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OfflineNeonBruce
Stranger
Registered: 06/10/19
Posts: 13
Last seen: 4 years, 6 months
Is this LC trash?
    #26116535 - 07/18/19 05:34 PM (5 years, 5 months ago)

I'm new at all of this but I followed a pretty simple recipe for liquid culture

2 tbs dextrose
2 tbs malt barley extract
500 ml water
Microwaved for 2 x 3 minute intervals to steralize

Then I added a generous amount of slivers of mycelium on agar to the mix and have been agitating pretty constantly on a magnetic stirrer and I have no idea if it turned out well.  I just added some of this mix to some agar plates today to test whether it will be successful, a dud, or contaminated.  Does this look okay and if not could anyone give me some suggestions on what to do better.

I did all my work in a still air box made of a totes with arm holes cut in it.  Then I latex gloved up, rubbed my hands are arms with sanatizer, and then covered my hands and arms in spray disinfectant.  I used an exacto knife steralized on an alcohol lamp to cut the agar and fling it into the LC jar.

This jar of LC is about a week or 2 old.


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OfflineLyh
Mushroom Boi


Registered: 05/16/18
Posts: 296
Loc: Germany
Last seen: 1 month, 30 days
Re: Is this LC trash? [Re: NeonBruce]
    #26116569 - 07/18/19 05:55 PM (5 years, 5 months ago)

Quote:

NeonBruce said:

Microwaved for 2 x 3 minute intervals to steralize





I think that doesn't work this way.. also yeah cloudy LC is a no no.


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OfflineSubfinder
Wildlife enthusiast
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Registered: 07/01/18
Posts: 1,025
Loc: Pennsylvania
Last seen: 1 year, 9 months
Re: Is this LC trash? [Re: Lyh]
    #26117247 - 07/19/19 04:20 AM (5 years, 5 months ago)

If you microwaved your LC it's not sterilized. You will need to PC your LC and inoculate with clean myc.

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