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OfflinePuduwoke
mushroom enthusiastic

Registered: 06/25/17
Posts: 1,666
Last seen: 16 hours, 41 minutes
Re: Can you come from cracker dry tissue? Let's find out [Re: k5hd2y]
    #26123799 - 08/06/19 08:22 AM (4 years, 6 months ago)

Quote:

El Chupacabra said:
:threadmonitor:



Quote:

k5hd2y said:
So glad to be back!
I see verums been working:pipesmoke:




:whathesaid:


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Offline1WithU
Inspired Consciousness
I'm a teapot User Gallery


Registered: 11/10/15
Posts: 404
Last seen: 8 months, 2 days
Re: Can you come from cracker dry tissue? Let's find out [Re: Puduwoke]
    #26125397 - 08/07/19 09:54 AM (4 years, 6 months ago)

very curious to see an update on this project.  Crossing fingers for ya!  :goodluck:


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Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
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OfflineEl Chupacabra
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Registered: 01/07/19
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Re: Can you come from cracker dry tissue? Let's find out [Re: 1WithU]
    #26125529 - 08/07/19 11:47 AM (4 years, 6 months ago)

Quote:

Munchauzen said:
I've personally tried reviving dehydrated fruits with no success, but naturally desiccated fruits I've had success on more than one occasion. I've even transferred an entire desiccated plate once, and it took off just fine.




Mine failed.
From cracker dry dehydrated fruits all I grew was all kinds of bacteria and so I tried hot pour on the bacteria laden plates and ended up with a little bit of what I think was trich. Never saw anything that looked like mushroom myc on any of my plates.
I'm anxious to see if Verum has had any luck.

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Offlineverum subsequentis
seeker of truth
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Registered: 03/22/16
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Re: Can you come from cracker dry tissue? Let's find out [Re: El Chupacabra]
    #26125563 - 08/07/19 12:29 PM (4 years, 6 months ago)

I did manage to get some growth. I'll snap some pics and upload in a minute. Anyone know if the site is up and running on the pics department?

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OfflineMLPismyOPSEC
That One Ponyfucker
I'm a teapot

Registered: 11/13/18
Posts: 884
Loc: Equestria? Mordor? Wester... Flag
Last seen: 23 days, 13 hours
Re: Can you come from cracker dry tissue? Let's find out [Re: verum subsequentis]
    #26125586 - 08/07/19 12:41 PM (4 years, 6 months ago)

Not yet, Ythan stated within the week they should be working. They are transferring all the image data, nothing was lost, it'll just take some time. You can still upload for now, but only the thumbnail will show.

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Offlineverum subsequentis
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Re: Can you come from cracker dry tissue? Let's find out [Re: MLPismyOPSEC]
    #26125610 - 08/07/19 01:00 PM (4 years, 6 months ago)

Word

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Offline1WithU
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Re: Can you come from cracker dry tissue? Let's find out [Re: verum subsequentis]
    #26125661 - 08/07/19 01:36 PM (4 years, 6 months ago)

Quote:

verum subsequentis said:
I did manage to get some growth. I'll snap some pics and upload in a minute. Anyone know if the site is up and running on the pics department?




Soooo... does this mean that it worked???

On a side note, I heard that if you take a stone (sclerotia) that is dried fully, rehydrate it in h2o2 water, that it'll grow too.  That surprised me.  Now from cracker dry fruit tissue?  Wow!

Back to the main question... so are you saying that this worked for you?

And, how old was the cracker dried fruit you started with?


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Explore the transformative benefits of microdosing.
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OfflineLtLurker
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Re: Can you come from cracker dry tissue? Let's find out [Re: 1WithU]
    #26125723 - 08/07/19 02:11 PM (4 years, 6 months ago)

:popcorn:

@1withu - Could be true. h2o2 will quickly break down into water, so maybe your killing a little surface stuff than just re-hydrating it. Probably helps that stones are the energy reserve for tough conditions already.

Real question is if you can get piece to grow and clean it enough to be usable. Solid stone sounds easier to get a clean center sample from than a hollow cube that's probably had some air up in there.

Edited by LtLurker (08/07/19 02:18 PM)

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Offlineverum subsequentis
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Re: Can you come from cracker dry tissue? Let's find out [Re: LtLurker] * 1
    #26125737 - 08/07/19 02:25 PM (4 years, 6 months ago)

Updated op.

No, I wasn't saying that i worked. I was simply stating that i got growth. Sorry for being an ass, i was just toying with ya. So far nothing but mold and bacteria.

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Offline1WithU
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Re: Can you come from cracker dry tissue? Let's find out [Re: verum subsequentis]
    #26126889 - 08/08/19 12:30 PM (4 years, 6 months ago)

Quote:

verum subsequentis said:
Updated op.

No, I wasn't saying that i worked. I was simply stating that i got growth. Sorry for being an ass, i was just toying with ya. So far nothing but mold and bacteria.




Well thanks for trying the experiment ya did.  Now we all know... doesn't work.  Thank you for that.

I guess one take away from this thread is that if you happen to get dried shrooms from somewhere that you liked, try some gill tissue for the spores there, but the dried fruit, other than the spores in the gills, is pretty much dead.


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Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"


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Offlineverum subsequentis
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Re: Can you come from cracker dry tissue? Let's find out [Re: 1WithU]
    #26126903 - 08/08/19 12:46 PM (4 years, 6 months ago)

Not so fast friend. One of the biggest problems in mush cult is coming to conclusions too quickly. That's not how science works. I'll have to try this again and again before i make an official statement. It would appear that it doesn't work though.

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Offline1WithU
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Re: Can you come from cracker dry tissue? Let's find out [Re: verum subsequentis]
    #26126980 - 08/08/19 01:47 PM (4 years, 6 months ago)

Ya got a good point.  One test does not make for a scientific conclusion.  A part of me really hopes you succeed with this!

A thought to add to all this... A fruit that was simply air dried might do better than one cooked in a food dehydrator.

Another thought... instead of hydrating the fruit, perhaps a little of the dry inner tissue plopped on an agar plate might work.  The agar should hydrate the sample in a few hours.  Of course bacteria will proliferate, but if myc is present then it can be cleaned later.

Keeping on eye on your thread.  Interested to see how your subsequent attempts work out.


--------------------
Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"


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OfflineEl Chupacabra
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Registered: 01/07/19
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Re: Can you come from cracker dry tissue? Let's find out [Re: 1WithU]
    #26127007 - 08/08/19 02:07 PM (4 years, 6 months ago)

I'm still really interested for the science.
I know it hardly matters because its just so impractical. But its fun to try anyway. I will probably go at it again multiple times right there with ya.

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Offlinenoluckdude
El Duderino
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Registered: 05/10/19
Posts: 38
Last seen: 4 years, 2 months
Re: Can you come from cracker dry tissue? Let's find out [Re: verum subsequentis]
    #26127040 - 08/08/19 02:34 PM (4 years, 6 months ago)

Quote:

verum subsequentis said:
Not so fast friend. One of the biggest problems in mush cult is coming to conclusions too quickly. That's not how science works. I'll have to try this again and again before i make an official statement. It would appear that it doesn't work though.




It does in fact work. I took a dried stem and got myc growth. Hydrated pieces from inside the stem in sterile water and let sit until I saw myc growth(20+ days), then transferred to agar, then transferred 2 times. I just transferred it to grain, so we could see fruiting in 2 weeks :smile:

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Offline1WithU
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Re: Can you come from cracker dry tissue? Let's find out [Re: noluckdude]
    #26127083 - 08/08/19 02:57 PM (4 years, 6 months ago)

Hmmmm... multiple people saying this might or does work.  Hmmmm...

I like what you said noluckdude.  Maybe I will cut a cm of stem with scissors and put it in a baggie with a piece of wet cardboard instead of agar.  I have done lots of cloning with cardboard, and why is cool about it is that bacteria don't really grow.  If myc grows fuzz it'll be obvious and not fighting against contams.

Alright, I shall cut a piece and try it.  Will upload a pic soon.


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Wishing everyone awakened consciousness!
Explore the transformative benefits of microdosing.
"May my many blessings bless many others"


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Offlineverum subsequentis
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Re: Can you come from cracker dry tissue? Let's find out [Re: 1WithU]
    #26127111 - 08/08/19 03:12 PM (4 years, 6 months ago)

I'd strongly advise any one willing to try it out, document with procedures and pics and post them here. You could obviously start a new thread but i hate that information can be so hard to find around here because it's spread out through so many thousands of threads. Posting here would make it an all inclusive everything you need to know about cloning a dried fruit.

It is already assumed that doing this with an air dried fruit would work as it never reached temperatures high enough to actually kill the myc. That is kind of not the point though because anyone drying to store long term uses high heat (for the most part). The only time anyone would need to clone from an old dried fruit is if they were way out of the game and only had some old ass fruit lying around. that's how my brain sees it anyhow.

Noluckdude, word on the streets is "pics or it didn't happen". Was the fruit thoroughly dried at high temperatures? Do you have any pics? Would you mind doing it again and taking photos?

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InvisibleBoogieman47
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Registered: 03/05/16
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Re: Can you come from cracker dry tissue? Let's find out [Re: verum subsequentis]
    #26127126 - 08/08/19 03:18 PM (4 years, 6 months ago)

I would guess you would have senescence at the least ? I mean just from storing in the fridge with temp fluctuations not even freezes loses cultures ..and I have seen loss of vigor in temps at 120f ..  I enjoy all your experiments verum you amaze me with your dedication to our lifestyles


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Offlinenoluckdude
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Registered: 05/10/19
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Re: Can you come from cracker dry tissue? Let's find out [Re: verum subsequentis]
    #26127155 - 08/08/19 03:35 PM (4 years, 6 months ago)

The fruit was cracker dry at 165 dehydrator. I don't have any pics of the dried fruit, or the pieces in the sterile water growing myc. But I do have the agar plates still. Some were weird looking. I wouldn't recommend doing it. I did it just for fun to see if I could. I know I should have documented it more. Maybe I'll do it again when I'm not too busy. I can post photos of the agar plates, and I can post pics of the grain once it grows out if you are interested.

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Offlineverum subsequentis
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Re: Can you come from cracker dry tissue? Let's find out [Re: noluckdude]
    #26127174 - 08/08/19 03:47 PM (4 years, 6 months ago)

I'd be plenty interested but it wouldn't be that beneficial in the long run. Unless you stick around and develope a killer reputation as an amazing magic gatherer and honest dude, it'll just be pics of an agar plate and some grain that some dude says is from... You know what i mean. I'm not doubting or hating on you. I just remember learning the ropes and digging through threads. I did it a lot. I'm a serious nerd when it comes to figuring out how to do what it is i want to do. I got really really tired of post after post after post of some anonymous person, who i literally have no reason to trust or doubt, saying "i did this".

Even a write up like this one could be complete bullshit but the chances are far less great. I always figure, who in the fuck would take the time to pretend like they documented the fuck out of something in "real time"? And why.

I find full blown, well documented write ups with photos to be truly wonderful.

Thanks booger  man. I appreciate that and it's nice to read BUT, I'm really just in it for the money. I kid, I kid.  I honestly believe that the magic is way way way way way way more important than humans realize it is and i'm wanting to learn as much as possible about how it works. It's an addiction.

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InvisibleBoogieman47
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Re: Can you come from cracker dry tissue? Let's find out [Re: verum subsequentis]
    #26127407 - 08/08/19 05:44 PM (4 years, 6 months ago)

Watch zeitgeist it's pretty trippy and the magic brings out the thought process even more. Of how our world is ran which is why the magic was made illegal when it was

Whether money fun or just because, we do it is because it's what makes our minds turn as you started its addicting because we are the exception to reality of the sheep society has made


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