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InvisibleProfessorSwan
Student


Registered: 05/27/19
Posts: 8
Loc: Paranoia
Galindoi ATL#7 — First Grow!
    #26096547 - 07/08/19 05:22 PM (5 years, 6 months ago)

Hello! This is a friend's first grow! They've done hours of stalking and research.

The friend has been following Stonesun and Violet's Truffle Teks. The Official Stone Thread was invaluable as well.

• A 12cc P. Galindoi ATL#7 spore syringe was used.
• Of 12 quart jars, 6 have SHIPs, and 6 have no SHIPS. All inoculations were done in a SAB. 1 jar was set aside un-inoculated to serve as a "control" in case of contamination.
• Hard Red Wheat Berries were used as the substrate, as they are pretty similar to Rye.


Image: My handiwork with the jars!


The grow is 18 days along! It took 9 days for all jars to show sign of mycelium. During the first 9 days, the jars had some pretty steep temperature fluctuations, ranging from 70°C up to 88°C. The second 9 days the jars were moved to a more stable environment, where temperature is maintained at a cool 72°C. The jars have access to ambient light from the room.

None of the jars seem to have any signs of contamination, which is making my friend really happy, considering how paranoid he was about his first grow! The jars have shown really good growth since they've all germinated, but the growth pattern seems to worry my friend. It seems like only half of each jar has been colonized. Furthermore, some of the jars even seem to be starting to develop stones, but the substrate isn't even completely colonized. Is this an issue, or is the friend just being impatient?


Image: The jar farthest along at day 9



Image: The same jar on day 18



Image: The other side of that same jar on day 18



Image: A different jar at day 18 that appears to have begun forming stones. This jar is not fully colonized.


I will be updating this thread/ update posts as the grow progresses. Right now I plan to let them sit for 3 months. I haven't shaken my jar, on fear of messing something up.

Is the growth slow? Please give some feedback and tips!

Edited by ProfessorSwan (07/08/19 05:32 PM)

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OfflineSvetaketu
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Re: Galindoi ATL#7 — First Grow! [Re: ProfessorSwan]
    #26096724 - 07/08/19 06:55 PM (5 years, 6 months ago)

Hey ProfessorSwan,

First off, if your friend is the one growing these, tell him to make an account and post this himself. We here grow mushrooms, and we do not SWIM :wink:


Anyway, all the jars look great to me! don't worry about the uncolonized halves, It'll get there eventually.
Shaking would probably make them colonize more evenly, and potentially a little faster, but I'm still cautious about shaking this species... out of the three test jars I shook, 1 recovered and fruited well, but the other two developed kind of a funky look, and never produced stones.

The growth speed looks about right (compared to my last batch). Be patient, and goodluck! :thumbup:


--------------------
LAGM2020
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InvisibleProfessorSwan
Student

Registered: 05/27/19
Posts: 8
Loc: Paranoia
Re: Galindoi ATL#7 — First Grow! [Re: Svetaketu] * 1
    #26097034 - 07/08/19 09:34 PM (5 years, 6 months ago)

Thanks for the warning about SWIM :tongue:. I thought I was being real clever, ha....

It's good to know that my jars are on track! Regarding the shaking, yeah, I'd definitely bare any extra waiting in order to minimize the chance of things going wrong. Thank you so much :smile:

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Offlinebadmojo420
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Registered: 05/27/13
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Re: Galindoi ATL#7 — First Grow! [Re: ProfessorSwan]
    #26097060 - 07/08/19 09:50 PM (5 years, 6 months ago)

following. i have a few atl7 jars going now that i think i am going to harvest and spawn to bulk in 3 weeks or so.


looks pretty good so far


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InvisibleUrbanCubensis


Registered: 05/13/18
Posts: 172
Loc: down the road
Re: Galindoi ATL#7 — First Grow! [Re: ProfessorSwan]
    #26097175 - 07/08/19 11:07 PM (5 years, 6 months ago)

That's awesome! Great work!

I just got an ATL#7 print not long ago also! Going to buy more jars this week and make up about 5-7 quarts of it!

I was wondering with this species, seeing as you can fruit it as normal as well as leave in jars to make stones, is the typical method of making stones just inoculate, colonise the jar and leave for about 2-3+ months? When done, do you just tip the jar out and sort through for the stones, then discard the rest? (I won't be throwing any in trash no matter what, all goes into my garden bed!

Just I've always wondered with this species, seeing as you can either make stones in jars or just fruit to normal for prints. When you fruit into say, a shoebox, does it produce stones under the sub while it fruits? I wouldn't say so, as it would be very little time compared to the 2+ months.

Thanks! I can't wait to get started on this beautiful species! (btw hey svetaketu! :wink: )


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Offlinebadmojo420
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Re: Galindoi ATL#7 — First Grow! [Re: UrbanCubensis] * 1
    #26097434 - 07/09/19 03:45 AM (5 years, 6 months ago)

i use agar and put it to my rye and just let it sit and its been making stones. i read you can take the stones out and use the rye to spawn with which is what im planning to do with mine in a few weeks


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OfflineSvetaketu
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Re: Galindoi ATL#7 — First Grow! [Re: badmojo420] * 1
    #26097815 - 07/09/19 09:59 AM (5 years, 6 months ago)

Hey Urban :wink:

So when you put the spawn into a bulk sub, it will try to produce more stones as it fruits. It won't have as much time to do so, but the stones are created a little faster in bulk subs than in jars.

On my last batch I waited 3-4 months, and started opening jars. I lost the genetic lottery when it came to stones, and even though they looked pretty large, all of my stones were extremely flat, making them weigh almost nothing.

So when I spawned those jars, I put the entire quart in the shoebox; stones and all.
They sat in the bulk sub for a little over a month, and by harvest most of the stones grew 2x - 3x bigger.

Now based on those experiences, I like to spawn the stones and everything. It's pretty common to find fruits shooting directly out of stones, which looks cool as fuck :awesome:
However, you will end up with whatever is in your bulk and casing, coating your stones a little bit.
This mostly doesn't matter at all, but I use a sand-based casing, and the stones near the surface often have sand that I can't fully clean, which is slightly unpleasnt to crunch into.

So really, it's up to you. If you are particular about perfectly clean stones, you may not want to spawn them. I've seen plenty of large stones pulled out of grain jars, and the leftover grain spawn will still produce fruits. However the stones WILL get substabtially bigger if they are put into a bulk sub.


--------------------
LAGM2020
LAGM2021

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InvisibleProfessorSwan
Student

Registered: 05/27/19
Posts: 8
Loc: Paranoia
Re: Galindoi ATL#7 — First Grow! [Re: Svetaketu]
    #26097894 - 07/09/19 10:50 AM (5 years, 6 months ago)

Quote:

badmojo420 said:
following. i have a few atl7 jars going now that i think i am going to harvest and spawn to bulk in 3 weeks or so.
looks pretty good so far




Quote:

UrbanCubensis said:
That's awesome! Great work!
I just got an ATL#7 print not long ago also! Going to buy more jars this week and make up about 5-7 quarts of it!





Thanks for the replies guys! Good luck with your grows, too.

I heard that you can let the jars sit for up to 12 months. My plan is to open up a couple jars every month, harvest the stones, then fruit the leftover grain. Like Svetaketu said, just moving the entire jar to bulk would probably yield bigger stones, but I haven't actually tried shrooms yet, so at least for the first jars I harvest I'm just gonna get at the stones right away :tongue:.

I was wondering, before I move the leftover substrate to bulk, can I use the colonized grain to inoculate another set of jars? It'd be nice to just have a seemingly infinite cycle where every time I harvest a jar, I use a little bit of the previous grain to get a new jar going.

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OfflineSvetaketu
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Re: Galindoi ATL#7 — First Grow! [Re: ProfessorSwan]
    #26098000 - 07/09/19 11:50 AM (5 years, 6 months ago)

Quote:

ProfessorSwan said:
I was wondering, before I move the leftover substrate to bulk, can I use the colonized grain to inoculate another set of jars? It'd be nice to just have a seemingly infinite cycle where every time I harvest a jar, I use a little bit of the previous grain to get a new jar going.




While theoretically this would work, it becomes more complicated in reality. By the time the stones are large enough to harvest, the grains will be so thickly colonized by mycelium that they will be difficult to remove. Shaking is likely to do nothing at this point, and to remove the stones you may have to get a spoon and break up the spawn manually.

This is all fine and dandy if you're spawning them, because colonized grains can withstand contamination very well. However, once you touch the grains they will be theoretically "contaminated" and spawning them to sterile grains will result in mold.

Technically you could wrap a spoon in tinfoil, sterilize it in a PC, and try to carefully remove the grains in a SAB without contaminating them, but this seems like altogether too much effort IMO.


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LAGM2020
LAGM2021

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Offlinebadmojo420
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Registered: 05/27/13
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Re: Galindoi ATL#7 — First Grow! [Re: Svetaketu]
    #26099174 - 07/09/19 09:05 PM (5 years, 6 months ago)

you could probably put a piece of sclerotia on agar and make the necessary transfers to clean it up then use that for grain instead of trying to G2G with the grains.

maybe even a piece of colonized grain?


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InvisibleProfessorSwan
Student

Registered: 05/27/19
Posts: 8
Loc: Paranoia
Re: Galindoi ATL#7 — First Grow! [Re: badmojo420]
    #26099232 - 07/09/19 09:31 PM (5 years, 6 months ago)

Quote:

Svetaketu said:
Technically you could wrap a spoon in tinfoil, sterilize it in a PC, and try to carefully remove the grains in a SAB without contaminating them, but this seems like altogether too much effort
IMO.




Quote:

badmojo420 said:
you could probably put a piece of sclerotia on agar and make the necessary transfers to clean it up then use that for grain instead of trying to G2G with the grains.
maybe even a piece of colonized grain?




Hm, at least in theory it sounded nice! I think you're right badmojo, agar with a piece of colonized grain definitely seems to be the way to go.

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OfflineSvetaketu
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Re: Galindoi ATL#7 — First Grow! [Re: ProfessorSwan]
    #26099283 - 07/09/19 10:01 PM (5 years, 6 months ago)

Colonized grain to agar should work great.

you can also take a stone, break it in half, and take a tissue sample from the inside to put on agar. This has the advantage of cloning the genetics of that stone, and guaranteeing "stone producing genetics" as some cultures may refuse to make stones, or only very small ones.

The same can be done with the fruits, although the size makes it a little tricky.


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Offlinebadmojo420
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Re: Galindoi ATL#7 — First Grow! [Re: Svetaketu]
    #26099291 - 07/09/19 10:04 PM (5 years, 6 months ago)

oh i have heard that as well. some isolates might not produce stones. i also read that when selecting transfers you should select a dark portion instead of a regular mycelial section.

is there any truth to that? it sounds plausible as the browning and darkening is the same color as the darkening adn browning on my stones so surely those areas are stone producing genetics?

or am i off base with that? i know not everything just adds up like that in mycology.


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OfflineSvetaketu
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Re: Galindoi ATL#7 — First Grow! [Re: badmojo420]
    #26100240 - 07/10/19 12:43 PM (5 years, 6 months ago)

That sounds plausible to me? Although I don't have enough experience with these guys yet to say for certain.

That being said, I do have one clone that I took from a fruit that was growing directly out of a stone, and the mycelium does go brown much faster than previous ones. In fact, it seems to make a lot of strange colors, and it has created at least one stone per petri its been in.


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LAGM2021

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Offlinek5hd2y
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Re: Galindoi ATL#7 — First Grow! [Re: Svetaketu]
    #26100276 - 07/10/19 01:04 PM (5 years, 6 months ago)

Quote:

Svetaketu said:
That sounds plausible to me? Although I don't have enough experience with these guys yet to say for certain.

That being said, I do have one clone that I took from a fruit that was growing directly out of a stone, and the mycelium does go brown much faster than previous ones. In fact, it seems to make a lot of strange colors, and it has created at least one stone per petri its been in.




This is a very interesting mycelium to play with for sure. Definitely a fun time watching it in plates. I get like an orange lumpy twist as it turns into a stone some are pushing on the lids.

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