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OfflineABShaman
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Agar first timer contaminants assessment
    #26130689 - 08/11/19 08:27 AM (1 year, 6 months ago)

Hi,

So when I first got on here I was told that to verify my liquid culture I should put it into some agar and find out the results.

Here it is with pics I set the ones that look ok to the side amongst the obviously ones.

5 decent looking plates
3 Strains, order decending.
top 2 are really old culture - (Dream Weaver)
and middle 2 are new culture (Unknown1)
bottom is one is a single (Unknown2)














My questions are
1. Which ones are good
2. Are they done colonizing or should I give them more time?
3. Are ones that have contams in some places but not others salvageable ?

Thank you 🙏🏼


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InvisiblebodhisattaM
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Re: Agar first timer contaminants assessment [Re: ABShaman]
    #26130745 - 08/11/19 09:28 AM (1 year, 6 months ago)

Looks like you just shot LI right onto a plate so it's hard to tell anything. You should streak the LI inoculant across the agar with an inoculation loop so you can see if any contaminants grow long before the plate is over colonized


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Re: Agar first timer contaminants assessment [Re: bodhisatta]
    #26130753 - 08/11/19 09:33 AM (1 year, 6 months ago)




Cant tell much from your plates


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Re: Agar first timer contaminants assessment [Re: ABShaman]
    #26130758 - 08/11/19 09:34 AM (1 year, 6 months ago)

Part of the goal of agar is to get some clean growth. You would then inoculate a new plate with a wedge from the clean section. Repeat this process until you have a contamination-free plate, then use wedges from that clean plate to inoculate grain or LC or whatever else you're hoping to do with it.

In my opinion, it isn't entirely necessary to wait for agar to completely colonize. As I said before, we're just trying to get some clean growth. For example, I had some very stubborn spores that I couldn't get to germinate on agar, so I threw them in a liquid culture and hoped for the best. Once that LC got going I used it to inoculate agar. Within a couple days I had some good mycelium growing on the agar, but I assumed I also had contaminates. I took a wedge and transferred it to a new plate which grew well and did not contaminate. A wedge from that plate was used to inoculate liquid culture.

You can read for days on end and never feel like you know enough. Sometimes doing is the best way to learn. Get going and make some mistakes!


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OfflineABShaman
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Re: Agar first timer contaminants assessment [Re: bodhisatta]
    #26130796 - 08/11/19 10:15 AM (1 year, 6 months ago)

Thats true 😆. I thought the other method was only for spores and you can just squirt some LC in there. Was I suppose to inject it?


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