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 ChumofChance
Stranger
Registered: 07/03/19
Posts: 2
Last seen: 1 year, 7 months
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 First Time Agar - Moisture Surrounding Inoculation 
#26121471 - 07/21/19 12:09 PM (1 year, 7 months ago) |
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Hello, I am new to mushroom cultivation but have done lots of reading on here and on reddit. My first attempt to cultivate is from some dried gills (images 1 & 3) and from some very old (>4yrs) dried stem (image 2). My agar recipe is 5g tryptone, 2.5g yeast extract, 5g NaCl and 7.5g agar agar in 500mL water. For my first attempt, I saw no growth after 6 days (from the dried stem, so no surprise) but after 10 days there was white surrounding the stem piece and a few of bacterial colonies scattered on the dish. I had not used antibiotic in my agar, but seeing the bacteria, I poured the same recipe with 5mg of ampicilin.
I transferred what I thought was myc from the dried stem, and placed gills of fresher dried caps onto amp plates.
I can't tell what's building up on my agar. This is about 18hrs after inoculation onto amp-plates for all three. It looks like bacteria, but there shouldn't be any bacteria on here.
Thanks in advance.
Edited by ChumofChance (07/21/19 12:13 PM)
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k5hd2y
Depersonalized & Complete
Registered: 04/13/17
Posts: 675
Last seen: 1 year, 3 months
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 Re: First Time Agar - Moisture Surrounding Inoculation [Re: ChumofChance]
#26121496 - 07/21/19 12:16 PM (1 year, 7 months ago) |
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How did you sterilize ampicilin? Im not sure you can use heat with that one. Maybe im wrong about the ampicilin, but i do see bacteria.
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ChumofChance
Stranger
Registered: 07/03/19
Posts: 2
Last seen: 1 year, 7 months
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Re: First Time Agar - Moisture Surrounding Inoculation [Re: k5hd2y]
#26121511 - 07/21/19 12:21 PM (1 year, 7 months ago) |
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Added amp after autoclaving - typical practice in molecular biology. For reference, here's a picture of the original one with what is certainly bacteria. I transfered a small section from 9oclock:
What's on these plates does not look the same as what was on this plate.
Edited by ChumofChance (07/21/19 12:22 PM)
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