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Offlinefallenmtn
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steer manure substrate fail
    #26133854 - 08/13/19 04:02 PM (1 year, 6 months ago)

Two Monotub setups with manure substrate.

It's been 12 days and it doesn't seem like the mycelium is spreading--just growing off the spawn.  The substrate is damp, temp around 73 degrees.  I open the lids every night.

For prep, I baked the manure in the oven to sterilize.

Any ideas why I'm failing?

Tx ahead of time.


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OfflineMorel Guy
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Re: steer manure substrate fail [Re: fallenmtn]
    #26133858 - 08/13/19 04:04 PM (1 year, 6 months ago)

I'm guessing baking poop in the oven is not a good sign of being smart.


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"in sterquiliniis invenitur in stercore invenitur"

In filth it will be found in dung it will be found


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Offlinefallenmtn
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Re: steer manure substrate fail [Re: Morel Guy]
    #26133890 - 08/13/19 04:24 PM (1 year, 6 months ago)

Super helpful.  :undecided:


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OfflineCactiChris
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Re: steer manure substrate fail [Re: fallenmtn]
    #26133899 - 08/13/19 04:32 PM (1 year, 6 months ago)

I am definitely not a pro at this but The last time  I used manure i found it took a bit longer then say just coir.I want to say it is because if the PH levels or possible acidity. Not 100% sure.  Give it a little time see what nature dose.


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Offlinefallenmtn
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Re: steer manure substrate fail [Re: CactiChris]
    #26133904 - 08/13/19 04:34 PM (1 year, 6 months ago)

Thanks, CactiChris.  I didn't think about pH and I can test it tonight.


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InvisibleJHOVA
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Re: steer manure substrate fail [Re: fallenmtn]
    #26133928 - 08/13/19 04:52 PM (1 year, 6 months ago)

Pasteurize in jars on your stove. Bring the jars up to 100 f Internally then let the heat rise. Start your timer when it hits 140 and wait an hour. Don't go over 160. Use a meat thermometer or digital wireless thermometer .


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OfflineDnDRnD
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Re: steer manure substrate fail [Re: fallenmtn]
    #26133930 - 08/13/19 04:54 PM (1 year, 6 months ago)

So I havnt ran a mono but ive done a couple manure substrates before in smaller sized containers

- manure is supposed to be pasteurized NOT sterilized, when its pasturized it's more resistant to contamination because it still has some beneficial microbes to protect it.

- manure shouldn't be cooked in the oven, check out franks stove top pasteurization Tek

- is it just straight manure? What's the substrate mixed of?

- was the spawn clean? It could not be colonizing because of bacteria


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662



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OfflineShaperDreaming
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Re: steer manure substrate fail [Re: DnDRnD]
    #26133986 - 08/13/19 05:32 PM (1 year, 6 months ago)

Quote:

DnDRnD said:
- manure is supposed to be pasteurized NOT sterilized, when its pasturized it's more resistant to contamination because it still has some beneficial microbes to protect it.



Just want to chime in that you can sterilize it, but then you need to work aseptically until it is fully colonized (similar to how you do for your grains). It is also a huge pain in the ass to do and easily contaminates.


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OfflineDnDRnD
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Re: steer manure substrate fail [Re: ShaperDreaming]
    #26134022 - 08/13/19 05:50 PM (1 year, 6 months ago)

Quote:

ShaperDreaming said:
Quote:

DnDRnD said:
- manure is supposed to be pasteurized NOT sterilized, when its pasturized it's more resistant to contamination because it still has some beneficial microbes to protect it.



Just want to chime in that you can sterilize it, but then you need to work aseptically until it is fully colonized (similar to how you do for your grains). It is also a huge pain in the ass to do and easily contaminates.




Oh true true! Ala macmerdins pan cakes and manure brf


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662



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Offlinefallenmtn
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Re: steer manure substrate fail [Re: DnDRnD]
    #26134271 - 08/13/19 08:13 PM (1 year, 6 months ago)

I'm using bagged manure that has been composted.  Looks like it has shredded wood and other stuff because it is fluffy even when wet.

I did wipe the tubs down with alcohol before use.  I don't see anything else growing.

I'll check oh and take pics tonight.


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OfflineDnDRnD
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Re: steer manure substrate fail [Re: fallenmtn]
    #26134276 - 08/13/19 08:19 PM (1 year, 6 months ago)

You cant normally see bacteria (you can see signs of it though)

If it's got wood chips in it you'd want to take them out


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662



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OfflineJohnRainy
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Re: steer manure substrate fail [Re: fallenmtn]
    #26134286 - 08/13/19 08:24 PM (1 year, 6 months ago)

Are we talking about 100% manure here?  It's too dense for myc to penetrate, it cannot really run through it.

Ive used manure before but mixed in with lots of chopped straw and verm and gypsum. 

You didn't use 100% manure did you?


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Offlinefallenmtn
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Re: steer manure substrate fail [Re: JohnRainy]
    #26135174 - 08/14/19 11:39 AM (1 year, 6 months ago)

I attempted to post last night but it didn't take.

I tried two different pH meters and both measured at 7.  I was really hoping pH was the issue but I guess not.





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OfflineFun-G
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Re: steer manure substrate fail [Re: fallenmtn]
    #26135259 - 08/14/19 12:21 PM (1 year, 6 months ago)

Your manure looks a wee bit on the wet side.

When I used horse manure I'd just hot water bath it in a cooler inside of a laundry bag.... there is a tek for it. Then after the pasteurization just squeeze it until only a few droplets of water came out.


I'm going to go with it being a moisture issue.


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OfflineDnDRnD
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Re: steer manure substrate fail [Re: Fun-G]
    #26135338 - 08/14/19 01:03 PM (1 year, 6 months ago)

Quote:

Fun-G said:
Your manure looks a wee bit on the wet side.

When I used horse manure I'd just hot water bath it in a cooler inside of a laundry bag.... there is a tek for it. Then after the pasteurization just squeeze it until only a few droplets of water came out.


I'm going to go with it being a moisture issue.




Isnt that the bucket tek for coco coir minus the pillow case? I've never heard of pasteurizing manure that way


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662



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OfflineAtmozFear
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Re: steer manure substrate fail [Re: Fun-G]
    #26135361 - 08/14/19 01:15 PM (1 year, 6 months ago)

Took me a long time to realize how much water is in any substrate after properly prepping, even when it appears dry.


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Offlinefallenmtn
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Re: steer manure substrate fail [Re: AtmozFear]
    #26135370 - 08/14/19 01:17 PM (1 year, 6 months ago)

Thanks!  I'm starting another tub this weekend.  I'll go for dryer.


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OfflineDnDRnD
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Re: steer manure substrate fail [Re: fallenmtn]
    #26135393 - 08/14/19 01:33 PM (1 year, 6 months ago)

Also is that substrate straight manure?


--------------------
Bods Easy AF Oat Prep Tek
https://www.shroomery.org/forums/showflat.php/Number/24126032

Pastywhytes No Pour Agar Tek (PastyPlates)
https://www.shroomery.org/forums/showflat.php/Number/19208976

Shaperdreamings Shoebox Assembly Tek
https://www.shroomery.org/forums/showflat.php/Number/26009662



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Offlinefallenmtn
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Re: steer manure substrate fail [Re: DnDRnD]
    #26137039 - 08/15/19 01:51 PM (1 year, 6 months ago)

I think I'm going to find some plain kitty litter (bentonite) and spread a little on top to absorb some of the moisture.


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OfflineShaperDreaming
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Re: steer manure substrate fail [Re: fallenmtn]
    #26137115 - 08/15/19 03:05 PM (1 year, 6 months ago)

I really hope your joking :facepalm3:

That's going to make it hellish for your myc to grow in, and likely won't do what you want it too. If you really need to you can use some paper towels to blot it out, but just leave the lid off for 8-24 hours and you should end up with a dry enough substrate.


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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