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Re: Spore syringe on agar [Re: lindens]
    #26077649 - 06/27/19 04:07 PM (4 years, 6 months ago)

Quote:

lindens said:
Using spore syringes of GT on agar, this is the result after 4 days.
I'm not sure how much you're supposed to squirt onto the agar but i wanted to get one of those black dots in each dish as seen in the first pic:

in the second pic i wasn't so lucky, only the clear liquid of the syringe made it in:

Are the black dots the actual GT spore? Do these look fine or is it too early to tell?



Those plates are nothing but bacteria! :uhoh:

One drop of spore syringe solution is plenty!


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Re: Spore syringe on agar [Re: lindens]
    #26077701 - 06/27/19 04:29 PM (4 years, 6 months ago)

A trick I do with spore syringes is after I shake em up real good I try to let some of it settle down near the needle hole....probably doesn't do shit, but it seems to work for me :lol:

Flame sterilize then hold the needle down and let the spores settle then...(drip) :thumbup:


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Re: Spore syringe on agar [Re: lindens]
    #26077800 - 06/27/19 05:27 PM (4 years, 6 months ago)

Quote:

lindens said:
oooo i'll try this as well, i have some fresh loops. When you get it red hot, would a minute suffice for it to cool down before putting some solution on the loop?



A loop will pretty much be cold enough to touch seconds after you remove it from heat. No need to wait


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