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Offlinethe_sonic16
Mushy McMusherson
I'm a teapot User Gallery


Registered: 03/10/19
Posts: 396
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Re: Spore syringe on agar [Re: Yasuke]
    #26077780 - 06/27/19 05:18 PM (4 years, 6 months ago)

I took the Tormato approach (kind of).

I shook up the syringe and let as much of the visible spores settle as possible. I flamed and squirted a little out while still holding it vertical. I then placed individual droplets one at a time in concentric circles, until the plate was covered in separate circles.

I had to store it with the agar on the bottom, which I know is a no no because of condensation. Luckily, I didn't have any condensate, so it wasn't an issue and I was able to let the drops evaporate and ultimately germinate.


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Sonic's "I'll buy a pressure cooker someday" Instant Pot times and settings:
  • Popping Corn Rapid Re-hydration - Half fill all containers with corn. Empty into Instant Pot. Cover with water. Run 30 minutes on PC. Release, remove, dry.
  • Wheat/Rye Berry Soak Re-hydration - Half fill all containers with grain. Empty in pot with water. Soak for 6-12 hours, air dry until surface is completely dry.
  • Grain Jar Sterilization - Run 2 - 2.5 hrs on PC
  • PastyPlates, MEA Formulation- Run 45 minutes on PC
  • LME Liquid Culture - Run 45 minutes on PC


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