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OfflineYasuke


Registered: 05/10/19
Posts: 28
Loc: SoCal
Last seen: 4 years, 3 months
Re: Spore syringe on agar [Re: k5hd2y]
    #26077721 - 06/27/19 04:38 PM (4 years, 6 months ago)

Quote:

k5hd2y said:
Doubt it but possible. Make more of it and use an empty plate next time to put your wet spores >loop >agar :goodluck:



I one up this. I was having a horrible time with syringe to agar, and once I started just squirting a tiny bit of the inoculant on the inoculation loop and scraping it on agar my success rate went up phenomenally. I dont know if it was the less spore solution or what, but I'm gonna stick with it. I'd have thought the extra contamination factor wouldve made it tougher on me, but nope.


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OfflineYasuke


Registered: 05/10/19
Posts: 28
Loc: SoCal
Last seen: 4 years, 3 months
Re: Spore syringe on agar [Re: k5hd2y]
    #26077793 - 06/27/19 05:23 PM (4 years, 6 months ago)

Quote:

lindens said:
oooo i'll try this as well, i have some fresh loops. When you get it red hot, would a minute suffice for it to cool down before putting some solution on the loop?



I put 2 drops right after I flame sterilize it. The first drop is definitely enough to cool it down.
You probably wont be able to see anything on the agar when you inoculate it besides maybe some scratch marks, but its there.


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