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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
'wood lovers agar'
    #2615281 - 04/29/04 10:44 AM (13 years, 1 day ago)

Rush Wayne suggests adding 'wood pellets' (which presumably break down during sterilisation) to to a MYD agar mix...

I can't get the pellets here, but I do have some suitable rough sawdust I plan to use as substrate...  I was wondering if I could perhaps make a 'sawdust tea' (boil a cup or so for 1/2 an hour and save the liquid) as the liquid base for a wood lovers agar mix.

Also, I can't get malt (or yeast extract :frown: ), so I was planning on trying potato/dextrose (read table sugar) with some of my 'sawdust soup'...

Any suggestions?  (please keep in mind that I live in a country where I can't read the labels on anything to see if there are additives or not... :wink: )


--------------------
Shrooms aren't everyone's cup of tea... (Some folks just eat 'em)


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Offlinezeronio
Stranger
Male

Registered: 10/16/01
Posts: 2,349
Loc: Slovenia
Last seen: 7 months, 17 days
Re: 'wood lovers agar' [Re: sakura]
    #2615356 - 04/29/04 11:15 AM (13 years, 1 day ago)

You don't have to make a tea from sawdust - just put few grams of sawdust in the agar. That should work.
Dextrose is not the same as table sugar. It's good to add some simple sugars like dextrose, fructose or maltose in the agar. If you can't find anything like that use honey!


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OfflinecharlieXxXxX
Stranger

Registered: 03/29/04
Posts: 80
Loc: Earth, USA, WI, Madison
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Re: 'wood lovers agar' [Re: sakura]
    #2615970 - 04/29/04 01:36 PM (13 years, 1 day ago)


Yup the wood pellets are jus compressed sawdust.
When you pour hot water on them, they dissolve.

DFA - 1 litre water
20g agar
20g dry dog food


I would add the wood also.


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Invisiblebrainbreath
perpetual newbie
Registered: 06/13/03
Posts: 67
Loc: down on the corner
Re: 'wood lovers agar' [Re: charlieXxXxX]
    #2616180 - 04/29/04 02:41 PM (13 years, 1 day ago)

karo syrup is dextrose. I usually add a couple grams of whatever I'm going to grow on to my agar, wbs, sawdust, straw etc. The myc is then prepared for the substrate and colonization is sped up quite a bit.


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OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 9 years, 3 months
Re: 'wood lovers agar' [Re: brainbreath]
    #2616903 - 04/29/04 04:39 PM (13 years, 1 day ago)

karo syrup is not dextrose. I believe it contains more fructose than dextrose, yes it is interchangeable, but no it is not dextrose. (pure)


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: 'wood lovers agar' [Re: sakura]
    #2617140 - 04/29/04 05:24 PM (13 years, 1 day ago)

Sucrose (table sugar) works fine as well. I've tried it with numerous species and had no problems.


--------------------
PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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OfflineMycena
mycoexplorer
Registered: 05/02/03
Posts: 270
Loc: indonesia
Last seen: 5 years, 11 days
Re: 'wood lovers agar' [Re: sakura]
    #2655846 - 05/09/04 06:02 AM (12 years, 11 months ago)

The wood tea agar works very well at sorting what species will like your wood

Ive been testing woodlovers on eucalyptus sawdust and seen vast differences between the species and between isolates

For example the Pleurotus autralis (native) did very well, as did some shiitake strains, while others lagged. The lions mane didnt respond very well at all.
This corresponds to the effects ive been getting when trialling these species on supplemented Eucalptus sawdust blocks

I also had zero contamination on the Tea agar. The Eucalyptus phenols and oils seems to inhibit all the moulds and bacteria, of course it also inhibited many edible fungi
Im using a Eucalyptus species milled locally (there are hundreds of species) that they annoyingly just call 'hardwood'. But ive seen the forests it comes from and this is a very widespread species so i should be able to get the species name easily

anyway point to that is that not all eucalyps are equal. E globulus and E saligna (bluegums) work much better than Rivergum (E camaldulensis) . Stringybarks are also good (E marginata)for shiitake.
To find out all this would take years and years and lots of $ to get large volumes of sawdust shipped in from all over. Using Tea agar we can screen potential species much faster and more cheaply.


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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