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Offlinenubgrower
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Re: BOD's DIY Inoculation Loop TEK [Re: nubgrower]
    #24782259 - 11/14/17 10:53 AM (6 years, 3 months ago)

Bod, I assume that you just lightly slide the loop across the surface when you streak?  Not putting any real pressure, not leaving a dent or a cut.....

just making sure.
cheers


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Great teks here.
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InvisiblebodhisattaMDiscordReddit
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Re: BOD's DIY Inoculation Loop TEK [Re: nubgrower]
    #24782304 - 11/14/17 11:20 AM (6 years, 3 months ago)

Yea if it scrapes a bit oh well tho


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OfflineFriendOfTheDevil70
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
    #24957353 - 01/31/18 03:34 PM (6 years, 16 days ago)

I've seen your video using the loop with the print, but are you using a syringe for the initial drop directly onto plate then Z smear with loop? Or where are you getting solution from?

Thanks so much.

EDIT: I saw the answer in your agar tek. Sorry for inconvenience.


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Set out runnin' but I take my time
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If I get home before daylight, I just might get some sleep tonight



Edited by FriendOfTheDevil70 (01/31/18 03:36 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's DIY Inoculation Loop TEK [Re: FriendOfTheDevil70] * 1
    #24957370 - 01/31/18 03:42 PM (6 years, 16 days ago)

Squirting onto the loop. Or squirt spore solution into a sterilized cup like a shot glass then dip in that


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Offlinedreamwever
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
    #25348409 - 07/26/18 03:49 PM (5 years, 6 months ago)

Thanks Bod for the DIY inoculation loop. It seems a better way to get the spores up from the foil.  Any spores left will use the 1mm shringe with droplets of distilled water and experiment with remaning spores

Heat the loop and cool in distilled water? is that okay to use

Also, have 20awg audio tinned wire? could this be used? it relatively sturdy, otherwise i can get approxiate wire here in Australia

Few cheep loops on ebay, but shipping ups the price


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OfflineManifoldPrime
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Re: BOD's DIY Inoculation Loop TEK [Re: dreamwever]
    #25349673 - 07/27/18 03:33 AM (5 years, 6 months ago)



Bout time I upgraded my loop. Currently using 2 paper clips bent into a loop and wound on a pencil, taped, and PC'd. Hella ghetto but it works


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's DIY Inoculation Loop TEK [Re: ManifoldPrime] * 1
    #25349725 - 07/27/18 05:00 AM (5 years, 6 months ago)

Yea that's a bit ugly haha


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Offlinedreamwever
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta] * 1
    #25365424 - 08/03/18 10:11 PM (5 years, 6 months ago)

my 2 DIY Innoc loops 28awg nichrome wire



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OfflineRowboat
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
    #25478954 - 09/21/18 07:18 PM (5 years, 4 months ago)

Quote:

Bod's DIY Inoculation Loop TEK
Using X-Acto style handles and Nicrhome wire or easily found as vape coil wire





This being apart of such a great source of information, I figured I'd mention Nichrome is misspelled here in the title if it matters.


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's DIY Inoculation Loop TEK [Re: Rowboat]
    #25479151 - 09/21/18 08:22 PM (5 years, 4 months ago)

Good catch haha


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Invisiblettching8475
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
    #25479304 - 09/21/18 09:33 PM (5 years, 4 months ago)

Well timed bump of this thread.


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OfflineSeverance13
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Re: BOD's DIY Inoculation Loop TEK [Re: ttching8475]
    #26009310 - 05/23/19 12:42 PM (4 years, 8 months ago)

Alright everyone. I know Bod suggests using whatchu got for simplicity, but I did some research, and it turns out 25 or 26 g kanthal or nichrome 80 would be optimal. The thinness guarantees better cooldown times. I noticed my current one made with 20g kanthal sizzles my agar during cooldown for nearly 30 sec. As we all know, exposure time matters in this hobby.

I also posted a link earlier about another tool, a Fungus Hoe, that seems to be perfect for ripping and transferring mycelium to new medium. Here's an updated link. What do y'all think?


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Invisiblewildernessjunkie
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Re: BOD's DIY Inoculation Loop TEK [Re: Severance13]
    #26010376 - 05/24/19 12:50 AM (4 years, 8 months ago)

I used stainless steel picture hanging wire from the hardware store for my first loop. Works great, and I still use it.

The pics in your that link you posted are broken BTW.


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InvisibleSir Pentinite
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Re: BOD's DIY Inoculation Loop TEK [Re: wildernessjunkie]
    #26011394 - 05/24/19 04:44 PM (4 years, 8 months ago)

Since I've been dragging my feet on making an introduction thread, maybe my first post can be a contribution. :cookiemonster:

One way to scavenge Nichrome wire is from wirewound electrical resistors. They are typically found as the rectangular ceramic type meant to handle a lot of current and heat. I broke open a 10 ohm/10 watt power resistor and it had about 14" of 32awg Nichrome. Lower ohms with higher wattage ratings would probably have thicker wire.

I wouldn't suggest buying resistors just for the wire, but if you already have some...


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"I thought to myself 'Boy, I'm sure glad there's nobody here to see this because this is exactly the sort of thing that gets people riled-up and they assume you're dying and that something has to be done. Where if you're alone, you know, you either come through it or you die, but in any case you avoid the fuss.'"
- Terrence McKenna


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OfflineDanito
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta]
    #26133990 - 08/13/19 03:34 PM (4 years, 6 months ago)

Great stuff Bod thank you. I spent an hour earlier inoculating with one of those little "1 microliter" loops you mentioned, and afterwards started to worry that it might have been too small for the job. I bought them in complete noob ignorance from Ebay.

Three quick questions please:

1. Being plastic and not able to be flamed, do I need to use a different loop with each petri?

2. Can I PC the used loops to re-sterilise them?

3. Is there any advantage to using the pre-sterilised loops over following your DIY loop tek?


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"Our culture is not our friend" Yuval Noah Harari

Change your self, don't try to change the world


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's DIY Inoculation Loop TEK [Re: Danito]
    #26134047 - 08/13/19 04:02 PM (4 years, 6 months ago)

1. Yes
2. Probably not
3. If you like the disposables


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Offlinevinnie boombotz
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Re: BOD's DIY Inoculation Loop TEK [Re: bodhisatta] * 1
    #26206481 - 09/24/19 12:38 PM (4 years, 4 months ago)



Incredible. This is a must, anyone who works with agar needs to make these


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Offlinemi.ro.ku
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Re: BOD's DIY Inoculation Loop TEK [Re: Organic_Magic]
    #26216266 - 09/28/19 02:11 PM (4 years, 4 months ago)

Good tek :wink:


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OfflineDeadlySin
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Registered: 09/25/19
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Re: BOD's DIY Inoculation Loop TEK [Re: mi.ro.ku]
    #26247135 - 10/12/19 11:02 AM (4 years, 4 months ago)



Great Tek. Here is my attempt.


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InvisiblebodhisattaMDiscordReddit
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Re: BOD's DIY Inoculation Loop TEK [Re: DeadlySin]
    #26247201 - 10/12/19 11:40 AM (4 years, 4 months ago)

Looks like more of a success than an attempt


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