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Offlineatizva
habibii101
I'm a teapot


Registered: 05/18/19
Posts: 23
Last seen: 2 months, 1 day
Three questions about contamination in my plate.
    #26116756 - 07/18/19 07:40 PM (5 years, 5 months ago)

I've noticed a yellow appearance in all my rounds. I inoculated with spores directly from my spore syringe three weeks ago. The yellow is covered by the mycellium (Or at least I think it's mycellium and not cob-web). The green area is not covered yet by mycellium.



Here's a picture of this plate on the inside:



They haven't pin yet which does not look normal to me (but again I'm a beginner so I couldn't be sure). I wonder if this yellow appearance is normal, or is it due to to the mycellium producing enzyme to battle the contaminants ?

One of my posts from 4 days ago, in which I've asked if my rounds had clean mycellium, or is there obvious cob web contamination. Well, to know I was advised to do some transfers. The image below shows the plate I  transferred four days ago.



Which looks healthy to me with some rhizomorphic grow. Now I recall touching my agar with my sterelized blade various times (because the mycellium sample I isolated from the other round got stuck to the blade and I was battling to put it gently on the agar.)  This might be why I have two white spots (The obvious on the middle and the other at 1 o'clock). Noticed some bacteria too at 11 o'clock. But could anybody tell me what is the black dot at nine o'clock ?

Finally my plates look healthy to me. With some bacteria colonizing but that should be ok with one transfer. I can't see any cobweb in this round. But please let me now if you have noticed something else going on.
Thanks in advance :smile:

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InvisibleFunky Monkey
Human Suppository
 User Gallery


Registered: 05/14/19
Posts: 1,099
Loc: In your MOM's poop shoot
Re: Three questions about contamination in my plate. [Re: atizva]
    #26116806 - 07/18/19 08:16 PM (5 years, 5 months ago)

How it should and shouldn’t look.

Oh, and as always, the surest route to success is hours upon hours of reading the extensive amount of resources all of us shroomery members have available to us through the search function.

BODs easy AF search engine tek

:cheers:

Edited by Funky Monkey (07/18/19 08:17 PM)

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OfflineJe77Ce11ar
I'm a teapot


Registered: 01/09/19
Posts: 244
Last seen: 1 year, 3 months
Re: Three questions about contamination in my plate. [Re: Funky Monkey]
    #26117152 - 07/19/19 01:16 AM (5 years, 5 months ago)

The plates look healthIER but not healthy. Keep doing some more transfers and review sterile technique and how to prep your work space before you work with agar.

Good news is i see improvement

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