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Offlinehexkrak
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Registered: 12/26/02
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Re: Spore syringe on agar [Re: lindens]
    #26077713 - 06/27/19 04:34 PM (4 years, 6 months ago)

Quote:

lindens said:
Thanks a lot! Will set up a few more plates with 1 drop of the syringe this time, and shake up that syringe to disperse the spores.

Another question, the agar i am using was made about 2 months ago (haven't had to chance to move forward until now), pressure cooked and kept sealed in a pyrex bottle (then gelled it up again in a microwave), is it possible it could've expired and is a source of the bacteria?



Depends on the seal on your bottle. That and I'm assuming you didn't leave it sealed when you heated it up in the microwave, so there's that too. However, in this scenario, you're seeing tons of surface bacteria, if it was contaminated in the bottle you'd see bacterial growth throughout the dish, and not just on top. This most likely came from the syringe or happened somewhere during the inoculation/storage process.


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