 0.4%
 

500cc 2%
 

1000cc 2%
 

The pics must be expanded and studied closely to see whats going on. Even in person i have to look closely to be sure of what i'm seeing. The two things i like to really look at are the obvious growth spots and the streak pattern. Any time you can see good portions of the streak not showing any growth, it's a good thing. This because the myc doesn't spread throughout the entirety of the liquid the way that bacteria or yeast does. If these LCs had bacterial infections you'd be able to see the slimy snotty bacterial growth all along the streak pattern.

You'll notice heavily concentrated growth, that doesn't really stick with the streak pattern, at the beginning of each streak. This is because the drip is pulled off the loop and forms a small puddle when the loop hits the agar. This distributes tiny myc nuggets all over the area. You then pull the loop through it and grab whatever you grab. Then, as you streak the plate, little nuggets fall off here and there becoming less and less concentrated as you streak.

Another thing to notice, on the "2A" plate, there are two noticeably bigger growth spots. These might be confused as being a problem by the untrained eye. The fact that they are so fast (compared to areas around them) and that they seem puffy and disorganized might cause some concern. But not I. It is my opinion that these are the result of slightly larger mycelial nuggets that more or less land in a pile... like a spaghetti noodle that's been folded over and over on itself. This growth on top of growth on top of growth can cause some abnormal appearances but will clear itself up in a few days.

It's appropriate to note that this is precisely why I prefer to leave my LCs on a stir plate. Not only does it exponentially accelerate growth, it also keeps larger myc nuggets from forming and that's how i like my LCs simply packed full of tiny tiny tiny magical mycelial fragments. It's like G2Ging with grass seed or wbs compared to oats. Simply more inoculation points per cc.

I'm going to go make up some WBS. I'll upload a few more pics in a day or two to show the growth on the plates.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis]
    #26167155 -

:jackiechanofapproval:

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Re: Verum's Liquid Culture tests [Re: Failboat]
    #26174691 -

updated with


edit 9\7\19

Almighty mush cult fam, the show must go on! I was busy for a couple days but got around to noccing up a bag or two with these here liquids. I'm a little bummed though because I took the time to tape my camera to the ceiling and get it dangling at just the right angle to give ya'll a good view. Set the fucker to video the session and then got to work, being sure to give ya'll a nice good look at each syringe and how i position the bags and..... all that jazz. The video didn't make it beyond me getting set up to roll. The low battery warning came on and apparently ends the video when it does. What a stupid design.

Anywho. I did a 10cc, a 20cc. and a 35cc bag with the 0.4% and the same with the 2%. I figure that'll be a fun show. Then, mid session, I decided to free pour a bag or two to get a better understanding of free pouring vs shooting from a syringe. It's definitely nice to be able to spray it all over with a syringe. The free pour doesn't get spread around as nicely. I'll update when some growth becomes obvious.



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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis]
    #26174717 -

lol man i hate when shit like that happens. its ok tho you got enough cred on here we all take ur word and can imagine it would have been great to watch 🤘


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PANAEOLUS FRUITING MADE SIMPLE


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Re: Verum's Liquid Culture tests [Re: jcm4620]
    #26190382 -

updated op. bags are looking good.


Edit 9\15\09
I let these sit a bit longer than needed before shaking just to give a little better understanding of where the myc took off on each bag. Here they are today. Every side of every bag. Except the 10cc from the 2%. I accidentally forgot to photograph it before mixing.

0.4% 35cc


     

0.4% 20cc

     

0.4% 10cc

     

2% 35cc

   

2% 20cc

     

2% free pour 20ish cc
On this one i tried to make a nice streak across the middle of the bag but it's a little hard to aim. I only took pics of the sides showing growth on these guys.

   

This one got two streaks 30 - 40ish cc 

   

I often hear people asking if it's ok to leave standing LC at the bottom of jars or bags. You'll notice that most of these (specially the free pour and 35cc bags) have a ton of colonized grain on the bottom. This was standing/ pooled LC. I like to prepare my grain dry enough to handle lots of LC. I've run into no problems with standing LC as long as the grain isn't too wet.

All bags were shaken well and placed back on the shelf.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis] * 1
    #26190460 -

:popcorn:  :camping:


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C10’s agar guide
Good surface conditions = Good pinsets
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Re: Verum's Liquid Culture tests [Re: AyePlus]
    #26190568 -

:rockon: love the pictures verum!


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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

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Re: Verum's Liquid Culture tests [Re: eatyualive] * 1
    #26190732 -

Thanks man. I've said this in some other threads (probably this one actually but), when i was a noob I read through every thread i could find over and over and really really appreciated the ones that had a little time, thought and energy put into them. So now it's my time to give a little back. I try to make them as helpful as possible for folks who are trying to get it figured now.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis]
    #26192083 -

:threadmonitor:

I have the same temp gun :wink:


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My Shoebox Factory Beginner's Tek | Shoebox Assembly Tek | Methodology/teks I use | How I Store Bulk Items

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Re: Verum's Liquid Culture tests [Re: ShaperDreaming]
    #26192092 -

It comes in rather handy


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis]
    #26201827 -

updated with

Edit 9/21/19

Here they are folks
0.4% left to right; 35cc, 20cc, 10cc



2% in the same order



Free pour
The left got one quick streak and the right got two


It's looking like the growth of the 0.4% and 2% are pretty much the same. And, as one would assume, the bags that got more are farther along. I don't think it'll make a huge difference in the end though, maybe a couple days. I haven't decided whether or not to shake them again. The ones that got less would definitely benefit from a shake but that'd kind of throw off the experiment so.... Thoughts? I'll leave it up to you.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: Verum's Liquid Culture tests [Re: verum subsequentis]
    #26201836 -

verum subsequentis said:
.... Thoughts? I'll leave it up to you.


id leave em and watch:popcorn:

yay for .4%
:thatsayes:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Shop: Mushroom-Hut Liquid Cultures   Sporeworks.EU Spores for European Microscopy   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Autoflowering Cannabis Seeds   North Spore Injection Grain Bag

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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