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Well done, I have isolated Pleurotus cultures from supermarket mushrooms in the past and had great results. The fresher the better.
When you have a nice fast growing pure culture on agar, transfer to sterilised rye grain in jars to make spawn. In 2 weeks you can then transfer grain spawn to bags of pPasteurised wheatstraw, and within another 2 weeks they should show signs of fruiting. Good luck.