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OfflineChumofChance
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First Time Agar - Moisture Surrounding Inoculation
    #26121471 - 07/21/19 12:09 PM (1 year, 7 months ago)

Hello, I am new to mushroom cultivation but have done lots of reading on here and on reddit. My first attempt to cultivate is from some dried gills (images 1 & 3) and from some very old (>4yrs) dried stem (image 2). My agar recipe is 5g tryptone, 2.5g yeast extract, 5g NaCl and 7.5g agar agar in 500mL water. For my first attempt, I saw no growth after 6 days (from the dried stem, so no surprise) but after 10 days there was white surrounding the stem piece and a few of bacterial colonies scattered on the dish. I had not used antibiotic in my agar, but seeing the bacteria, I poured the same recipe with 5mg of ampicilin.

I transferred what I thought was myc from the dried stem, and placed gills of fresher dried caps onto amp plates.

I can't tell what's building up on my agar. This is about 18hrs after inoculation onto amp-plates for all three. It looks like bacteria, but there shouldn't be any bacteria on here.

Thanks in advance.








Edited by ChumofChance (07/21/19 12:13 PM)


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Offlinek5hd2y
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Re: First Time Agar - Moisture Surrounding Inoculation [Re: ChumofChance]
    #26121496 - 07/21/19 12:16 PM (1 year, 7 months ago)

How did you sterilize ampicilin? Im not sure you can use heat with that one.  Maybe im wrong about the ampicilin, but i do see bacteria.


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OfflineChumofChance
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Re: First Time Agar - Moisture Surrounding Inoculation [Re: k5hd2y]
    #26121511 - 07/21/19 12:21 PM (1 year, 7 months ago)

Added amp after autoclaving - typical practice in molecular biology. For reference, here's a picture of the original one with what is certainly bacteria. I transfered a small section from 9oclock:



What's on these plates does not look the same as what was on this plate.


Edited by ChumofChance (07/21/19 12:22 PM)


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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